Objective:To investigate the effectiveness and safety of middle-column preserved pedicle subtraction closing-opening wedge osteotomy for the treatment of stiff kyphosis.Methods:From January 2016 to April 2018, 12 patients with stiff kyphosis in our department were treated with middle-column preserved pedicle subtraction closing-opening wedge osteotomy. The patients' operative time, intraoperative blood loss, postoperative drainage, surgical complications, low back pain and leg pain visual analogue scale (VAS), Oswestry dysfunction index (ODI) score, and SF-36 were recorded.These parameters were compared at preoperative, postoperative, and at the final follow-up. Coronal parameters included lumbar scoliosis Cobb angle, C 7 vertebral body center to humeral vertical line distance (C 7PL-CSVL), whilesagittal parameters includedlumbar Lordosis (LL), sacral slope (SS), pelvic tilt (PT), and sagittalvertical axis (SVA). Results:All of 12 patients successfully completed the operation.The mean operation time was 238.20±65.95 min, the mean intraoperative blood loss was 440.50±133.60 ml.The patients’ODI score was 65.92%±6.96% at the preoperative, and 21.00%±3.19% at the final follow-up. The difference was statistically significant ( t=20.32, P<0.0001).The VAS score of back pain was 6.00±0.95 at preoperative, 2.33±0.89 at 3 months postoperatively, and 1.42±0.51 at the final follow-up. The VAS score of leg pain was 6.91±1.24 at preoperative, 2.50±1.00 at 3 months postoperatively, and1.50±0.52 at the final follow-up. There was significant difference in SF-36 at preoperative and at final follow-up ( P<0.05). The differences in LL, SS, PT and SVA at the preoperative and at final follow-up were statistically significant ( F=17.47, P<0.001; F=5.015, P=0.0125; F=14.66, P<0.001; F=81.11, P<0.001) . There was significant difference in lumbar scoliosis Cobb angle and C 7PL-CSVL at the preoperative and at final follow-up ( F=87.19, P<0.001; F=100.9, P<0.001) . Conclusion:The advantages of this surgical procedure includesimple operation, reducedsurgery time, and shorten intraoperative bleeding, which can effectively relief clinical symptoms, improve the quality of life, correctkyphosis, and maintain the patient's spinal-pelvic balance.

Changes in nuclear morphology are common in malignant tumors, but the underlying molecular mechanisms remain poorly understood. Lamins is involved in supporting nuclear structure, and the expression of Lamins is the molecular basis for nuclear morphological changes during tumor progression. In recent years, the research on the relationship between Lamins and malignant tumors has made great progress. Lamins is of great value in the diagnosis, treatment, and prognosis of various malignant tumors.
Cell Nucleus ; Humans ; Lamins/genetics* ; Neoplasms/genetics* ; Prognosis

ObjectiveTo investigate primary and secondary transfer of exfoliated cells from human hands on non-porous substrates such as plastic steering wheel or computer mouse. MethodsDNA detection sensitivity and detection limit for mixed DNA profiling were examined to understand our laboratory’s ability to test for trace DNA. Forensic swabs were used to collect samples from volunteers’ one-hour-long unwashed hands， substrates touched by volunteers’ dominant hand 30 min after hand washing， substrates touched by volunteers 30 min after washing their hands and then immediately or 30 min following shaking hands， and individual A’s daily-use substrates touched by individual B and then by individual A again. Simulations were conducted to assess the potential for introduction of another person’s exfoliated cells from hands into routine casework samples. ResultsOur laboratory can obtain a full DNA profile from as little as 0.020 ng of DNA and detect minor components in a 1：9 mixed DNA sample. 85% of samples from unwashed hands yielded a full DNA profile. Primary transfer of a full DNA profile was found in 77% of substrates touched by volunteers’ dominant hand 30 min after hand washing， allowing differentiation between good and poor shedders， with no significant difference in genders and substrate types. 75% of substrates touched 30 min after hand washing and then immediately following handshaking yielded the other individual’s DNA profile （secondary transfer）， with the number of short tandem repeat （STR） loci detected ranging from 0 to 23； the percentage and number decreased substantially when the substrates were touched 30 minutes later. No foreign DNA was detected in routine casework samples with introduced exfoliated cells from hands. When two individuals took turns touching items with their hands， the major contributor to the DNA profile was not always the individual who made the last contact. ConclusionsPrimary and secondary DNA transfer can be detected on non-porous substrates， and based on the deposit of hand exfoliated cells， individuals can be categorized as good or poor shedders， which is an important factor affecting detection of DNA transfer. Besides considering the laboratory’s DNA detection sensitivity， if DNA is detected on substrates by hand contact， we need to take into account the potential for secondary transfer at different levels of activity when interpreting the results.