Objective To investigate the effect of death associated protein kinase(DAPK) carboxylic terminal peptide(DCTP) on inhibition of TNF-? to human embryonic lung fibroblast proliferation.Methods Eukaryotic Expression vector pcDNA3.1(+)-DCTP was constructed and transferred into human embryonic lung fibroblast in vitro.The expression of DCTP was detected by RT-PCR and Western blotting.The proliferation of human embryonic lung fibroblast was examined with MTT assay.Results Eukaryotic Expression vector pcDNA3.1(+)-DCTP expressed DCTP in human embryonic lung fibroblast.pcDNA3.1(+)-DCTP did not affect the proliferation of human embryonic lung fibroblast.However,DCTP inhibited the toxicity of TNF-?.Conclusion The toxicity of TNF-? is blocked by DCTP.It offers theoretical and technical guidance to the clinical study of DCTP for protecting normal cells from damage by TNF-? therapy.

In the field of colorectal cancer molecular biology,there are a number of biomarkers of prognosis and curative effect,including orotate phosphoribosyltransferase,P53,thymidylate synthase,glutathione S-transferase,methylenetetrahydrofolate reductase,dihydropyfimidine dehydrogenase,uridine-diphosphoglucuronosyl transferase,which can select the effective patients or the patients who can't bear the side effects and contribute to the individualized treatment of colorectal cancer.

Objective To analyze the prevalence of lower urinary tract dysfunction in stroke patients in the convalescent stage,its risk factors and the characteristics of urodynamics.Methods One hundred and thirty-one stroke patients in the convalescent stage were included in this study.Their general information,neurological system reviewing and past history were obtained,especially detailed micturitional histories.In patients with urinary symptoms,diagnosis and classification were made and relevant analysis and urodynamic study were performed.Results The prevalence of lower urinary tract dysfunction is 44.3%.The most common symptom was urinary incontinence(frequency,urgency and nocturia)followed by hesitancy,straining and urinary retention.The incidence of micturitional disturbance is higher 1.8 times in cognitive disfunction group than normal cognitive function group(χ~2=18.683,P=0.000);it was higher 1.2 times in patients with modified Bathel index scale≤20 than those with≥25(χ~2=16.832,P=0.000).Logistic multivariate analyses suggested that age(OR=1.622,95%CI 1.183-2.224),the during of urethral catheterization(OR=3.236,95%CI 1.335-7.840)and single or multiple lesions (OR=1.296.95%CI 1.083-1.550)to be the risk factors of lower urinary tract dysfunction at the convalescent stage in stroke patients.The urodynamics study found detrusor over activity in 78.5% patients,normal in 10.5%and detrusor areflexia in 10.5% patients.There was no detrusor-sphincter dyssynergia.Conclusions Lower urinary tract dysfunction is common after stroke with the storage symptoms to be the most common.Patients with lower urinary tract dysfunction show impaired cognitive function and poor ability in daily life.Age,during of urethral catheterization and single or multiple lesions are among risk factors of lower urinary tract dysfunction.Detruser overactivity is the most common finding in urodynamic study.Because the clinic symptoms are not often in accordance with the findings in urodynamics study,further urodynamics study are needed to benefit the treatment.

Objective To observe how geniposide as an anti-inflammatory agent through inhibition Toll-like receptor 7/nuclear factor-κB signaling pathways activation, as well as TNF-α and IL-6 release infectioned by influenza virus. Methods Epithelial cells was exposed to human influenza viruses A/Gui/81/23(H3N2) infection for 2 h before treatment with geniposide for 24 h. NF-κB responsive element luciferase reportor gene was transfected and dual luciferase cis-reporting systems was used to assay the transcriptional activity of NF-κB under the stimulated circumstance of influenza virus infection. The phosphory level and nuclear transposition of NF-κB was observed by fluorescence inverted microscope. RT-PCR was used to detect the gene transcription level of TLR7, TNF-α and IL-6. Results The relative luciferase reporter assay of NF-κB was apparently improved by influenza virus infection. But geniposide significantly repressed the relative value of luciferase. The phosphorylation level and rate for nuclear transposition of NF-κB was apparently improved by influenza A virus infection observed by fluorescence inverted microscope. But geniposide significantly repressed the phosphorylation level and rate for nuclear transposition. RT-PCR showed upregulation of TLR7 and pre-inflammatory markers TNF-α and IL-6 in A549 cells infected by influenza virus, geniposide had a significant effect on the expression of TLR7 and inflammatory markers TNF-α and IL-6 after treated with influenza virus. Conclusion Geniposide as an antiinflammatory agent antagonized influenza A virus infection through inhibiting Toll-like receptor 7/nuclear factor-κB signaling pathways activation, as well as on the downregulation of the downstream inflammatory markers target gene expression TNF-α and IL-6.

