1.Mathematical cognitive function in children with sleep disordered breathing based on event-related potential
Liuwei SHI ; Chaoqun WANG ; Haitian MEI ; Fangqiao ZHAO ; Yang YI
Chinese Journal of Behavioral Medicine and Brain Science 2023;32(9):822-827
Objective:To study the characteristics of mathematical cognitive function in children with sleep-disordered breathing(SDB) by event-related potential (ERP).Methods:From October 2020 to October 2022, totally 22 cases of SDB children and 22 cases of normal children aged 8-11 were selected.All subjects performed mathematic tasks including calculating and deciding.The EEG changes and behavioral data of children with SDB and normal children were recorded.The latency and amplitude of N1, P2, N2, P3 in leads Fz were measured and compared by Matlab. SPSS 23.0 software was used for statistical analysis, and t test or Mann Whitney U test were used for two independent sample data. Results:(1)Behavior test: the interaction effect between group and type, the group main effect, and the type main effect in accuracy between SDB group and normal group were not significant ( F=0.470, 3.590, 0.003, all P>0.05). The group main effect and interaction effect between group and type in reaction time between SDB group and normal group were not significant ( F=0.465, 1.991, both P>0.05), while the type main effect was significant ( F=18.010, P<0.05). (2)ERP test: the N2, P3 latencies for addition in children with SDB were longer than those in normal group(N2: (371.38±34.23)ms vs (348.12±26.34)ms; P3: (610.72±64.78)ms vs (529.05±30.25)ms)( t=2.526, 5.358, both P<0.05). There was no significant difference between SDB group and normal group in ERP latency and amplitude for subtraction(both P>0.05). The N2, P3 latencies for multiplication in children with SDB were longer than those in normal group(N2: (439.20±24.28)ms vs (351.14±25.26)ms; P3: (531.71±35.42)ms vs (415.23±19.01)ms)( t=11.792, 13.590, both P<0.05). The P3 amplitudes in children with SDB was higher than that in normal group(P3: (3.16±4.78)μV vs (0.38±3.27)μV)( t=2.248, P<0.05). The P3 latency for correct judgment in children with SDB was longer than that in normal group(P3: (420.38±34.79)ms vs (398.54±33.71)ms)( t=2.115, P<0.05). The P3 latency for wrong judgment in children with SDB was longer than that in normal group(P3: (475.25±51.11)ms vs (414.88±27.53)ms)( t=4.878, P<0.05). Conclusion:The latency of N2 and P3 in ERP of SDB children is prolonged, and P3 latency is more sensitive than N2, indicating that SDB children have impairment of mathematical cognitive function.The latency changes of N2 and P3 occurs earlier than the behavioral changes (reaction time and accuracy), which can be used as one of the electrophysiological indicators for early assessment of mathematical cognitive impairment in SDB children.
2.Human umbilical cord mesenchymal stem cell-derived extracellular vesicles enhance the regenerative capability of fibrotic liver
Yunguo LEI ; Jia YAO ; Jun ZHENG ; Tongyu LU ; Jiebin ZHANG ; Jiaqi XIAO ; Yasong LIU ; Haitian CHEN ; Xuegang ZHAO ; Xingye YANG
Organ Transplantation 2023;14(3):379-
Objective To investigate the role of human umbilical cord mesenchymal stem cell-derived extracellular vesicle (hUC-MSC-EV) in the regeneration of fibrotic liver. Methods C57BL/6 mice were randomly divided into the 70% normal liver resection group (Oil+PHx group), 70% liver fibrosis resection group (CCl4+PHx group) and 70% liver fibrosis resection+mesenchymal stem cell-derived extracellular vesicle (MSC-EV) treatment group (CCl4+PHx+MSC-EV group), with 8 mice in each group. LX-2 cell lines were assigned into the phosphate buffer solution (PBS) group, transforming growth factor (TGF)-β group and TGF-β+MSC-EV group. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in mice after partial liver resection were detected in each group. The expression levels of liver fibrosis and proliferation-related parameters were analyzed in each group. The messenger RNA (mRNA) expression levels of epidermal growth factor (EGF), fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in LX-2 cells were detected in each group, and their effects on HGF expression in mouse liver were observed. Results Compared with the Oil+PHx group, the serum levels of AST, ALT and LDH were up-regulated, and the degree of fibrosis was more severe, the positive area of Sirius red and α-smooth muscle actin (α-SMA) staining was larger, and the expression level of α-SMA protein was up-regulated in the CCl4+PHx group. Compared with the CCl4+PHx group, the serum levels of AST, ALT and LDH were decreased, the degree of fibrosis was slighter, the positive area of Sirius red and α-SMA staining was decreased, and the expression level of α-SMA protein was down-regulated in the CCl4+PHx+MSC-EV group, and the differences were statistically significant (all
3.Self-assembled small molecule natural product gel for drug delivery: a breakthrough in new application of small molecule natural products.
