Objective The molecular biology mechanism of cerebral ischemia animal models be analyzed from acupuncture experimental literatures by using data mining methods as a tentative exploration. Methods Retrieve the literatures of acupuncture animal experiments during 1978-2015, and screen the literatures about acupuncture effect on cerebral ischemia animal model of gene protein expression. Acupoint and gene/protein targets were extracted and normalized, and then the frequency statistics, association rule, calculation of complex networks were analyzed and visually displayed.Results A total of 350 papers were included. Seventy-nine acupoints were summarized, and the most frequent acupoint wasbaihui; A total of 180 genes/proteins were found, and the most frequent genes/proteins was BCL2. The most common group acuppointszusanli and quchifor the association rule results. The results of complex networks analysis showed the HSP70, BCL2, CASP3, Bax were more closely associated with cerebral ischemia injury or rehabilitation mechanism. Conclusions The mechanism of acupuncture treatment for cerebral ischemia was that acupuncture adjusting ischemic brain damage and improve the nervous system disorders from many channels and multiple targets.

Objective To evaluate the feasibility and clinical significance of magnetic resonance cholangiopancreatography (MRCP) in the diagnosis of anatomic variants of the extrahepatic biliary tract. Methods The MRCP images of 535 patients who were admitted to The Second Affiliated Hospital of Suzhou University from March 2008 to March 2009 were retrospectively analyzed by two reviewers independently. The anatomic variants of the extrahepatic biliary tract were evaluated by studying the T2-weighted magnetic resonance images. Results The consistency between the two reviewers in the diagnosis of low choledochal joint, medial cystic duct insertion, aberrant hepatic duct, accessory hepatic duct and other anatomic variants was 98% , 99% , 89% , 90% and 100%, respectively (K = 0.86, 0.93, 0.81, 0.82, 1.00). The extrahepatic biliary system was clearly displayed by MRCP in 500 patients, and anatomic variants were observed in 240 patients, including low choledochal joint in 85, parallel cystic duct in 37, medial cystic duct insertion in 82, aberrant hepatic duct in nine, accessory hepatic duct in 10, choledochal cyst in three and high cystic duct in seven. A total of 259 patients underwent operation, and 168 patients had anatomic variants of the extrahepatic biliary tract. Conclusion MRCP imaging enables the accurate assessment of anatomic variants of the extrahepatic biliary system and avoids bile duct injury.

The investigation of characteristic databases in 22 domestic colleges or universities of traditional Chinese medicine showed that the information resources have not been fully developed and the developed databases of tradi-tional Chinese medicine lacked of deep hierarchies.It was pointed out that updating their contents and deepening their hierarchies are the developing orientation for the characteristic databases of traditional Chinese medicine.

Objective To evaluate the correlation between low choledochal joint and common bile duct stones(CBDS)by magnetic resonance cholangiopancreatography(MRCP).Methods The clinical data of 129 patients without intrahepatic calculus after cholecystectomy receiving MRCP from March 2008 to August 2009 were retrospectively analyzed.MRCP images and T2WIs were analyzed independently by 2 doctors to classify anatomic variants of cystic duct and calculus.Results CBDS was found in 49 and low insertion of the cystic duct in 31 of the 129 patients(38% vs.24%).For the latter 31 patients, low insertion of the cystic duct with CBDS was found in 17.The analysis showed that low choledochal joint were associated with CBDS(x2 =4.921, P=0.027).Conclusion There is a certain correlation between low insertion of the cystic duct and CBDS.The low insertion of the cystic duct is one of the risk factors for calculus of the common bile duct.

Objective:To collect and analyze the quality control data of ventilator, so as to apply these statistical data to guide the maintenance and management of ventilator.Methods: The displayed value and monitoring value of the tidal volume, respiratory frequency, inspiratory oxygen concentration, inspiratory pressure level and positive end expira tory pressure (PEEP) under different setting of 50 using ventilators in hospital were collected by using PF-300 gas analyzer, and then each parameter was analyzed as classification.Results: The total quality rate of ventilators was 80%, the ranking of quality rate as different brand was: brand B>brand A>brand C, and it was as different department: department 5>department 4=department 3>department1>department2, and the quality rates of less than 3 years and more than 3 years were 87% and 77%, respectively, as service life of ventilator. And the ranking of quality rates as parameters of ventilator was: PEEP=respiratory frequency >peak pressure>tidal volume >oxygen concentration. It was as accuracy of monitoring: brand A>brand C>brand B. It was as stability of monitoring: brand A>brand B=brand C. On the other hand, it was as the accuracy of displayed value: brand A>brand B>brand C, while it was as the stability of displayed value: brand A>brand C>brand B.Conclusion: The analysis of quality control data only suits to the ventilator which is used in our hospital. The engineer can targeted to strengthen the ventilator maintenance work and improve the quality and safety of equipment, so as to give the better service for patients according to quality control data of using ventilators in hospital.

