Objective To investigate the protection against podocyte injury by simvastatin in DM rats. Methods We divided DM rats induced by STZ into 3 groups of NC,DM and SVT,measured Scr,TC and UAER，observed renal pathomorphology and FPW，and detected nephrin,podocin,NF-κB and MCP-1 by immunohistochemistry stain,real time-PCR. Results The levels of Scr,UAER,NF-κB and MCP-1 were higher in DM than in NC and SVT group.The level of FPW was higher in DM than in SVT group.The levels of nephrin and podocin were lower in DM than in NC and SVT group (all P<0.01). Conclusions Simvastatin can suppress inflammatory cytokines,maintain podocyte protein,relieve podocyte injury.

MiRs display an important role in a variety of biological, physiological and pathological processes, including cell proliferation, differentiation, apoptosis, individual development, occurrence and progress of diseases. Recent studies have discovered that miR?424 is the significant regulatory factor of angiogenesis, and is involved in many diseases such as infectious diseases, vascular diseases, central nervous system diseases and genital system disease. This article reviews the expression, effect and possible mechanisms of miR?424 in non?tumorous diseases.

Objective To observe the effect of different doses of fat emulsion on blood lipid and liver function in critically ill premature infants. Methods 40 babies were randomly divided into 4 groups,receiving fat emulsion 0,1,2,3 g?kg~(-1)?d~(-1) for 5 days respectively. The blood levels of cholesterol and triglyceride and the serum biochemical indexes relative to liver functions were measured before and after the period of 5 days. Results Blood lipid and serum ?-GT levels did not change remarkably in 1 or 2 g?kg~(-1)?d~(-1) group, but increased significantly in 3 g?kg~(-1)?d~(-1) group. Serum bilirubin levels decreased in all groups, but it was significantly higher in the group of 3 g?kg~(-1) ?d~(-1) than the others. Conclusion It is suggested that the blood lipid level would increase with 3g?kg~(-1)?d~(-1) of fat emulsion used in critically ill premature infants and that the use of fat emulsion would retard the falling of serum bilirubin level when used in newborn infants with byperbilirubinemia.

Objective: To investigate alveolar bone remodeling on both labial and lingual aspects of lower anterior teeth during treatment and retention stage. Methods: Forty-three cases with lower first premolars extracted (34 girls and 9 boys) and full records at three time points (pretreatment, posttreatment, follow-up) were collected as study sample. Displacement of lower incisor and bone thickness around its labial and lingual aspect were measured. Results:For the level of center of resistance(LC), there was statistically significant decrease of alveolar bone on lingual aspect (0.78?0.77) mm and no change on labial aspect, as the center of resistance(CR) was retracted lingually by (-3.02?1.13) mm during treatment. Analogous changes were found at the level of 3 mm apical to the center of resistance (L3C). In retention stage, with stable positioned lower incisor, no statistically significant change of alveolar bone was found on labial aspect at LC while a little amount of decrease of labial bone (0.20?0.58) mm was found at L3C. On the contrary, subsequential bone apposition could be detected with increase of lingual bone at both levels by (0.31?0.76) mm and (0.38?0.94)mm respectively. Conclusion:Speed of labial bone resorption is faster than that of lingual bone apposition during orthodontic treatment. In retention stage, sequential bone apposition on lingual aspect is presented with lower incisor in stable position.

A member of miR-16 family, miR-424 has been found to be closely related with tumorigenesis, tumor progrssion, prognosis and therapy.This article reviews the expression changes, roles and possible regulating mechanisms of miR-424 in leukemia and various tumors such as breast, cervical, lung, liver and colorectal cancers.Recent studies have demonstrated that the expression of miR-424 is affected by many factors, and miR-424 could be a biomarker of diagnosis, staging and prognosis in cancers,to identify the area of tumor, and be a target of therapy.

Objective To analyze MRI features and diagnostic value in adenomyosis.Methods Clinical and MRI data of 31 cases of adenomyosis confirmed by pathology were retrospectively analyzed.Turbo FSE T2WI,SE T1WI,and sagittal Turbo FSE T2WI examination were performed in all cases,10 of them were added to anatomic and sagittal fat-suppression(FS) T2WI scan.MR features of adenomyosis were analysed.Results In 19 cases of diffuse uterine adenomyosis,11 cases mainly showed diffusely thichened junctional zone of posterior wall.12 cases were focal uterine adenomyosis,the lesions located at posterior wall in 7 cases,front wall in 4 cases and bottom part in 1 case.28 cases displayed hypointensive masses like the intensity of junctional zone on T2WI and vesicular hyperintensive foci were found within the hypointensive lesions.The lesions in 21 cases were similar to that of myometrium on T1WI,9 of them displayed vesicular hyperintensive foci.Focal round-like junctional zone low signal intensity lesion in myometrial layer was found in 3 cases.Conclusion MRI can clearly determine the size and site of adenomyosis.FS is the valuable examination way.

