Background and purpose:Nausea and vomiting were the most common side effects of chemotherapy and they are difficult to control especially in terms of delayed nausea and vomiting induced by CDDP. Acupuncture treatment is popularly the traditional medical method used in China,being economic and easy to carry out.This research is to study the role of acupuncture treatment in the prevention of NP regimen induced delayed nausea and vomiting. Methods:26 patients with NSCLC were enrolled into a randomized double blind crossover trial. The patients received either hydrochloride ondostrone+Dexm+acupuncture treatment(the first cycle of chemotherapy) →hydrochloride ondostrone+Dexm (the second cycle of chemotherapy)(defined as AB group) or hydrochloride ondostrone+Dexm (the first cycle of chemotherapy)→hydrochloride ondostrone+Dexm+acupuncture treatment (the second cycle of chemotherapy)( defined as BA group).Results:Twenty six patients were evaluated. The complete controls of nausea for the AB group with acupuncture treatment were 96.15%, 96.15%, 76.92%, 84.62%, 92.31% and 100.00% for day 1-6, respectively, for the BA group without acupuncture treatment were 100.00%, 76.92%, 57.69%, 34.62%, 65.38% and 76.92%, respectively. There were statistically difference in terms of the control of nausea for day 3-5(P

Objective To study the radiation protection and its molecular mechanism of the polysaccharide from Monostroma nitidum. Methods The mice were randomly divided into the normal group, the model group and 3 treatment groups. Polysaccharide from Monostroma nitidum was applied to the treatment group for 15 d before irradiation with 6.0 Gy rays. 30-day survival rate was observed. The counts of peripheral white blood cell, thrombocytes and erythrocytes were measured. The activities of the antioxidant enzymes SOD and GSH-Px were assayed , and the damage to the cell membrane was studied by measuring the lipid peroxides MDA levels. The spleen index and transformation response of splenic lymphocyte were determined. Results 30 day survival rate significantly increased in the polysaccharide groups. The animal experiment showed the counts of peripheral white blood cell, thrombocytes and erythrocytns were significantly and more rapidly recovered in the polysaccharide group after irradiation. The polysaccharides markedly increased the activities of GSH-Px and SOD and decreased the levels of MDA in the blood. In the irradiated mice, the polysacchaxide significantly increased the spleen index and transformation response of splenic lymphocyte when compared with irradiation mice.Conclusion The present observations suggest that the polysaccharide from Monostroma nitidum had a marked redioprotective effect on irradiated mice and that the biological response might be caused by the hematopoiesis recovery, antioxidant effect and immune activation.

Objective To evaluate the therapeutic results of expandable stent for treatment of atherosclerotic renovascular obstructive disease. Methods 15 patients (10 men and 5 women, 41～75 years old; mean age, 52 years) with renal arterial hypertension underwent renal stent agioplasty including renal arterial stenosis 89% (n=13) and fully obstruction without function in 2, of which 2 patients had bilateral involvement. The stenotic range of all arterial segments showed 60% to 90% width of the normal arterial diameter. 16 stents were implanted under the guidance of flouroscopy. The most of stents implanted were Palmaz (n=12, 75%) with regular clinial and angiographic follow up. Results Technical success (residual stenosis

Objective To study the physiology of sleeping in a swine model with obstructive sleep apnea syndrome (OSAS) reproduced by high altitude and hypoxia. Methods Twelve male minipigs were randomly assigned to 2 groups, named A and B. The pigs of Group A were placed in Room 1, which was a double-room altitude chamber, and the pressure in it was adjusted to 53.9 kPa with an oxygen concentration of 10.0%~11.2%. The pigs of Group B were kept in Room 2 with altitude of sea level and oxygen concentration of 21%. All the animals were kept in their respective rooms for 6 hours per day and for 22 consecutive days. On the 23rd day sleeping was monitored, at the same time the movements of chest and abdomen, respiratory airflow, heart rate, and pulse oxygen saturation (SpO_2) were recorded. Apnea/Hypopnea Index (AHI), Hypopnea Index (HI), and average SpO_2 were derived from analysis of the data of sleeping monitoring. Pigs were sacrificed after the experiment, and pharyngeal tissue was harvested for pathological examination. Results The AHI (16.1?8.3 vs 2.3?1.6), HI (4.3?3.1 vs 1.3?1.0), SpO_2 (86.7?3.6 vs 94.5?1.8) of pigs of group A showed were have significant differences compared with that of the control (P

