Objective To explore the clinical significance of detecting platelet parameters in the diagnosis and efficacy-evaluation of adult severe primary immune thrombocytopenia (ITP). Methods Thirty-four adult patients with primary ITP admitted in our hospital served as observa-tion group while another 30 healthy people undergoing physical examination as control group. Platelet parameters on both groups before treatment and in observation group 3and 7 days after treatment were observed.Results Before treatment,observation group was evidently lower in platelet count (PLT)and plateletocrit (PCT)and obviously higher in mean platelet volume (MPV) than control group (P <0.05),but there was no significant difference in platelet distributive width (PDW)(P >0.05).Compared with treatment before,PLT,PCT,MPV increased more markedly 3 d after treatment while PLT,PCT,PDW increased significantly 7 days after treatment in obser-vation group (P <0.05).Conclusion Platelet parameters have important clinical significance in e-valuating the progression and efficacy of adult patients with severe primary ITP.

Objective To explore the clinical significance of detecting platelet parameters in the diagnosis and efficacy-evaluation of adult severe primary immune thrombocytopenia (ITP). Methods Thirty-four adult patients with primary ITP admitted in our hospital served as observa-tion group while another 30 healthy people undergoing physical examination as control group. Platelet parameters on both groups before treatment and in observation group 3and 7 days after treatment were observed.Results Before treatment,observation group was evidently lower in platelet count (PLT)and plateletocrit (PCT)and obviously higher in mean platelet volume (MPV) than control group (P <0.05),but there was no significant difference in platelet distributive width (PDW)(P >0.05).Compared with treatment before,PLT,PCT,MPV increased more markedly 3 d after treatment while PLT,PCT,PDW increased significantly 7 days after treatment in obser-vation group (P <0.05).Conclusion Platelet parameters have important clinical significance in e-valuating the progression and efficacy of adult patients with severe primary ITP.

Objective To analyze the risk factors for extrauterine growth retardation(EUGR)in late preterm infants appropriate for gestational age.Methods The clinical data in 1 402 preterm infants appropri-ate for gestational age delivered and hospitalized in this hospital from January 2016 to June 2022 were analyzed retrospectively.They were divided into the EUGR group(n=244)and the non-EUGR group(n=1 158)ac-cording to whether or not the body weight at discharge was below the 10th percentile of the growth curve for the same gestational age at the same period based on the Fenton's preterm growth curve.The clinical data of preterm infants and mothers of the two groups were collected.The risk factors for EUGR occurrence in pre-mature infants were analyzed.Results Among 1 402 preterm infants appropriate for gestational age,EUGR occurred in 244 cases with the EUGR incidence rate of 17.4％.The EUGR incidence rate had no statistical difference among the different fetal ages of premature infants(P＞0.05).The EUGR incidence rate had sta-tistical difference among different birth weights of premature infants(P＜0.05).The logistic regression anal-ysis showed that male(OR=1.694,95％CI:1.144-2.507),low birth weight(OR=0.989,95％CI:0.988-0.991),feeding intolerance(OR=2.719,95％CI:1.234-5.990),short gestational weeks(OR=0.146,95％CI:0.103-0.207)and hospitalization duration extension(OR=1.073,95％CI:1.031-1.117)were the risk factors for EUGR occurrence in late premature infants appropriate for gestational age in discharge.The sub-group analysis showed that male,low birth weight,feeding intolerance and hospitalization duration extension were the risk factors for EUGR occurrence in the preterm infants with gestational ages of 34-＜36 weeks(P＜0.05).Low birth weight and feeding intolerance only affected the preterm infants≥36 weeks of gesta-tional age(P＜0.05).Conclusion Strengthening the pregnant duration health care and active nutritional sup-port after birth may reduce the risk of EUGR occurrence in late premature infants.

To investigate the effect of hSCGF-alpha on human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs), we obtained hSCGF-alpha using genetic engineering, hSCGF-alpha gene was amplified from hUCMSCs cDNA using two-step PCR and was inserted into pET-28a(+) plasmid vector. Induced by IPTG at 20 degrees Celsius for 24 h, the fusion protein expressed in E. coli BL21 (DE3) was mainly existing in soluble form. The recombinant hSCGF-a was purified using NI-NTA affinity chromatography and the purity was up to 90%. The colony forming test revealed that combined use hSCGF-alpha and rmGM-CSF (recombinant murine GM-colony stimulating factor, rmGM-CSF) had granulocyte/macrophage (GM) promoting effects on murine bone marrow GM progenitor. In addition, the results indicated that hSCGF-alpha and rhGM-CSF had stimulatory effect on hUCMSCs and their synergetic effect was the strongest.
Cell Proliferation ; drug effects ; Cells, Cultured ; Cloning, Molecular ; Drug Synergism ; Escherichia coli ; genetics ; metabolism ; Granulocyte-Macrophage Colony-Stimulating Factor ; pharmacology ; Humans ; Mesenchymal Stromal Cells ; cytology ; metabolism ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; pharmacology ; Stem Cell Factor ; biosynthesis ; genetics ; Umbilical Cord ; cytology