Kangkang ZHI ; Jiacheng WANG ; Haitian ZHAO ; Xin YANG
Acta Pharmaceutica Sinica B 2020;10(5):913-927
Natural products, as a gift of nature to humanity, have long been used as drugs or pharmacological actives to help people cure various diseases. Yet we still know comparatively little about their ability to be materials. In recent years, some small molecule natural products isolated from traditional Chinese medicines have been found to have new features, namely, self-assembly to form gels (., natural product gels, NPG). However, the application development of these natural products is seriously lacking, which greatly weakens their practical value and delays the maturity of the field. Here, a series of self-assembled triterpenoid natural products are used as materials (gel scaffolds) to construct drug delivery systems. Surprisingly, these NPG not only exhibit the excellent self-healing, controlled gelation, good safety and sustained release, but also achieve synergistic treatment of tumors through bioactive natural products. Compared with non-bioactive gel scaffolds, NPG scaffolds show great advantages in tumor therapy, including optimal tumor inhibition, preferable health, better body recovery, stronger immune function, less toxic side effects and longer survival. The successful construction of NPG scaffolds not only takes full advantage of the self-assembled natural products, but also takes an important step in the development of new applications for natural products.
4.Photosensitive pro-drug nanoassemblies harboring a chemotherapeutic dormancy function potentiates cancer immunotherapy.
Jianjun CHENG ; Haitian ZHAO ; Bin LI ; Hua ZHANG ; Qianyu ZHAO ; Shiyao FU ; Ying HAN ; Weihong LU ; Jiahua SHI ; Xin YANG
Acta Pharmaceutica Sinica B 2023;13(2):879-896
Immunotherapy combined with effective therapeutics such as chemotherapy and photodynamic therapy have been shown to be a successful strategy to activate anti-tumor immune responses for improved anticancer treatment. However, developing multifunctional biodegradable, biocompatible, low-toxic but highly efficient, and clinically available transformed nano-immunostimulants remains a challenge and is in great demand. Herein, we report and design of a novel carrier-free photo-chemotherapeutic nano-prodrug COS-BA/Ce6 NPs by combining three multifunctional components-a self-assembled natural small molecule betulinic acid (BA), a water-soluble chitosan oligosaccharide (COS), and a low toxic photosensitizer chlorin e6 (Ce6)-to augment the antitumor efficacy of the immune adjuvant anti-PD-L1-mediated cancer immunotherapy. We show that the designed nanodrugs harbored a smart and distinctive "dormancy" characteristic in chemotherapeutic effect with desired lower cytotoxicity, and multiple favorable therapeutic features including improved 1O2 generation induced by the reduced energy gap of Ce6, pH-responsiveness, good biodegradability, and biocompatibility, ensuring a highly efficient, synergistic photochemotherapy. Moreover, when combined with anti-PD-L1 therapy, both nano-coassembly based chemotherapy and chemotherapy/photodynamic therapy (PDT) could effectively activate antitumor immunity when treating primary or distant tumors, opening up potentially attractive possibilities for clinical immunotherapy.
5.Epidemiological characteristics of Middle East Respiratory Syndrome outbreak in the Republic of Korea, 2015.
Nijuan XIANG ; Dan LIN ; Guangxu AN ; Haitian SUI ; Zhiyong YANG ; Dan LI ; Jian ZHAO ; Tao MA ; Yali WANG ; Ruiqi REN ; Xinyan ZHANG ; Daxin NI ; Yanping ZHANG ; Qun LI ; Email: LIQUN@CHINACDC.CN.
Chinese Journal of Epidemiology 2015;36(8):836-841
OBJECTIVETo analyze the epidemiological characteristics of Middle East Respiratory Syndrome (MERS) outbreak in the Republic of Korea in 2015 and provide related information for the public health professionals in China.
METHODSThe incidence data of MERS were collected from the websites of the Korean government, WHO and authoritative media in Korea for this epidemiological analysis.
