Connective tissue growth factor (CTGF) has recently received much attention as a possible key determinant of progressive fibrosis. It promotes tissue fibrosis through different pathways, such as cell proliferation, extracellular matrix accumulation and cell transdifferentiation. A number of regulators of CTGF expression have been identified, including transforming growth factor ?, vascular endothelial growth factor, tumor necrosis factor ?, etc. The mechanism of profibrotic effect by CTGF was reviewed. [

Objective To investigate the improvement role of aspirin combined with statins on carotid plaque of patients with cerebral thrombosis.Methods80 cases of patients with cerebral thrombosis in our hospital from May 2014 to May 2016 were selected, these patients were divided into aspirin combined with statins treatment group (combined treatment group, 40 cases) and single aspirin treatment group (single treatment group, 40 cases) two groups according to the treatment methods, the NIHSS scores, Barthel indexes, clinical curative effects, IMT, plaque areas of the two groups were statistically analyzed.ResultsThe NIHSS score of the combined treatment group was significantly lower (P<0.05), the Barthel index was significantly higher (P<0.05), the total treatment efficiency 95.0% (38/40) was significantly higher than the single treatment group 80.0% (32/40) (P<0.05), the IMT was significantly shorter (P<0.05), the plaque area was significantly smaller than the single treatment group (P<0.05).ConclusionAspirin combined with statins can more effectively improve the carotid plaque of patients with cerebral thrombosis than aspirin alone, so is worthy of promotion and use in the clinical.

Objective To investigate the relationship between hepatitis B virus (HBV) infection and hepatic metastasis from colorectal cancer.Methods Published literatures and unpublished conference papers from January 1990 to December 2011 were searched in PubMed,Cochrane library,Wanfang Database and CNKI.Data of the literatures were extracted by a table.All the patients with colorectal cancer were divided into the infected group and the control group according to the patients whether infected by HBV or not.The hepatic metastatic rate was analyzed.The Meta analysis was carried out by using the Review Manager 5.0 software,and the heterogeneity between studies was analyzed using the I2.Random effect regression model or fixed effects regression model was used according to the P value.The funnel plot was drawn to assess the potential for publication bias.The count data were presented by odds ratio (OR) and 95％ confidence intervals (95％ CI).Results Fifty-six articles were retrieved,and 11 of which met the criteria.The number of patients with colorectal cancer was 5622,and 924 were in the infected group,4698 in the control group.There was no statistical difference in the heterogeneity between all the articles (I2 =0,df=10,P ＞ 0.05).The hepatic metastatic rate was 10.61％ (98/924) in the infected group,which was significantly lower than 23.84％ (1120/4698) of the control group (OR =0.35,P ＜ 0.05).The funnel plot showed that there was no obvious publication bias in these studies.Conclusion Patients with colorectal cancer and infected by HBV have lower incidence of hepatic metastasis.

Objective In order to discuss the practicability and risk analysis of deep venous catheter in AIDS patients.Methods 112 patients who needed to keep the intravenous route between October in 2010 and February in 2012 in Yunnan AIDS care center were implemented deep venous catheter.Results Right Subclavian vein catheter 101 cases,right internal jugular vein catheter 10 cases,right femoral vein catheter in 1.Wound happened in 5 cases,catheter heterotopia 1 case.Conclusion Deep venous catheter in patients with HIV/AIDS clinical diagnosis and treatment work could not only relieve the patients' pain because of repeated puncture,reduce the nursing workload,ensure the smooth progress of the clinical diagnosis and treatment,but also the more important is to reduce the occupational exposure risk of medical staff.

Objective To estimate the biological effects of leptin on human HaCaT keratinocytes and explore their molecular mechanisms.Methods Cell counting kit-8 (CCK-8) was used to evaluate the proliferation of cultured HaCaT cells treated with different concentrations of leptin for 24 and 48 hours.Some HaCaT cells were classified into four groups to remain untreated,be treated with leptin (100 μg/L) and piceatannol (a specific inhibitor of STAT3 phosphorylation) alone or in combination for 24 hours,respectively,followed by the evaluation of cell proliferation using CCK-8 kit.Flow cytometry was performed to assess cell cycle of HaCaT cells treated with leptin of 100 μg/L,Western blot to determine the phosphorylation level of Erk1/2 and STAT3 in HaCaT cells treated with leptin of 100 μg/L for different durations.Statistical analysis was done by Student's t-test for unpaired data using GraphPad Prism 5 software.Results The proliferation of HaCaT cells was accelerated to different degrees after treatment with leptin of 50 and 100 μg/L for 24 and 48 hours,and the accelerating effect was in a dose-dependent manner within 24 hours (r =0.9989,P ＜ 0.05).Piceatannol apparently inhibited the promotive effect of leptin on the proliferation of HaCaT cells.There was an obvious elevation in the percentage of cells at S phase ((57.70 ± 5.88)％ vs.(42.50 ± 7.55)％,P ＞ 0.05),but a significant decrease in that at G0/G1 phase ((39.70 ± 1.57)％ vs.(45.20 ± 1.44)％,P ＜ 0.05),with a significant increase in proliferation index (0.603 ±0.0157 vs.0.564 ± 0.0144,P ＜ 0.05) in HaCaT cells treated with leptin of 100 μg/L for 24 hours compared with the untreated controls.Western blot showed that leptin of 100 μg/L markedly enhanced the phosphorylation level of STAT3 in HaCaT cells.Conclusion Leptin may upregulate the proliferation of HaCaT cells through activation of STAT3 pathway.

