Cancer stem cell (CSC) is the source of tumor development and metastasis, and may become the target of cancer therapy in the future. Niche refers to a micro-environment that makes up the basis of stem cell existence. Niche is involved in tumor development by regulating the growth and changes of CSC. It is also closely related to tumor migration and metastasis. Eradicating CSC through modifying niche may be a new therapeutic strategy for curing cancer.

OBJECTIVE To screen for a simple,accurate and no-traumatic detecting method of Helicobacter pylori(Hp). METHODS Hp in gastric fluid and dental plaque was detected with fluorescent antibody method,bacterial culture,urease test and Hp diagnosis card at the same time in 62 cases with gastric and duodenal disease.The gather of gastric fluid applied the capsule method. RESULTS The detective rates of Hp in gastric fluid by four methods were 85.5%,9.7%,61.3%,and 56.5%;the detective rates of Hp in dental plaque by four methods were 88.7%,25.8%,69.4%,and 90.3%,respectively. CONCLUSIONS Fluorescent antibody method combined with capsule method detecting Hp in gastric fluid is specific,sensitive and without trauma.Thus,it is suggested to be used clinically.

Objective To understand the empiric antimicrobial use in patients with pyelonephritis in a hospital,and pro-vide reference for clinical rational antimicrobial use.Methods Data of 620 patients with pyelonephritis admitted to the nephrology department of a hospital between January 2011 and September 2014 were collected,application of antimicrobial agents,coincidence between empiric antimicrobial use and antimicrobial susceptibility testing results in patients with different diseases and different ages were analyzed.Results Before antimicrobial susceptibility testing results were reported,620 pa-tients use 625 times of antimicrobial agents,5 of whom used two kinds of antimicrobial agents at the same time,8 varieties in 15 types of antimicrobial agents were involved,the most frequently used antimicrobial agents were third generation ceph-alosporins,cephamycins,and fluoroquinolones.The overall,partial,and non-coincidence rate between antimicrobial use and antimicrobial susceptibility testing results were 64.32%(n=402),8.32%(n=52),and 27.36%(n=171)respective-ly.The overall coincidence rate in patients with acute pyelonephritis was higher than those with chronic pyelonephritis (77.61% [n=357]vs 58.79%[n=97],P <0.05).The overall coincidence rate in patients <50 years old and ≥50 years old were 68.12%(156/229)and 75.25%(298/396)respectively,there was no significant different between two groups (χ2 =2.93,P =0.09).Conclusion The non-coincidence rate between empiric antimicrobial use and antimicrobial suscepti-bility testing results is high,measures needs to be taken to improve the empiric antimicrobials use.