Objectives To observe the effects of levetiracetam (LEV) on spatial learning and memory ability in epileptic rats and the expression of synaptophysin (SYN) and growth-associated protein 43 (GAP-43) in the hippocampus of rats with epi-lepsy induced by lithium pilocarpine (Li-pilo). Methods SD rats were randomly divided into normal control group (normal saline group), LEV group (treatment group), Li-pilo group (model group) and LEV control group (normal dose group). Twenty-four hours after the establishment of model, LEV (150 mg/kg per day) was given to rats in LEV group and LEV control group, and equivalent normal saline was given to rats in normal control group and Li-pilo group. At 7, 14 and 28 days after successful model building, the expression levels of the SYN and GAP-43mRNA in hippocampus of rats in each group were detected by RT-PCR. In the 4th week, the ability of learning and memory of rats in each group was evaluated by the Morris water maze. Results There was no significant difference in escape latency and the frequency of crossing platform between LEV control group and normal control group in Morris water maze test. Compared with normal control group, the rats in Li-pilo group had a longer latency to escape and reduced frequency of crossing platform (P<0.05). Compared with Li-pilo group, the rats in LEV group had shorter latency to escape and more frequency of crossing platform (P<0.05). There was no significant difference in the expressions of SYN and GAP-43mRNA in hippocampus of rats between LEV control group and normal control group. The expressions of SYN and GAP-43mRNA in Li-pilo group were lower than those of normal control group and LEV group (P<0.05). Conclusions Levetiracetam may improve learning and memory ability of rats with epilepsy through the regulation of SYN and GAP-43mRNA expressions in hippocampal tissue.

Objective To explore the effect of early stage of comprehensive lung rehabilitation on cervical spinal cord injury in patients with mechanical ventilation.Methods The patients with cervical spinal cord injury admitted from January to December 2015 were selected as the control group which was treated with routine nursing measures.The patients with cervical spinal cord injury admitted from January to December 2016 were selected as the treatment group,which received early stage lung rehabilitation measures.We compared two groups of patients in the using time of breathing machine,the total length of hospital stay and pulmonary infection rate.Results The duration of mechanical ventilation and hospitalization days in the control group were(13.98±11.11),(25.10±16.98)d,which were higher than those in treatment group[(7.23±4.94),(16.26±6.41)d](P<0.05).The infection rates of control group and treatment group were 76.19％and 52.38％respectively,and the difference was significant(P<0.05).Conclusion In the early stages comprehensive pulmonary rehabilitation measures for mechanical ventilation in patients with cervical spinal injury can shorten the breathing machine use time and the total hospitalization days,and reduce pulmonary infection rate.

Objective To study the absorption mechanism of the antihypertensive peptide,Val-Leu-Pro-Pro-Val(VLPVP) in Caco-2 cell model. Method Caco-2 cell model was used in vitro to investigate the effects of time,pH,drug concentration,enhancers and inhibitors on the absorption of VLPVP. VLPVP concentration was measured by HPLC. Results The transport rate of VLPVP reached the maximal level at pH7.4,and was positively correlated to transport time and drug concentration. The peptide transporter inhibitors(Gly-Pro,arphamenine A) ,the endocytosis inhibitor(phenyl arsenoxide) didn’t influence the transport of VLPVP,while the paracellular transport enhancer(sodium deoxycholate) ,the inhibitor of multi-drug resistant protein(MK-571) and the energy inhibitor(sodium azide) enhanced it from apical to basolateral side significantly,but the latter two didn’t influence the transport from basolateral to apical side. Conclusion Paracellular diffusion was suggested to have been the main mechanism for the absorption of intact VLPVP and MRP2 had strong eflux effect on the transepithelial transport of VLPVP.

Objective To define the mutation spectra of deafness gene in 318 Chinese Han population with nonsyndromic hearing loss (NSHL). Methods From October, 2015 to April, 2016, anticoagulant venous whole blood of 318 patients with NSHL were collected. The genes including GJB2, SLC26A4, GJB3 and 12Sr RNA were detected with polymerase chain reaction (PCR) and Matrix Assisted Laser De-sorption/Ionization Time-Of-Fight Mass Spectrometry (MALDI-TOF MS). Results Among these patient, 111 cases (34.9%) had GJB2 muta-tions, in which the mutation carrying rate of 235delC was the highest (25.47%), 43 cases (13.5%) had SLC26A4 mutations, 3 cases (0.94%) had GJB3 mutations, and 12 cases (3.77%) had mitochondria 12Sr RNA mutations. Conclusion Definition of mutation spectrum among dif-ferent populations with NSHL is important for development of optimal genetic screening services for congenital hearing impairment.

Objective To investigate the trait coping styles, emotional intelligence, positive mental characters and the impact of event among Chinese recruits, and the relationship among them. Methods The Trait Coping Style Questionnaire (TCSQ), Schutte's Emotional Intelligence Scale (SSEIS), Positive Mental Characters Scale for Recruits (PMCS-R), and Impact of Event Scale-Revised (IES-R) were used to assess the coping styles, emotional intelligence, positive mental characters and the impact of event in 1 837 male recruits that were divided into two groups (stress group and control group) by considering the scores of the IES-R as the screening criteria (≥35). The data were analyzed by t test, correlation analysis and multiple stepwise regression analysis. Results Totally 148 (8.1%) male recruits with stress were screened, and 163 male recruits without stress were selected using stratified random sampling according to demographic characteristics of age, education level and so on. There were significant differences between stress and control groups in the scores of TCSQ, SSEIS and PMCS-R except utilization of emotions and wisdom. Multiple stepwise regression analyses showed that the negative coping, regulation of emotion and wisdom entered the regression equation of the total score of IES-R in the stress group (F=13.395, R2=0.218, P<0.05). However, in the control group, only negative coping came into the equation of the total score of IES-R (F=22.887, R2=0.155, P<0.05). Conclusion Trait coping styles, emotional intelligence and positive mental characters can predict the impact of stress event among recruits effectively, which could provide theoretical basis for the mental health service in recruits.