Objective To evaluate the effect of social interaction model on the internal clinical practice and to explore the teaching model which can improve the quality of medical students in clinical medical practice.Methods Totally 261 students of practiced in department of internal medicine from July 2012 to June 2013 were enrolled and were randomly divided two different teaching teams:traditional teaching group (n=130) and social interaction group (n=131).Results of theory examination,skills assessment,medical record writing test and medical humanities appraisal in two groups were compared.Questionnaire was employed to investigate the effect of social interaction model on the internal clinical practice.SPSS 17.0 software was used to do statistical analysis and measurement data was expressed x ± s.Two-sample mean was analyzed by t test andx2 test was used to compare the ratio.P＜0.05 stands for the statistically significant difference.Results There was no statistically significant difference in theory resuhs between traditional teaching group and social interaction model group(P=0.108).Results of medical record writing test and medical humanities appraisal in social interaction group were obviously higher than those of traditional teaching group(P=0.013,0.009).Number of class A medical record and qualified students in clinical skill examination was obviously increased in social interaction group than in traditional teaching group(P＜0.05).Most students held a positive attitude to the social interactive teaching model.Conclusions Social interaction teaching helps to improve the quality of clinical internal medical practice,especially the improvement of clinical practice skills and the cultivation of medical humanities knowledge of medical students.

OBJECTIVE:To establish a HPLC procedure to determine diclofenac sodium in human plasma.METHODS:The plasma sample was deproteinized by adding acetonitrile,A Hypersil-BDS(250?4.6mm,5?m)column with a mobile phase consisting of methanol-water-10%phosphate acid(60∶40∶1)was used,the detecting wavelength was276nm,column tem?perature was35℃.RESULTS:The linear range was20.50～2050.0ng/ml(r=0.9998,n=5),the detective limit was10ng/ml.The recovery was99.47%～105.4%with the within-day RSD from3.77%to5.81%(n=5),and the between-day RSD from4.93to8.96%(n=5)at three different concentrations.CONCLUSION:The method is simple,rapid and suitable for the pharmacokinetic study of diclofenac sodium.

Neuropathies are complications of cardiac surgery. The reported frequency of nerve injuries varies from 1. 5% to 2. 4% for the brachial plexus and 10% to 60% for the phrenic nerve. But the recurrent laryngea! nerve,lower limb (saphenous) nerves, and the sympathetic chain are less frequently injured. The reasons of neuropathy are mainly positioning and topical hypothermia for myocardial protection. The overall prognosis of neuropathies after cardiac surgery is good, and they need no special treatment. However, prolonged recovery of phrenic neuropathy did occurr.

Objective: To study the effect of zinc supplement on bone-contacting implant surface ratio (BCSR).Methods: 40 rabbits with titanium implants placed into the proximal tibial heads were divided randomly into zinc supplement group(n=20) and control group(n=20). 1% zinc sulfate in 0.9% saline was administered intramuscularly at the dose of 4 mg/(kg?d) for each tested animal, and the same amount of 0.9% saline was used for the controls. The animals were sacrificed 1, 2, 4, 8 and 12 weeks after treatment respectively.BCSR,bone zinc content and serum Cu/Zn values were measured with morphometry, atomic absorption photometry and atomic nebulizntion absorption method respectively.Results: Zinc supplement could significantly increase the BCSR value (P0.05). Conclusion: Moderate zinc supplement may increase the bone-contacting implant surface ratio, but not alter the zinc metabolism.

Porous inorganic materials are solid-state compounds with regular porous structure.crystal or amorphous,which are closely related to the biochemical processes and wide applied in industry and daily life.Porous inorganic materials are a new kind Of functional materials due to their intriguing molecular topologies and wide distribution range of internal pore size.They have attracted tremendous attention due to comprehensive potential applications in ion exchange.selective adsorption.and catalysiS.This paper reviews researches and prospects of the applications of porous inorganic materials in the field of biology and medicine recently.Applications of porous inorganic materials in the field of biology and medicine mostly include the enzyme immobilizing,enzyme simulation. bioiogical sensors,controlled release drug carrier,antibacterial and auxiliary of anticancer drugs,etc.

