With the extensive application of informationalized management systems for barcode specimens , the degree of informatization is becoming higher and higher in medical laboratory.Informationalized management combines modern information technology and advanced management concepts , transforms or reengineers the laboratory operation and business process.Test specimen turnaround time ( TAT) is an important factor affecting the quality of the inspection.By analyzing the test process of each time node , establish the suitable specimens monitoring program for clinical requirements and real -time monitor the key nodes in test processes ,which will effectively shorten TAT , improve reporting timeliness rate and avoid clinical complaints.

Objective To compare the effects of salmeterol and fluticasone propionate combined montelukastin the treatment of patients with bronchial asthma.Methods Ninety-eight bronchial asthma patients from April 2014 to March 2016 in the Fourth People's Hospital Sichuan Province were selected anddivided into observation group and control group with 49 patients in each group according to the above principles by prospective study.The control group was given salmeterol treatment,and the observation group was added given montelukast treatment based on the control group.The treatment days were 15 d recorded the prognosis of the two groups.Results The total effective rates of the observation group and the control group were 95.9％ and 81.6％ respectively,the total effective rate of the observation group was significantly higher than that of the control group (P ＜ 0.05).The FEV1 and FVC values after treatment in the observation group and control group were significantly higher than before treatment (P ＜ 0.05),while the FEV1 and FVC values in the observation group after treatment were significantly higher than those in the control group (P ＜ 0.05).The scores of ACT scale after treatment of two groups were obviously higher than before treatment,and the score of observation group was statistically higher than control group after treatment (P ＜ 0.05).During treatment,there was no statistical significance on adverse reaction between two groups.Conclusion Salmeterol and fluticasone propionate combined montelukast in the treatment of patients with bronchial asthma can promote the improvement of lung function,so as to improve the quality of life and clinical treatment.

Background and purpose:Retinoic acid-induced gene G (RIG-G) is a tumor suppressor gene which is cloned by NB4 cell line from a acute promyelocytic leukemia cell. This study aimed to investigate the effect ofRIG-G in lung cancer cells A549 by constructing a lentiviral vector expressing RIG-G under doxycycline (DOX) regulation.Methods:RIG-G gene ampliifcation was performed by quantitative real-time PCR (qRT-PCR). pLenti6/TO/V5-GIM-RIG-G lentiviral vector withGFP was built by LR recombination system. The concentration of pLenti6/TO/V5-GIM-RIG-G lentiviral vector andTet-on lentiviral vector were measured by virus titer method. After infecting A549 cells, stably transfected lines were selected via limiting dilution analysis.RIG-G gene expression was examined by immunolfuorescence staining and Western blot assay. Cellular proliferation was determined by CCK-8 assay.Results:The concentrations of pLenti6/TO/V5-GIM-RIG-G lentiviral vector andTet-on lentiviral vector were 1.0×108TU/mL and 4×109 VP/mL, respectively. RIG-G was expressed in lentivirus infected A549 cells after adding DOX, and the amount of cells withGFP could be observed by lfuorescence microscopy.After the expression of RIG-G protein, the prolif-eration activity of A594 cell was signiifcantly inhibited compared to the control group (1.168±0.107vs 2.099±0.162, P<0.05).Conclusion:The regulated expression ofRIG-G gene was established in A549 lung cancer cell line. The RIG-G protein has potential abilities to inhibit the proliferation of lung cancer cell A549.

0.05). CONCLUSION The sound localization apparatus can test the localization ability of person, and it can be used in simply and reasonably.

BACKGROUND:No studies currently focus on the measurement of daily water loss under certain physical activity level continuously and precisely.
OBJECTIVE:To provide evidence for daily water requirement in Chinese young men based on the measurement of the daily water loss in Chinese young men under moderate physical activity.
METHODS:Twenty male subjects were enroled, and 23-hour whole-body sweat loss and urine output were measured using high-precision body weighting scale in whole-room indirect calorimeter. The results were converted to daily (24-hour) water requirement to determine the daily water requirement. Daily whole-body sweat loss per unit body weight, daily whole-body sweat loss per unit fat free mass and daily whole-body sweat loss per unit surface area were calculated.
RESULTS AND CONCLUSION:In Chinese young men, daily water loss was (3 339.7±509.9) g, daily urine output was (1 982.9±449.5) g and daily whole-body sweat loss was (1 356.7±215.6) g. There was a linear correlation between water intake and water loss (r=0.616,P < 0.05). Daily whole-body sweat loss per unit body weight was (21.0±3.6) g/kg, daily whole-body sweat loss per unit fat free mass was (25.8±3.9) g/kg and daily whole-body sweat loss per unit surface area was (799.4±129.4) g/m2. Daily water requirement of Chinese young men under moderate physical activity is approximately 3 340g, and moreover, water loss increases with the increase of water intake.

