Objective To evaluate the effect of pre-exercise on immune response to influenza vaccine(InfV) in healthy adults by Meta-analysis, so as to provide evidence-based basis for the study of "exercise adjuvant" of InfV immune response.Methods PubMed, Cochrane Library, EMbase, Web of Science database, CNKI, Wanfang Data Knowledge Service Platform(Wanfang), and SinoMed were searched to screen the research literature of randomized controlled trials(RCTs) about the effect of pre-exercise on immune response to InfV in adults. Cochrane bias risk assessment tool was used to evaluate literature quality, and Stata 15.0 software was used for Meta-analysis.Results A total of 21 RCT studies on the effect of pre-exercise intervention on immune response to InfV were included, with 702 subjects in the experimental group and 698 subjects in the control group. There was a low probability of risk bias in the included studies. Meta-analysis showed that, compared with the control group, pre-exercise significantly increased the antibody titer and serum protection rate [OR = 1. 38(95% CI: 1. 20-1. 56), P < 0. 05] of influenza virus A H1N1 [MD = 0. 47(95% CI: 0. 36-0. 58), P < 0. 05], influenza virus A H3N2 [MD =0. 34(95% CI:0. 21-0. 47), P < 0. 05], and influenza virus B [MD = 0. 34(95% CI: 0. 22-0. 46), P < 0. 05] in adults after InfV vaccination. The results of subgroup analysis showed that chronic pre-exercise significantly increased the influenza virus antibody titer and serum protection rate [OR = 1. 42(95% CI: 1. 22-1. 62), P < 0. 05] in adults after InfV vaccination,including influenza virus A H1N1[MD = 0. 72(95% CI: 0. 54-0. 90), P < 0. 05], influenza virus A H3N2 [MD = 0. 52(95%CI: 0. 15-1. 09), P < 0. 05], and influenza virus B [MD = 0. 81(95% CI: 0. 53-1. 09), P < 0. 05]. The effect of acute preexercise on antibody titers of type A H3N2 [MD = 0. 14(95% CI: 0-0. 29), P > 0. 05] and influenza virus B [MD = 0. 22(95% CI: 0. 09-0. 36), P > 0. 05] after InfV vaccination still needs further verification. Age may be a factor limiting the titer of influenza virus antibodies in subjects receiving InfV vaccination after pre-exercise.Conclusion Pre-exercise intervention can effectively improve the immune response after InfV vaccination in adults. Follow-up high-quality studies are needed to identify exercise regimens that improve immune response to InfV.

Objective: To explore the suitable load of endurance training for primary school students with different BMI levels living in 3 200 m plateau, and to provide guidance for exercise and weight control for children residing in high altitudes. Methods: The heart rate deflection point (HRDP) and HRDPspeed of 7-12 year-old students residing at high altitude were evaluated by using the site Conconi test. The serum lipid metabolism level was detected by ELISA. The correlation between HRDP and lipid metabolism was analyzed by Pearson correlation analysis. Results: The level of leptin, adiponectin, total cholesterol(TC) and triglycerides(TG) in obesity group was highest, follow by the overweight group, normal weight and under-weight group(F=3.75-24.12, P<0.05). In the same age group, hrdpspeed decreased with the increase of BMI. For children with the same BMI classification, HRDPspeed decreased with age. HRDPspeed showed an increasing trend by age and BMI. In obese, normal and emaciated students, there was a significant negative correlation between lipid metabolism and HRDP, but there was no significant correlation between lipid metabolism and HRDP in overweight students of different ages (P>0.05). Conclusion It is suggested that HRDP and lipid metabolism may be signcficantly correlated with BMI in students aged 7-12 years at different BMI levels in plateav and the above indicators can be used as an effective means to evaluate aerobic exercise endurance capacity of healthy adults at plateau.

Objective: To discuss the correlation between aerobic exercise ability, pulmonary function and heart rate recovery ability of 7-12 year-old students under different altitudes, so as to provide evidence on the physiological changes of exercise and characteristics of altitude training among children and adolescents at different altitudes. Methods: A total of 235 students aged 7-12 years old living at 3 240 m(Hi group), 300 students at 1 290 m(SubHi), and 300 students living between 1 290 m and 3 240 m (HiSubHi) were selected to perform 20-mSRT test, respiratory function test and heart rate recovery ability evaluation after quantitative load exercise. Results: Performance on 20-mSRT, FVC, FEV1, FEV1% and V% of students aged 7-12 years at different altitudes showed an upward trend with the increase of age. After adjusting for altitudes, there were significant differences in average level of these indicators. Average level of 20-mSRT, FVC, FEV1%, FEV1% and V% in SubHi group were significantly higher than those in Hi group(P<0.05), and the HiSubHi group showed an upward trend compared with Hi group after 1-year adaptation to the sub-highland environment; Except for male FVC (r=0.33, P=0.24) and FEV1 (r=0.34, P=0.22) in HiSubHi group, and the male FEV1% (r=0.46, P=0.14) in SubHi group, all the indicators showed significant association with 20-mSRT(P<0.05); 20-mSRT was only significantly correlated with heart rate recovery ability among SubHi group (male: r=0.78, P<0.05); Female(r=0.81, P=0.01), there was no significant correlation between 20-mSRT and heart rate recovery ability in other groups(P>0.05). Conclusion The results suggest developmental characteristics of aerobic exercise ability, lung function and heart rate recovery ability among 7-12 years old students under different altitudes. The correlation among 20-mSRT, FVC, FEV1, FEV1% and V% might involve high altitude adaptation.

