This paper discusses the significance of situation and policy education of medical students and actively explores how to strengthen the education.

Since the innovation thought was proposed in the 1950s,the institutions of higher education of various countries all attach importance to the cultivation of students,innovation ability,which is the need of the time and the requirements of quality education as well.Therefore,it is necessary to create democratic and free environment,conducive to innovative thinking in teaching activities and positive organizational innovation practice,raises university students innovation thought,and improve the university student quality.

In light of the objective circumstances and its subjective conditions, our hospital, a grade A, second tier urban general hospital, made the right choice of reorienting itself towards community health service after making a careful assessment of the current situation. In the course of the reorientation, our hospital mainly focused on such tasks as change of concepts, readjustment of its structure and functions, establishment of the service mode of the family physician, delivery of the "six in one" service in the form of a team, and implementation of standard management. Such efforts proved to be fruitful and won the approval of the community. On the basis of this, our hospital tried hard to exploit to the full its favorable conditions as a second tier hospital in providing community health service and called for attention and solution to such issues as the pricing system, the fundraising and compensation mechanism, cost accounting, and reform of the personnel system in the delivery of community health service.

Objective To investigate the clinical value of anti-nuclear antibody(ANA) and anti-nuclear antibody spectrum(ANAs) detection.Methods A total of 2 325 patients with or suspected with autoimmune diseases(AID) were enrolled and detected for ANA and ANAs by using indirect immunofluorescence assay(IIF) and linear immunoblot assay(LIA) respectively.All detected results were analyzed.Results Among 2 325 patients,896 cases(38.54%) were positive with ANA,with positive rate of 45.46% in female patients,which was higher than the 18.46% of male patients(P<0.05),and the common fluorescence patterns were nuclear particle pattern,nuclear homogeneous pattern and the nucleolus pattern.816 cases(35.10%) were positive with ANAs,and the positive rates of anti-Sjogren's syndrome(SS)-B antibody,anti Ro-52 antibody and anti SS-A antibody were relatively higher.The consistency rate of the two methods was 91.66%.Conclusion ANA and ANAs detection could be with certain correlation,but might be not completely consistent,detection could improve the detection rate and reduce the missed detection rate.

Immune response is one of the main reasons causing neurological deficits in the patients with cere -brovascular diseases , which activates microglia , induces inflammatory reaction and finally results in serious neuronal and endothelial injury .MicroRNAs take part in the regulation of immunoreaction , and simultaneously regulates many target genes and induces faster post-transcriptional regulation to its target genes compared with the traditional transcriptional regu -lation.For providing a basis for the clinical use of microRNAs and applying new therapy , this review mainly focuses on the function and mechanism of microRNAs in the regulation of the immunoreaction caused by cerebrovascular diseases .

Life education is the education based on perception and experience of life,the very nature of which is the cultivation of life awareness.It is the urgent requirement to carry out life education for university students,because it helps them to adjust to the developing and changing society and promotes their physical and psychological development coordinated.In order to strengthen college students' rational thinking on the value and significance of life,enhance their humanistic quality,life education should be incorporated into higher education system independently.

With great theoretical and practical significance,training demand analysis is the prerequisite and basis for other training activities.This paper gives a brief introduction and analysis to modern training demand which featuring with diversity and multi-hierarchy.This paper analyzes the participants' needs and identifies the influence factors by using chi-square test and other methods to meet the needs of training programs.And we provide the detailed solutions for addressing those problems.

Objective To establish a new cell resource of stem cell transplantation for heart failure therapy,by using cardiomyocytes from rat marrow mesenchymal stem cells(MSCs). Methods MSCs were isolated and cultured from rat bone marrow,then induced by 5aza(5,10,15,20 ?mol/L) for 24 hours.The cultured cells were observed with phase-contrast microscopy.The immunohistochemical technique and laser scanning confocal microscopy(LSCM) were applied for revealing the expressions of desmin,?-sarcomeric actin and C-TnT.The induced cells were evaluated by a transmission electron microscope.GATA4 and(?-MHC) expressions were detected by relative quantitative RT-PCR after 7,21,28 days of inducement respectively. Results MSCs induced in vitro by 5-aza could be identified by the positive staining for desmin,?-sarcomeric actin and C-TnT.The number of cells stained positively in the 5 ?mol/L was greater than that of other groups.Transmission electron microscope showed that paralleled myofilaments were formed.RT-PCR assessment showed that the differentiated cells began to express GATA4 from day 7 to day 28 of differentiation and began to express ?MHC from day 21 to day 28 of differentiation.Conclusion Cardiomyocyte-like cells can be generated from MSCs in vitro and the marrow mesenchymal stem cells are one of the best resources of donor cardiomyocytes.

Strengthening the capacity of medical students' clinical thinking is an important aspect of medical education,the writers believe that medical students' clinical thinking ability can be improved through three aspects which include basic teaching practice,thinking knowledge based learning and clinical teaching.

The soft environment is the subsystem of the university moral education environment system,including the university spirit,academia environment,human environment, system environment and network environment. The soft environment construction has the important value in the university moral education,which not only provides the base for the university students to develop morals amd creates their intrinsic demand for morals but also affects the university moral education activities. It is the objective foundation for carrying out such activities and affects the decision-making and the effect of activities.