Objective:To investigate the clinical characteristics of urinary system abnormalities in Chongqing and its surrounding area, and to assess the role of the large-scale urinary system ultrasound screening in the asymptomatic children with kidney and urinary tract abnormalities. Methods:We performed urinary system ultrasound on Screening 14 256 children from September 2008 to October 2008 in Chongqing Children’s Hospital. The total samples were allocated into five groups as follows:less than 1 year group;1 to 3 years group;3 to 5 years group;5 to 10 years group ;more than 10 years group. Then according to each group’s age,sex,disease location,severity difference,we performed statistical analysis. Results:409(2.87%)of 14 256 children had abnormalities,284 of whom was diagnosed with significant anomalies,including hydronephrosis,renal maldevepment (aplastic,dysplastic,hypoplastic,ectopic kidneys)and renal agensis,nephrolithiasis,simple renal cyst,multicystic dysplastic kidneys (MCKD),ureter dilation,duplex kidneys and so on. Among these anomalies,the most prevalent abnormal finding was hydronephrosis accounting for 130 cases,almost a half of 284 (45.77%),92 cases in males,38 cases in femal. The prevalence of melamine related urinary calculus was as 5.17 times as that of others and its 95% confidence interval(CI)of population relative risk was [3.28,8.14]. Conclusion:① Ultrasonography in the diagnosis of diseases of the urinary system can play an important role,especially for congenial anomalies of kidney and urinary tract(CAKUT)and /or hydronephrosis and so on. Therefore,an early diagnosis of these defects allows to realize as fast as possible the best medical and/or surgical treatment, preventing or at least slowing down the progression to chronic renal failure. ② The prevalence rate of hydronephrosis in male is higher than female. ③Exposure to melamine- contaminated formula is associated with urinary stones.

Objective: To study effects of tertiary rehabilitation training on upper limb motor function recovery in patients with cerebral hemorrhage.Methods: A total of 120 patients with cerebral hemorrhage, who were treated in our hospital from May 2013 to May 2015, were selected.According to random number table, they were randomly and equally divided into routine rehabilitation group and tertiary rehabilitation group, and both groups received the same routine medical treatment and nursing.Simple test for evaluating hand function scale (STEF), Fugal-Meyer motor assessment (FMA) and Brunnstrom limb motor function assessment were used to assess and compare limb recovery after rehabilitation training between two groups.Results: At the end of first month of rehabilitation training, there were no significant difference in STEF and FMA scores between two groups, P>0.05 both.On the end of second month of rehabilitation training and later, compared with routine rehabilitation group, there were significant rise in STEF score[end of second month: (3.3±0.5) scores vs.(4.2±1.1) scores, end of sixth month: (6.3±1.2) scores vs.(8.5±1.1) scores], total STEF score[(20.2±4.2) scores vs.(38.2±3.5) scores]and FMA score[end of second month: (28.4±3.6) scores vs.(33.4±2.7) scores, end of sixth month: (38.2±6.6) scores vs.(46.3±5.8) scores]in tertiary rehabilitation group, P<0.05 all.Compared with routine rehabilitation group after six-month rehabilitation training, there were significant rise in percentages of Brunnstrom stage V (23.33% vs.43.33%) and stage VI (6.67% vs.20.00%) in tertiary rehabilitation group(P<0.05 both).Conclusion: Tertiary rehabilitation training can significantly improve upper limb motor function in patients with cerebral hemorrhage, so it's worth extending.

Objective: To evaluate the effect of dietary fiber and phytic acid inositol hexaphosphate (IP6 or Insp6) on 1,2-dimethylhydrazine(DMH) induced colorectal carcinogenesis in Wistar rats. Methods: 86 four-week-old male Wistar rats were divided randomly into 6 groups and fed with either basal fiber free diet or the basal diet supplemented with 10% pectin, 10% cellulose, 2% Na-InsP6 supplemented water, 10% pectin in combination with 2% Na-InsP6 supplemented water, 10% cellulose in combination with 2% Na-InsP6 supplemented water. After four weeks, the rats were given a weekly injection of DMH (20mg/kg bw) for 20 weeks. The rats were killed after 26 weeks. The number of total macroscopically visible neoplasms was counted and the volume of individual neoplasmas was calculated. Proliferation cell nuclear antigen (PCNA) was analyzed. Results: There was no significant difference between the treatment group and the control group in the incidence of tumors. InsP6 significantly reduced the number of tumors in rats and tumor volume. Pectin and Pectin+InsP6 significantly increased the number of tumors in rats. The expression of proliferation marker PCNA was significantly down-regulated by InsP6 and significantly increased by pectin. Conclusion: A treatment regimen of 2% Na-InsP6 in drinking water was effective in significantly reducing the risk of colorectal cancer in Wistar rats. Diet supplemented with pectin could increase the risk of colorectal cancer.

Objective To approach the involved position and regulation as well as the clinical characteristics of hereditary multiple exostoses(HME).Methods One HME pedigrees including men and women 7 cases respectively confirmed clinically and pathologically was analysed.Results The most common location of exostoses was metaphysis of long bones.No exostoses was found in craniofacial bone.Conclusion There is no sex difference in the incidence of HME and the location of exostoses is mostly at metaphysis of long bones in this family.

