Objective To investigate the diagnostic value of contrast-enhanced ultrasound endoscopy(CE-EUS) for the upper gastrointestinal tract and adjacent lesions.Methods One hundred and eighty samples of upper gastrointestinal tract and adjacent lesions in the First Affiliated Hospital of Guangxi Medical University from June 2014 to March 2015 were collected and performed CE-EUS.The preliminary diagnosis was taken by observing the features of various kinds of solid mass,which was conducted the contrastive analysis with the results of fine needle aspiration cytology(EUS-FNA) and (or) histopathology, biliary brush cytology smear results,operative pathologic findings,and CT,MRI scans and follow-up results.Results In 180 cases of gastrointestinal tract and adjacent placeholder,169 cases of CE-EUS were in line with the final results,while 11 cases were not consistent,and the diagnostic consistency rate was 93.9%.Conclusion CE-EUS can guide EUS-FNA puncture for increasing its positive rate..

Objective To investigate the application of 3D ultrashort TE double echo pulse sequence in the bone and joint MR imaging. Methods Eight volunteers and a porcine fibula in vitro with intact muscle were involved in this study. Among the volunteers, one was suspected with meniscus tear, the others were asypmtomatic. MR imaging of 3D ultrashort TE double echo pulse sequence were performed on the tibial diaphysis, knee joint, ankle and wrist of each volunteer and the porcine fibula in vitro. Using the first echo images subtract with the second echo images, we observed the subtracted images from the primary double echo images and MPR images respectively. We then compared the difference of SNR. Four different echo times of the first echo (TE1) in the images were set as 0. 08 ms, 0. 16 ms, 0. 24 ms, 0. 35 ms. The quality of the subtracted images from the primary double echo images of the four different TE1 was compared.The MIP images from the primary double echo images with TE1 of 0. 08ms were performed to display the 3D structure of the ankle tendons. The data were analysed with One-Way ANOVA and Paired-Samples t test statistically. Results The 3D images of the tendons were displayed through MIP of the subtracted images from the primary double echo images. The cortical bones, periosteums, tendons and menisci of the 8 volunteers appeared as high signal intensity in UTE pulse sequence. The SNR of the subtracted images from MPR images (SNR, 3.76 ± 0. 88) was significantly higher than those from the primary double echo images(SNR,2. 82±0. 75) (t = - 4. 851, P < 0. 01). There were significant differences among the subtracted images from each of the four different TE1. The highest quality were obtained from the TE1 of 0.08ms. The CNR were as follows: CNR<,0.08ms>1.74±0. 54, CNR<,0.16ms> 1.35 + 0. 60, CNR0.24ms>1.20±0. 48,CNR<0.35ms> 0.89±0. 24 (F = 3. 681, P < 0. 05). The artifacts appeared markedly with prolonging of the TE1.Conclusion The MRI of ultrashort TE double pulse sequence may display the short T2 components that appeared as low signal with conventional clinical MR imaging, which made it pessible to quantify the tissues containing a majority of short T2 components.

This study sought to make a biomechanical analysis of the diffuse axonal injury(DAI) animal model caused by nonimpact with half bound head in cats. A three-dimensional finite element model of cat's head was established. The head of an anesthetized cat was scanned in 2 mm section. The nods and element meshes were signed out according to the geometry of every section. The geometric data were put into the computer and the element mesh body of cat's head was established in vizi CAD system. The maximum stress, minimum stress and von Mises stress were calculated by Super SAP (93ed) finite elemental software when the force was loaded on the right or left side of model in zero section. The analysis showed that the maximum stress appeared in the anterior and posterior loaded point and extended to cranial base in the cranial shell. There was high stress in the brain surface also. Because of cerebellar tentorium, cerebral falx, petrosal bone and sellar process, the stress did not decrease equivalently while approaching the deep brain, but it was distributed in cerebral-cerebellar peduncles, brain stem, corpus callosum and basal ganglia area at high values. The results suggest that the stress caused by rotational force is widespreadly and unequivalently distributed in brain tissue, which is mainly effected by the cerebellar tentorium, cerebral falx and the irregular geometric forms of cranial bone.
Animals ; Biomechanical Phenomena ; Brain ; pathology ; Brain Injuries ; pathology ; Cats ; Diffuse Axonal Injury ; pathology ; Finite Element Analysis ; Head ; Models, Animal ; Rotation ; Skull

Objective:To compare the value of Liaoning scoring system, model for end-stage liver disease (MELD), model for end-stage liver disease-Na (MELD-Na) and Blatchford score in predicting high-risk esophageal varices (EVs), hemorrhage or re-hemorrhage within 1 year and blood transfusion treatment in cirrhotic patients.Methods:Clinical data of 170 patients with esophageal varices confirmed by endoscopy from January 2018 to September 2019 were recorded. Liaoning score, MELD, MELD-Na score and Blatchford score were calculated when the first endoscopy was performed. These patients were followed up, and hemorrhage or re-hemorrhage within 1 year was recorded. Receiver operating characteristic (ROC) curve was drawn and the area under curve (AUC) was used to evaluate the accuracy of 4 scoring systems in predicting high-risk EVs, hemorrhage or re-hemorrhage within 1 year after the first endoscopy and blood transfusion. Cut-off values were obtained, and groups divided by cut-off values were compared for the proportion of high-risk EVs and hemorrhage or re-hemorrhage.Results:The cut-off value of high-risk EVs in patients with cirrhosis predicted by Liaoning score was 0.45, and the AUC was 0.702 (95% CI:0.612-0.781, P<0.01), superior to MELD, MELD-Na and Blatchford score (AUC were 0.593, 0.648, 0.610, respectively). The proportion of high-risk EVs in Liaoning score ≥0.45 and <0.45 were 71.8% (89/124) and 34.8% (16/46) with significant differences ( χ2=19.442, P<0.01). The AUC of Liaoning score for predicting hemorrhage or re-hemorrhage within 1 year was 0.680 (95% CI: 0.595-0.765, P<0.01), superior to MELD, MELD-Na and Blatchford score (AUC were 0.605,0.615,0.598, respectively). AUC of Blatchford score for predicting blood transfusion was 0.775 (95% CI:0.687-0.863, P<0.01), superior to MELD, MELD-Na and Liaoning score (AUC were 0.653, 0.719, 0.631, respectively). Conclusion:Liaoning score can predict high-risk EVs, hemorrhage or re-hemorrhage within 1 year after the first endoscopy in patients with cirrhosis and is superior to MELD, MELD-Na and Blatchford score. Blatchford score can effectively predict whether cirrhosis patients with EVs need blood transfusion.

Bone remodeling and bone regeneration are essential for preserving skeletal integrity and maintaining mineral homeostasis.T cells,as key members of adaptive immunity,play a pivotal role in bone remodeling and bone regeneration by producing a range of cytokines and growth factors.In the physiological state,T cells are involved in the maintenance of bone homeostasis through interactions with mesenchymal stem cells,osteoblasts,and osteoclasts.In pathological states,T cells participate in the pathological process of different types of osteoporosis through interaction with estrogen,glucocorticoids,and parathyroid hormone.During fracture healing for post-injury repair,T cells play different roles during the inflammatory hematoma phase,the bone callus formation phase and the bone remodeling phase.Targeting T cells thus emerges as a potential strategy for regulating bone homeostasis.This article reviews the research progress on related mechanisms of T cells immunity involved in bone remodeling and bone regeneration,with a view to providing a scientific basis for targeting T cells to regulate bone remodeling and bone regeneration.