Objective To analyze Vitamin D nutritional status of children and their height growth relationship in Wanzhou dis-trict of Chongqing .Methods Electrochemiluminescence immunoassay method was used to detect serum 25 hydroxyvitamin D [25 (OH) D]concentrations in 2 727 Children from Wanzhou district of Chongqing ,analyzing the correlation of Vitamin D and children age ,gender ,height ,parental height and other factors .Results There was significant difference in serum level of 25(OH) D among different age groups(P<0 .01) ,there was significant difference between boys and girls among the same age groups of 5 months ,6 months ,4 years old ,5 years old .Children′s height consistented with the national average 4 -6 years ago in Wanzhou District ,but Since then ,gradually felled behind .After adjustment for age ,sex ,parental height ,measured seasonal and other factors ,serum 25 (OH) D concentration was positively correlated with height (r=0 .462 5 ,P<0 .01) .Conclusion 25(OH)D levels were closely re-lated with the development of children′s height .Thus ,children generally poor 25(OH)D nutritional status in Wanzhou district should arouse the attention of parents and clinicians .

Objective To investigate the effect of remote ischemic preconditioning on myocardial infarction and the involved protective mechanisms. Methods Twenty-four male SD rats were randomly divided into four groups: ischemia-reperfusion (I/R) group, rote ischemic preconditioning (RIPC) group, remote ischemic preconditioning + ischemia-reperfusion (RIPC+I/R) group, and RIPC+AG490+I/R group. The blood samples and myocardial specimens were collected and prepared for tests. The related enzymes were detected and the size of myocardial infarction was measured. The cardiac cells were determined by electron microscopy and light microscopy. Results The size of myocardial infarct and myocardial enzymes were significantly reduced in RIPC+I/R group compared to those in I/R group (P < 0.05). The size of myocardial infarction and myocardial enzymes were significantly increased in AG490 group compared to those in RIPC+I/R group (P < 0.05), but were significantly reduced in AG490 group compared to those in I/R group (P < 0.05). Conclusions Remote ischemic preconditioning may be effective in cardioprotection. The JAK/STAT pathway is involved in the cardioprotection of remote ischemic preconditioning.

OBJECTIVE:To provide reference for strengthening the management of drug off-label dosage in hospital. METH-ODS:PDCA cycle management was conducted to investigate the current situation of off-label dosage of Alprostadil injection (Li-po-PGE1)in endocrinology department of our hospital before Jul. 2012 in the Plan stage,identify problems,find reasons and devel-op intervention objectives and measures;after intervention in Do stage,medical advices were checked periodically in Check stage, and the data in the same stage of 2013 were spot-checked in Astage stage,intervention effect was analyzed and interventional pro-cess was standardized. RESULTS:Totally 943 medical advices,including 126 cases/times were analyzed before PDCA intervention and according to the data of cases,medical advices and drug usage amount,the incidences of off-label dosage were 39.68%, 31.50% and 47.90%,respectively;after a cycle of PDCA intervention,totally 414 medical advices,including 73 cases were ana-lyzed and the incidences of off-label dosage were 0. The prescribed daily dose(PDD)was decreased from 13.15 μg to the predeter-mined range(10.00 μg)with decrease rate of 23.95%;the drug use density(DUD)was decreased from 59.82 to 20.07 with de-crease rate of 66.45%. There were significant differences in among the incidence of off-label dosage,PDD and DUD of Lipo-PGE1 (P<0.05). It reached the expected targets. CONCLUSIONS:Because of its well-organized ideas on work and continuous improve-ment methods of circulation,PDCA cycle management provides a workable avenue to manage drug off-label dosage uses in hospital.

Aim To explore the antioxidation of pergolide on rat models of Parkinson disease and its mechanism. Methods Wistar rats were pretreated daily with pergolide(1.0 mg?kg -1,ip),pergolide combined with sulpiride(25 mg?kg -1) or sulpiride alone for 7 days, and then sacrificed by decapitation. The activity of SOD and GSH in striatum was determined. Unilateral Parkinsonian models were made in rest of the rats by stereotaxically injecting 6-OHDA. After two weeks, apomorphine (APO)-induced rotational behavior of each group was compared, and the concentrations of striatal dopamine (DA),dihydroxyphenyl acetic acid(DOPAC) and homovanilic acid(HVA) were determined by high performance liquid chromatography (HPLC). Result Administration of pergolide for 7 days significantly increased the act- ivities of SOD and GSH in the striatum (P