On the basis of ARMT core processor, a biological signal acquisition system with USB2.0 interface is presented in this paper. It is self-developed and Lab VIEW7 is taken as a host communicating software platform for the entire data communication system. The study is mainly focused on the firmware programming for USB chip, the development of USB WDM Driver, and the method for the calling of DLL from Lab VIEW7 to communicate with self-made hardware device.

Objective To analyze retrospectively the factors related with lymph node metastasis in thyroid microcarcinoma.Methods One hundred and eieghty-seven cases of thyroid microcarcinoma were collected from the First Affiliated Hospital of Dalian Medical University from January 2007 to December 2011,who were operated at the first time and confirmed by pathology.According to the occurrence of lymph node metastasis,they were divided into metastasis group and non-metastasis group.The factors related were analyzed retrospectively.Results The total metastasis rate was 26.7％ (50/187),the metastasis rate of central lymph node was 22.9％ (43/187),and the metastasis rate of lateral lymph node was 11.8％ (22/187).Tumor's diameter more than 5 mm,multiple foci and extrathyroidal extension were related to neck lymph node metastasis of thyroid microcarcinoma (P ＜ 0.05).Conclusions Neck lymph node metastasis was seen most common in central lymph nodes.Tumor size,multiple foci and extrathyroidal extension were related to neck lymph node metastasis of thyroid microcarcinoma,operation therapy should be routinely performed.

Objective:To establish criteria of commonly-seen TCM syndromes of depression.Methods:Consultation of specialists,clinical epidemiological survey,scores of syndrome degree rating and statistical analysis of multiple factors were adopted to establish the criteria for main syndrome differentiation of 5 commonly-seen TCM syndromes of depression.Results:Of 1977 cases of depression,1731 cases were stagnation of the liver-Qi,stagnation of the liver-Qi and deficiency of the spleen,stagnation of the liver-Qi and phlegm,deficiency of both the heart and spleen,and Yin-deficiency of both the liver and kidney.And according to statistical analysis,the criteria for main syndrome differentiation of the 5 syndromes were established.Conclusion:Commonly-seen syndromes of depression have common cause of disease and pathogenesis.Stagnation of the liver-Qi is basic syndrome,and in clinical manifestation of various syndromes emotional depression is common main syndrome,but various syndromes still have other main syndrome.