BACKGROUND:Traditional Chinese medicine shares wide application in clinical treatment because of its high security. At present, a lot of studies have been reported, in which, traditional Chinese medicine or monomers are used for in vitro differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells.
OBJECTIVE:To summarize and analyze the research progress of Chinese medicine to induce the differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells and to looking prospect for its application.
METHODS:CNKI database was searched for the articles about Chinese medicine-induced differentiation of bone marrow mesenchymal stem cells into cardiomyocytes, published from January 2001 to January 2013. The key words were“Chinese medicine, bone marrow mesenchymal stem cells, cardiomyocyte”in Chinese and“bone marrow mesenchymal stem cells, myocardial cells”in English. Obsolete or repetitive articles were excluded. Final y, 36 articles were included in result analysis.
RESULTS AND CONCLUSION:Different Chinese medicine formulations were used as inducers to induce differentiation of bone marrow mesenchymal stem cells into cardiomyocytes, including Panax notoginseng saponins, salvianolic acid B, Jiawei Danshen Yin, icari n, astragaloside. Then, the induced cells were examined by immunohistochemistry and reverse transcription-PCR. The results showed that the induced cells were positive for Desmin, cardiac troponin I and major histocompatibility complex. Positive cells were fusiform and fibroblast-like morphology, which indicates a role in promoting proliferation and differentiation. These findings show that Chinese medicine-induced differentiation of bone marrow mesenchymal stem cells into cardiomyocytes has become a worldwide research hotspot, providing a theoretical basis for clinical treatment of ischemic heart diseases.

Objective To explore the clinical effects of injecting methotrexate with hysteroscopic tubal can-nulation combined with Huayu shapei prescription in the treatment of salpingocyesis.Methods 82 unruptured salpin-gocyesis patients were randomly divided into the two groups.41 patients in the observation group were treated by hyst-eroscopic tubal cannulation injection of methotrexate combined with Huayu shapei prescription,41 patients in the con-trol group were treated by methotrexate intramuscular injection with oral mifepristone.The clinical efficacy of the two groups were observed,the level of serumβHCG required for tubal pregnancy,the mass absorption and after treatment of tubal patency.Results Compared with the control group,the difference was significant of the decline of serum βHCG in the treatment group (t=2.67,P<0.05).Compared with the control group,the treatment effectiveness and the absorption of the mass in fallopian tube in the treatment group,the difference was significant (t =3.57,P<0.05 ).Tubal patency detected treated group was superior compared with the control group,the difference was signifi-cant(χ2 =100.23,P<0.05).Conclusion Compared with traditional treatments,injecting methotrexate with hyste-roscopic tubal cannulation combined with Huayu shapei prescription in the treatment of salpingocyesis can effectively reduce the HCG levels,promote the absorption and softening of the ectopic pregnancy mass,to achieve the purpose of killing embryos eliminate micro,it will help to improve patients with clinical symptoms and signs,to maximize the recovery of the patient's reproductive function and physical health.

Objective To investigate the involvement of apoptosis in the lesions of patients with psoriasis. Methods The apoptosis was detected with terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL), and the expression of p53, PCNA and apoptosis suppressing protein bcl 2 was assessed with immunoperoxidase technique in psoriatic lesions and normal skin. Results A large number of keratinocytes showing biochemical and morphologic features of cells undergoing apoptosis were observed in all the suprabasal layers of the psoriatic epidermis. The plaques from all patients analysed showed marked increase in the number of PCNA positive cells in the middle and basal keratinocytes, and a dramatic reduction in the number of bcl 2 positive cells in the basal cell layer. Conclusion The increased apoptosis of keratinocytes in the lesions of psoriasis might be a homeostatic mechanism to the hyperplasia of cells.

BACKGROUND: Physical training induces adaptation changes on physiological function and anatomic structure of body, which significantly exist in individuals. Individual variation responses to physical training is determined by gene polymorphism. OBJECTIVE: To summarize the research progress on the association between gene polymorphism and exercise training sensitivity in recent years. RETRIEVAL STRATEGY: A computer-based search of Pubmed database was undertaken to identify the articles published from January 2002 to December 2006 with the key words of "gene polymorphism, endurance training, strength training, aerobic training" in English. Simultaneously, China Journal Full-text Database was searched for the relevant articles published from January 2002 to December 2006 with the same key words in Chinese. Totally 58 relevant articles were collected after the first trial. Inclusive criteria: ①articles on association between gene polymorphism and endurance training, ②articles on association between gene polymorphism and strength training, ③articles on association between gene polymorphism and hypoxia training. LITERATURE EVALUATION: The articles collected were prospective cohort studies on association of gene polymorphism with some physiological and biochemical parameters response to physical training. DATA SYNTHESIS: ①The individual variation responded to physical training in physiological and biochemical parameters are mostly determined by genetic factors, such as gene polymorphism.②Recently, there are lots of studies on association of gene polymorphism with physiological and biochemical parameters response to endurance training, strength training and hypoxia training.③Mostly studies indicate that there are associations among physiological and biochemical parameters response to endurance training, strength training and hypoxia training and I/D polymorphism in ACE gene, C1772T in HIF-1? gene and so on. CONCLUSION: Although gene polymorphism is a genetic marker of determining the physiological phenotype of human, the various genetype of the same gene polymorphism can cause different sensitivity change of physiological phenotype in the different races response to training. With the research progress on association between gene polymorphism and physical training sensitivity, it will indicate its related mechanism.