Objective To investigate the pathologic features of ganglioglioma/gangliocytoma.Methods 19 cases of gangliogliomas and gangliocytomas were studied by HE stain, immunohistochemistry and ultrastructural observation.Results (1) The tumor cells presented widespread, derangement distribution, and conglobation in some regions. (2) The tumor cells were similar with ganglion cell, showing multipolar in feature, and had large, irregular nucleoli. The nucleoli were clear. Some tumor cells were bi-nucleated. (3) Neurogliocytomas were Ⅱ～Ⅲ grades according to the standard published by WHO. (4) Immunoreactions showed GFAP (-), S-100 (+) and Synaptophyein (+) in neoplastic cells. (5) Ultrastructure observation showed cytolysosome, ribosome and rough endoplasmic reticulum in the cytoplasm of the neoplastic cells. Abundant mitochondria and dense neuroendocrine granules were also found in these cells.Conclusions Ganglioglioma/gangliocytoma is a rare tumor of central nervous system. Its diagnosis mainly depends on histopathology, special markers of immunohistochemistry and ultrastructural features. The property of neoplasma is related to the component of neurogliocytoma.

BACKGROUND: Nerve root could be directly or indirectly entrapped due to lateral backward prolapse of nucleus pulposus of intervertebral disc, as well as parenchymal inflammatory tumefaction of the soft tissues in nerve root tube. But the factors and their severity related to cervical nerve entrapment at cervical intervertebral foramen and spinal nervous sulcus still need further studies.OBJECTIVE: To investigate the relationship between the occurrence rate of cervical nerve entrapment at cervical intervertebral foramen and spinal nervous sulcus and the severity of cervical syndrome.DESIGN: A single sample study.SETTING: Department of Anatomy, Department of Geriatrics of the Affiliated Hospital, and Department of Teaching Affairs, Chengde Medical College.PARTICIPANTS: The research was conducted in the Department of Anatomy, Chengde Medical College. Totally 120 sides of 60 cadavers(28 males and 32 females) were provided by the Department of Anatomy.METHODS: The outside diameters of cervical intervertebral foramen and spinal nerve root, as well as the width of external opening of spinal nerve sulcus and transverse diameter width of the anterior branch of intra-spinal nerve were measured, and the data were dealt with statistically.MAIN OUTCOME MEASURES: The ratio of transversal diameter of the forepart of cervical nerves to the width of spinal nerve sulcus; the ratio of the peripheral diameter of cervical nerve roots to the size of intervertebral foramen; the occurrence rate where the ratio was ≥ 1.RESULTS: The ratio between cervical nerve root and intervertebral foramen was significantly larger than that between spinal nerve root and spinal nerve sulcus( t = 2.66, P ＜ 0.01 ). The occurrence rate of cervical nerve entrapment at the intervertebral foramen(24.6% ) was higher than that in spinal nerve sulcus(6.3% ), and the difference had a considerable statistical significance(x2 =6.95, P ＜ 0.01).CONCLUSION: It is suggested that the spinal nerve may be entrapped in the cervical intervertebral foramen and the spinal nervous sulcus, and the spinal nerve lesions in the cervical intervertebral foramen was more serious than in the spinal nervous sulcus. Therefore, in clinic, when diagnosing and treating cervical spondylosis, while nerve entrapment at the intervertebral foramen is fully considered, the possibility of nerve entrapment at the spinal nervous sulcus should not be neglected.

[Objective] To study the analgesic, anti-diarrhea and anti-inflammatory effects of Wulian compound extract. [Method] To study the analgesic effect of Wulian compound extract on mice with hot-plate test, writhing test, and formaldehyde stimulation. To study the anti-inflammatory effect of Wulian compound extract by mice ear-swol en induced by xylene and abdominal cavity capil ary Permeability text .To study the anti-diarrhea effect of the Wulian compound extract with the method of diarrhea induced by castor oil and senna. [Results] Wulian compound extract can inhibit the pain caused by high fever, acetic acid and formaldehyde. Inhibit the xylene induced ear swel ing of mice and increase of celiac capil ary permeability induced by acetic acid. At the same time Wulian compound extract can prolong diarrhea latency induced by the senna and castor oil, reduce the frequency of diarrhea. [Conclusion]Compound Wulian extracts have significant antidiarrheal effect, anti-inflammatory, analgesic.

