[Objective]To review the treating effect of cases of the developmental dysplasia of the hip in old age children by a one-stage combined Pemberton operation and femoral shortening and investgate if the hip joint stiffness rate and ischemic necrosis rate of femoral head after operation are reduced. [Method]From June 2002 to Decempber 2006, 136 hip congenital dislocation of 101 old age children (7 years to 14 years) were treated by Pemberton operation and femoral shortening,and were followed-up from 1.5 years to 4 years(average, 2 years and 7 months).[Result]Thirty-one cases can only bend their hip joint below 90 degrees, accounting for 22.79% of all. Thirty-eight ischemic necrosis of femoral head occurred which accounted for 27.94%.The rate of ischemic necrosis of the femoral head and hip joint stiffness of old age children after operation were reduced obviously according to the standard by Salter.[Conclusion]Pemberton operation and femoral shortening are preferred in treating the developmental dysplasia of the hip of old age children.It can reduce the rate of ischemic necrosis of femoral head and hip joint stiffness.

Objective To study the physiology of sleeping in a swine model with obstructive sleep apnea syndrome (OSAS) reproduced by high altitude and hypoxia. Methods Twelve male minipigs were randomly assigned to 2 groups, named A and B. The pigs of Group A were placed in Room 1, which was a double-room altitude chamber, and the pressure in it was adjusted to 53.9 kPa with an oxygen concentration of 10.0%~11.2%. The pigs of Group B were kept in Room 2 with altitude of sea level and oxygen concentration of 21%. All the animals were kept in their respective rooms for 6 hours per day and for 22 consecutive days. On the 23rd day sleeping was monitored, at the same time the movements of chest and abdomen, respiratory airflow, heart rate, and pulse oxygen saturation (SpO_2) were recorded. Apnea/Hypopnea Index (AHI), Hypopnea Index (HI), and average SpO_2 were derived from analysis of the data of sleeping monitoring. Pigs were sacrificed after the experiment, and pharyngeal tissue was harvested for pathological examination. Results The AHI (16.1?8.3 vs 2.3?1.6), HI (4.3?3.1 vs 1.3?1.0), SpO_2 (86.7?3.6 vs 94.5?1.8) of pigs of group A showed were have significant differences compared with that of the control (P

Objective To summarize the clinical and imaging characteristics of primary Rosai-Dorfman disease (RDD)in central nervous system in order to understand well its features and improve the diagnosis.Methods The clinical and imaging features in 3 patients with RDD in CNS proved by pathology were retrospectively analyzed in combination with the related literatures.Results The lesions in 2 patients occurred in brain and other lesion in 1 occurred outside the brain.Iso-or slight hyperintensity on T1 WI and marked enhancement of the lesion were found.Single lesion was found in 2 patients near the dura mater and located near the superior sagittal sinus or cervical spinal cord.Multiple lesions in 1 patient were located in the lateral ventricle.Conclusion It’s suggested that single or multiple lesions in dural or spinal meninges located in the midline or near the intracephalic venous system would be considered as RDD in CNS.

Objective To evaluate the value of fat-saturated three-dimensional fast imaging with steady state procession(FS-3D-FISP)sequence in detecting hyaline cartilage defects in osteoarthritis in rabbits.Methods Osteoarthritis was induced in eighteen male rabbits by injecting papain into the left knee joints.The same volume of sterile saline solution was injected into right knee joints as controls.MRI of knee joints was done at 1,2 and 4 weeks after injection using FS-3D-FISP sequence.The the rabbits were killed and pathohistological examinations of the femoral condylars and tibial plateaus were performed.The cartilage changes were observed by MR image according to the Outerbridge method and by pathology using Collins staging.Results There was closely correlation between MR imaging and pathology in grading of cartilage injury in grade I～II and grade III～IV(P＜0.001).Conclusion FS-3D-FISP can evaluate accurately the degree of articular injury.

Objective To research the clinical therapeutic effect and side effect of gesterol- pingyangmycin cream in interventional therapy of hysteromyoma. Methods 110 cases of hysteromyoma underwent interventional therapy who were divided randomly into two groups:group A with polyvinyl alcohol particle and group B with gesterol-pingyangmycin cream .By percutaneous catheterization in bilateral uterine arteriae using the technique of Seldinger,polyvinyl alcohol particle or gesterol-pingyangmyci cream were injected to blodk up the blood supply of hysteromyoma.The volume of hysteromyomas was measured with B-mode ultrasonography prior and post-treatment.The reaction of post-treatment and the changes of menstrual blood volume were observed . Results The menstruation turned to normal and or menstrual blood volume reduced obviously in two groups , the total effective rate was 95.49 . The volume of hysteromyomas diminished to 45.81 and 51.80 in 3 and 6 months,respectively after treatment.There was significant difference between prior and post-treatment(P

Staphylococcus epidermidis is the most important member of the coagulase-negative staphylococci and one of the most abundant colonizers of human skin.S.epidermidis is a bacterial species that is commonly described as relatively innoc-uous,which is in part due to the notion that it lacks secreted toxins with limited capability of infection.S.epidermidis pro-duces a series of molecules that provide protection from host defenses,including many proteins and exopolymers.Further-more,recent research has identified a family of cytolytic peptidesin S.epidermidis,the phenolsoluble modulins (PSMs),and the notion that S.epidermidis is virtually toxin-free had to be somewhat revised.

