Objective To study the time extended for getting emergency intervention in different modes of transportation and factors influencing the modes of transportation of patients with ST elevation myocardial infarction (STEMI).Methods A total of 564 consecutive patients with STEMI admitted from September 2013 to June 2016 were enrolled in the study.The clinical data about time consumed for getting emergency intervention and modes of transportation were collected.Results According to the mode of transportation,patients were divided into three groups:emergency care system (EMS) transportation group (n =96),self-transportation group (n =206) and referral group in which the patients were sent in from other hospitals (n =262).EMS transportation group had significantly shorter total ischemic time before emergency treatment than self-transportation group (229 rin vs.418 min,P ＜ 0.05) and referral group (229 min vs.512 rin,P ＜ 0.05),and significantly shorter length of pre-hospital time than self-arrival group (55 min vs.110 min;P＜0.05) and referral group (55 min vs.372 min;P＜0,05).The referral group had longer pre-hospital time and the self-transportation group had longer door-to-balloon time,but there was no difference in total ischemic time between the self-arrival and referral group (Z =-1.882,P =0.068).Multivariate logistic regression was used to analyze influence factors in mode of transportation:(1) patients characterized with high school or university education,profession of civil service,and their transportation distance more than 30 km were greater in number than referral group (P ＜ 0.05);(2) patients identified with senior middle school education,staff member of public sectors or company,their transportation distance less than 30 km,and with killip grade above Ⅱ were more likely to have EMS transport (P ＜ 0.05);(3) patients defined as businessmen without taking out new rural cooperative medical insurance,taking up transportation distance less than 80 km,and subjecting to killip grade Ⅰ had a higher proportion of individuals of this kind taking self-transportation (P ＜ 0.05).Conclusion Mode of transportation is an important factor that affects the time extended to get emergency intervention.Education level,occupation,medical insurance type,transportation distance,killip grade are associated with modes of transport.

Objective To explore the clinical significance of detecting platelet parameters in the diagnosis and efficacy-evaluation of adult severe primary immune thrombocytopenia (ITP). Methods Thirty-four adult patients with primary ITP admitted in our hospital served as observa-tion group while another 30 healthy people undergoing physical examination as control group. Platelet parameters on both groups before treatment and in observation group 3and 7 days after treatment were observed.Results Before treatment,observation group was evidently lower in platelet count (PLT)and plateletocrit (PCT)and obviously higher in mean platelet volume (MPV) than control group (P <0.05),but there was no significant difference in platelet distributive width (PDW)(P >0.05).Compared with treatment before,PLT,PCT,MPV increased more markedly 3 d after treatment while PLT,PCT,PDW increased significantly 7 days after treatment in obser-vation group (P <0.05).Conclusion Platelet parameters have important clinical significance in e-valuating the progression and efficacy of adult patients with severe primary ITP.

Objective To explore the clinical significance of detecting platelet parameters in the diagnosis and efficacy-evaluation of adult severe primary immune thrombocytopenia (ITP). Methods Thirty-four adult patients with primary ITP admitted in our hospital served as observa-tion group while another 30 healthy people undergoing physical examination as control group. Platelet parameters on both groups before treatment and in observation group 3and 7 days after treatment were observed.Results Before treatment,observation group was evidently lower in platelet count (PLT)and plateletocrit (PCT)and obviously higher in mean platelet volume (MPV) than control group (P <0.05),but there was no significant difference in platelet distributive width (PDW)(P >0.05).Compared with treatment before,PLT,PCT,MPV increased more markedly 3 d after treatment while PLT,PCT,PDW increased significantly 7 days after treatment in obser-vation group (P <0.05).Conclusion Platelet parameters have important clinical significance in e-valuating the progression and efficacy of adult patients with severe primary ITP.

