Objective To detect the effects of plasmin combined with hyaluronidase or hexafluoride SF6 on inducing posterior vitreous detachment (PVD). Methods Eighteen young pigmented rabbits were randomly divided into group A, B, and C with 6 rabbits in each. All of the right eyes were the experimental eyes and the left ones was the control. The right eyes in group A, B, and C were injected with plasmin 1 U, plasmin 1 U and hyaluronidase 20 U, and plasmin 1 U and SF_6 0.5 ml, respectively; while all of the left eyes underwent intra-vitreous injection with balanced salt solution 0.1 ml. The eyes were observed by indirect ophthalmoscopy, slit lamp examination, biomicroscopy, B-ultrasonography, and electroretinography (ERG) before and after injection respectively. At last, the retinal sections were examined by light microscopy, scanning and transmission electron microscopy. Results The results of scanning microscopy showed incomplete PVD in 2 (33.3%) experimental eyes in group A, and complete PVD in 4 (66.7%) experimental eyes in both group B and C, and the positive rate of PVD in both group B and C significantly differed from that in group A (P0.05). The results of transmission electron microscopy and light microscopy indicated unchanged retinal structure. Conclusions Compared with the application of only plasmin, plasmin combined with hyaluronidase or hexafluoride SF_6 can induce complete PVD more efficiently and do no harm to the retina.

Objective To observe the alteration of scavenger receptor class A types Ⅰand Ⅱ (SR-AⅠ/Ⅱ) gene knock-out on lipid metabolism in mice fed with high-fat diet, and explore the underlying mechanism. Method The SR-AⅠ/Ⅱgene knock-out and wild-type male mice were fed with normal and high-fat diet for 12 w. Thereafter, the level of lipid metabolism (such as the levels of lipids in blood and liver) was detected with enzyme method or oil red O staining, and the expression of scavenger receptor class B typeⅠ(SR-BⅠ) and CD36 in liver was analyzed by RT-PCR. Results Under high-fat diet condition, as compared with wild-type mice, the levels of TG, TC, LDL and HDL in SR-AⅠ/Ⅱgene knock-out mice were decreased at 3, 6, 12 w (P0.05). Conclusion The alteration of lipid metabolism induced by high-fat diet in SR-AⅠ/Ⅱgene knock-out mice might be relative with the up-regulated SR-BⅠmRNA expression and the counter transport of peripheral lipids to liver.

AIM: This study is to detail its possible mechanisms that homocysteine (Hcy) induces injury of cultured endothelial cells.METHODS: Hcy in sequential concentrations was added into the cultured human umbilial vein endothelial cells for 24 hours in serum-free medium. The lipid peroxidation, release of LDH, cell total protein content, cell apoptosis and necrosis were assessed. RESULTS: Hcy increased the apoptosis of endothelial cells.In high Hcy concentration the cells also showed obvious injurious and necrotic morphological changes. Lipid peroxidation increased, with LDH releasing up and cell total protein content down, and they showed a positive dose-effect relationship with the Hcy concentration. All the above effects of Hcy was strengthened by low density lipoprotein (LDL) which may suggest synergetic effects of Hcy and LDL.CONCLUSION: Our results indicate that Hcy has a strong oxidizing effect, which may be one of its major mechanism for injury of EC.

Objective In order to provide information for the health administration and the price authorities to establish the reasonable price,the practical cost of special nursing of the chronic wound be analyzed,and be compared with the present charge,and the deviation be studied.Methods The cost of nursing care for all items was measured,recorded and collected,then calculating the special nursing cost for chronic wound using the method of ladder sharing the project cost.Rusults The true cost on a single special care for chronic wound was (29.42±9.29)yuan,and it reached 2.5 times of present charge ( 12 yuan).Conclusions The present charge on the special care for chronic wound was seriously deviated from the practical cost,and the labor value of special care of nurses could not be embodied.

Objective To survey the status of clinical isolates multi-drug resistant Enterobacteriaceae produ-cing KPC carbapenemase in Huizhou city of Guangdong Province .Methods 81 strains of multi-drug resistant Enter-obacteriaceae were collectded from 2009 to 2011 in Huizhou region.Modified Hodge test(MHT) was used to screen stains which may produce carbapenemase , modified three-dimensional test was used to detect ESBLs , AmpC and MBLs.Polymerase chain reaction (PCR) and gene sequencing methods were performed to confirm KPC genotypes . Results Of 81 strains multi-drug resistant enterobacteriaceae ,13 of which had passitive results were screened by modified Hodge test ,including 7 strains of Enterobacter cloacae ,4 strains of Klebsiella pneumonia and 2 strains of Escherichia coli;By modified three-dimensional test ,9 strains produced MBLs ,2 strains produced ESBLs ,1 strain pro-duced AmpC ,1 strain produced ESBLs and AmpC at the same time .KPC was amplified from a strain of Klebsiella pneumonia which produce ESBLs by PCR ,KPC-2 genetype was identified by gene sequencing .Conclusion Klebsiel-la pneumonia with KPC–2 carbapenemase has emerged in huizhou city ,but the production of metallo-beta-lactamase is the main cause that Enterobacteriaceae resist to carbapenemase drugs .

