In recent years,experts of international nephrology,emergency medicine and other disciplines tend to substitute acute kidney injury for acute renal failure.There is a new point of view about the definition,diagnostic criteria,staging,management and prognosis of acute kidney injury.With diagnostic criteria constantly updated,it comes up to an expectation of united criteria as well as reaching a consensus.Continuously looking for more suitable for both time window of clinical diagnosis and clinical observation indicators,and clinically making out much more scientific and reasonable standard of diagnosis and staging,which contribute to early detection and early intervention as a result of eventually mortality rate reduction.This paper gives an interpretation for the procedure of evolution about diagnostic criteria on acute kidney injury in children according to the related literatures updated in recent years.

Acute kidney injury(AKI) is a common critical syndrome involving multidisciplinary among pediatric clinical disorders.Early identification and early intervention in severe cases with kidney damage,which can reduce mortality and improve prognosis.Renal replacement therapy(RRT) is an important means in the treatment of AKI,but there is still lacking of ideal and general modality and doses of RRT at present.Understanding the risks and benefits of each modality of RRT in the treatment of AKI is helpful to evaluate RRT's influence on the prognosis of children in AKI.Therefore the selection of modality of RRT should clinically be based on hospital conditions and specific patient characteristics,in order to assure that the choice of modality of RRT is the most suitable for the individual.

Objective To develop the scale on work values for college students. Methods 63 items were determined through reviewing items in related literatures and scales, experts consulting and panel discussion.528 students from 3 universities in Shanghai were enrolled in the study. The data were analyzed through exploratory factor analysis,discrimination analysis,reliability analysis and other statistical analysis methods. Results College students' work values comprises six dimensions: self-value dimension ( including two sub-dimensions: self-esteem dimension and self-realization dimension), social-value dimension, dimension of the working environment, wages and welfare dimension , job stability dimension, and the dimension of social status. 43 items were reserved after exploratory factor analyses. The coefficient of reliability of the 43 items was 0.943, and the item loading of the 43 items was 0. 321 ～ 0. 792. Conclusion The discernment, internal consistency and validity of the scale accord with psychometric demands.

Isolated pancreatic islet cells from neonatal rats were cultured in vitro. Insulin release was significantly inhibited by interleukin-1?. Addition of testosterone to the IL-, 1?-impaired pancreatic islets could increase insulin release, suggesting a protective effect of testosterone on pancreatic islet cells. Testosterone also significantly increased the insulin content in islet cells, and this effect could be blocked by actinomycin-D, indicating that testosterone promoted the synthesis of DNA and insulin may be one of its major protective mechanism.

Objective: To further explore the mechanism by which retinoic acid (RA) promotes the antibody production of cord blood lymphocytes (CBL). [WT5FZ]Methods: The levels of serum vitamin A in cord blood were measured. The expression of retinoic acid receptor ? (RAR ?) gene was determined by RT PCR (reverse transcription polymerase chain reaction). The IgM concentrations in the supernatants of cell culture were measured by ELISA. [WT5FZ]Results: A positive correlation was found between serum vitamin A level and RAR ? expression level in uncultured CBL:r=0.50 (P

To develop a new temperature control moxibustion device so as to improve the clinical therapeutic effect of moxibustion. According to the thermal effect of moxibustion, with the designs such as the modern electronic equipment (temperature control system) adopted and in combination of smoke filtration device and oxygen mask device, a new temperature control moxibustion device was developed. The new temperature control moxibustion device may achieve the automatic regulation of temperature and distance and avoid the pollution and irritation of smoke and flavor, etc. As a result, the traditional moxibustion therapy can better play its efficacy and display its safety and convenience in practice.
Equipment Design ; Humans ; Moxibustion ; instrumentation ; methods ; Temperature

Objective To investigate the changes of serum transforming growth factor-β1 (TGF-β1),interleukin-10(IL-10) and interferon-γ(INF-γ) in elderly patients with hepatic fibrosis and their correlation with hepatic fibrosis.Methods One hundred and thirty-three patients diagnosed by pathological hepatic tissue were enrolled as case group who were in the first affiliated hospital of Xinxiang Medical University from 2007 to 2013.The enzyme-linked immunosorbent assay (ELISA) was applied to detect of serum levels of TGF-β1,IL-10and IFN-γ.Meanwhile 21 healthy people were recruited as control group.Results Comparison with the control group,the levels of TGF-β1 and IL-10 were significantly higher(TGF-β1:(217.4 ± 7.5) ng/L vs.(57.2±4.8) ng/L,t =15.61,P＜0.01;IL-10:(29.6 ±3.2) ng/L vs.(15.1 ±7.5) ng/L,t =11.44,P＜0.01),while IFN-γlevel was lower ((51.9 ± 3.5) ng/L vs.(75.8 ± 5.9) ng/L,P =0.03) in case group.The serum levels of TGF-β1,IL-10,IFN-γand stages of hepatic fibrosis was correlated and the correlation coefficients were 0.786,0.644 and-0.822 respectively (P ＜ 0.05).Conclusion Serum levels of TGF-β1,IL-10 and IFN-γ in patients with hepatic fibrosis are proved to be with ability of predicting the development of hepatic fibrosis and the post treatment.

