1.THE STUDY OF THE SUPERIOR AND INFERIOR ALVEOLAR ARCHES IN CHINESE
Acta Anatomica Sinica 1953;0(01):-
Measurements were made from different landmarks of the superior and inferior alveolar arches in 197 skulls and in 243 mandibles. The data were divided into four groups: 2-5 years old(only deciduous teeth in occlusion); 6-12 years old(first permanent molars in occlusion, but not second molars); 12-17 years old (second permanent molars in occlusion, but not third molars); adults (third molars in occlusion, not including the aged). The results from the observation of the adult specimens are as follows:The indices of alveolar arches are about 118.2 in maxilla; 77.6 in mandible.The average distances from Prosthion (incision in mandible) to Canine point are 19.6mm (left side), 19.8mm (right side) in maxilla; 13.8mm (left side), 14.0mm (right side) in mandible.The distances from canine point to ectomolare (the lateral most margin of the al veolar cavities of second molars in maxilla; of third molars in mandible) are about 33.7mm (left and right sides) in maxilla; 43.7mm (left side), 44.5mm (right side). in mandible.The convergence distance of alveolar arches is 42.9mm in maxilla; 26.4mm in mandible and the convergence angle is 51.5? in mandible; 43.1? in maxilla.It was found that most of the superior and inferior alveolar arches were asymmetrical, the percentages of asymmetry being 82.2 in maxilla and 67.0 in mandible of adults.
2.Analysis of residual stress between core and veneer ceramics by finite element method
Haitao XIN ; Xinyang MA ; Yulu WU
Journal of Practical Stomatology 2010;26(2):173-176
Objective:To study the residual stress distribution through the thickness of bilayered dental ceramic subjected to thermal stress, in order to improve the restoration. Methods: The finite element model of bilayered dental ceramic was set up based on International Organization for Standardization(ISO) 96936:1999. The residual stresses were calculated in viscoelastic and elastic phases during cooling of dental ceramic to analyze the residual stress distribution. Results: The deformation of core was greater than the veneer ceramics during the cooling period of dental ceramic. The residual stress increased with the decreasing of the temperature approaching the interface of core and veneer ceramics. But it decreased with the increasing of the thickness of veneer ceramics.Conclusion: Thermal compatibility of core and veneer ceramics is very important to the residual stress distribution in the bilayered dental ceramic, which may benefit to All-ceramic restorations. The viscoelastic behavior of ceramic should be taken into account in the thermal compatibility.
3.Tissue engineering cbondrogenesis :an experiment study of bone mesenchymal stem cells
Shifeng SONG ; Haitao XIAO ; Wei WU
Orthopedic Journal of China 2006;0(06):-
[Objective]To investigate the chondrogenic ability and feasibility of bone mesenchymal stem cells(BMSCs) in three kinds of cellular carrier.[Method]BMSCs were separated and cultured into the third generation,which were cultivated in three knids of carriers by multi-point injection into and dropping on the surface of carriers(fibrin collagen sponge,group A;fibrin collagen sponge and FGF,group B and gelatin sponge,group C).All samples were analyzed after four weeks.[Result]There were a few chondrogenic cells in group A,much more chondrogenic cells in group B,and no cells in group C.According to analysis with SPSS 10.0 software,There were more cartilage cells(92.75?10.57) in group B than in group A(36.45?8.34).It was statistical different(P
4.Determination of Heavy Metal in Water With Solid Phase Extraction Followed by High Performance Liquid Chromatography
Xianhua WU ; Hong LIN ; Haitao LI
Journal of Environment and Health 1992;0(05):-
Objective To establish a new method for determination of copper, nickel, silver, lead, cadmium and mercury in water with solid phase extraction followed by rapid high performance liquid chromatography. Methods The copper, nickel, silver, lead, cadmium and mercury ions were pre-column derived by tera-(4-dimethylaminophenyl)-porphine (T4-DMAPP). These chelates were enriched by solid phase extraction with C18 cartridge and the 100 times enriched products of were obtained. The chelates were separated on a ZORBAX Stable Bound (4.6 mm?50 mm, 1.8 ?m) by gradient elution with methanol and THF as mobile phase at a flow rate of 2.0 ml/min and detected with photodiode array detector from 350-600 nm. Results The detection limits of nickel, copper, silver, lead, cadmium, and mercury were 3, 2, 4, 3, 1.5 and 3 ng/L respectively, the recovery rates were 92%-104%, relative standard deviations were 2.8%-3.5%. Conclusion This method can be applied to the determination of nickel, copper, silver, lead, cadmium, and mercury in water with good results.