BACKGROUND: Incidence rate of carcinoma of large intestine increases with the development of national living standard. Many epidemiologic surveys have showed that dietary factors have closely correlation with high risk of carcinoma of large intestine.OBJECTIVE: To observe the effect of dietary fiber and inositol hexaphosphate on the incidence of carcinoma of large intestine by establishing rat models with carcinoma of large intestine.DESIGN: Randomized block design.SETTING: Institute of Nutriology, Medical College, Qingdao University.MATERIALS: The experiment was performed at the Institute of Nutriology, Medical College, Qingdao University from March to December 2004.Totally 86 male Wistar rats of 4 weeks were randomly assigned based on body mass into cellulose group with 14 rats, pectin group with 14 rats, inositol hexaphosphate group with 15 rats, cellulose plus inositol hexaphosphate group with 14 rats, pectin plus inositol hexaphosphate group with 14rats and control group with 15 rats.METHODS: Basic feed without dietary fiber was given in the control group. 10% pectin was added in the pectin group. 10% cellulose was added in the cellulose group. 2% sodium phytate was added in the water of inositol hexaphosphate group. 10% pectin and 2% sodium phytate were added in the water of pectin plus inositol hexaphosphate group. 10% cellulose and 2% sodium phytate drinking water were added in the cellulose plus inositol hexaphosphate group. A total of 86 rats received 1,2-dimethylhydrazine (DMH) by subcutaneous injection to induce carcinoma of large intestine at week 4 after breeding. Incidence rate of large bowel neoplasm,number and volume of tumor were observed. Proliferating activity of large intestinal mucosa cells was determined (the ratio of number of positive cells of proliferating cell nuclear antigen to total count of nucleus).MAIN OUTCOME MEASURES: ①Incidence rate of large bowel neoplasm in each group, changes of mean number and mean volume of tumors of each rat, and ②proliferating activity of large intestinal mucosa cells of rats.RESULTS: ①Most of the rats in each group died during the 20-week trial. Oue rat died in the pectin group, the pectin plus inositol hexaphosphate group and the control group, respectively after 20 weeks. ②There was no significant difference in the incidence rate of large bowel neoplasm of rats compared with the control group (P ＞ 0.05), but the mean number and mean volume of tumors of each rat in the inositol hexaphosphate group were significantly lower than those in the control group [quantity: (1.1±0.2),(4.1±1.2)rats,P ＜ 0.01 ;volume: (176.1±65.5), (1 046.7±469.0)mm3,P ＜ 0.05], and the mean number of tumors of each rat in the pectin plus inositol hexaphosphate group was obviously higher than that in the control group [(7.5±1.9), (7.2±1.0) rats,P ＜ 0.05]. ③The proliferating activity of large intestinal mucosa cells in the inositol hexaphosphate group significantly decreased as compared with the control group. The proliferating activity of large intestinal mucosa cells in the pectin group increased as compared with the control group [(41.8±4.7)%, (83.6±2.9)%,(66.7±7.8)% ,P ＜ 0.01 and 0.05].CONCLUSION: It may increase the incidence rate of carcinoma of large intestine when pectin is added in diet, on the contrary, the rate decreases if inositol hexaphophate is added in the water.

Objective To analyze the blood screening results after adjustment of critical value 40 to 50 U/L and to observe the effect of reducing blood scrap rate and to discuss the correlation between ALT and HBV,HCV infection.Methods We screened 2656 blood donors (ALT >40 U/L)by serological and nucleic acid amplification testing(NAT)in Qingdao blood center from 2013 to 2014,and conducted the correlation analysis by chi square test.Results 1 771 cases (66.68%)were ALT 40-50 U/L,including 6 cases of HBsAg ELISA (+),2 cases NAT (+),4 cases NAT(-).In the 8 cases of anti-HCV ELISA (+)samples,4 cases NAT (+),3 cases NAT (-),1 case with positive TP without NAT result.In 885 blood donors with ALT>50 U/L,5 cases were HBsAg-reactive,7 cases were anti-HCV-reactive,and 873 cases were negative.Related statistics showed that there was no signifi-cant difference between ALT and HBV infection (P <0.05),but significant difference was found between ALT and HCV infection (P >0.05).Conclusion The proportion of blood donors with ALT 40-50 U/L is much higher than that with ALT >50 U/L do-nors.Adjustment of the critical value greatly reduces blood scrap rate.Elevated ALT is associated with the infection of HBV but not with HCV.