Objective? To expLore the status quo and infLuencing factors of comfort LeveLs in patients receiving chemotherapy after surgery for coLorectaL cancer. Methods? TotaLLy 150 patients who received chemotherapy after surgery for coLorectaL cancer in a CLassⅢ Grade A hospitaL in a city from January 2017 to February 2018 were seLected as convenient sampLing and investigated with the generaL information questionnaire, Chemotherapy Patient Comfort ScaLe and SociaL Support Rating ScaLe (SSRS). Univariate anaLysis, Pearson correLation anaLysis and muLtivariate Linear regression anaLysis were used to expLore the factors affecting the patients' comfort LeveLs. ResuLts? The comfort LeveL of these patients with coLorectaL cancer after surgery totaLed (72.36±9.34). Comfort LeveLs were positiveLy correLated with sociaL support (P＜ 0.05). MuLtivariate Linear regression anaLysis showed that educationaL background, enterostomy received or not, compLication, treatment protocoL and sociaL support were the main factors affecting the patients' comfort LeveL. ConcLusions? The comfort LeveL of patients receiving chemotherapy after surgery for coLorectaL cancer stands at a medium LeveL. MedicaL and nursing workers shouLd improve sociaL support for the patients and take more targeted interventions based on the patients' actuaL situation to improve their comfort LeveLs and quaLity of Life.

To investigate the effect of hSCGF-alpha on human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs), we obtained hSCGF-alpha using genetic engineering, hSCGF-alpha gene was amplified from hUCMSCs cDNA using two-step PCR and was inserted into pET-28a(+) plasmid vector. Induced by IPTG at 20 degrees Celsius for 24 h, the fusion protein expressed in E. coli BL21 (DE3) was mainly existing in soluble form. The recombinant hSCGF-a was purified using NI-NTA affinity chromatography and the purity was up to 90%. The colony forming test revealed that combined use hSCGF-alpha and rmGM-CSF (recombinant murine GM-colony stimulating factor, rmGM-CSF) had granulocyte/macrophage (GM) promoting effects on murine bone marrow GM progenitor. In addition, the results indicated that hSCGF-alpha and rhGM-CSF had stimulatory effect on hUCMSCs and their synergetic effect was the strongest.
Cell Proliferation ; drug effects ; Cells, Cultured ; Cloning, Molecular ; Drug Synergism ; Escherichia coli ; genetics ; metabolism ; Granulocyte-Macrophage Colony-Stimulating Factor ; pharmacology ; Humans ; Mesenchymal Stromal Cells ; cytology ; metabolism ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; pharmacology ; Stem Cell Factor ; biosynthesis ; genetics ; Umbilical Cord ; cytology

Objective To investigate the delay of door to signature time in primary percutaneous coronary intervention (PCI) and its influence in patients with ST segment elevation myocardial infarction (STEMI),therefore to provide a scientific basis for further effective shortening the time of primary PCI in patients with STEMI.Methods A total of 226 patients who diagnosed with STEMI and underwent primary PCI at Henan Provincial People's Hospital from June 2016 to December 2017 were enrolled in the study.Observation indicators include:(1) baseline data of patients;(2) time segments in primary PCI:total ischemic time (TIT),door to balloon time (DTBT),door-to-signature time (DTST),signature to balloon time (STBT);(3) the demographic characteristics of the family members who signed informed consent;and (4) the psychological factors and coping strategies of family members before signing informed consent.All data was analyzed using SPSS software (version 22.0).Multiple linear regression analysis was used to analyze the influencing factors of delay of DTST.A P＜0.05 was considered statistically significant.Results In this study,226 patients with STEMI who were first diagnosed in our hospital had a mean age of 55.23±10.80 years,and 181 (80.1％) were male.The median of TIT,DTBT,DTST,STBT were 312 min,166 min,82 min,and 80 min.The ratio of DTST in DTBT and TIT was 50％ and 28.5％,respectively.The multiple linear regression analysis showed that the number of direct family members (P＜0.001),the degree of educational in middle school and below (P=0.010),high school/technical secondary school (P=0.029),families worrying about the high cost of medical care (P=0.020),families consulted each other repeatedly (P=0.022),and consulted the other medical staff(P=0.022) are risk factors of DTST delay,and city residence (P=0.048) is the protection factor of DTST delay.Conclusions The long time of DTS is a reality of the practice of primary PCI in China.The factors that lead to longer DTST include demographic characteristics,psychological factors and coping strategies of family members.The STBT of primary PCI in China should be taken into the value while emphasizing the DTBT.