Objective To investigate the clinical therapeutic effect of Xuebijing injection for treatment of patients with diabetic nephropathy(DN)and its mechanism. Methods 60 DN patients were randomly divided into Xuebijing group and control group(each,30 cases). The patients in both groups received western conventional treatment,and the patients in Xuebijing group received additionally Xuebijing injection intra-venous injection once a day for 14 days. The fasting blood glucose(FBG),glycosylated hemoglobin(HbA1c),urinary albumin excretion rate(AER),blood urea nitrogen(BUN),serum creatinine(SCr),hematocrit(HCT),fibrinogen(Fg),whole blood viscosity,total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C)and interleukin -6(IL-6),tumor necrosis factor-α(TNF-α)and urine β2-microglobulin(β2-MG)levels before and after treatment were detected,and the curative effect was also observed in both groups. Results In the control group blood FBG,BUN,SCr,TC,IL-6 and TNF-αafter treatment were significantly decreased and HDL-C significantly increased compared with those before treatment(all P<0.05). Compared with those before treatment,in Xuebijing group after Xuebijing therapy,blood FBG,β2-MG,AER,BUN, SCr,TC,TG,HCT,blood viscosity,IL-6 and TNF-αwere significantly decreased,and HDL-C was obviously increased,but there were no significant differences in HbA1c,LDL-C and Fg before and after treatment. The above indexes were changed significantly in Xuebijing group compared with those in control group〔FBG(μg/L):6.98±1.14 vs. 9.73±1.62,β2-MG(μg/L):32.1±10.9 vs. 57.2±15.1,AER(μg/min):86.0±28.1 vs. 152.0±51.6,BUN (mmol/L):12.4±8.1 vs. 19.5±8.9,SCr(μmol/L):301.2±151.9 vs. 371.3±168.6,HCT:0.283±0.075 vs. 0.351±0.059,TC(mmol/L):3.4±1.8 vs. 4.1±1.5,TG(mmol/L):3.4±1.5 vs. 3.6±1.7,HDL-C(mmol/L):1.90±0.75 vs. 1.50±0.25, IL-6 (ng/L):8.96±2.07 vs. 12.75±2.47, TNF-α(pmol/L):17.85±4.75 vs. 20.87±4.90,P<0.05 or P<0.01〕. The total efficiency in Xuebijing group was significantly higher than that in control group(83.3%vs. 36.7%,P<0.01). Conclusion Xuebijing injection has significant protective effects on patients with DN,and the mechanism might be associated with increasing tissue perfusion and inhibiting excessive inflammatory cytokines release.

Objective:To explore whether the Formula of Tonifying Kidney of Vital Essence can modulate secretion of IL-4 and IFN-? by the CD4+T cells from human decidua during first trimester,which will lead to further investigate the anti-abortional effect mechanisms of the Formula of Tonifying Kidney of Vital Essence.Methods:40 female adult SD rats were divided randomly into two groups:one group was gavaged with the Formula of Tonifying Kidney of Vital Essence;another was gavaged with normal saline,twice a day for 3 days.The two groups of rats were gavaged with the same volume of the decoction or saline.Thereafter the blood was collected from carotid artery.Human decidua tissues were collected by artificial abortion at 6-10 weeks of gestation.The CD4+ T cells were separated by MACS.CD4+ T cells were divided into four groups during cell culture.Group A was treated with 10% fetal calf serum,which was the control group.Group B was treated with 10% of the rat serum from the herbal medicine;Group C was treated with 20% of the rat serum from the herbal medicin.Group D was treated with the rat serum of the saline control.The supernatants were harvested at 24,48 and 72 h of the culture,respectively.The amounts of IL-4 and IFN-? in the culture supernatants were measured by ELISA.Results:In both group B and C of the culture the amount of IL-4 was evidently elevated when compared with the control,group A(P

OBJECTIVE: To study the lactagogue effect of Rutong granule on rat with postpartum hypogalactia. METHODS: Female rats which had kittening behavior within 24 h were selected to induce rat model by perfusing bromocriptinum. Meanwhile, model rats were given Rutong granule via i.g. gtt. Milk yield of female rats and body weight of young rats were determined everyday from the second day. Rats were sacrificed on the 8th day. The level of PRL and histological change of breast were observed. RESULTS: Rutong granule can increase the serum PRL level gradually to promote the increase of acinar number and dilatation of gland ducts. Rutong granules can also increase the milk yield of female rats and weight of young rats. CONCLUSION: Rutong granule can promote secretion of milk by means of increase of PRL level and expediting of secretion channels.

This study analyzed the current clinical terminology standardization, systematization research status of traditional Chinese medicine(TCM). The previous version of the TCM clinical term system had some problems including imperfect classification structure, unclear relationship between concepts and so on, which made the TCM clinical terminology system(TCMCTS) difficult to support the clinical practice of TCM. The suggestions for system improvement were using ontology method to build TCMCTS concept model bases on the previous researches and data extracted from TCM clinical electronic medical record, and top-level-ontology accordance with ISO standards. The study also tried to summarize the ‘semantic network between classes’ through semantic relationships.

Standards of TCM information plays a fundamental and critical role in TCM informatization. In recent years, the standardization of TCM information has made a series of tremendous progress. This article introduced the major work progress of TCM terminological standardization and TCM data standardization in China, and status of TCM information standardization in International Organization for Standardization and World Health Organization. The existing problems in the construction of standardization of TCM information were discussed and suggestions were proposed to solve problems: to build the basic framework of standard system of TCM information; to cultivate professionals for the researches on the international standards of TCM information; to further explore the theories and methods about standardization of TCM information.