Objective:To explore the distribution of pathogen and the characteristics of drug resistance in gerontal patients with chronic obstructive pulmonary disease(COPD)accompanied by pulmonary infection,so as to provide the basis for rational usage of antibiotics.Methods:The sputum of 512 gerontal patients with COPD accompanied by pulmonary infection was collected for bacteria culture.The pathogenic distribution and drug sensitivity were analyzed.Results:There were 278 positive samples, with the positive rate of 54.3%.Of them,204 strains were gram-negative bacteria,including Pseudomonas (62 strains, 22.3%),Klebsiella (44 strains,15.8%),Haemophilus influenza (38 strains,13.7%),Enteric bacilli (34 strains,12.2%), and Acinetobacter (26 strains,9.4%).And 66 strains were gram-positive bacteria,including Staphylococcus aureus (32 strains,11.5%),Streptococcus pneumoniae (22 strains,7.9%),Enterococcus (12 strains,4.3%).Eight strains were fungi, including Candida albicans (6 strains,2.2%)and Yeast-like fungi (2 strains,0.7%).The rate of gram-negative bacteria was significantly higher than those of gram-positive bacteria and fungi(P <0.01).Gram-negative bacilli were most sensitive to mer-openem and imipenem,while they showed strongest drug resistance to ampicillin/sulbactam and amoxicillin.Gram-positive cocci were most sensitive to vancomycin and doxycycline,while they showed strong drug resistance to penicillin,erythromycin,clinda-mycin,levofloxacin,etc.The fungus had no resistance to flucytosine and fluconazole,ltraconazole and amphotericin B.Conclu-sions:The gerontal patients with COPD accompanied by pulmonary infection are mostly affected with gram-negative bacteria, which have high levels of resistance to antibiotics.According to experience,it is appropriate to choose the third and fourth generation cephalosporins or the compound antibiotics containing beta lactamase inhibitor for empiric treatment.

This paper is mainly devoted to the relationship between the correlation dimension (CD) of exercise heart rate variability (EHRV) and the status of cardiovascular function. Hypertensive patients and healthy people were enrolled in two contrast groups. Dynamic electrocardiograph (ECG) in step exercise was recorded. EHRV was extracted by wavelet transform. The CDs in the whole course of exercise and in three stages of exercise were calculated, the three stages being named rest-before-exercise, during-exercise, and after-exercise. SPSS software was used for statistical analysis. The results revealed all the CDs showed significant difference between two groups except that in the stage of during exercise. By discrimination analysis, the average correct rate was 92.2%. It indicated that the CD in stress status probably could be an effective non-linear parameter for assessing cardiovascular function status.
Adult ; Aged ; Case-Control Studies ; Electrocardiography ; methods ; Exercise ; physiology ; Exercise Test ; Female ; Heart Rate ; physiology ; Humans ; Hypertension ; physiopathology ; Male ; Middle Aged ; Nonlinear Dynamics ; Signal Processing, Computer-Assisted ; Young Adult

OBJECTIVETo investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development.

METHODSSpent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer (IVF-ET) cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed.

RESULTSIn the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample (93.8%) with a mean level of 0.85 ± 0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased (F=2.273, P=0.03), and decreased as the morphological grade of the embryo was lowered (F=3.900, P=0.02).

CONCLUSIONELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.

Biomarkers ; chemistry ; Chorionic Gonadotropin ; chemistry ; Culture Media ; chemistry ; Embryo Transfer ; Embryonic Development ; Fertilization in Vitro ; Humans

Objective:To investigate the application and effects of WeChat official account in improving the nursing trainees' core post competency.Methods:The study included in 105 nursing trainees of Batch 2015 as experimental group, taking the nursing practice WeChat official account based on the routine clinical teaching pattern, and 95 nursing trainees of Batch 2014 as control group, taking traditional teaching mode. At the end of the internship, the scores of special examinations for nursing students and the national examination for nursing qualifications ("nursing qualification examination" for short) of the two group were compared, and the critical thinking ability, ability to acquire information lifelong learning ability, nurse-patient clinical communication ability and teaching satisfaction of the two groups of students were analyzed. SPSS 23.0 software was used for t test. Results:The scores of special examinations, nursing qualification examination and each module at main dimensions of the core post competency of nursing students in the experimental group were higher than those in the control group ( P<0.05). The nursing trainees in the experimental group were satisfied with the teaching effects of the WeChat official account. Conclusion:The WeChat official account in clinical nursing practice helps improve the quality of clinical practice teaching and assist in enhancing core post competency of nursing trainees.

Objective To investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development. Methods Spent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer (IVF-ET) cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed. Results In the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample (93.8%) with a mean level of 0.85 ± 0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased (F=2.273, P=0.03), and decreased as the morphological grade of the embryo was lowered (F=3.900, P=0.02). Conclusion ELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.