Objective To determine the difference of global indices of retinal thickness at posterior pole in primary and suspected glaucoma. Methods Forty five global indices of analysis on retinal thickness at posterior pole in every case, including 12 cases of primary open angle glaucoma and 11 cases of suspected glaucoma were obtained by advanced retinal thickness analyzer. Every index was also compared. Results There were significant differences between primary and suspected glaucoma in foveal shape deviation (FSD), foveal corrected thickness deviation (FCTD), foveal fixation corrected thickness deviation (FFD), foveola thickness deviation (VTD), corrected foveola thickness deviation (CVTD), peri foveal abnormally thin area (PFATN), posterior pole pattern deviation (PPPD), and posterior pole abnormally thin area (PPATN). Conclusion There are significant difference of morphologic indices of retinal thickness at posterior pole between primary and suspected glaucoma.

Objective To evaluate the performances of high-sensitivity cardiac troponin I ( cTnI ) on VITRO ECIQ with enhanced chemiluminescence method .Methods This verification study validated the limited of detection,function sensitivity,imprecision,linearity of the high-sensitivity cardic troponin used VITROS ECIQ according to the document EP-17A, EP-6A,EP-15A published by Clinical and Laboratory Standards Institute (CLSI) and determined 99th percentiles.All 652 patients with chest pain on immediate admission in TEDA International Cardiovascular Hospital during January to November 2013 were enrolled in this study.Including 323 cases of acute ST segment elevation myocardial infarction and non ST segment elevation myocardial infarction patients as the case group , exclude 329 cases of other diagnosis ,303 cases of apparent normal people as control group .The receiver operating characteristic curve was used to evaluated the sensitivity and the specificity of cTnI . Non-normal distribution of quantitative data were used nonparametric test Mann-Whitney U, With P<0.05 for the difference was statistically significant .Results The LoB was 0.006 5 ng/ml and the LoD was 0.015 5 ng/ml;the FS was 0.016 76 ng/ml;repeatability CV
was 1.73 % -2.33 %, reproducibility CV was 4.93% -9.96%.The imprecision were lower than that declared by assay producer.The linearity was 0.015 5-78.4 ng/ml(R2 =0.999 9); the 99th percentile reference value was 0.017 ng/ml.The area under the curve ( AUC) of cTnI was 0.986,95%CI 0.973 -0.994,with the cut-off value as 0.017 ng/ml, the diagnostic sensitivity and specificity in CIN were 90.09%and 99.34%.Compared between STEMI and NSTEMI groups after admission , the levels of cTnI had no significantly difference , Z were -0.485, P >0.05;compared between STEMI and control groups after admission, the levels of cTnI had significantly difference , Z were -19.567,P<0.001;compared between NSTEMI and control groups after admission , the levels of cTnI had significantly difference , Z were-14.598,P<0.001.Conclusions High-sensitivity cardiac troponin I detection by VITROS ECIQ with enhanced chemiluminescence method has good performances of sensitivity , linearity, specificity, which meet the clinical needs.

Objective To analyze the protein expression changes of retina in diabetic mice using isobaric tags for relative and absolute quantitation (iTRAQ) approach and to study proteins of apoptosis.Methods 8 diabetic mice were chosen as the diabetic model group (DM group),8diabetic mice as the normal control group.The animals were housed in wire-bottomed cages and received normal pellet chow and tap water in a constant environment.After 10 weeks,all mice were killed,and their retina were dissected.After hematoxylin and eosin(H&E) staining,the sections of retina were examined using light microscopy.The changes of protein expression in retina were studied using iTRAQ approach.Expression of apoptosis associated proteins was analyzed using ingenuity pathway analysis (IPA).Results Compared with control group,mice retina in DM group developed looser structures,tissue edema and obvious telangiectasia under light microscopy.Using iTRAQ approach,a total of 348 differential proteins were identified.Among those proteins,16 proteins were related with apoptosis,including Ataxin-10,Protein NDRG1,mucin-4,Aquaporin-1 and annexin A4,etc.There were 8 apoptosis-related proteins in retina with up-regulation,and the other 8 proteins with down-regulation in the DM group.The relationship between these proteins were analyzed and charted by IPA.Conclusions Apoptosis may be involved in the development of diabetic retinopathy.The identification of the apoptosis-related proteins will be helpful for the further study.