Objective To investigate the effects of mindfulness behavior training on emotional disorder and immunological function in elderly patients after hip fracture fixation surgery.Methods Sixty elderly patients with fixed hip fracture by operation were randomly divided into a training group and a control group with 30 cases in each group.The training group received mindfulness behavior training in addition to routine rehabilitation training,while the control group only received routine rehabilitation training.The mindfulness behavior training was administered 90 min for once a week,lasting for 5 weeks.The emotional disorder was assessed with the profile of mood states (POMS),and the immune system function were evaluated by the number of CD3 + 、CD4 + 、CD8 + in T cell subgroup before and after 5 weeks of training.Results Before training,there was no significant difference in scores of POMS and the cells number of CD3 +,CD4 +,CD8 + (P ＞ 0.05) between the two groups.After treatment,scores of POMS including tension-anxiety (13.14 ± 3.36 vs 15.90 ± 4.28),depression-dejection (18.10 ± 6.24 vs 20.06 ± 5.42),fatigue-inertia (16.53 ± 3.50 vs 18.98 ± 4.88),vgor-activity (26.68 ± 5.22 vs 22.43 ± 5.20) (P ＜ 0.05),the level of mindfulness practice(58.00 ± 4.78 vs 54.00 ± 4.89) and the cells number of CD3 +,CD4 +,CD8 + (75.75 ± 5.40vs 69.91 ± 4.42 ;39.54 ± 3.29 vs 34.44 ± 4.21 ; 39.82 ± 3.55 vs 36.82 ± 3.55) in experimental group improved more than those in control group (P ＜ 0.05).Conclusion Mindfulness behavior training can improve the patients' mood state and have positive promotion on immunological function.

Measurements were made from different landmarks of the superior and inferior alveolar arches in 197 skulls and in 243 mandibles. The data were divided into four groups: 2-5 years old(only deciduous teeth in occlusion); 6-12 years old(first permanent molars in occlusion, but not second molars); 12-17 years old (second permanent molars in occlusion, but not third molars); adults (third molars in occlusion, not including the aged). The results from the observation of the adult specimens are as follows:The indices of alveolar arches are about 118.2 in maxilla; 77.6 in mandible.The average distances from Prosthion (incision in mandible) to Canine point are 19.6mm (left side), 19.8mm (right side) in maxilla; 13.8mm (left side), 14.0mm (right side) in mandible.The distances from canine point to ectomolare (the lateral most margin of the al veolar cavities of second molars in maxilla; of third molars in mandible) are about 33.7mm (left and right sides) in maxilla; 43.7mm (left side), 44.5mm (right side). in mandible.The convergence distance of alveolar arches is 42.9mm in maxilla; 26.4mm in mandible and the convergence angle is 51.5? in mandible; 43.1? in maxilla.It was found that most of the superior and inferior alveolar arches were asymmetrical, the percentages of asymmetry being 82.2 in maxilla and 67.0 in mandible of adults.

Objective To investigate the effects of mindfulness behavior training on mood states and quality of life in old patients post fixation for hip fracture. Methods Patients were randomly divided into experiment group (n=30) and control group (n=30). The control group received routine rehabilitation, while the experiment group received mindfulness practice therapy in addition. They were evaluated with Profile of Mood States (POMS), Short Form of Health Survey (SF-36), and Mindful Attention Awareness Scale (MAAS) before and 5 weeks after treatment. Results There was no difference in the scores of POMS, MAAS and SF-36 before treatment (P>0.05). The scores of MAAS and SF-36, the subscores of tension-anxiety, depression-dejection, fatigue-inertia and vigor-activity of POMS improved more in the experiment group than in the control group (P<0.05). Conclusion Mindfulness behavior training can improve the mood state and quality of life of old patients post fixation for hip fracture.

As a kind of abnormal phenomena,mirror movement occurred after both stroke and Parkinson's disease,and influence the rehabilitation prognosis was uncertain.Recently,bilateral training has been used in the stroke patients and showed some effect.

In order to study the cause why the patients with psoriasis are susceptible to complicate with cardiovascular diseases, we have determined the levels of serum apolipoprotein A I, B 100, cholesterol and triglyceride in 30 cases of patients with psoriasis vulgaris by immunoquantitative, enzyme and triazole methods. The results showed that in comparison with the control group the levels of apolipoprotein A I and triglyceride were normal, the levels of apolipoprotein B 100 and cholesterol increased (both P

Objective To observe the effects of Sanxiaoyuchuang ointment on the mice inflammatory model induced by Dimethylbenzene,bacteria strains in vitro and mice pneumococcus pneumonia model.Methods Mice inflammatory model induced by Dimethylbenzene,in vitro bacteria strains model and mice pneumococcus pneumonia model were founded respectively,randomed and controlled methods were used,exposed to different dosages of Sanxiaoyuchuang ointment,the changes of auricle swelling degree,earcapillary vessel permeability,the growth rate of strains and pneumococcus pneumonia mice's mortality were measured.Results 8% dose Sanxiaoyuchuang group had obvious inhibition effects on mice auricle swelling induced by Dimethylbenzene,it had no obvious difference compared with Jingwanhong group.It could also lower the mice earcapillary vessel permeability,inhibit or eradicate the colon bacillus,Staphylococcus aureus,cyanomycosis,Sonne dysetery,typhoid bacillus,and decrease the mortality rate of mice intraperitoneal injected by pneumococcus.Conclusion Sanxiaoyuchuang ointment has good antiinflammatory and bacteriostasis(in vivo and in vitro) pharmacodynamics effects.