5.Study on changes of bcl-2 expression in apoptosis of PGCl_3 cells induced by DAPK
Jun WU ; Biao LIANG ; Haitao ZHANG
China Oncology 2001;0(05):-
Purpose:To investigate effect on bcl-2 and bax expression in apoptic PGCl_(3) cells and to study the mechanism of apoptosis of PGCl_(3) cells induced by death associated protein kinase(DAPK).Methods:Eukaryotic express vector pcDNA3.1-DAPK was tranfected into High-metastasis Non-small Lung Cancer Cell PGCl_(3).Changes of bcl2 and bax expression were detected with RT-PCR.Phospharation of Bcl-2 was examined with Westernblotting.Results:It was demonstrated that bcl-2 expression was down regulated,but bax expression did not change in apoptosis of PGCl_(3) cells induced by DAPK.Level of Phospharation of Bcl-2 increased.Conclusions:Apoptosis of PGCl_(3) cells induced by over-expression of DAPK may be associated with down regulating of bcl-2 expressio and increase of phospharation of Bcl-2.
6.The gene type of Candida albicans in the oral cavity of Uygur children aged 3 -5 years with high caries
Nan WU ; Haitao DAI ; Jiang XU
Journal of Practical Stomatology 2016;32(2):252-255
Objective:To investigate the oral Candida albicans(CA)distribution in Uyghur children and to explore the gene type of CA in the children with high caries.Methods:The oral CA of 144 Uyghur children aged 3 -5 years was detected and identified by CHROMagar Candida medium culture,biochemical identification and PCR respectively.Gene type in 25 samples of high caries was de-tected by PCR25r-genotyping.Results:CA was found in 35(24.3%)of the children,and 25 of them with high caries.The gene type of CA was divided into type A,B and C,A was the major(72.0%).Conclusion:Oral Candida albicans may be related to childhood caries of Uygur population.Candida albicans with genotype A may be more cariogenic in Uygur children.
7.Determination of Borneol in Aqueous Humor and Perfusate of Rabbit Cornea by Gas Chromatography
Haitao WU ; Youzhi TANG ; Ningsheng WANG
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(06):-
Objective To establish gas chromatography for determining the contents of borneol in aqueous humor and perfusate of rabbit cornea.Methods Naphthalene was used as the internal standard,samples were extracted by ethyl acetate and the chromatographic procedure was carried out in OV-1701 capillary column.The parameters were set as follows:Inject Temp,210 ℃;Oven Temp,30 ℃;FID Temp,250 ℃;Carrier gas:Nitrogen,3.0psi;Hydrogen,45 ml/min;Air,450 ml/min;split ratio,10:1.Results Borneol and isoborneol were well separated from other components.Borneol and isoborneol had an good linearity in the rang of 0.2~20 ?g/ml,R2 was 0.9953 for borneol and 0.9902 for isoborneol;and the intra-day and inter-day RSD were less than 9.68 %and 12.60 %respectively.Conclusion The method is simple and precise,and can be used for determining borneol in ophthalmological preparation.