Objective: To investigate the effect of integrin α5 on the expression of NOD-like receptor thermal protein domain associated protein 3 (NLRP3) in periodontal ligament fibroblasts (PDLFs) within an inflammatory microenvironment. Methods: This study was approved by the Ethics Committee of Laboratory animals. After rat PDLFs were treated with LPS (0.5, 5, and 50 µg/mL) for 24 h, the primary medium was discarded and replaced with serum-free culture medium. After 24 h, the supernatant was collected and mixed with DMEM medium containing 10% exosome-free serum at a volume ratio of 1:1 to obtain conditioned medium (CM). The groups were labeled as the 0.5-CM, 5-CM, and 50-CM groups. In addition, PDLFs cultured in DMEM medium containing 10% exosome-free serum were considered the 0-CM group. PDLFs were cultured with the above CM. In the inhibitor group, PDLFs were cultured in 0-CM containing different concentrations of integrin α5 inhibitor ATN-161 (0, 0.025, 0.25, 2.5, 25, and 250 μg/mL). The effect of CM and integrin α5 inhibitor ATN-161 on cell viability was assessed using the CCK-8 assay. According to the CCK-8 results, in further inhibitor intervention experiments, PDLFs were cultured in 0-CM, 5-CM (without/with 25 μg/mL ATN-161), and 0-CM containing 25 μg/mL ATN-161, which were labeled as the 0-CM, 5-CM, ATN-161+5-CM, and ATN-161 groups, respectively. The expression changes of integrin α5 and NLRP3 were detected using Western blot and qRT-PCR techniques. For in vivo experiments, 24 rats were randomly divided into four groups (n=6). The control group contained healthy rats that received no treatment. The rats in the other three groups were injected with 40 µL of 0-CM containing 25 μg/mL ATN-161 or 5-CM (without or with 25 μg/mL ATN-161) on the palatal side of the left maxillary first molar every three days; these groups were classified as the ATN-161, 5-CM, and ATN-161+5-CM groups, respectively. On the 30th day, the left maxillary tissue of rats was used for Micro-CT, HE staining, and immunohistochemical detection. Results : The CCK-8 assay showed that CM, 25 μg/mL ATN-161, and ATN-161 concentrations below 25 μg/mL had no significant effect on cell viability at 12 h and 24 h (P > 0.05). 50-CM and 25 μg/mL ATN-161 significantly inhibited cell viability at 48 h (P < 0.05). For in vitro experiments, compared to the 0-CM group, both the protein and mRNA levels of integrin α5 and NLRP3 were significantly increased in rat PDLFs in the 5-CM group (P < 0.05). Intervention with 25 μg/mL ATN-161 significantly attenuated the enhancement of 5-CM on the expression of integrin α5 and NLRP3 (P < 0.05). For in vivo experiments, compared to the control group, alveolar bone resorption and periodontal inflammatory cell infiltration were significantly increased in the 5-CM and ATN-161+5-CM groups, and the expression of integrin α5 and NLRP3 was significantly increased (P < 0.01). However, compared to the 5-CM group, the ATN-161+5-CM group had less alveolar bone resorption and fewer periodontal inflammatory cells. Further, the expression of integrin α5 and NLRP3 was significantly reduced (P < 0.01). Conclusion In vitro and in vivo experiments showed that integrin α5 mediated NLRP3 expression in PDLFs under an inflammatory microenvironment. ATN-161 inhibited the expression of integrin α5, thus significantly downregulating the expression of NLRP3, which plays a role in inhibiting inflammation.

ObjectiveTo investigate the current status and high-risk factors of chromosomal aberrations in peripheral blood lymphocytes （PBL） of radiation workers in Hainan Province. MethodsA total of 200 radiological workers who underwent occupational health examination in Hainan Provincial Hospital of Traditional Chinese Medicine from January 2021 to December 2021 were selected to collect the occupational health examination data and the rate of PBL chromosomal aberrations. The influencing factors of PBL chromosomal aberrations were analyzed by logistic regression model. The predictive value of logistic regression prediction model on PBL chromosomal aberrations were determined by using the reciver operator characteristic （ROC） curve. ResultsA total of 20 000 cells （100 cells/person） were tested. The chromosomal aberration rate was 0.37% （74/20 000） and the PBL chromosomal aberration rate in the subjects was 6.00% （12/200）. Univariate analysis showed that PBL chromosomal aberrations in radiological workers were related to age， length of service， type of work and education （all P<0.05）， but not to gender （P>0.05）. The logistic regression prediction model was constructed based on the influencing factors， with χ²=9.413， df=9， P=0.852， suggesting a good model fit. The logistic regression prediction model predicted the area under the curve （AUC） for the occurrence of PBL chromosomal aberrations in radiation workers was 0.914 （95%CI： 0.866‒0.949）， with a cut-off value of 3.05， corresponding to a prediction sensitivity and specificity of 100.00% and 75.98%， respectively. ConclusionThe incidence of PBL chromosomal aberrations in radiological workers in Hainan Province was 6.00%， with age， working age and job type as high-risk factors and education level as a protective factor. The prediction model constructed by the above factors can provide a reliable basis for clinical prediction of PBL chromosomal aberrations in radiological workers.

