1.Clinical Study of Combined Therapeutic Effect of Lipo PGE_1 and Millimeter Wave on Diabetic Peripheral Neuropathy
Jie SHAN ; Xiangping LUAN ; Haili LIU
Journal of Medical Research 2006;0(07):-
Objective To study the clinical effect of combined treatment of Lipo PGE1 and millimeter wave on diabetic peripheral neuropathy.Methods 98 patients with diabetic peripheral neuropathy(DPN)were randomly divided into the first treatment group with Lipo-PGE1,and the second treatment group combined with Lipo PGE1 and millimeter wave compared with the routin therapy group as control in order to observe the subjective symptom,tendon reflex and nerve conduction velocity,respectively.Results The total effective rates of the second treatment group was 91%,which was significantly higher than the control group(P
2.PUMA gene in cancer treatment
Qingchun LUAN ; Haijuan WANG ; Haili QIAN ; Yan CHEN ; Chen LIN
Journal of International Oncology 2011;38(11):803-805
PUMA (p53 up-regulated modulator of apoptosis) is a recently discovered Bcl-2 family member which could be rapidly induced by p53 and has strong pro-apoptotic effects.PUMA has attracted much attention in the research of life science.PUMA expression results in potent growth suppression of some cancer cells through induction of apoptosis.PUMA can also significantly sensitize some cancer cells to chemotherapeutic agents and irradiation through induction of apoptosis.PUMA is potentially useful in gene therapy of tumor.But recently,researchers have also found that PUMA participates in the process of carcinogenesis and possessed important biological functions.
3.Bronchoscopic characteristics of mycoplasma pneumoniae in children and the level of inflammatory factors in bronchoalveolar lavage fluid
Xiaoyun SHI ; Haili LUAN ; Han ZHANG ; Yunxiao SHANG
International Journal of Pediatrics 2017;44(12):867-871
Objective To investigate the specpfic electric fiberobronchoscopic manifestations of refractory Mycoplasma pneumoniae pneumonia(RMPP) and explore the level of IL-17,IL-18,PTX3 in bronchoalveolar lavage fluid(BALF) and their clinical sigfinance.Methods To select patients who were diagnosed as MPP and examined by fiberobronchoscopy in acute stage.Dividing them into groups:(1) RMPP group (60cases):dividing RMPP patients into three groups according to if they were treated by systemic corticosteroids or immunoglobulin before the examination of fiberbronchoscope,① RMPP-A group:both are not used.②RMPP-B group:use systemic corticosteroids.③ RMPP-C:both are used.(2)general MPP group(35 cases).15 children with foreign body in bronchus were enrolled as control group.Firstly,to analysis the electric fiberobronchoscopic manifestations of all the cases.Secondly,the cases who had BALF samples in all group were selected,the levels of IL-17,IL-18 and PTX3 are detected by ELISA.Results Under electric fiberobronchoscopy,that the proportions of RMPP group with mucosal erosion,necrotic mucous membrane peeling,sputum bolt blockage in bronchial lumen or moulding are higher than general MPP group.The levels of IL-17,IL-18 and PTX3 in BALF of all MPP cases are higher than control group (P < 0.05),but only the difference of IL-18 between RMPP group and general MPP group has statistical significance (P < 0.05).The levels of IL-17,IL-18 and PTX3 in BALF of RMPP-B group are all higher than RMPP-A group (P < 0.05).Conclusion Mucosal erosion,necrotic mucosa peeling and sputum bolt,moulding are the characteristic manifestations of RMPP,and can help identify RMPP.IL-17,IL-l8 and PTX3 all participated in the pathogenesis of RMPP.Only the level of IL-18 in BALF can be the predictive marker of RMPP.Systemic corticosteroids may inhibit the levels of IL-17,IL-18 and PTX3 of RMPP patients.
