Objective:To study the effect of hydrocortisone (HC) on apoptosis and expression of apoptosis- associated proteins in the intestinal epithelium of fetal mice.Methods:The duodena and colons were taken from E13.5d and E14.5d fetal mice respectively,and cultured with different doses of HC (0 ?g/ml,0.5?g/ml,1.0?g/ml,2.0?g/ml).After 24h,the samples were sliced up into the paraffin sections.Some sections were stained with HE to count the numbers of apoptotic bodies (NAB)in the fetal intestinal epithelium with stereological method.Others were stained with the immunohistochemical method to observe the expression of Bcl-2,Bax,P53 and c-Fos.Results:①In each experimental group,the NAB were higher than that of control group( P

Objective:To study the effect of methylmercury chloride(MMC) on the apoptosis and the expression of apoptosis-associated proteins in the intestinal epithelium of fetal mice.Methods:The duodenum and colon were taken from E13.5d and E14.5d fetal mice respectively,and cultured with different doses of MMC(0 ?g/L,200?g/L,400?g/L,800?g/L) for 24h.The samples were sliced into the paraffin sections.Some sections were stained with HE to count the numbers of apoptotic bodies(NAB) in the fetal intestinal epithelium with stereological method.The others were stained with the immunohistochemical method to observe the expression of apoptosis-associated proteins Bcl-2,Bax,P53 and c-Fos.Results:①In experimental groups,all the NAB in the intestinal epithelium were higher than that in control groups(P

Objective By mapping specific expression of RIM3? mRNA in the central nervous system and inhibitory neurons,we probed into physiological function of RIM3?. Methods Adult Wistar rats were used in the present study.The brain and cervical enlargement of the spinal cords of the adult Wistar rats were dissected.Using In situ hybridization and double labeled fluorescence assay,we demonstrated the specific expression of RIM3? mRNA in the central nervous system and inhibitory neurons. Results RIM3? mRNA was preferentially expressed in lateral posterior thalamic,mitral layer of the main olfactory bulb,layers Ⅱ-Ⅳ of somatosensory cortex,the granula layer of the cerebellum and the superficial layers of the spinal cord dorsal horn.Double labeled fluorescence assay showed the expression of RIM3? in some inhibitory neurons of the cortex,the spinal cord and the cerebellum of adult Wistar rats.Conclusion Specific expression of RIM3? mRNA in the CNS of rats provides the basis for further exploring physiological function of RIM3?.

Objective: To conquer the blindness in the management of hospital major medical equipment acquisition and the randomicity of instrument maintenance and repair;and establish a complete and scientific management system. Methods: study and draw conclusion on the complexity, constraint, innovation and purposefulness rules and characteristics of equipment management in line with the expected demands and determined purpose. Results: establish the mode of management by project for major medical equipment management, and improve the equipment use benefit. Conclusion:Perform the mode of management by project for major medical equipment management, and provide hospital major medical equipment management with new method by the on-going system quality control.

Objective To explore how to increase the production and improve the quality of collagen protein from cod skin to offer reference for exploition and utilizing fisheries waste material accordingly.Methods The collagen protein from cod skin was extracted by pepsin methods primarily.Results and conclusion A comparison of the productions of collagen protein from different additions of pepsin,we suggested that the optimum addition of pepsin was 1%(w/w) and the optimum ratio between cod skin and solvent was 1∶10.

Objective To observe the effects of peridural and general anesthesia on haemodynamics in gynecological laparoscopic operation.Methods 106 patients with gynecological laparoscopic operation were randomly divided into epidural anesthesia group(n=53)and general anesthesia group(n=53)respectively.The changes of heart rate(HR),systolic blood pressure(SBP),diastolic blood pressure(DBP),SPO_2 were monitored before anesthesia,at 20min after pneumoperitoneum(pp),end of operation.Results The sober time in epidural anesthrsia geoup(7.1 ±2.5)min was significantly faster than that in general anesthesia group(20.8 ± 6.3)min(t=2.068,P＜0.05).At 20min after pp,HR in general anesthesia group(91 ±17)was significantly faster than that in ppidural anesthesia group before anesthesia(t=2.593,P＜0.05),and it was significantly higher than epidural anesthesia group(t=3.417,P＜0.05).SBP in epidural anesthesia group was singnificantly lower than before anesthesia(t=2.806,P＜0.05),and it was significantly lower than general anesthesia group(t=3.917,P＜0.05).Conclusion General anesthesia does not significantly better than epidural anesthesia in gynecological laparoscopic operation,but they should enhance the clinical monitoring of hemodynamics.

Objective To construct competency models of health nurses. Methods The compe-tency models of health nurses based on eompetencies of health nurses by questionnaires survey of check-list and Behavioral Event Interview (BEI) was obtained. First, questionnaires survey of checklist was ad-ministrated and based on these results, coding competency dictionary was obtained through focus group discussions of professionals. Then the transcripts of BEI were coded on the basis of this competency dic-tionary. Results Significant difference existed in time and length of interview. The excellent nurses and common nurses were different in responsibility, professional knowledge and technique, initiative, emergency capability and rigourism. Conclusions The four factors, including service characteristic, cognitive style, professional particularity, tendency particularity, construct the competency models of health nurses.

OBJECTIVE:To explore the situation and relevant factors of ADR induced by Shengmai injection and find out reason and preventive measure of ADR in order to provide reference for rational use of drugs. METHODS:60 ADR cases of Shengmai injection published from 1989 to 2009 were analyzed in terms of age distribution,organs and systems involved in ADR and its manifestation. RESULTS:ADR cases induced by Shengmai injection mainly were allergic reactions(including rash,urticaria,conjunctival edema,fever,chills,allergic shock),34 cases accounting for 56.67%,followed by gastrointestinal system,7 cases accounting for 11.67%.7 cases of cardiovascular system accounted for 11.67%,other manifested as cardiovascular system response, visual impairment and local pain,etc.CONCLUSION:Clinical staff should be aware of the characteristics of ADR induced by Shengmai injection and its pathogenesis and grasp medication time as well as preventive measures. Drug use should be standardized to avoid and decrease the incidence of ADR.

OBJECTIVE:To study the in vitro and in vivo metabolism of DX by intestinal bacteria in rats and volunteers and the active components in serum of rat after oral administration of DX 900mg/kg METHODS:To detect DX and its metabolites in stool,urine and serum with TLC and EST-MS RESULTS:In vitro,DX was decomposed easily by rat and human intestinal bacteria,and various metabolites were found With prolongation of metablism,metabolite with molecular weight of 415 3 was shown to be corresponding to diosgenin in rat serum and in urine of rats and volunteers CONCLUSION:The above-mentioned DX was decomposed easily by intestinal bacterias and diosgenin was absorbed into serum after oral administration of DX

Senile dementia is a common degenerative disease of center nervous system in old age.The pathogeny and pathomechanism haven't been made clear.And the precise therapy is lack.It is thought that the Heart and Spleen has physiological and pathological relevance with brain by analyzing the relation between the Heart and Spleen and senile dementia in the article.Using method of benefiting the Heart and Spleen in treating senile dementia not only improves the somatic symptoms,also delays the degeneration of intelligence of the patients.The point puts forward the new thinking and method to cure senile dementia.