Objective To investigate the application value of 16S rDNA-based detection technique in the rapid screening for and confirmation of gonococcemia. Methods A 41-year-old male patient was hospitalized for recurrent regular fever and chills for 1 month. Several days before the admission, he developed urgent micturition, frequent micturition and pain in urination, anemia with emaciation appearance, slightly pale-looking skin and mucous membranes. No petechia, skin eruption or superficial lymphadenectasis was observed, but routine blood test and urine test results were abnormal. No abnormality was found in stool test or hepatic and renal function. DNA was extracted from the blood of the patient and subjected to PCR for the amplification of 16S rDNA followed by sequence analysis and homology analysis. At the same time, bacterial culture of blood and drug sensitivity test for the bacterial isolate were performed. Results Homology analysis indicated that the amplicon sequence was consistent with the known sequence of 16S rDNA of Neisseria gonorrhoeae in GeneBank, which agreed well with the culture result of peripheral blood. Conclusion The detection of 16s rDNA with PCR from peripheral blood is highly efficient, specific, sensitive, rapid and accurate for the screening for and confirmation of gonococcemia.

The inhibitory effect of hydroxyapatite ultrofine powder (HAUFP) on tumor and the effect on the immunity function of body were investigated. The levels of IL-2 in the spleen cells and serum TNF levels in the tumor-bearing mice at the 7th day and 14th after peritoneal injection of HAUFP were detected by using the methods of colorimetric analysis of MTT and crystal purple decoration, respectively. The disappearance of the ascites of the mice was observed. The results showed that the levels of IL-2 and TNF in the tumor-bearing mice were higher obviously in the drug-treated group than in the control group (P<0.01), the ascites growth was inhibited. It was suggested that HAUFP could increase the levels of IL-2 and TNF of the tumor-bearing mice and improve the immune function of body.

The inhibitory effect of hydroxyapatite ultrofine powder (HAUFP) on tumor and the effect on the immunity function of body were investigated. The levels of IL-2 in the spleen cells and serum TNF levels in the tumor-bearing mice at the 7th day and 14th after peritoneal injection of HAUFP were detected by using the methods of colorimetric analysis of MTT and crystal purple decoration, respectively. The disappearance of the ascites of the mice was observed. The results showed that the levels of IL-2 and TNF in the tumor-bearing mice were higher obviously in the drug-treated group than in the control group (P<0.01), the ascites growth was inhibited. It was suggested that HAUFP could increase the levels of IL-2 and TNF of the tumor-bearing mice and improve the immune function of body.

OBJECTIVE：To know about the use of policy tools for trad itional Chinese medicine （TCM）inheritance and innovation policies in China ，and to provide reference for subsequent improvement of TCM inheritance and innovation policies. METHODS：The content analysis method was used to classify and code the policy texts related to TCM inheritance and innovation policies at the national level before Sept. 2020. Statistical analysis was carried out from the two dimensions of basic policy tools and the TCM inheritance and innovation system ，and related suggestions were put forward. RESULTS ：Nine related policy texts were collected ，which were published during 2007-2020. In terms of basic policy tool dimensions ，environmental policy tools were the most （46.47％），followed by supplying policy tools （41.36％）and demanding policy tools （12.17％）. The supply-based policy tools included scientific research and informatization support （25.67％），talent training （7.06％），infrastructure construction （5.6％），public health services （2.07％），and capital investment （used the least ，0.97％）. The demand-based policy tools included international exchanges and cooperation （5.11％），organization coordination （3.77％），industry shaping （1.82％），pilot/ demonstration projects （1.46％），the latter two contents were used less. Among the environmental policy tools ，laws and regulations were used the most （24.94％），followed by target planning （9.73％），intellectual property （6.57％），financial support （2.43％），policy publicity （1.95％），quality evaluation （0.61％），and medical insurance support （0.24％）. The dimensions of TCM inheritance and innovation system included scientific research and innovation （34.67％），management mechanism （19.59％）， Chinese medicine industry （18.37％），service system （18.00％）and team building （9.37％），among which team building was related less. CONCLUSIONS ：In order to better implement TCM inheritance and innovation policies in China ，it is recommended to optimize supply-based policy tools and highlight policy promotion functions ；increase demand-based policy tools to form an effective“pull force ”；adjust the structure of environmentalpolicy tools to improve the external environment for inheritance and innovation ；promote the full use of policy tools in the inheritance and innovation system to achieve planning goals.