Objective To investigate the sensitivity and specificity of different methods for the detection of mycoplasma pneu‐moniae .Methods The serum and throat swab specimens were collected from 199 pneumonia patients .ELISA and real‐time fluores‐cence quantitative polymerase chain reaction (FQ‐PCR) assays were used to detect the serum mycoplasma pneumoniae IgM antibod‐y and DNA .Results Among 199 pneumonia patients ,61 cases were diagnosed as mycoplasma pneumoniae pneumonia according to the diagnosis standard .The sensitivity and specificity of ELISA and FQ‐PCR for detecting mycoplasma pneumoniae were 86 .9%and 96 .7% ,and 78 .3% and 97 .1% ,respectively .Conclusion The sensitivity and specificity of FQ‐PCR for detecting mycoplasma pneumoniae are higher than those of ELISA .

Objective: To study effects of tertiary rehabilitation training on upper limb motor function recovery in patients with cerebral hemorrhage.Methods: A total of 120 patients with cerebral hemorrhage, who were treated in our hospital from May 2013 to May 2015, were selected.According to random number table, they were randomly and equally divided into routine rehabilitation group and tertiary rehabilitation group, and both groups received the same routine medical treatment and nursing.Simple test for evaluating hand function scale (STEF), Fugal-Meyer motor assessment (FMA) and Brunnstrom limb motor function assessment were used to assess and compare limb recovery after rehabilitation training between two groups.Results: At the end of first month of rehabilitation training, there were no significant difference in STEF and FMA scores between two groups, P>0.05 both.On the end of second month of rehabilitation training and later, compared with routine rehabilitation group, there were significant rise in STEF score[end of second month: (3.3±0.5) scores vs.(4.2±1.1) scores, end of sixth month: (6.3±1.2) scores vs.(8.5±1.1) scores], total STEF score[(20.2±4.2) scores vs.(38.2±3.5) scores]and FMA score[end of second month: (28.4±3.6) scores vs.(33.4±2.7) scores, end of sixth month: (38.2±6.6) scores vs.(46.3±5.8) scores]in tertiary rehabilitation group, P<0.05 all.Compared with routine rehabilitation group after six-month rehabilitation training, there were significant rise in percentages of Brunnstrom stage V (23.33% vs.43.33%) and stage VI (6.67% vs.20.00%) in tertiary rehabilitation group(P<0.05 both).Conclusion: Tertiary rehabilitation training can significantly improve upper limb motor function in patients with cerebral hemorrhage, so it's worth extending.

Objective To assess clinic value of esophageal stent placement in the benign and malignant esophageal strictures,and the influence of stents selection on curative effect. Method 36 cases of esophageal strictures, 5 cases of benign strictures,31cases of malignant strictures,including 4 cases with esophagotracheal fistula and 3 cases with esophagomediastrinal fistula. 26 domestic Nickel-Titanium alloy memorial netlike stents and 14 membrane-covered stents were used for the treatment. All patients were treated by placing esophageal stent perorally under fluoroscopic guidance. Results 40 stents were planced successfully in 36 cases, only one case showed membrane covered stent migrating. Symptoms of dysphagia were considerably relieved after stent insertion. The total effective rate was 97.2%. Conclusion The esophageal stent is an effective and safe treatment for benign and malignant esophageal stenosis: The nutritive condition and the quality of life of patients are improved, which is favourable for tumor further treatment. Appropriate stent selection can keep esophageal patent rate and decrease sliding rate.

Objective To observe the clinical value and the effect of interventional treatment on obstructive jaundice. Methods 24 patients with obstructive jaundice, including 21 cases of malignant biliary obstruction and 3 cases of chololithiasis post operative biliary stricture of bile duct, were treated after percutaneous transhepatic biliary moulding, 6 cases with percutaneous transhepatic cholangic drainage(PTCD), 18 cases with insertion of biliary stents, 13 net-type stents and 5 Z-type stents were used, according to the patient's state and financial condition. Results The total technical success rate was 100%. After 7～15 days of interventional treatment, the total serum bilirubin level declined from (454 93?64 52)?mol/L to (66 94?8 45)?mol/L, transaminase declined from (265 67?30 09)u/L to (56 21?8 73)u/L. There was very significantly difference (P

OBJECTIVE To investigate the incidence,symtoms and prognosis of hospital infection by Mucor after liver transplantation.METHODS The clinical data of all the patients with complicated hospital infection orthotopic by Mucor after orthotopic liver transplantation were analyzed restrospectively.RESULTS There were 9 cases with complicated hospital infection by Mucor from in 750 liver transplant recipients.Seven cases developed superficial incisional infection with delayed healing and rapid increased secretion on the surface of incisions.All of them recovered after antifungal therapy.Two cases had pulmonary Mucor infection combined with bacteria and died of multi-organ failure.CONCLUSIONS The incidence of hospital infection by Mucor in liver transplant recipients is about 1%.The most common symtoms are superficial incisional infection and the patients recover well.However,the cases with complicated pulmonary Mucor infection are difficult to treat and have a poor prognosis.