RESULTSBetween May 20 and July 13, 2015, a total of 186 confirmed MERS cases (1 index case, 29 secondary cases, 125 third generation cases, 25 fourth generation cases and 6 cases without clear generation data), including 36 deaths (case fatality rate: 19%), were reported in Korea. All cases were associated with nosocomial transmission except the index case and two possible family infections. Sixteen hospitals in 11 districts in 5 provinces/municipalities in Korea reported confirmed MERS cases, involving 39 medical professionals or staff. For the confirmed cases and death cases, the median ages were 55 years and 70 years respectively, and the cases and deaths in males accounted for 60% and 67% respectively. Up to 78% of the deaths were with underlying medical conditions. Besides the index case, other 12 patients were reported to cause secondary cases, in which 1 caused 84 infections. One imported MERS case from Korea was confirmed in China on May 29, no secondary cases occurred. The viruses strains isolated from the cases in Korea and the imported case in China show no significant variation compared with the strains isolated in the Middle East.
CONCLUSIONThe epidemiological pattern of the MERS outbreak in Korea was similar to MERS outbreaks occurred in the Middle East.
Aged ; Coronavirus Infections ; epidemiology ; Cross Infection ; Disease Outbreaks ; Female ; Hospitals ; Humans ; Male ; Middle Aged ; Middle East Respiratory Syndrome Coronavirus ; Public Health ; Republic of Korea ; epidemiology
6.Evaluating the importation of yellow fever cases into China in 2016 and strategies used to prevent and control the spread of the disease
Chao Li ; Dan Li ; Shirley JoAnn Smart ; Lei Zhou ; Peng Yang ; Jianming ou ; Yi He ; Ruiqi Ren ; Tao Ma ; Nijuan Xiang ; Haitian Sui ; Yali Wang ; Jian Zhao ; Chaonan Wang ; Yeping Wag ; Daxin Ni ; Isaac Chun-Hai Fung ; Dexin Li ; Yangmu Huang ; Qun Li
Western Pacific Surveillance and Response 2020;11(2):5-10
Abstract
During the yellow fever epidemic in Angola in 2016, cases of yellow fever were reported in China for the first time. The
11 cases, all Chinese nationals returning from Angola, were identified in March and April 2016, one to two weeks after
the peak of the Angolan epidemic. One patient died; the other 10 cases recovered after treatment. This paper reviews the
epidemiological characteristics of the 11 yellow fever cases imported into China. It examines case detection and disease
control and surveillance, and presents recommendations for further action to prevent additional importation of yellow fever
into China.
7.Epidemiological characteristics of human avian influenza A (H7N9) virus infection in China.
Ruiqi REN ; Lei ZHOU ; Nijuan XIANG ; Bo LIU ; Jian ZHAO ; Xingyi GENG ; Yali WANG ; Chao LI ; Yong LYU ; Fuqiang YANG ; Ming YANG ; Haitian SUI ; Xu HUANG ; Ling MENG ; Zhiheng HONG ; Wenxiao TU ; Yang CAO ; Leilei LI ; Fan DING ; Zhe WANG ; Rui WANG ; Jianyi YAO ; Yongjun GAO ; Lianmei JIN ; Yanping ZHANG ; Daxin NI ; Qun LI ; Zijian FENG
Chinese Journal of Epidemiology 2014;35(12):1362-1365
OBJECTIVETo investigate the epidemiological characteristics of human infections with avian influenza A (H7N9) in China and to provide scientific evidence for the adjustment of preventive strategy and control measures.
METHODSDemographic and epidemiologic information on human cases were collected from both reported data of field epidemiological investigation and the reporting system for infectious diseases.
RESULTSA total of 433 cases including 163 deaths were reported in mainland China before June 4, 2014. Two obvious epidemic peaks were noticed, in March to April, 2013 and January to February, 2014. Confirmed cases emerged in 14 areas of China. Five provinces, including Zhejiang, Guangdong, Jiangsu, Shanghai, and Hunan, reported about 85% of the total cases. Median age of the confirmed cases was 58 years (range, 1-91), with 70% as males. Of the 418 cases with available data, 87% had ever exposed to live poultry or contaminated environments. 14 clusters were identified but human to human transmission could not be ruled out in 9 clusters.
CONCLUSIONHuman infections with avian influenza A (H7N9) virus showed the characteristics of obvious seasonal distribution, with certain regional clusters. The majority of confirmed cases were among the elderly, with more males seen than the females. Data showed that main source of infection was live poultry and the live poultry market had played a significant role in the transmission of the virus.
Adaptation, Psychological ; Aged ; Animals ; China ; epidemiology ; Demography ; Environmental Pollution ; Female ; Humans ; Influenza A Virus, H7N9 Subtype ; Influenza, Human ; epidemiology ; prevention & control ; transmission ; Male ; Meat ; Poultry ; Research Design