1.Analysis on drug resistance rate and homology of Pseudomonas aeruginosa by ICU nosocomial infection monitoring
International Journal of Laboratory Medicine 2017;38(3):300-301,304
Objective To investigate the drug resistance rate and homology of Pseudomonas aeruginosa isolated from ICU in Mudanjiang Municipal First People′s Hospital from January to June 2015 to understand its prevalence situation in ICU and provide a basis for the rational prevention and control of nosocomial infection.Methods The Vitek-2 Compact fully automatic microbiologi-cal identification instrument was adopted to perform the drug resistance analysis on 126 strains of Pseudomonas aeruginosa from ICU detected by different pathways in the first half of 2015.The homology of bacterial strains was analyzed by pulse-field gel elec-trophoresis(PFGE).Results Two nosocomial infection monitorings were performed in ICU from January to June 2015.Four strains of Pseudomonas aeruginosa isolated from the nurse hand,medicative cart and doorknob of ICU ward all were sensitive strains;122 strains of Pseudomonas aeruginosa were detected in 110 ICU inpatients,in which 38 multi-drug resistant strains were detected from 18 ICU inpatients.The homology analysis was performed in 38 multi-drug resistant strains and 4 strains detected by nosocomial infection monitoring,these strains included 5 groups (A-E),which was dominated by the clone types of A,B and C, while the strains detected by nosocomial infection monitoring all were clone type B.Multiple subtype infection was detected in 4 pa-tients.Conclusion The infection situation of Pseudomonas aeruginosa is serious in ICU,no epidemic outbreak of strains detected by nosocomial infection monitoring exists.The cross prevalence of multiple clone strains exists in inpatients.
2.The value of fibrin monomer combined with Wells′ score in early diagnosis of venous thromboembolic disease
Qi LI ; Hairun YAN ; Hong JIN ; Yan ZHAO ; Zhengliang YANG
Chinese Journal of Laboratory Medicine 2017;40(8):589-592
Objective To investigate the change of the fibrin monomer (FM) level in the venous thromboembolic disease (VTE).To compare the diagnostic value of FM combined Wells score with the other detection methods.Methods In this case control study,121 cases were selected from the patients who were from general and orthopeadic surgery (including thrombosis group in 60 cases and non thrombosis group in 61 cases).The patients were assessed by Wells score.From one day before surgery, Plasma d-dimer (D-D) and fibrin monomer (FM) were periodic measured by CP-2000 d-dimer and fibrin monomer.Evaluation the value of d-dimer,fibrin monomer and fibrin monomer combined with Wells score in diagnosis of venous thromboembolic disease.The receiver operation cure(ROC) was drew to determine the diagnostic performance.Results The plasma FM level of patients with VTE in the thrombus group (26.11±38.34) μg/ml is higher than the non thrombus group (6.56±6.81) μg/ml and the control group (2.37±0.89) μg/ml (t=-3.82, t=-4.78,P<0.01);the sensitivity of FM was lower than the D-D (85% vs 93%);then the positive predictive value was lower than D-D (82% vs 87%) (χ2=27.01,P=0.000)but its specificity and negative predictive value (65%) are both higher than D-D (65% vs 44%)(71% vs 62%)(χ2=11.67,P=0.001);the sensitivity,the specificity,the positive predictive value and the negative predictive value of FM combined Wells score are increased (90%,85%,83%,89%)(χ2=20.95,χ2=16.65,P<0.01).The increased level of FM is earlier than imaging changes, and the elevated of plasma D-D is not obvious in a certain period of time.Conclusions The sensitivity and specificity of FM combined with Wells′ score is higher in the diagnosis of VTE, its prediction value in the diagnosis of VTE is higher.The FM level can be changed in the early stage of VTE, which has a certain value of early diagnosis.
3. Clinical evaluation of thrombus molecular markers in patients with malignant tumor
Yangyang ZHENG ; Hairun YAN ; Qi LI ; Hongliang JI ; Zhengliang YANG ; Qinzheng ZHANG ; Runyao MA
Chinese Journal of Laboratory Medicine 2020;43(1):78-84
Objective:
To explore the early diagnostic value of thrombus molecular markers in thrombosis ofpatients with malignant tumors and to evaluate their risk factors.
Methods:
Diagnostic research.A total of 1366 patients (including lung cancer, breast cancer and colorectal cancer,) were randomly selected in the Red Flag Hospital of Mudanjiang Medical College and Mudanjiang Cancer Hospitalfrom September 2009 to February 1919. Among them, 562 were males and 804 were females with average age (59.45±15.10) years old. The control group consisted of 70healthy donors (35 males and 35 females, with an average age of (49.60±19.12) years old), including 69 cases of venous thrombosis (thrombotic group, 32 males and37 females, with an average age of (61.20±15.71) years old).Chemoluminescent enzyme immunoassay was used to detect thromboregulatory proteins(TM), thrombin-antithrombin complexes(TAT), tissue plasminogen activators/inhibitors -1 complexes(t-PAIC), plasminase-anti-fibrinolysis complexes(PIC) in venous plasma. According to the sensitivity and specificity of each marker, the receiver′s work characteristic curve was drawn to evaluate its diagnostic performance. Cox regression analysis was used for single-factor and multi-factor risk analysis.