Purpose:To study the expression of survivin a nd its relationship with the expression of bcl-2,p53,PCNA in the dysplasia of gastric mucous epithelium and gastric carcinoma.Methods:The expression of survivin,bcl-2,p53,PCNA was detected in 60 cases of gastric carcinoma, 30 cases of dysplasia of gastric mucous epithelim and 8 cases of normal gastric mucous tissue by using immunohistochemical SP method. Results:The positive rates of survivin were 10%,13%,80% and 82%, respectively, in mild, moderate and svere dysplasia and gastric carcinoma. The positive rates of survivin in mild and moderate dy splasia were significantly lower than in severe dysplasia and carcinoma (P0 05). There was a relationship between survivin gene expression with the invasive depth of gastric carcinoma. lymph node metastases and survival stage (P

OBJECTIVE:To investigate the efficacy of compound azintamide enteric-coated tablet plus mosapride citrate dispersible tablet for abdominal distention in patients with functional dyspepsia(FD).METHODS:A total of 80 patients with the chief complaint of abdominal distention were randomized to treatment group(Group A,n=40) to be treated with mosapride citrate dispersed tablets plus compound azintamide enteric-coated tablet,or control group in which the patients were treated bu mosapride citrate dispersed tablets(Group B,n=20) or compound azintamide enteric-coated tablets(Group C,n=20).Comp-ound azintamide enteric-coated tablets were administered orally tid(2 tablets) immediately after meal while the mosapride citrate dispersed tablets(5mg) were administered orally tid 30min before meal for 2 weeks.The main outcome measures were changes of symptom scores of abdominal distension,the total effective rates in improvement of abdominal distension,and drug side effects before and after treatment.RESULTS:After 1-week and 2-week treatment,the average index scores of abdominal distention in the treatment group(Group A) were significantly lower than in the control groups(Group B and Group C) (P

Objective To analyze the expression of HLJ1 in the tissues of hepatocellular carcinoma(HCC) and the correlation between its expression and clinical pathological characteristic.Methods HLJ1 mRNA expression level was determined in 32 HCC tissues and their associated noncancerous liver tissues by reverse transcription polymerase chain reaction(RT-PCR).The correlation of HLJ1 expression level with clinicopathologic variables was also analyzed.Results HLJ1 mRNA expression level was significantly lower in HCC than in associated noncancerous liver tissue [(1.18?0.82) vs.(1.92?1.15),P

Objective:To investigate the specific immune act ivated effects in the mouse immunized by MHC I-restriced tumor antigen peptide pulsed dendritic cells(DC) with IL-2 gene modified.Methods:DC were transfected with IL-2 gene via adenovirus vector(DC-IL-2) the mRNA of mIL-2 was examined by RT-PCR.Syngeneic mice were immunized by DC-IL-2 pul sed with MHC-I-restricted Mutl tumor antigen peptiede of 3LL Lewis lung carcin oma (DC-IL-2-Mutl),the effects of lymphocyte subsets in the draining lymphoid node from the tumor antigen pulsed DCs with gene modified immunized mice by flo w-cytometric analysis(FACS) analysis.Results:The mRNA of mIL-2 was expressed in the DCs.The ratio of CD8+T cells was hosted in the dra ining lymphoid node from Mutl pulsed DCs(DC-Mutl) immunized mice.The ratio of C D8+T cells and NK cells were all hosted obviously in the draining lymphoid nod e from DC-IL-2-Mutl immunized mice .Conclusion:Immunized mice with DC-IL-2-Mutl can induce the mouse specific antitumor immunized affect in vivo and can activate a variety of immunized effect in the mouse.

Objective: To compare postoperative recovery situation between laparoscopic radical resection and conventional open surgery for patients with colorectal cancer and determine the optimal surgery scheme. Methods: 96 patients with colorectal cancer were divided into observation group (48 cases)and control group (48 cases) according to the random number table method. The patients of observation group were treated by using laparoscopic radical resection while the patients of control group were treated by conventional open surgery. A series of clinical manifestation, such as perioperative situation, abdominal pain, recovery situation, complication, pre-and post operative nutrition and quality of life, of these patients were compared between the two groups. Results: The operation time, average bleeding volume, postoperative exhaust time and hospitalization time of the observation group were significantly less than those of the control group (t=5.583, t=48.934, t=7.347, t=4.862; P<0.05). The incidence of abdominal complication of observation group (8.3%) was significantly lower than that of the control group (27.1%) (x2=5.79, P<0.05). The incidence of delayed healing of incision was 6.3% in observation group, which was significantly lower than that (20.8%) of control group (x2=4.36, P<0.05). For observation group or control group, the serum ALB, PA and LC of post-operation, respectively, was higher than those of pre-operation in 1 week after operation. And serum ALB, PA and LC of observation group, respectively, was significantly higher than that of control group (t=4.628, t=3.642, t=3.412; P<0.05). Compared with before operation, physical function, role function, emotional function, cognitive function and social functionthe quality of life of the two group were obviously improved, on the other hand, all of these aspects of the observation group were significantly higher than those of the control group (t=-12.845, t=-7.634, t=0.5001, t=-4.096, t=-2.647, P<0.05). Conclusion: Compared with the traditional open surgery for patients with colorectal cancer, the postoperative recovery of laparoscopic radical resection is better, more obvious and more effective for them.