BACKGROUND: The effect and mechanism of peroxisome proliferator-activated receptor gamma (PPARγ) agonists on transforming growth factor (TGF)-β1-induced fibrotic responses in lung fibroblasts remain unclear, and seldom reported.OBJECTIVE: To study the effects of PPARγ agonists on TGF-β1-induced fibrotic responses in lung fibroblasts, so as to investigate its effects in preventing pulmonary interstitial fibrosis of silicosis. METHODS: The pulmonary alveolar macrophage was prepared with 200 mg/L SiO2 and the supernatants were got. Chinese hamster lung fibroblasts were incubated with the prepared supernatants. Then a cellular model in vitro for studying pulmonary fibrosis induced by SiO2 was developed. Chinese hamster lung fibroblasts were cultured to observe the effects of the PPARγ ligand (15 d-PGJ2) and its agonists (troglitazone and ciglitazone) on the expression of TGF-β1-induced fibronectin. RT-PCR was used to detect the mRNA expression of fibronectin and Western blotting was used to detect the protein expression of fibronectin. RESULTS AND CONCLUSION: (1) The expression of TGF-β1 mRNA showed an effect in a dose- (0-5 μg/L) and time- (0-24 hours) dependent manner. (2) PPARγ ligands and agonists reduced the mRNA and protein expressions of fibronectin. Results suggested that PPARγ agonists can inhibit TGF-β1-induced fibronectin synthesis after stimulation by SiO2 and may play a potential role in preventing pulmonary interstitial fibrosis.

ObjectiveTo observe the clinical efficacy of of mouse nerve growth factor and Safflower Flavin on elderly diabetic patients safflower flavin cerebral infarction. Methods 80 elderly diabetic patients with cerebral infarction were randomly divided into treatment group and control group.The control group was treated with oxygen, dehydrating agent,controlling blood sugar,maintaining electrolyte balance,aspirin and injecting safflower flavin. On the basis of control group,the treatment group was given rat nerve growth factor. Before and after treatment two weeks,clinical symptoms and neurological function of patients were estimated. Resuts There were statistical significant differences in the clinical symptoms and neurological function of patients before and after treatment. ConclusionIt is an effective treatment with mouse nerve growth factor and safflower flavin on elderly diabetic patients with cerebral infarction and it is worthy of spreading in the clinic.

NMDA receptor has shown to be involved in several central nervous system disorders such as stroke, pain, parkinson′s disease, Huntington′s disease and epilepsy. Early studies revealed that NMDA antagonists produced adverse side effect that hampered the effort to develop NMDA antagonists. With advances in understanding the structure of NMDA receptor, NR2B antagonist is expected to be a more effective candidate with little side effect to treat these diseases. Several NR2B antagonists including CP 101, 606 are in preclinical trials in foreign countries. The present review summarizes progress in pharmacological actions of NR2B antagonists on neurodegenerative diseases.

[ ABSTRACT] AIM:To compare the expression of SIRT2 in ovarian surface epithelial ( OSE) cell line and serous ovarian carcinoma ( SOC) cell lines, and to investigate the effects of SIRT2 on the cell proliferation, migration and inva-sion.METHODS:The expression levels of SIRT2 in the OSE cell line and the SOC cell lines were determined by Western blot.The SIRT2 siRNAs and overexpression construct were designed and verified.Transient transfection of SIRT2 siRNAs or overexpression construct was performed, and the effect of SIRT2 on the cell proliferation, migration and invasion was e-valuated.RESULTS:SIRT2 levels in the 5 strains of SOC cell lines were significantly lower than that in the OSE cell line.SIRT2 knockdown in HOSEpiC cells significantly enhanced the ability of cell colony formation and accelerated the cell growth rate.On the contrary, overexpression of SIRT2 in HO8910 cells dramatically repressed the number of cell colonies and cell activity.SIRT2 significantly changed the ability of ovarian cell migration.Knockdown of SIRT2 facilitated the cell invasion.CONCLUSION:The expression of SIRT2 in the SOC cells is significantly down-regulated.In the OSE cells, SIRT2 acts as a tumor suppressor and mediates the inhibition of cell proliferation, migration and invasion.

Objective To investigate the situation of implementation of community home endowment nursing services in Yancheng City to provide basis for developing nursing services for the elderly.Methods Survey was conducted for the 255 elderly in 15 aged-service stations.We used the multistage random sampling by questionnaire and interview.Results There was difference in the need of community home endowment service,the situation of implementation,and in the service evaluation.Conclusions Nursing service for old people in community home endowment is still developing in elementary step.We should establish the normative and exercisable quality specifications and evaluation systems of nursing service for old people,in order to assure quality of nursing service.

