It expounds the knowledge of causa morbi and mechanism of insomnia, viewing that it is the result of complex reasons functioning to the body, its attack is to some extent related with individual constitution and characteristics, its mechanism is multiple too, the pathology shows deficiency, heat, sputum and stasis.

BACKGROUND: Being a kind of anti-oxidizing agent, vitamin E has the function of eliminating free radical and reducing the free radical injury of antioxidant enzyme in sports, which can alleviate appearance of fatigue and improve sports ability. OBJECTIVE: Though the intervention of vitamin E upon exercise-induced muscle damage (EIMD), the study was designed to investigate the effect of vitamin E on the morphology of EIMD and to provide the experimental basis of research on morphology and mechanism of EIMD. DESIGN, TIME AND SETTING: A randomized block animal experiment was performed in the Key Laboratory of General Administration of Sport of China in Shenyang Sport University and the Microelectronic Laboratory of Chinese Medical University from May to October 2007. MATERIALS: A total of sixteen male Sprague Dawley rats were randomly divided into four groups: control, exercise, normal saline, and vitamin E groups. METHODS: The rats in the vitamin E group was administered (i.p.) 1-1.2 mg/kg vitamin E capsules once every eight hours four times one day before experiment, and the amount of injecting volume was 4 mL/kg. The rats in the normal saline group were administered (i.p.) normal saline with same volume and time. The rats in the exercise group only took some exercises without administering vitamin E or normal saline. The rats in the control group received not any intervention and treatment. The injury model was established by taking a bout of exhaustive downhill running. The triceps brachii muscles extracted from the right side of rats after exercise were prepared for electron microscope specimens. MAIN OUTCOME MEASURES: The arrangement of myofibrils and sarcomeres, the changes of Z-line, and the morphology changes of cell membranes, nucleus, mitochondria, sarcoplasmic reticulum, transverse tubule, and satellite cells were observed. RESULTS: In the vitamin E group, the muscular ultrastructures of EIMD were improved obviously. Though the sarcoplasmic reticulum showed edema, the regular arrangement of myofibrils was observed. The light and dark zone of Z-line and nucleus appeared clearly. CONCLUSION: Supplement of vitamin E could decrease the muscular morphological changes of EIMD after eccentric exercise. The arrangement of myofibrils was more regular and sarcomeres appeared more clearly. The function might relate to the antioxidation and promoting synthesis of proteins of vitamin E.

BACKGROUND:It has been shown to exhibit the increase of free radical has relationship with exercise-induced skeletal muscle injury.As a kind of antioxidant,vitamin E has the ability of removing radicals,which can relieve antioxidase damage,and delay fatigue,eventually,improve the motor ability.OBJECTIVE:From the angle of free radical metabolism,to explore the effect of vitamin E on malondialdehyde(MDA)and superoxide dismutase(SOD)of mitochondria in skeletal muscular cell after eccentric exercise,additionally,to clarify the mechanism of vitamin E on reducing the skeletal muscle damage.DESIGN,TIME AND SETTING:The randomized grouping experiment of animal was carried out in the Key Laboratory of General Administration of Sport of China in Shenyang Sport University and the Key Laboratory of China Medical University from May to October in 2007.MATERIALS:A total of 48 male,Sprague Dawley rats,weighing(304?12)g were randomly divided into the of control,sport,physiological saline,and vitamin E groups,with 12 animals in each group.METHODS:Rats in the vitamin E group was administered(i.p.)1-1.2 mg/kg vitamin E capsules once per eight hours,four times one day before experiment,and the amount of injecting volume was 4 mL/kg.The rats in physiological saline were injected by physiological saline,with the same injection methods of the vitamin E group.The models of injury were established by a bout of exhaustive eccentric exercise on a treadmill.After exercise,triceps brachiies were moved from the right side of rats.MAIN OUTCOME MEASURES:The content of MDA and activity of SOD were measured with the reagent-boxes and 6010 UV-seen spectrophotometer.RESULTS:The values of MDA,SOD of mitochondria in triceps brachiies cells in each group were greatly increased after 24 hour eccentric exercise(P 0.05),the value of MDA in the vitamin E group was greatly decreased(P