8.PET Image of Spongiform Leukoencephalopathy From Heroin Vapor Inhalation
Quanshi WANG ; Hubing WU ; Haitao HU
Journal of Practical Radiology 2001;0(05):-
Objective To clarify PET image characteristic of spongiform leukoencephalopathy from heroin vapor inhalation and evaluate PET in the dignosis of the disease. Methods 18 F-FDG PET was performed in six patients with spongiform leukoencephalopathy from heroin vapor inhalation. They developed progressive cerebellar ataxia ,dysarthria and unresponsive in different degree respectively.They were a known drug abuser and had a history of heroin use for 1~3 years.Abstinence of heroin was performed from 1.5 to 3 months in the 2 patients .It was known that they took their heroin not by injection but by inhalation of heated heroin vapours,a method known as "chasing the dragon".Brain CT and MRI revealed some characteristics in all patients. Needle biopsy (n=2) was performed and pathological examination proved spongiform degeneration in the white matter. 13 N-ammonia PET was performed in 1 of 6 patients after 24 hours.Results Brain 18 F-FDG PET revealed bilateral and symmetrical 18 F-FDG accumulation decrease or defect areas invoving the internal capsule behind limb ,cerebral occipital lobes ,and cerebellar hemispheres(cerebellar white matter was severely involved).Brain 13 N-ammonia PET imaging demonstrated abnormal areas of decreased accumulation in the bilateral frontal lobes, bilateral temporal lobes , bilateral occipital lobes, bilateral cerebellar cortex and no 13 N-ammonia accumulation in the bilateral internal capsule behind limb and cerebellar white matter in 1 of 6 patients. The region of the lesion was larger than 18 F-FDG PET imaging in the same patient. Conclusion Spongiform leukoencephalopathy from heroin vapor inhalation has some characteristics on PET ,which are helpful for the diagnosis when combined addictive history.
9.Clinical characters of congenital retinal vascular anomalies
Haitao LI ; Feng WEN ; Dezheng WU
Chinese Journal of Ocular Fundus Diseases 2003;0(05):-
Objective To detect the clinical characters and the classification of the congenital retinal vascular anomalies. Methods Nine cases (12 eyes) of congenital retinal vascular anomalies were exa- mined by ocular examination and fundus fluorescein angiography (FFA), in which 3 cases (4 eyes) were examined by indocyanine green angiography (ICGA) simultaneously. Results The congenital retinal vascular anomalies were located at the posterior pole in 8 cases (10 eyes), and extended to peripheral retina in 1 case (2 eyes). Congenital retinal vascular anomalies were classified as follows: congenital retinal macrovessel (1 case, 1 eye); congenital retinal arteriolar tortuosity (2 cases, 3 eyes); inherited retinal venous beading (1 case, 2 eyes); and congenital prepapillary vascular loops (5 cases, 6 eyes). Four cases (5 eyes) were associated with spontaneous hemorrhage induced by physical exertion (Valsalva maneuver). Conclusion Most of the congenital retinal anomalies are located at the posterior pole, involving arteries and veins, and can be associated with spontaneous hemorrhage induced by Valsalva maneuver.
10.Characteristics of the ocular fundus of Chinese patients with polypoidal choroidal vasculopathy
Feng WEN ; Dezheng WU ; Haitao LI
Chinese Journal of Ocular Fundus Diseases 2003;0(05):-
Objective To investigate the characteristics of the ocular fundus of Chinese patients with polypoidal choroidal vasculopathy (PCV). Methods The photographs of ocular fundus of 42 Chinese patients (48 eyes) with PCV diagnosed by fundus photography, fundus fluorescein angiography (FFA), and indocyanine green angiography (ICGA) were analyzed. Results In 48 eyes with PCV, 35 (72.9%) had subretinal hemorrhage, 13 (27.1%) had yellow-white exudation, 7 (14.6%) had subretinal tuberculum-like focus in tangerine peel at the posterior pole, and 26 (54.2%) had hemorrhagic or serous pigment epithelium detachment (PED). Thirty-six eyes (75.0%) had polypoidal dilations with branching vascular network, and the other 12 (25.0%) had scattered polypoidal dilations without identifiable continuous branching vascular network. The location for these lesions were at the macular region in 30 eyes (62 5%), arcade region in 6 (12.5%), peripapillary region in 3 (6.3%), and combination region in 9 (18.8%). Conclusions Most of the lesions in Chinese patients with PCV locate at the macular region and temporal vascular arcade. Most eyes are characterized by branching choroidal vessels with polyplike terminal aneurysmal dilations in ICGA.