Objective To observe the age-related changes of brain-derived neurotrophic factor(BDNF) and investigate the effects of ginsenosides(GS) on BDNF in nucleus basalis of Meynert(NBM) and cerebral cortex of aged rats. Methods Twenty-four female Wistar rats were randomly divided into 3 groups: young group(3-5 months),aged groups(27 months) and GS group(27 months).GS group was fed with GS from 18 to 27 months.Immunohistochemistry and Imaging analysis were used to show the expression and distribution of BDNF in NBM and cerebral cortex of each group. Results BDNF level of aged group was much lower than that of young group in these two brain areas(P

Objective To design the family support scale for adolescent patients with nephritic syndrome. Methods 192 adolescent patients with nephritic syndrome were selected in the pilot investigation, and then formed the first questionnaire. The reliability and the validity of the questionnaire were evaluated by the experts. Results The questionnaire involved 15 items and its reliability and the validity were satisfied. There was a positive correlation between the family support and the self-nursing degree. Conclusion The reliability and the validity of this questionnaire are both up to the demands, it can used to study the condition of family support for adolescent patients with nephrotic syndrome.

AIM:To investigate the effects of myeloid-related protein 8/14 ( MRP8/14 ) on the survival and apoptosis of human alveolar epithelial cell line A549, and to explore the role of nuclear factor-κB (NF-κB) in this process. METHODS:A549 cells were treated with different doses of MRP8/14 or stimulated with MRP8/14 for different time.The effect of MRP8/14 on the viability of A549 cells was determined by CCK-8 assay.The apoptotic rates were tested by flow cytometry.The nuclear translocation of NF-κB p65 was detected by Western blot and indirect immunofluorescence.Be-sides, the phosphorylation level of NF-κB p65 was determined by Western blot.NF-κB-specific inhibitor Bay 11-7082 was used to further analyze the role of NF-κB in the apoptosis induced by MRP8/14.RESULTS:The viability of the A549 cells was affected by MRP8/14 in a dose-and time-dependent manner.Flow cytometric analysis showed that the apoptotic rates were increased in the cells treated with MRP8/14 (P<0.01).In A549 cells administered with MRP8/14, NF-κB p65 was significantly phosphorylated and translocated into the nuclei, suggesting the activation of NF-κB signaling pathway. However, NF-κB-specific inhibitor Bay 11-7082 significantly attenuated the cell apoptosis induced by MRP8/14 ( P <0.01).CONCLUSION:NF-κB plays an important role in regulating the apoptosis of human alveolar epithelial cells in-duced by MRP8/14.

Aim To study effect of the extract of Fagopynum cymosum(Fr4) on the apoptosis and telomeraseactivity in human leukemia HL-60 cells.Methods HL-60 cells were treated with Fr4 and inhibition of proliferation was measured with MTT assay.Cell morphology before and after the treatment was examined with light and electron microscopy.FILC-Annexin-V and PI double staining was used to detect cell membrane-mediated apoptosis by FCM.DNA fragmentations were analyzed with DNA gel electrophoresis.Furthermore,telomerase activity was determined with PCR-ELISA-based telomeric repeat amplification(TRAP).Results The proliferation of HL-60 cells was inhibited by Fr4 with an IC_(50) of 115 mg?L~(-1).Typical morphology and biochemical feature of apoptosis was observed with Fr4 60～240 mg?L~(-1).The percentages of early apoptosis cells were increased in a dose-dependent manner.Telomerase activity was decreased in a dose-dependent manner during the process of apoptosis.Conclusion Fr4 induced apoptosis in HL-60 cells.Down-regulation of the telomerase activity in HL-60 cells might be contributed to Fr4-induced apoptosis.

Objective To observe therapeutic effects of HuGanJieXian decoction on rats hepatic fibrosis induced by tetrachloride. Methods Rat models of hepatic fibrosis were constructed by intraperitoneal injection of tetrachloride.HuGanJieXian decoction composed of low, middle, and high dose curcumin were given to these rats respectively at the same time. Sho-saiko-to compound treatment group and Fufangbiejiarangan Tablets treatment group were made as positive control groups. After twelve weeks, all rats were executed. Serum samples were kept for measuring serum levels of PC-Ⅲ, LN, and HA. Left livers were extirpated for pathologic examination including H.E and Masson stainings. Grade of hepatic fibrosis were evaluated according to SSS system. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) of supematant centrifugated from hepatic tissue homogenate were detected. Results Serum levels of PC-Ⅲ, LN, and HA were depressed obviously in decoction groups compared with those of fibrotic group (P<0.05) , especially in the low-dose curcumin group.HuGanJieXian Decoction could increase the level of SOD and decrease the level of MDA (P<0.05) , especially in the low-dose curcumin group. Staining of H. E and Masson showed that degrees of hepatic fibrosis in decoction groups were improved obviously compared with that of the fibrotic group. Conclusion HuGanJieXian Decoction can improve rat hepatic fibrosis, the mechanism of this effect may be associated with protecting hepatic cell membrane and anti- peroxidative damage.