Objective To establish a new cell resource of stem cell transplantation for heart failure therapy,by using cardiomyocytes from rat marrow mesenchymal stem cells(MSCs). Methods MSCs were isolated and cultured from rat bone marrow,then induced by 5aza(5,10,15,20 ?mol/L) for 24 hours.The cultured cells were observed with phase-contrast microscopy.The immunohistochemical technique and laser scanning confocal microscopy(LSCM) were applied for revealing the expressions of desmin,?-sarcomeric actin and C-TnT.The induced cells were evaluated by a transmission electron microscope.GATA4 and(?-MHC) expressions were detected by relative quantitative RT-PCR after 7,21,28 days of inducement respectively. Results MSCs induced in vitro by 5-aza could be identified by the positive staining for desmin,?-sarcomeric actin and C-TnT.The number of cells stained positively in the 5 ?mol/L was greater than that of other groups.Transmission electron microscope showed that paralleled myofilaments were formed.RT-PCR assessment showed that the differentiated cells began to express GATA4 from day 7 to day 28 of differentiation and began to express ?MHC from day 21 to day 28 of differentiation.Conclusion Cardiomyocyte-like cells can be generated from MSCs in vitro and the marrow mesenchymal stem cells are one of the best resources of donor cardiomyocytes.

Objective:To analyze routine blood test results of pregnant women at different periods of pregnancy and evaluate the health status of pregnant women.Methods:A total of 240 pregnant women with anemia who underwent antenatal care or delivery in Department of Obstetrics and Gynecology, The People's Hospital of Yuhuan from January 2018 to June 2019 were included in this study. They were divided into three groups according to different periods of pregnancy with 80 women in each group: first trimester, second trimester, and third trimester. Additional 80 non-pregnant women who concurrently received health examination were included as controls. Routine blood test results were compared among groups. The incidence of microcytic anemia and macrocytic anemia at different periods of pregnancy was determined.Results:The incidence of microcytic anemia in the third trimester group [3.75% (3/80)] was significantly lower than that in the first trimester group [26.25% (21/80)] and the second trimester group [25.00% (20/80), χ2 = 15.882, 14.675, both P < 0.05]. The incidence of macrocytic anemia in the third trimester group [22.50% (18/80)] was significantly higher than that in the first trimester group [1.25% (1/80)] and the second trimester group [3.75% (3/80), χ2 = 17.260, 12.333, both P < 0.05]. Red blood cell count and hemoglobin and hematocrit levels in the peripheral blood of pregnant women in the first, second and third trimester groups were significantly lower compared with the control group ( t = 8.579, 16.781, 13.964, 10.154, 15.891 and 15.512, all P < 0.05). Red blood cell count, hemoglobin and hematocrit levels in the second trimester group were significantly lower than those in the other three groups (all P < 0.05). In the first, second and third trimester groups, white blood cell count and percentage of neutrophils were increased, and platelet count was decreased ( t = 23.085, 24.187, 27.941, 15.722, 22.153, 13.277, 31.517, 32.901, 32.227, all P < 0.05) compared with the control group. The increases in white blood cell count and percentage of neutrophils in the second trimester group were significantly greater than those in the first and third trimester groups, and platelet count in the third trimester group was significantly lower than that in the first and second trimester groups (all P < 0.05). Conclusion:Monitoring routine blood indexes can help assess the health status of pregnant women. Adjusting nutrition program in time can decrease the incidence of adverse pregnancy outcomes.