Objective To explore the risk factors for postoperative recurrence in patients with refractory epilepsy and establish a predictive model.Methods Clinical materials of 280 refractory epilepsy patients with surgical treatment in the hospital from June 2021 to October 2022 were retro-spectively collected,with a follow-up of one year after surgery.These patients were divided into non-recurrence group with 238 cases and recurrence group with 42 cases according to their recurrence sta-tus.The risk factors for postoperative recurrence in refractory epilepsy patients were analyzed by uni-variate and multivariate Logistic regression analyses;the receiver operating characteristic(ROC)curve was drawn to evaluate the predictive value of the model for postoperative recurrence in refractory epilepsy patients.Results Multivariate Logistic regression analysis showed that disease duration ex-ceeding 5 years,incomplete concordance between the preoperative localization of the lesion and the-surgical site,low serum vitamin B6 level,and high level of serum monocyte chemoattractant protein-1(MCP-1)were the significant risk factors for postoperative recurrence in refractory epilepsy patients(OR=2.705,2.314,1.790 and 2.284,P＜0.05).A regression model was built based on these findings,and an ROC curve for predicting postoperative recurrence in refractory epilepsy patients was plotted based on the predicted probability logit(P).When logit(P)exceeded 14.52,the area under the curve(AUC)was 0.850,with a sensitivity of 78.57％and a specificity of 80.67％.Conclusion In refractory epilepsy patients with surgical treatment,disease duration exceeding 5 years,incomplete concordance between the preoperative localization of the lesion and the surgical site,low serum vita-min B6 level,and elevated MCP-1 level are identified as risk factors for postoperative recurrence.The established regression model for predicting postoperative recurrence in refractory epilepsy pa-tients demonstrates a high predictive value,and can be utilized to identify populations with high-risk of recurrence and guide targeted interventions to reduce the risk of recurrence.

Objective To explore the risk factors for postoperative recurrence in patients with refractory epilepsy and establish a predictive model.Methods Clinical materials of 280 refractory epilepsy patients with surgical treatment in the hospital from June 2021 to October 2022 were retro-spectively collected,with a follow-up of one year after surgery.These patients were divided into non-recurrence group with 238 cases and recurrence group with 42 cases according to their recurrence sta-tus.The risk factors for postoperative recurrence in refractory epilepsy patients were analyzed by uni-variate and multivariate Logistic regression analyses;the receiver operating characteristic(ROC)curve was drawn to evaluate the predictive value of the model for postoperative recurrence in refractory epilepsy patients.Results Multivariate Logistic regression analysis showed that disease duration ex-ceeding 5 years,incomplete concordance between the preoperative localization of the lesion and the-surgical site,low serum vitamin B6 level,and high level of serum monocyte chemoattractant protein-1(MCP-1)were the significant risk factors for postoperative recurrence in refractory epilepsy patients(OR=2.705,2.314,1.790 and 2.284,P＜0.05).A regression model was built based on these findings,and an ROC curve for predicting postoperative recurrence in refractory epilepsy patients was plotted based on the predicted probability logit(P).When logit(P)exceeded 14.52,the area under the curve(AUC)was 0.850,with a sensitivity of 78.57％and a specificity of 80.67％.Conclusion In refractory epilepsy patients with surgical treatment,disease duration exceeding 5 years,incomplete concordance between the preoperative localization of the lesion and the surgical site,low serum vita-min B6 level,and elevated MCP-1 level are identified as risk factors for postoperative recurrence.The established regression model for predicting postoperative recurrence in refractory epilepsy pa-tients demonstrates a high predictive value,and can be utilized to identify populations with high-risk of recurrence and guide targeted interventions to reduce the risk of recurrence.