4.The clinical significance of T-cell subset,cytokine levels in bronchoalveolar lavage fluid of children with mycoplasma pneumoniae pneumonia
Haili LUAN ; Han ZHANG ; Yunxiao SHANG
Chinese Pediatric Emergency Medicine 2017;24(11):850-854
Objective To explore the levels and clinical significances of T-cell subset,interleukin (IL)-6,interferon(IFN)-γ,and monocyte chemotactic protein(MCP)-1 in bronchoalveolar lavage fluid (BALF) of children with mycoplasma pneumoniae pneumonia(MPP).Methods Thirty-six children with pneumonia were admitted in our hospital from October 2014 to March 2016.They were divided into refractory mycoplasma pneumoniae pneumonia (RMPP) group (n=18) and MPP group(n=18).Sixteen cases with bronchial foreign body were selected as the control group.The levels of IL-6,MCP-1,IFN-γ and T-cell subset in BALF were detected by ELISA and alkaline phosphatase-anti-alkaline phosphatase(APAAP) bridging enzyme linked immunosorbent assay.Results (1)The levels of CD3 +and CD8 +T cells in BALF of MPP group and RMPP group were higher than those in the control group(P<0.05),but there were no significant differences between the two groups (P>0.05).(2) The level of IL-6 in acute phase of RMPP group was higher than that in the control group(P<0.05).The levels of IL-6 in acute phase of RMPP group and MPP group were both higher than that in recovery period(P<0.05,respectively).(3)The levels of IFN-γ in the acute phase of RMPP group and MPP group were higher than that in the control group,and the differences were statistically significant(P<0.05).The IFN-γ levels in acute and recovery phases of RMPP group were both significantly higher than those in MPP group respectively(P< 0.05).(4)The level of MCP-1 in BALF in control group was lower than those in the acute phase of RMPP group and MPP group(P<0.05).The level of MCP-1 in RMPP acute group was significantly higher than that in MPP acute group,and higher in acute phase than those in recovery phase both in two groups(P<0.05).Conclusion There are cell immune regulation and function disorders when children are infected mycoplasma pneumoniae. IL-6, IFN-γ and MCP-1 may participate in the pathogenesis of MPP,and more importantly,IFN-γ and MCP-1 may be the important indicator to forecast the severity of MPP.
5.Expression of severe fever with thrombocytopenia syndrome virus Gn-D Ⅲ-Ⅲ and development of indirect ELISA for antibody detection
Mengyao ZHANG ; Tianlai LIANG ; Feihu YAN ; Tao CHEN ; Cuicui JIAO ; Hongli JIN ; Jiaoyan LUAN ; Xiao WU ; Pei HUANG ; Haili ZHANG ; Qin NING ; Hualei WANG ; Yuanyuan LI
Chinese Journal of Veterinary Science 2024;44(8):1704-1712
The PCR-amplified severe fever with thrombocytopenia syndrome virus(SFTSV)Gn-DⅢ-Ⅲ gene was inserted into the pET-30a(+)prokaryotic expression vector to generate the re-combinant plasmid pET-SFTSV-Gn-D Ⅲ-Ⅲ.The plasmid was transformed into E.coli BL21(DE3)for Gn-DⅢ-m protein expression and the expression conditions were optimized.The Gn-DⅢ-Ⅲ protein purified with Ni-NTA column affinity chromatography was applied as the captured antigen to establish an indirect ELISA method for the detection of SFTSV antibody.The results demonstrated that the recombinant plasmid pET-SFTSV-Gn-D Ⅲ-Ⅲ was successfully constructed as identified by PCR and sequencing.The recombinant protein SFTSV Gn-D m-Ⅲ was soluble ex-pression in E.coli under the optimal induction conditions of 0.4 mmol/L IPTG at 25 ℃ for 4 h,and the protein purity was 91.77%after purification by Ni-NTA column.The optimal reaction con-ditions for the indirect ELISA of SFTSV antibody were as follows:coating antigen concentration(5 μg/mL),primary antibody(incubation at 37 ℃ for 1.5 h),and secondary antibody(diluted 1:10 000 and incubated at 37 ℃ for 1 h).The established method had no cross-reactivity with Rift Valley fever virus(RVFV),Ebola virus(EBOV),and tick-borne encephalitis virus(TBEV)posi-tive sera.The method had a high sensitivity,with P/N>2.1 for SFTSV-positive sera diluted to 81920.Coefficients of variation for intra-and inter-batch reactions were less than 10%.Detection of four SFTSV-infected human clinical serum samples showed the serum samples from patients in re-mission were tested as positive(P/N>2.1),while serum samples from patients with multiple or-gan failure were detected as negative(P/N<2.1).The results indicated that the SFTSV Gn-D Ⅲ-Ⅲ protein was successfully expressed and purified,and it was used as the coating protein to estab-lish an indirect ELISA assay for SFTSV antibody,which possesses good specificity,sensitivity and reproducibility.This method might be applied to detect human SFTSV clinical serum samples.