Objective To study the influence of valsartan combined with indapamide on the UmAlb level and blood pressure in patients with hypertension and diabetes mellitus.Methods 90 patients with hypertension and diabetes mellitus were selected and randomly divided into three groups by number table.30 cases of the control group A were treated with indapamide,30 cases of the control group B were treated with valsartan,30 cases of the observa-tion group were treated with indapamide combined with valsartan.After treatment,the clinical parameters and effect of the three groups were compared.Results After treatment,the systolic blood pressure and diastolic blood pressure of the three groups were decreased(all P <0.05),and the observation group decreased obviously(all P <0.05).The total effective rate of the observation group was 96.7%,which was higher than 86.6% of the control group A and 83.3% of the control group B(χ2 =4.1 2,4.22,all P <0.05).Conclusion In the treatment of hypertension and diabetes,the antihypertensive effect of indapamide combined with valsartan is obvious and can protect the renal func-tion,which should be promoted in clinical use.

Objective To evaluate the effects of mechanical ventilation with heliox-oxygen on acute lung injury (ALI) in rabbits.Methods Thirty healthy New Zealand male rabbits, weighing 2.2-2.4 kg, were randomly divided into 3 groups (n =10 each) using a random number table: control group (C group), ALI group and mechanical ventilation with helium-oxygen group (HO group).The right common carotid artery was cannulated for fluid administration and blood sampling in the rabbits anesthetized with urethane.In ALI and HO groups, 1.2 ml/kg hydrochloric acid (HC1) of pH value 1.0 was injected into the right bronchus, followed by 2 ml air injection, and 5 min later 0.8 ml/kg HCI of pH value 1.0 was injected into the left bronchus, followed by 2 ml air injection.In C group, the equal volume of normal saline was given as previously described.After ALI, the rabbits were mechanically ventilated with airoxygen (air 50％-oxygen 50％) in C and ALI groups, or with helium-oxygen (helium 50％-oxygen 50％)in HO group.Mechanical ventilation (tidal volume 8 ml/kg, respiratory rate 30 breaths/min, inspiratory/ expiratory ratio 1 : 1) lasted for 4 h in the three groups.At 30 min before and after HC1 injection (T0,1) and 1, 2, 3 and 4 h of ventilation (T2-T5) , arterial blood samples were collected to detect the levels of surfactant-protein (SP-A) in serum by enzyme-linked immunosorbent assay.The animals were sacrificed at T5, and lungs were removed for examination of the pathologic changes with light microscope.The wet to dry lung weight ratio (W/D ratio) was calculated.Results Compared with group C, the levels of SP-A in serum were significantly increased at T1-T5, and W/D ratio was increased in ALI and HO groups.Compared with group ALI, the levels of SP-A in serum were significantly decreased at T5, and W/D ratio was decreased in HO group.Conclusion Mechanical ventilation with heliox-oxygen can reduce ALI in rabbits.

Objective:To observe the dynamic changes in serum gastroanteric hormones in patients with stroke complicating stress upper gastrointestinal hemorrhage in acute stage,the changes in acidity,and the changes in gastric empty and to investigate the pathogenetic link of stress pathological change of gastric mucous membrane in patients with stroke(Zhongzangfu,ZZF,中脏腑).Methods:In 20 cases with acute ZZF (Glasgow coma score 6-12 scores) who were all complicating upper gastroenteric bleeding within 24 hours of attack,the levels of motilin(MTL),somatostation(SS),and gastruins(GAS) were measured by radioimmunoassay at the first day (within 24 hours) and the fourth day (within 72-96 hours) respectively meanwhile the gastratube was plased and kept within 24 hours of attack to dynamically observe the pH of gastric juice and the condition of gastric emptying.Results:In comparison with control group the MTL level was significantly increased in patients at the first day of attack (P<0.05),the SS level significantly decreased (P<0.01),and the GSA level had not significant difference (P>0.05).Otherwise the pH (2.87±0.90） of gastric juice in comparison with control group has not significant difference (P>0.05).Among patients in 17 cases the gastric emptying time was prolonged.Even at the fourth day in patients the MTL level was still significantly increased (P<0.01),SS level decreased (P<0.05),and GAS level increased (P<0.01).Conclusions:The upper gastrointestinal hemorrhage in patients with acute ZZF is tightly associated with the changes in MTL and SS but the changes in GAS and gastric pH are not main factor.