OBJECTIVE: To improve the quality, efficiency and management of hospital pharmacy dispensing work. METHODS: Following the principle of logistics, the drug dispensing path was planned as a whole to reduce the ineffective drug handling, and the dispensing process was designed and machines were reasonable utilized. RESULT & CONCLUSION: Improving pharmacy dispensing work in line with the principle of logistics is conducive to the improving of both quality and efficiency of dispensing work, strengthening of management and lowering of labor intensity.

Ursolic acid, a pentacyclic triterpene compound, naturally occurs in a large variety of plants, has at-tracted considerable interest owing to its significant biological activities. In recent years, more and more scientists have been working on the structural modification at the C-3 position , C_(12)-C_(13) double bond or C-28 position of ur-solic acid in order to improve their biological activities. Herein a brief introduction of the recent progresses on the chemical structural modification and the structure-activity relationship of ursolic acid and its derivatives are re-viewed.

ObjectiveTo investigate the relationship between metastasis-associated gene 1 ( MTA1 )expression and invasive and metastatic ability of cervical cancer cell. MethodsThree kinds of plasmids pcDNA3( control group), pcDNA3-MTA1 ( MTA1 group) and pSilencer3. 1-MTA1-siRNA ( MTA1-siRNAgroup) were transfected into human cervical cancer cell line CaSki cells. Reverse transcription (RT)-PCR and western blot were used to detected MTA1 mRNA and protein expressions. The effects of MTA1 expression on CaSki cell growth and proliferation, cell migration, adhesion and invasion, and cell cycles were tested by methyl thiazolyl tetrazolium (MTT), clone formation experiment, wound-healing assay, transwell assay, adhesion assay and flow cytometry, respectively. In animal experiment, three groups of cells were inoculated to BALB/c nude mouse subcutaneously to observe tumor formation ability. ResultsCompared with control group, MTA1 mRNA and protein were significantly overexpressed in MTA1 group, while MTA1-siRNA group showed lower MTA1 expression. Compared with control group, MTA1 group showed significantly accelerated cell growth; while MTA1-siRNA group showed decreased cell growth since the second day (P＜0. 05). Clone formation number in control, MTA1 and MTA1-siRNA group were 133 ±6, 169 ± 10 and 57 ±5,respectively. MTA1 group showed accelerated cell formation, while MTA1-siRNA group showed the reverse effect compared with that in control group(P ＜ 0. 05 ). At 24, 48 and 72 hours after wounding, the healing ability of MTA1-siRNA group significantly lagged behind that in the control group, while MTA1 group showed accelerated cell healing ability. The adhesion rate of control, MTA1 and MTA1-siRNA group were (69. 3 ± 3. 6) ％, ( 80. 4 ± 5. 6 ) ％ and ( 39. 2 ± 7.4 ) ％ separately at 90 minutes after cell seeding. In contrast with control group, MTA1 group promoted the adhesion of CaSki cell to matrigel matrix, while MTA1-siRNA group inhibited the adhesion process (P ＜0. 05 ). In the migration assay, the number of cells migrated to the bottom side of the membrane in control,MTA1 and MTA1-siRNA group were 153 ± 17,247 ± 38 and 82 ± 10, respectively. The number of cells in the invasion assay were 231 ± 19,354 ± 36 and 76 ± 7, respectively. Compared with the control group, MTA1 group significantly increased the migration and invasion ability, while MTA 1-siRNA group showed lower cell migration and invasion ability (P ＜ 0. 05 ). In cell cycle experiment, no significant differences of cell proportions including G1, S and G2 stage were found among three groups (P ＞ 0.05).In animal experiment, compared with control group,MTA1 group showed accelersted tumor formation and growth,whilethe MTA1-siRNA group showed the reverse effect ( P ＜ 0. 05 ). ConclusionsMTA1 may play its roles to promote cervical cancer cell invasion, migration, adhesion, as well as cell growth and colony formation, while RNA interference against MTA1 may decrease the malignant phenotypes. This study shows that it will be an effective beginning to explore metastasis mechanisms and cancer gene therapy strategy targeting MTA1 in cervical cancer.