Through the two-step method FST group and FST6 group both can successfully induce DC into mature functions;the induction in FST6 group is more efficient.

Volatile organic compounds (VOCs) are organic chemicals which can exist in the form of vapor at room temperature.Endogenous VOCs can be produced by different biochemical processes in the human body.They can be transfered to lung through bloodstream and released through breath.Many researches show that exhaled VOCs can be significantly changed when people suffering cancers or inflammation. Detecting exhaled VOCs through instrument with high sensitivity make it possible to detect cancers in early phase in a noninvasive and rapid way,which reduce the suffering of patients.In conclusion,exhaled VOCs detection is a promising method for early diagnosis of cancers.

Objective To find lymphoma cell markers characteristic of gas for breath monitoring application and provide experimental evidence for the early diagnosis of lymphoma patients,assessing solid phase micro extraction-gas chromatography / mass spectrometry( SPME-GC / MS)is applied to the lymphatic the feasibility of detecting tumor cells in the headspace. To investigate the value of VOCs in the diagnosis of hematologic malignancies. Meth-ods The air samples from the headspace of non-Hodgkin lymphoma cell JEOK,the human lymphoidcell lines and culture medium were collected by syringes,and then determined by means of SPME-GC / MS in order to have a bet-ter understanding of the concentration distributions and changes of VOCs in JEOK cells headspace. Using Mann-Whitney U test,we found the characteristic volatile markers of non-Hodgkin′s lymphoma cells. Results SPME-GC /MS for JEOK cell lines,human lymphocytecell line and a blank incubation were detected headspace analysis,found JEOK cell culture headspace can be detected dimethyl,sulfide,toluene,o-xylene,1,3-Di-tert-butylbenzene,aceto-pheno-ne,dodecane,representing human lymphocytes increased;and alcohol,benzaldehyde,hexanal is signifi-cantly reduced. Conclusion Non-Hodgkin′s lymphoma cell lines can cause flasks JEOK headspace change in the composition of VOCs,increased alkanes and aromatic compounds,aldehydes and reducing alcohol content,these substances may be used as acharacteristic of lymphoma cells markers;SPME-GC / MS as a trace substance detection method can be used to JEOK cell lines VOCs bottle headspace detection training.

Objective To investigate the correlated factors of prognosis for acute myeloid leukemia (AML).Methods A retrospective analysis of initial 182 patients with AML was performed to assess the relationship between prognostic factors including sex,age,white cell count at diagnosis,immunophenotype,cytogenetics,allo-HSCT and complete remission (CR) after one-course of induction and overall survival (OS) or event free survival (EFS).Results The median age of 182 patients was 49 (14-80) years old,and the median follow up was 9.7 (0.5-75.5) months.There were 107 patients achieved CR after one-course of induction (total response rate was 65.9 ％,120/182),age≥60 years.CD64 negative,unfavorable cyto-genetics and failing to achieve CR after one-course of induction were associated with lower total CR rate.Univariate analysis results demonstrated that age ≥60 years,unfavorable cytogenetics,CD11b expression and failing to achieve CR after one-course of induction had poor influence on 3-years OS and EFS,while the 3-years OS and EFS for patients with CD19 expression,CD64 expression and allo-HSCT were better than that of the patients with negative CD19 and CD64.Multivariate analysis suggested that age,cytogenetics,CD11b,CD64,allo-HSCT and CR after one-course of induction were independent prognostic factors for OS,while cytogenetics,CD64,allo-HSCT and CR after one-course of induction were important on EFS.Conclusions Prognostic analysis depending on AML corelation factors is benifit to individual therapy and can be applied to improve the survival.

Objective To find out the impact of SHI-1 cells on VOCs in the headspace, and to assess the feasibili-ty of VOCs analysis in leukemia patients or with other tumors in the differential diagnosis by determing VOCs in the headspace of acute mononuclear leukemia cells(SHI-1). Methods The air samples from the headspace of SHI-1, Human macrophages cells and medium control were collected by syringes, and then determined by means of solid phase microextraction-gas chromatography/mass spectrometry ( SPME-GC/MS ) in order to have a better under-standing of the concentration distributions and changes of VOCs in SHI-1 cells' headspace. Results Using Mann-Whitney U test, we found that eight kinds of VOCs were different among them. They were 2,4-dimethyl heptanes, 4-methyl octane, chloroform, benzene, 3,7-dimethyl dodecanese, hexanol, cyclohexanol, cetane. And alkane, benzene were significantly higher than the control group ( P<0.05 ) , alcohol decreased significantly ( P<0.05 ) . Conclusion SHI-1 cells have an impact on the concentration distributions of VOCs in their headspace. The most outstanding VOCs are alkanes, alcohol and benzene compared with the blank control group. They may be potential markers characteristic of leukemia cells.

Objective:To investigate the serum level of IL-34 in patients with acute phase Henoch-Schoenlein purpura ( HSP) and its potential significance .Methods:38 HSP patients were recruited ,together with 21 healthy volunteers as control .The serum level of IL-34,IL-6 and TNF-αwere determined using enzyme-linked immunosorbent assay (ELISA) or chemiluminescence.The correlation analysis was performed to explore the correlation of IL-34 with clinical parameters and the serum level of IL-6,TNF-α.Results:The se-rum levels of IL-34,IL-6 and TNF-αin HSP patients were significantly higher as compared to the control group (P<0.001).The serum level of IL-34 was positively correlated with that of high sensitive C-reactive protein ( hs-CRP) and IL-6 ( r=0.453 ,P=0.004 and r=0.469,P=0.003,respectively).Conclusion:IL-34 might be involved in the pathogenesis of HSP ,and the potential mechanisms of ac-tion is associated with the aberrant expression of pro-inflammatory factors in HSP ,which promote the development of inflammation in the vascular system.