Objective To investigate the relationship of the killer cell immunoglobulin-like receptor (KIR) gene polymorphism with Hashimoto′s thyroiditis(HT). Methods One hundred HT patients and 260 randomly matched healthy controls were enrolled to detect the KIR genotype. The genomic DNA were extracted, and 15 selected KIR genes, KIR2DL1-5, KIR3DL1-3, KIR2DS1-5, KIR3DS1 and pseudogene KIR2DP1, were determined by a polymerase chain reaction using sequence-specific primers (PCR-SSP). Results The frequency of KIR2DL5 gene was significantly lower of the patient group than that of the control group (0.200 vs 0.312, RR=0.64, P<0.01). Conclusion There may be an association between pathogenesis of HT and KIR2DL5 gene.

A survey was conducted on a sample of 67 general practitioners ( GPs ) receiving in-service training in Beijing in 2012.Hypertension knowledge mastering and training status were investigated through a self-administered questionnaire .Results showed that the accuracy rates of the 4 domainitems (basic knowledge and skill, lifestyle modification, drug therapy, health management ) were 42.3%(340/804), 48.0%(225/469), 27.8%(96/335) and 70.6%(473/670), respectively with an overall accuracy rate of 49.8%(1 134/2 278).Trainees from urban scored higher than those from rural areas (P<0.01).About one third of trainees indicated that the 3 main teaching sessions they took part in did not cover the content of hypertension management .The hypertension guideline ( primary care version 2009) was most frequently used for training GPs , but only 33.9%(21/62) accessed such training .The results indicate that hypertension knowledge level seems to be generally low among GPs on in-service training in Beijing and the current training situation need to be further improved , particularly for those from rural areas .

Objective To study the proteins related to aging in aortic of old rats for laying the foundation of further study of aging mechanism.Methods The rat model of aging was built,and all model rats were divided into 4 groups:the adult group(9 weeks),the old group(12 months)of WistarKyoto (WKY) rats,and the age-matched spontaneously hypertensive rats (SHR).Blood pressure of 4 groups was observed.Morphological change of aorta was observed by HE staining.Differential proteins were identified by isobaric tags for relative and absolute quantitation,(iTRAQ)-coupled liquid chromatography and tandem mass spectrometry technology.Part of differential proteins was subsequently detected by real time PCR and Western blot.Results The mean SBP of the old group SHR was higher than WKY of 97.1％ (t=39.00,P＜0.05),and the adult group of SHR was also higher than WKY of 5.4％(t=3.64,P＜0.05).Compared with the adult group,aging change in the aortic morphology of old SHR and WKY were shown in HE staining,and the change in SHR rats was more marked.7 proteins related to aging were identified by Mass spectrum analysis,and they were Profilin-1,Prelamin A,HSP70,creatine kinase-M,Fibulin-5,eIF5A and Prohibitin.Part of differential proteins was subsequently confirmed by real time PCR and Western blot.Prelamin-A was up-regulated in the old group of WKY and SHR (0.15±0.01 vs.0.45±0.04,0.34±0.02 vs.0.78±0.06) (t=12.67,12.06,all P＜0.01),Prohibitin was down-regulated in the old group of WKY and SHR(1.34±0.05 vs.1.01± 0.06,1.24±0.05 vs.0.88±0.08) (t=7.41,7.09,all P＜0.01).Profilin-1 was up-regulated in the old group of WKY and SHR (9.12±0.4 vs.20.76±0.8,16.84±0.5 vs.55.16±0.9) (t=22.55,64.46,both P＜0.01),and Profilin-1 expression in the old group of SHR was higher thanWKY (55.16±0.90 vs.20.76±0.8,t=49.49,P＜0.01).Conclusions Differential proteins of the old rat aorta are identified through the comparative proteomics method.These differential proteins will provide new targets for the prevention and control of vascular aging.

Objective To explore the changes on the value of CVP with different body postures after upper abdominal surgery in order to provide accurate basis for better monitoring of central venous pressure(CVP). Methods The CVP, blood pressure,pulse,respiration of 43 patients after upper abdom-inal surgery with indwelling central venous catheter were measured under supine position, 15°dorsal ele-vated position and 30°dorsal elevated position.The data were collected for correlation and regression analysis. Results The CVP value increased with the elevation of body postures,the CVP value under supine position was linearly positively correlated with the other two positions(P<0.01).The regression e-quation of supine position and 15°dorsal elevated position was:Y=1.009X1+0.811; the regression equation of supine position and 30°dorsal elevated position was:Y=1.005X2+1.630. Conclusions Through moni-toring the CVP value under 15°dorsal elevated position and 30°dorsal elevated position,and substitute it in equation,we can evaluate the CVP value under supine position.