Objective: To explore the correlation between gross motor development and physical activity level of preschool children at different altitudes, so as to provide exercise basis for the development of gross motor ability. Methods: A total of 188 preschool children living in the 3 240 m high plateau (Hi group) in Tashkurgan County, 175 children living in the 1 290 m low plateau (SubHi group) in Kashgar District and 191 children living in the 450 m high plateau (Pla group) in Gaochang District of Turpan were selected as subjects. The children were assessed for gross motor development and tested for physical activity. Results: With the increase of age, the scores of MPA, VPA, MVPA and gross movement of preschoolers in each test group showed an upward trend. The above indexes in SubHi group were significantly higher than those in Hi group at 5 years old, and those in Pla group at 5 years old were significantly higher than those in Hi group ( P <0.05). The level of MPA in SubHi group and Pla group was significantly higher than that in Hi group at 4 years old, and the MVPA in SubHi group at 5 years old was significantly higher than that in Hi group ( P <0.05). SubHi group and Pla group were significantly higher than Hi group at 5 years of age, and Pla group was significantly higher than SubHi group at 4 years of age ( P <0.05). There were no significant differences in the related indexes of gross motor among girls at different altitude groups ( P >0.05). The LPA of the Hi group and the SubHi group was positively correlated with the operational movement score ( r =0.60,0.44), and the LPA of the Pla group was positively correlated with the displacement movement score ( r =0.69).There was a positive correlation between MPA and displacement score of Hi group ( r =0.53), displacement score and gross movement total score of SubHi group ( r =0.45,0.59), and gross movement scores of Pla group ( r =0.69, 0.52 , 0.73). Except the displacement score and gross movement total score of the Pla group, VPA was positively correlated with the gross movement scores of each group ( P <0.05). Conclusion There is a certain correlation between gross motor development and physical activity level in children aged 3-6 years.MVPA can be used as an effective means to improve the development of rough movements of 3-6 year old children.

Objective: To observe the safety and clinical efficacy of tumor antigen-pulsed dendritic cell(DC) vaccine in treatment of advanced malignant tumor.Methods: Ninety-one patients with non-small cell lung cancer,colon and rectal cancer,melanoma,renal carcinoma,breast cancer and other malignant tumors were enrolled in this study.All patients met the selecting standard and signed informed consent.Human dendritic cells were obtained from peripheral blood monocytes by culturing them with granulocyte macrophage-colony stimulating factor and interleukin-4.DC vaccine was prepared from tumor antigen pulsed immature dendritic cells in vitro.Patients received the vaccine therapy once every week and one cycle was defined as once every week for 3 weeks.Results: All the patients received 96 cycles of DC vaccine treatment.Symptoms of toxicity included fever,shivering,aching pain of muscle,asthenia,itching,stifle and transient fatigue;most of the symptoms automatically recovered.Clinical efficacy of the treatment was evaluated in 76 patients.Thirty-one of the 76 patients were stable after treatment and 45 were in progressive situation,with the clinical benefiting rate being 40.8%.Eighty-five patients were followed up.The median time for progression was 2.6 months;the overall survival time was 0.9-30.6 months;and the median survival period was 4.5 months,with the one year survival rate being 9.2%.Conclusion: The results suggest that the DC vaccine therapy is well tolerated in treating patients with advanced malignant tumors and has satisfactory clinical benefit;the clinical value of DC vaccine therapy needs to be further observed.

This study was aimed to investigate the effects of Fomes officinalis polysaccharides (FoP) on exercise-induced fatigue and exercise immune suppression in mice with FoP induced by exercise fatigue and unbalanced exercise immunity.Through treadmill exercise,the long-term exercise immune suppression model fatigue was established.Different doses of FoP were used in the animal experiment.The experimental animal were randomly divided into the positive control group,negative control group,low dose FoP (20 mg· kg-1) + exercise group,middle dose FoP (40 mg· kg-1) + exercise group,and the high dose FoP (80 mg· kg-1) + exercise group.The intragastric administration was given 6 days per week for 8 weeks.Treadmill exercise was administered during these 8 weeks.After 8 weeks,the samples were sacrificed.The levels of malondialdehyde (MDA),superoxide dismutase (SOD),hemoglobin (HB) and creatine kinase (CK) were detected by kit.And the relative expression of IL-4mRNA,INF-γmRNA was detected by RT-PCR.The results showed that compared with the positive control group,FoP can significantly improve the level of HB in mice,the level of serum CK in each FoP dose group was significantly decreased;compared with the positive control group,FoP group can significantly eliminate MDA with no significant difference among different FoP dose groups.The contents of SOD in serum of different dose FoP group and the negative control group were significantly higher than that of the positive control group.The IL-4 mRNA/INF-γ mRNA ratio of different dose FoP group were in basic equilibrium.It was concluded that Uygur FoP can effectively promote the body fatigue recovery,accelerate the clearance of free radicals,and improve the antioxidant ability and immune status.