Objective To observe the expression of matrix metalloproteinase-9 (MMP-9) and the regulation of neuregulin-1β (NRG-1β) in brain tissue in rats following cerebral ischemia/reperfusion injury. Methods The animal models of middle cerebral artery occlusion/reperfusion (MCAO/R) were established by a monofilament method from left external-internal carotid artery in 200 adult healthy male Wistar rats. The rat models in the treatment group (75 rats) and in control group (75 rats) were injected with 1.5%NRG-1β 5 μl and 0.1 mol/L PBS 5 μl, respectively, from internal carotid artery (ICA). The cerebral infarct volume was measured by TFC stain, the apoptosis was identified with in situ TUNEL method, and the expression of MMP-9 was detected by immunohistochemical and immunofluorescent double staining and Western blotting analysis. Results Cerebral ischemia-reperfusion can induce apoptosis and expression of MMP-9 in cerebral cortex and striatum. With the ischemic time prolonging, the number of apototic cells in cortex from ischemic 0, 0. 5, 1.0, 1.5 and 2. 0 h increased from 1.78 ± 0. 15,5. 78 ± 0. 51,10. 35 ± 0. 77, 21.50 ± 1.19 to 32. 00 ± 1.78, while the number of apoptotic cells in stratum from ischemic also increased significantly from 1.46±0.21, 4. 12±0.54, 7.33±0.71, 16.54 ± 1.63 to 19.03± 1.44 (t =9.31- 37.78, P < 0. 01) and the expression of MMP-9 increased significantly (t = 7.73-27.75, P < 0. 01) in the control group. With NRG-1β treatment, the number of apoptotie cells in cortex from ischemic 0, 0. 5,1.0, 1.5 and 2. 0 h reduced from 1.66±0. 11,4. 80±0. 61,5.63±0. 56, 9.75±1.22 to 13.54 ±1.26; while the number of apoptotic cells in striatum from ischemic also decreased significantly from 1.34 ± 0. 14, 3.35 ± 0. 32, 4. 55± 0. 50, 7. 63 ±1.41 to 10. 46 ± 0. 98 (t = 2. 74-18. 93, P < 0. 05), the expression of MMP-9 decreased (t = 3.85-12. 09, P < 0. 01), and the infarct volume decreased significantly (t = 4. 645-13. 043,P < 0. 01) compared with those in the control group at the same timepoint and the corresponding region. Conclusions The expression of MMP-9 is increased after cerebral ischemia/ reperfusion, and it may contribute to the inflammatory reaction. NRG-1β might down-regulate the expression of MMP-9 to inhibit apoptosis inducing by inflammatory reaction in cerebral ischemic reperfusion.

Objective: To investigate alveolar bone remodeling on both labial and lingual aspects of lower anterior teeth during treatment and retention stage. Methods: Forty-three cases with lower first premolars extracted (34 girls and 9 boys) and full records at three time points (pretreatment, posttreatment, follow-up) were collected as study sample. Displacement of lower incisor and bone thickness around its labial and lingual aspect were measured. Results:For the level of center of resistance(LC), there was statistically significant decrease of alveolar bone on lingual aspect (0.78?0.77) mm and no change on labial aspect, as the center of resistance(CR) was retracted lingually by (-3.02?1.13) mm during treatment. Analogous changes were found at the level of 3 mm apical to the center of resistance (L3C). In retention stage, with stable positioned lower incisor, no statistically significant change of alveolar bone was found on labial aspect at LC while a little amount of decrease of labial bone (0.20?0.58) mm was found at L3C. On the contrary, subsequential bone apposition could be detected with increase of lingual bone at both levels by (0.31?0.76) mm and (0.38?0.94)mm respectively. Conclusion:Speed of labial bone resorption is faster than that of lingual bone apposition during orthodontic treatment. In retention stage, sequential bone apposition on lingual aspect is presented with lower incisor in stable position.