Objective To study the correlation between symptomatic, radiological and etiological classification in acute ischemic stroke. Methods One hundred and twenty five patients with ischemic stroke within 48 hour of onset were prospectively studied with three step diagnosis: (1) symptomatic classification based on the Oxfordshire Community Stroke Project criteria (OCSP), (2) radiological classification(CT or MRI) and (3) etiological classification based on the Lausanne Stroke Registry criteria.Results Most of the patients with symptoms of total anterior circulation infarcts (TACI), partial anterior circulation infarcts (PACI) and posterior circulation infarcts (POCI) according to OCSP classification had corresponding lesions on CT or MRI, while only 67.3% of lacunar infarcts (LACI) patients had small subcortical infarction (SSI). More than 60% of patients with TACI were classified into cardioembolism (CE) in the third diagnosis, while the etiology of PACI was either CE or large artery atherosclerosis (LAA) in equal numbers. Only 57.7% of LACI patients were classified into small artery disease (SAD) and 28.8% of them into LAA, of which 80% patients had lesions other than SSI. The positive predictive value of SAD in the combination of LACI and SSI was 0.78. The etiology of POCI was variable.Conclusion Except for LACI, the symptomatic classification by OCSP corresponds well to the radiological classification. The etiological classification can be predicted in TACI and PACI, but it is hard to make in POCI, a number of LACI are due to LAA.

OBJECTIVE To investigate the common pathogens and predisposing factors of postoperative pneumonia in patients with chest tumor.METHODS The data of 279 cases of postoperative pneumonia for open chest surgery were analyzed retrospectively.RESULTS The prevalence of postoperative pneumonia for open chest surgery were mainly due to Gram-negative bacteria(41.4%).Gram-positive bacteria and fungis weighed 32.6% and 26%.The risk factors of postoperative pneumonia in chest tumor were aged,severe underlying disease, use of broad-spectrum antibiotics,surgery trauma,mechanical ventilation and invasive treatments patients.CONCLUSIONS It is very important to enforce the preoperative comprehensive management of open chest operation in order to reduce the incidence and mortality of postoperative pneumonia in chest tumor.

Objective To study the apheresis procedure of collecting mononuclear cells from bone marrow for clinical treatment.Methods Bone marrow mononuclear cells were separated from bone marrow using COBE Spectra apheresis system.Cell morphology,cell counts,positive expression of CD34+,CD133+,CD271+ were detected,and the recovery rate of nucleated cell and mononuclear cell were also calculated.And the main parameters of the procedure with upper efficiency were set via data analysis.Results The efficiency of collection line with colourimetric card of 3% was higher than that of 5% and of 7%.The recovery rate of mononuclear cell is 75% when the cycle times ranged from 7 to 9.The positive expression of CD34+,CD133+ and CD271+ were 1.17%,0.63%,and 0.53%,respectively.Conclution This mononuclear apheresis procedure is safety,pollution-free and efficient,and remaming the bone marrow could be retransfused to the donor.

Objective To explore the association of expression of apoptosis-associated gene BAK and cFLIP with the biological behaviors in endometriosis. Methods The expression of BAK and cFLIP protein gene in eutopic and ectopic tissue samples from 40 cases with pathologically confirmed ovarian endometriosis and 40 cases with pathologically confirmed normal endometrium was detected by immunohistochemical method. Results ①The expression of BAK and cFLIP protein gene was found in three groups of different endometrial tissue. ②The expression of BAK protein gene was increased gradually in ectopic endometrial , eutopic endometrium and normal tissue and there was significantly difference between every two groups ,while cFLIP was contrary expressed (P <0.05); ③In normal endometrium, The expression of BAK protein gene in secretory phase was higher than that in proliferative phase (P < 0.05), while cFLIP was contrary expressed, in eutopic endometrium and ectopic endometrial tissue , The expression of BAK and cFLIP protein gene in secretive phase showed no statistically significant difierence with that in proliferative phase (P > 0.05). ④The expression of BAK protein gene in severe group (Ⅲ-Ⅳ period) is lower than mild group both in eutopic or ectopic endometrial tissues,while cFLIP was contrary expressed (P < 0.05). ⑤The expression of BAK and cFLIP was negatively correlated with each other in ectopic endometrium (r=-0.389,P< 0.05). Conclusion BAK and cFLIP was negatively expressed in EMS, which may take a part in the endometrial apoptosis and disorderly proliferation. BAK and cFLIP may play an important role in the the diagnosis and treatment of endometriosis.