Objective To investigate the role of hMSH2 in the pathogenesis of sporadic insulinomas and to determine whether the expression of hMSH2 could be used to differentiate benign sporadic insulinomas from malignant ones. Methods Fifty-five sporadic insulinomas (40 benign and 15 malignant tumors) resected from 50 patients were obtained. Expression of hMSH2 was detected by immunohistochemistry staining. DNA was obtained from micradissected tissue. Loss of heterozygnsity (LOH) of hMSH2 gene was detected by PCR-LOH. 6 microsatellite markers were selected on 3 chromosomes, and microsatellite instability (MSI) status of tumor tissue were detected by PCR. The findings were analyzed in relation to the clinicopathological characteristics. Results Down-regulation of hMSH2 expression was found in 13% of 55 sporadic insulinomas. LOH of the hMSH2 gene was not present in 55 insulinomas. High frequency MSI (MSI-H, MSI occurred in at least 2 out of 6 sites) was present in 36% (20/55) of all the insulinomas. Down-regulation of hMSH2 expression was found in 33% of the 15 malignant tumors, while it was 5% in benign tumors (P < 0. 05). Conclusions Down-regulation of mismatch repair gene hMSH2 may be correlated with the degree of tumor malignancy. The expression of hMSH2 could be used as a potential marker for distinguishing benign insulinoma from malignant ones.

Objective To explore the application effect of the table format shift sheet on the shift handover among the intra-aortic balloon pump ( IABP) implantation patients .Methods The important contents of shift handover in IABP implantation patients were listed in the table format shift sheet , and the shift sheet was used in the process of shift in cardiac care unit ( CCU) for the patients with IABP implantation .Results The integrity rate of the shift content and the nurses ’ assurance rate of understanding the patients ’ conditions were respectively 100%and 95.0%after the application of the table format shift sheet , and were 71.6%and 70.0%before the application , and the differences were statistically significant (χ2 =35.80,17.32, respectively;P<0.01 ) .The time of shift after the application was shorter than that before the application , and the difference was statistically significant (P <0.01).Conclusions Application of the table format shift sheet including the important data and details of shift content in patients with IABP implantation can make the nurses grasp the key points of shift during the shift handover , and shorten the time of shift , and improve the integrity of the shift content, and enhance the nurses ’ assurance of understanding the patients ’ conditions, and increase the quality of shift in CCU .

Objective To analyze effects of nasal tube of oxygen inhalation and mask double oxygen inhalation, on thoracic operation patients complicated with pulmonary infection. Methods A total of 66 thoracic operation patients complicated with pulmonary infection were randomly divided into nasal tube group (33 cases, nasal tube of oxygen inhalation therapy) and double group (33 cases, mask double oxygen inhalation therapy) according to random number table method. Therapy effects were explored in two groups. Results PaO2 of patients in double group was higher than that in nasal tube group 30, 60, 90 minutes after oxygen inhalation ( P<0. 05). Average respiratory rate was (56. 23 ± 4. 15)/minute in double group 60 minutes before and 60 minutes after oxygen inhalation, which was significantly higher than that in nasal tube group (44. 82 ± 5. 64)/minute. SpO2 patients in double group was also significantly higher than that in nasal tube group (P<0. 05). There was no significant difference in the incidence of adverse reactions between two groups (χ2 =0. 569, P>0. 05). Conclusions Application of nasal tube oxygen inhalation and mask double oxygen inhalation can obviously improve the treatment effectiveness of thoracic operation patients complicated with pulmonary infection and do not increase the total incidence of side effects.

OBJECTIVE To establish the fingerprint of Pinghuo tea standard decoction and a method for determination of multi-component to clarify the transfer relationship of quantities and quality from pieces and standard decoction. METHODS Fifteen batches of Pinghuo tea standard decoction were prepared and the extract rate was determined； the fingerprint of the preparation was established by using high-performance liquid chromatography（HPLC）； the similarity evaluation and the determination of common peaks were performed， and chemometric analysis was performed； the same method was used to determine the content of indicator components and the transfer rate was calculated. The chromatographic column was Venusil C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution （gradient elution）； the column temperature was 30 ℃， and the detection wavelengths were 238 nm （0-37 min， 85-102 min） and 330 nm （37-85 min） at a flow rate of 1.0 mL/min with an injection volume of 10 μL. RESULTS The similarity of HPLC fingerprints for 15 batches of Pinghuo tea standard decoction was not lower than 0.968. A total of 24 common peaks were calibrated and 9 peaks were recognized， which were as follows neochlorogenic acid （peak 3）， chlorogenic acid （peak 6）， geniposide （peak 9）， glycyrrhizin （peak 10）， galuteolin （peak 11）， isochlorogenic acid A （peak 14）， luteolin （peak 21）， kaempferol （peak 23） and glycyrrhizic acid （peak 24）. Cluster analysis， principal component analysis and orthogonal partial least squares discriminant analysis showed consistent results， all of which could classify the 15 batches of samples into three categories. The linear range of indicator components in 15 batches of Pinghuo tea standard decoction， such as geniposide， luteolin， isochlorogenic acid A， glycyrrhizin， and glycyrrhizic acid， were 0.020 580-0.411 600， 0.001 617-0.080 850， 0.006 076-0.607 600， 0.005 125-0.071 740， and 0.017 288-0.432 200 mg/mL， respectively； RSDs of precision， repeatability， stability and recovery rate tests were all not higher than 4% （n＝6）. The mass fractions ranged 3.227 9-10.002 2， 0.297 4-0.554 6， 3.350 1-6.159 6， 0.720 6-1.073 3， 2.003 1-3.030 1 mg/g； transfer rates from the pieces and standard decoction were 19.762 8%-35.840 5%， 12.123 3%-21.254 0%， 46.097 2%-82.869 4%， 58.708 8%-91.629 6%， 39.114 3%-63.710 6%. The transfer rates of the extract from 15 batches of Pinghuo tea standard decoction ranged from 61.15%-84.68%. CONCLUSIONS Established HPLC fingerprint and content determination methods in this study are simple and accurate， which can provide reference for the quantitative value transfer study， quality control， clinical application and the development of subsequent formulations of Pinghuo tea standard decoction.