Results:
The incidence of venous thromboembolism(VTE) in patients with different types of malignant tumors was statistically significant, with lung cancer being the highest, followed by colorectal cancer and breast cancer(
4.Study on correlation between plasma homocysteine level and prognosis in patients with acute myocardial infarction
Yan XUE ; Hairun LIU ; Zicong YANG ; Tao ZENG ; Ling LIU ; Nengwen XU ; Ying SHI
Chongqing Medicine 2017;46(31):4342-4344,4347
Objective To investigate the correlation between plasma homocysteine(Hcy) and long term occurrence of main adverse cardiac events(MACE) in the patients with acute myocardial infarction(AMI).Methods A total of 326 inpatients with AMI in the cardiology department of this hospital from Mar.2012 to Dec.2014 were continuously included.The related laboratory biochemical indicators such as Hcy were detected according to the standard flow process by the hospital clinical laboratory department.The Hcy level and MACE occurrence rate were performed the receiver operating characteristic(ROC) curve analysis.The research subjects were divided into the Hcy(L) group and Hcy(H) group according to the cut-off value 11.69μmol/L.The base line data of all subjects were collected and the follow up was conducted for recording MACE.Results The plasma N-terminal pro-brain natriuretic peptide (NT-ProBNP) level in the Hcy(L) group was significantly lower than that in the Hcy(H) group[-(501.46-±-118.35)pg/mL vs.(1 324.11 ± 523.13) pg/mL,P =0.02],while the left ventricular ejection fraction(LVEF) was significantly higher than that in the Hcy(H) group[(55.23 ± 9.48) % vs.(50.79 ± 10.68) %,P =0.03].After 1-year follow up,LVEF in the Hcy(H) group was significantly decreased compared with the base line [(45.32±10.18)% vs.(50.79±10.68)%,P<0.05],moreover the MACE occurrence risk in the Hcy(H) group was significantly higher than that in the Hcy(L) group,the difference after correcting the related confounding factors had statistical significance (P=0.048),but LVEF and minor axis shortening rate of left ventricle(FS) in the Hcy(L) group had no obvious difference compared with the base line data[(54.43 ± 10.68)% vs.(55.23± 9.48) %,(28.56±6.21) % vs.(29.22±5.30) %,P>0.05].Conclusion The risk of long term MACE occurrence in the patients with AMI complicating plasma high Hcy level is obviously increased compared with that in the patients with plasma low Hcy level and the prognosis is poor,prompting that plasma high Hcy level is one of possible independent predicting factors for poor prognosis in the patients with AML But its action mechanism and whether the Hcy interventional therapy improving prognosis of AMI patients still need to further study.
5.Correlation between interleukin-27 polymorphisms and coronary heart disease in the Guangxi Zhuang Population
Yan XUE ; Hairun LIU ; Zicong YANG ; Jianyong LU ; Xinshun HUANG ; Ying HUANG ; Yu LIU ; Ling LIU ; Ying SHI
Chinese Journal of Geriatrics 2020;39(6):632-635
Objective:To investigate the correlation between interleukin-27(IL-27)polymorphisms and coronary heart disease(CHD)in the Guangxi Zhuang population.Methods:A total of 1 218 subjects were enrolled in this case-control study, and 571 cases were in the CHD group and 647 were in the control group.The Sequenom MassArray system was used to genotype IL-27 single nucleotide polymorphisms(SNPs), and other biochemical parameters were tested by using the standard protocol in the laboratory of our hospital.The correlation between IL-27 SNPs and CHD was evaluated by Logistic regression analysis, and risk factors including age, smoking, drinking, history of hypertension, history of diabetes mellitus and family history of CHD were adjusted in this model.Methods:Logistic regression analysis results indicated that the risk of CHD was elevated in subjects with the CC genotype of IL-27 rs428253, compared with those with the GG genotype( OR=2.18, 95% CI: 1.09-4.37, P<0.05). After adjustment for the related risk factors, the risk of CHD also increased in subjects with the CC genotype of IL-27 rs428253, compared with those with the GG genotype( R=2.82, 95% CI: 1.31-6.07, P<0.05). IL-27 rs4740, rs17855750, rs181206 and rs40837(all P>0.05)polymorphisms had no correlation with CHD in the Zhuang population, and there were no significant differences in C-reactive protein, total cholesterol, triglyceride or low-density lipoprotein cholesterol levels between CHD patients and controls with different genotypes of IL-27 rs428253(all P>0.05). Conclusions:IL-27 rs428253 polymorphism is correlated with the risk of CHD in the Guangxi Zhuang population.