Objective: To investigate the effect of Atractyloside on sevoflurane postconditioning in focal cerebral ischemia.Methods: Fifty male SD rats were randomly assigned to five groups(n=10 each): control group(con),1.0 MAC group(sevo),1.0 MAC+ Atractyloside group(sevo+Atr),1.0 MAC+ vehicle group(sevo+vehicle)and Atractyloside group(Atr).All animals were subjected to the right middle cerebral artery occlusion(MCAO) for 120 min followed by reperfusion for 72 h.The animals in sevo groups were given 1.0 MAC sevoflurane inhalation from 20 min before to 10 min after reperfusion.The animals in Atr groups were given Atractyloside by ICV injection before sevoflurane postcondioning.The neurological deficit scores(NDS) were recorded at 24 h,48 h,and 72 h after reperfusion.Infarct volume percentage was determined after the last NDS assessment.Results: The infarct volume percentage ratio of sevo group(0.32?0.05) was significantly lower than that of con group(0.55?0.07)(P0.05).The infarct volume percentage ratio of sevo+Atr group and Atr group were significantly higher than that of Sevo group(P0.05).NDS of 24 h,48 h and 72 h after reperfusion in sevo group and sevo+vehicle were significantly higher than that in Con group.NDS of 24 h,48 h and 72 h after reperfusion in sevo+Atr group and Atr were significantly lower than that in Sevo group.Conclusion: Atractyloside abolished the protective effects of sevoflurane postconditioning on focal cerebral ischemia-reperfusion injury.

AIM:To elucidate whether ZFP580 is involved in the cardioprotective effects of hypoxic precondi-tioning (HPC) against hypoxia-reoxygenation (H/R) injury in H9c2 myocardial cells.METHODS: Rat heart-derived H9c2 cells were cultured in DMEM .H/R was induced by incubation under ischemic hypoxia for 3 h and reoxygenation for 2 h.HPC was induced by exposing the H 9c2 cells to 10 min of hypoxia and 20 min of reoxygenation for 3 cycles before H/R treatment.MTT staining and LDH leakage detection were used to evaluate the effects of HPC .Western blotting was used to detect the protein levels of ZFP580, phosphorylated ERK1/2 and cleaved caspased-3.The effects of ZFP580 overexpre-ssion or knockdown on H/R induced apoptosis were determined .RESULTS:The results of MTT staining and LDH leakage detection showed evidence of HPC cytoprotection against H /R-induced cell death in H9c2 cells.ZFP580 protein level and ERK1/2 phosphorylation were significantly increased in the HPC group compared with control group and H /R group. PD98059, an inhibitor of ERK1/2 phosphorylation , significantly suppressed the HPC-induced up-regulation of ZFP580 pro-tein expression.ZFP580 overexpression significantly inhibited apoptosis and caspase-3 activation in H9c2 cells.CON-CLUSION:HPC exhibits cytoprotection against H/R and leads to high level of ZFP 580 protein in H9c2 cells.ZFP580 is regulated by ERK1/2 activation and mediates the anti-apoptotic effect of the ERK1/2 signaling pathway in HPC cytoprotec-tion.

Objective To study the clinical values of polyvinyl alcohol chemoembolization and bronchial arterial infusion(BAI) in interventional therapy of central bronchogenic carcinoma.Methods 45 pathologically proved cases of central bronchogenic carcinoma in mid or late period were randomly divided into two groups(control group in 24 cases and experimental group in 21 cases).After detecting the nourishing arteries of bronchogenic carcinoma with angiography,the treating processes were performed.Control group : anticancer drugs were poured into bronchial artery.Experimental group : the feeding arteries of tumors were co-axially superselective catheterized with microcatheters,the anti-neoplasm agents were poured into everyone of feeding arteries according to the blood supply,then the feeding arteries were embolized by polyvinyl alcohol.Results Control group : the obvious effective rate(CR+PR) was 50%.Experimental group:the obvious effective rate was 90.4%.Two cases in control group obtained the chances of radical operation and chemotherapy were performed after operation,the other 22 cases were undergone synthetic treatment.Seven cases in experimental group obtained the chances of radical operation and chemotherapy were performed after operation,14 cases were undergone synthetic treatment.Three cases in control group died and none of in experimental group died in the follow-up of 2~24 months.Conclusion The short-term effect of polyvinyl alcohol embolization is better than those of BAI for interventional therapy of central bronchogenic cancer,but the long-term effect needs a large number of cases to access the real clinical value.

Objective:Ischemic cerebral injury is a common complication during perioperative period.We found that ischemic postconditioning and various non-ischemic postconditioning(e.g.inhalation anesthetics) could significantly attenuate ischemic cerebral injury.The present study was to investigate the effect of Cyclosporin A on sevoflurane postconditioning in focal cerebral ischemia.Methods:Fifty male SD rats were randomly assigned to five groups (n=10 each): control group(con),1.0MAC group(sevo),1.0MAC+ Cyclosporin A group(sevo+CsA),1.0MAC+ vehicle group(sevo+vehicle)and Cyclosporin A group(CsA).All animals were subjected to right middle cerebral artery occlusion(MCAO) for 120min followed by reperfusion for 72h.The animals in sevo groups were given 1.0MAC sevoflurane inhalation from 20min before to 10min after reperfusion.The animals in CsA groups were given CsA by ICV injection before sevoflurane postconditioning.The neurological deficit scores(NDS) were recorded at 24h,48h,and 72h after reperfusion.Infarct volume percentage was determined after the last NDS assessment.Results:The infarct volume percentage ratio of Sevo, Sevo+CsA,CsA and Sevo+vehicle groups were 0.31?0.04,0.25?0.04,0.30?0.03 and 0.33?0.05,respectively(P