Objective To investigate the apoptosis rate of neurons in vitro among the primary culture cells isolated from rat spinal cords before and after injury. Methods The spinal cord neurons of Wistar rats at 14-day gestation were isolated and cultured. The neuronal processes were then injured by cutting. At different time points after injury, TUNEL method was employed to detect the apoptotic neurons. Results Before injury, there was almost no apoptotic neuron. However, a large amount of apoptotic neurons were observed after the injury. The highest incidence of apoptosis appeared on the first day, and then it gradually reduced in the following days, but increased in amount again on the seventh day. Conclusion The results of the present experiment reveal the regularity of apoptosis of neurons before and after injury, and it provides a platform for further research regarding therapeutic intervention in the treatment of spinal cord injury.

Objective To establish a model of injury to primarily cultured spinal cord neurons,mimicking the neuronal injury after complete transactional spinal cord trauma,for the sake of exploring changes in expression of an immediately-early gene,c-jun,in central nervous system injury. Methods Spinal cords were removed form fetal Wistar rats at the 14th gestation day and the neurons were cultured for 10 to 12 days.Then,mechanical injury was applied to the neurons by making regular scores on the culture disk under direct vision with the aid of a self-made standard template.Morphology of the injured neurons and changes in expression of c-Jun protein were observed before and at different intervals after injury. Results c-Jun expression was noted in neuronal nuclei 10 min after injury and its peak appeared at 2 hrs.Besides,the density of positive neurons bore evidently an inverse proportion with their distance from the scores.Conclusion\ Positive expression in injury neurons show that c-jun gene enters the nucleoli of injured neurons and takes the role of “the third messenger” at early time after neuronal injury.

Objective To explore the protective effect of HGF on primary cultured spinal neurons in vitro.Methods Cultured neurons were transfected with Ad-GFP or Ad-HGF in different multiplicity of infection(MOI),then flow cytometer and PI-Hoechst double stains were used to assay transfer rate or to determine the status of cells respectively.ELISA was used to detect the expression of HGF in Ad-HGF transfected neurons,and the activity of neurons was judged by neutral red stain,MTT and NSE-ELISA methods.Results After transfection with Ad-GFP in MOI of 50,the transfer rate was high as detected by flow cytometer,and the status of cells was well as judged by PI-Hoechst double stain.The results of ELISA showed that HGF was expressed in medium.After transfection with Ad-HGF,the activity of neurons was better than neurons without treatment(P

BACKGROUND: Hepatocyte growth factor (HGF) promotes neurite outgrowth from neocortical explants, and supports neuronal survival under serum-free condition. Thus, HGF can mediate neurotrophic function as a novel neurotrophic factor.OBJECTIVE: To establish an in vitro injury model with a semi-solid culture system for the purpose of improving the evaluation of neurite regeneration of transected spinal cord neurons from rat embryo, and investigate the effect of HGF on neurite regeneration.DESIGN: Randomized controlled study.SETTING: Hematology Laboratory of Radian Medical Institute of Academy of Military Medical Sciences of PLA.MATERIALS: The experiment was carried out at the Hematology Laboratory of Radian Medical Institute of Academy of Military Medical Sciences of PLA from August 2004 to May 2005. Wistar fetal rats of 14-16 days old were provided by Animal Center of Academy of Military Medical Sciences of PLA. Tail collagen was extracted from adult male Wistar rats with body mass of (250±50) g.METHODS: ① Rat tail type Ⅰ collagen substrate was prepared and spread on a culture dish, cut into about 0.5-1.0 mm3 slices, then spinal cord slices of 15-day-old fetal Wistar rat were explanted on the primary culture. Five days later, the outgrowing processes were severed, then a block of collagen, with the surface area of 2 mm2 and 200 μm away from the slice, was removed and the vacancy was replaced with a fresh collagen block of 2 μL after aspirating the medium. The fresh collagen block could be solidified and then fresh liquid medium was added as the secondary culture. The regeneration of neurite was observed by microscopy at 0, 1, 6,12 and 24 hours after severing. ② The medium was changed with 0.5% N3-conditioned medium. 10 μg/L HGF was added in the experimental group, and 0.5% N3-conditioned medium was added in the control group.The status of regeneration was evaluated by the average value of 3 longest regenerative neurites for each slice. There were 12 slices in each group.The status of neurite regeneration was calculated and was evaluated 24 hours later.MAIN OUTCOME MEASURES: ① neurite regeneration in situ; ②comparisons of neurite regeneration between control group and experimental group.RESULTS: ① Neurite regeneration in situ: The neurites disintegrated near the severing line immediately following the transection injury. This process persisted about 1-2 hours and the distance away from the severing line was about 20 μm. Then the proximal end of neurites would swell and thicken. At this time neurites stopped collapsing and neurite regeneration began. Their regenerating rate would quicken at 12 hours after severing. Regenerating neurites were more branching and curlier as compared with original neurites. ② Comparisons of neurite regeneration between control group and experimental group: The average length of regenerative neurites was more in the experimental group than that in the control group [(375±96) μm, (200±75) μm, P ＜ 0.05].CONCLUSION: ① We establish a simple, economic model to evaluate neurite regeneration. ② By this model, we prove that HGF can promote neurite regeneration.