ObjectiveTo analyze the epidemiological characteristics of hepatitis B virus （HBV） infection among blood donors in Hainan Province. MethodsHBV screening was conducted on 6 851 samples from voluntary blood donors in Hainan Province from January 2020 to January 2023. The samples were grouped according to donation frequency， method， age， gender， occupation， and education level， and risk factors related to HBV infection and genotypes were analyzed. ResultsThe HBV infection rate among first-time blood donors （3.59%） was higher than that among repeat donors （0.16%）. Among first-time blood donors， the HBV infection rate in males （2.22%） was higher than in females （1.66%）， and the infection rate increased with age （all P<0.05）. Blood donors who were teachers （1.18%）， militaries （1.01%）， civil servants （1.27%）， and medical staffs （0.85%） had lower HBV infection rate， while workers （6.05%）， farmers （6.15%）， and freelancers （7.61%）， and service industry workers （8.77%） had higher HBV infection rate. The infection rate decreased with higher education levels （P<0.05）. Multivariate logistic regression analysis showed that multiple blood donations （OR=0.623， 95%CI： 0.337‒0.945）， history of intravenous transfusions （OR=1.694，95%CI： 1.517‒1.946）， and family history of HBV infection （OR=1.768， 95%CI： 1.265‒1.934） were independent risk factors for HBV infection （all P<0.05）. Significant differences were observed among different HBV genotypes in terms of HBeAg positivity rate， HBeAb positivity rate， incidence of post hepatitis B cirrhosis， as well as levels of ALT， AST， and HBV-DNA （all P<0.05）. The results of multivariate logistic regression analysis showed that age， HBeAg， HBV-DNA， and clinical type were risk factors for HBV genotype distribution （P<0.05）. ConclusionHainan Province is a low-prevalence area for HBV infection， but the latent risk for HBV infection still exists. Therefore， it is important to thoroughly understand the basic information and HBV infection genotypes of blood donors， so as to select healthy blood donors and reduce the discovery and transmission of HBV-positive cases among blood donors.

OBJECTIVE: To establish an amino acid assay for the determination of β-lactoglobulin in Anti-HPV biological protein dressing. METHODS: Under acidic conditions, β-lactoglobulin is hydrolyzed into free amino acids, separated by cation exchange chromatography, and derivatived after ninhydrin column. The chromatogram at 570 nm is collected. The content of β-lactoglobulin in the sample is indirectly determined by measuring the lysine content obtained by hydrolysis. RESULTS: β-lactoglobulin has a good linear relationship in the concentration range of 77.28~309.12 μg/mL ( CONCLUSIONS The method is simple, specific, accurate and reproducible, which is suitable for the quantitative analysis of β-lactoglobulin in anti-HPV biological protein dressing.
Amino Acids ; Bandages ; Lactoglobulins

Objective To investigate the influence of physiological motions on three-dimensional (3D) CT images and the rules of motion artifacts.Methods A mathematical model of motion artifact was set up based on the principle of spiral scanning, hereby the geometrical distortion of 3D image was analyzed.Experiments were carried out with home-made phantoms on a CT scanner.Results The details of 3D images with trivial distortion were legible under static condition.On the contrary, distinct distortion occured under moving condition, and its degree increased with the pitch and motion period. The 3D images were varied with difference of the motion initial phases.Conclusion The distortion of 3D-CT image depends on the motion characters of scanned objects and scanning parameters such as pitch and slice thickness.

The diagnostic criteria of splenic hemangioma is the delayed filling enhancement pattern on the dynamic contrast CT imaging or magnetic resonance (MR) T1-weighted image,which requires long examination time and thus decreases the MR scanning efficiency. Contrast-enhanced T2 fluid-attenuated inversion recovery (FLAIR) imaging is a new MR imaging technique that can be used to evaluate the intrinsic characteristics of hemangioma. However,few literature has discribed its role in diagnosing splenic hemangioma. In this article we reported one case of splenic hemangioma diagnosed by contrast-enhanced T2 FLAIR imaging,which reduced the MR scanning time and provided valuable experience for the diagnosis of splenic hemangioma.