Objective To investigate the effects of dexamethasone on the expression of glucocorticoid receptor (GR ?, GR ?) in human monocyte cell line THP-1.Methods Cultured THP-1 cells were stimulated by dexamethasone with different concentrations and for different durations. The GR ? and GR ? protein expressions were examined by Western blotting. Results The expressions of GR ? and GR ? were observed in stimulated and unstimulated THP-1. The quantity of GR ? expression was reduced by dexamethasone treatment in a time-dependent manner. The quantity of GR ? expression was increased by dexamethasone treatment in time- and dose-dependent manner. Conclusion Dexamethas one stimulation time-dependent reduce GR ? expression in THP-1 cell. Dexamethasone stimulation time- dependent and dose-dependent increase GR ? expression in THP-I. The expressions of GR a and GR ? were regulated by glucocorticoid.

MicroRNA-494 (miR-494) is one of the microRNAs from 14q32.31 miR-gene cluster.Recently, miR-494 was found to closely relate with tumors and other diseases .This article reviews the expression changes , roles and possible regulatory mechanisms of miR-494 in multiple tumors and other hypoxia/ischemia diseases .Recent studies demon-strate that the expression of miR-494 is affected by many factors , and miR-494 could be a biomarker of diagnosis , staging and prognosis in tumors and other diseases , and a target of therapy in future .

Background and purpose: Docetaxel/cisplatin are widely used in chemo-naive patients with advanced non-small cell lung cancer(NSCLC),but the standard 3-weeks'project of docetaxel caused significant toxicity.We performed this study to compare the effect and toxicity of modified and standard 3-weeks'docetaxel/cisplatin as first line chemotherapy for advanced NSCLC.Methods:68 patients with stage ⅢB or Ⅳ NSCLC(proven by histology or cytology) were randomly divided into two groups,modified(A) and standard(B) chemotherapy.Group A: docetaxel 75 mg/m2,divided into 2 days,ivgtt d1 and d 8,cisplatin 25 mg/(m2?d),ivgtt d 1-d 3,q3w;Group B: docetaxel 75 mg/m2,ivgtt d 1,cisplatin was administered as Group A,q3w.The effect and toxicity were assessed after two cycles and one-year survival was followed up.Results:There was no CR in both groups.10 PR,20 SD,4 PD were found in group A,the overall response rate is 29%;whereas 11 PR,20 SD,3 PD were found in group B,the overall response rate is 32%.The one-year survival rate were 38% and 35% in group A and B,respectively.There were no significant difference about the overall response rate(P=0.793) and one-year survival rate(P=0.801) between group A and B.The rates of grade Ⅲ/Ⅳ neutropenia were 18% and 47% in group A and B respectively.The difference was statistically significant(P=0.010).Conclusions:In comparison with the standard 3-weeks'docetaxel/ cisplatin chemotherapy,the modified one has similar response rate but lower hematologic toxicity,and thus it was well tolerated.

Objective To study the change of intestinal permeability in patients with acute pancreatitis (AP) to investigate the indication of early operation in AP patients. Methods Serum concentrations of glutamine, endotoxin and urine lactulose/mannito (L/M) ratio were measured and compared at different time in 18 AP patients received non-operative treatment and 7 underwent operation . Results Serum concentrations of glutamine, endotoxin and urine lactulose mannito (L/M) ratio were significantly higher in operation treatment AP patients than those in non-operation treatment AP patients. Conclusion In the early course of AP, continued increased intestinal permeability and serum concentration of endotoxin is the indications of operation.

Objective To treat urinary calculi with pneumatic lithotripsy under non-endoscopy.Methodes We applied the pneumatic lithotripsy machine,which made in China,to treat huge or staghom nephrolith and calculi of anterior urethra under non-endoscopy,taken out stone fragmentations after lithotripsy.There was 1 case of huge nephrolith,7cases of staghom nephrolith,3 cases of anterior urethral calculi.Results Every case of nephrolith was successfully performed by using lithotrisy and extracted stones only one case.1 case in total nephrolith cases was found residual stones and sick-side renal functions were improved than before in all casws followed up for 3 moths to 2 years.the cases of anterior urethral calculi urinated fluently and had no urethral stenoses followed up 1～2 years.Conclusion This kind of operation used pneumatic lithotripsy to treat urinary calculi under non-endoscopy is safe,slight injury and curative effect is reliable.