Objective To investigate the mental health states of health system staffs in a district of Chongqing municipality. Methods Seven hundred and seventy-three health system staffs were taken as the research subjects for conducting the investigation by the Symptoms Checklist-90 (SCL-90).With the total SCL-90 score >160 as the positive psychological symptom,the total mean scores of SCL-90 and the 9 items of factor score of somatization,obsession,personal relations,depression,anxiety,hostility,dread, paranoid and psychotics were performed the statistical analysis.Results The positive rate of psychological symptoms in 773 health system staffs was 17.46% (135/773),among them which in the clinical nurses,clinicians and nonclinical staffs were 23.20%(58/250),15.63%(50/320)and 13.30%(27/203)respectively.The somatization and obsession scores of the health system staffs in this district were obviously higher than those of the national norm (P <0.05),while their personal relations and paranoid scores were obviously lower than those of the national norm(P <0.01).The paranoid scores of the staffs in the district health units were higher than those of the staffs worked in town health units(P <0.05),and other 8 items of factor score and the total mean scores were significantly higher than those in the town health unit staffs (P <0.01).The scores of obsession,depression,anxiety,hostility and the total mean scores of the clinical nurses were significantly higher than those of the clinicians (P <0.01),while scores of so-matization,personal relations,paranoid and psychotics had no significant differences between them(P > 0.05).The total mean scores of the clinical nurses were higher than those of the non-clinical staffs(P <0.05).Conclusion The scores of multiple factors had significant differences between the health system staffs in this district and the national norm.The psychological health status in the clinicians was poorer than that in the non-clinical staffs,especially the psychological health status in the clinical nurses were much poorer.

Objective:To explore the clinical effect of ulnar artery conjoined perforator flap for repair of multiple finger damage.Methods:12 cases of multiple finger injuries were repaired by ulnar artery conjoined perforator flap from March, 2011 to October, 2017 in our hospital, with 4 finger damages in 2 cases, 3 finger damage in 4 cases, 2 finger damage in 6 cases. The position of soft tissue injury were from the palm to the fingertip and the phalanx defect were from proximal to the fingertip. The total area of skin defect was (135.6±12.3) cm 2 (6.0 cm×16.0 cm to 6.0 cm×35.0 cm). The total area of skin flaps was (143.5±11.2) cm 2 (5.0 cm ×20.0 cm to 3.2 cm×47.0 cm) (bilateral forearm). The perforator flaps of bilateral forearm ulnar artery were used in 5 cases and unilateral in 7 cases. All flaps had more than 2 perforator pedicles. The flaps were designed as long strips and wrapped in wounded phalanges by spiral winding. The perforator artery of the flap was anatomized with the proper digital artery or dorsal metacarpal artery, the accompanying vein was anatomized with the corresponding digital palmary or dorsal metacarpal vein, the superficial vein of the flap was anatomized with the corresponding dorsal digital vein or dorsal metacarpal vein, and the nerve of the flap was anastomosed with the corresponding proper digital nerve or dorsal metacarpal corresponding sensory nerve. The donor site was sutured directly except for 1 case of skin graft, and the curative effect was observed after operation. The follow-up included skin flap sensation, appearance, blood circulation, bone absorption and hand movement function, daily life, recovery of work, etc. The evaluation standard is the upper limb function evaluation trial standard of Hand Surgery Society of Chinese Medical Association. Results:All flaps survived. One case had skin necrosis of about 1.5 cm×1.5 cm at the end of the flaps, which was repaired by second-stage suture. All cases were followed up for 6 months to 6.5 years, with an average of 16.7 months. The flaps had good texture, no pigmentation, no swelling, no scar or abrasion on the fingertips, and the two-point discrimination was 6-10 mm, with an average of 8.6 mm. The incidence of bone obsorbtion was 59.4%(19/32) and the mean finger shortening was 0.8 cm. Among them, iliac bone grafting was performed in 5 cases and 6 fingers. The function of holding and pinching of the injured finger was partly restored, there was no significant effect on daily life. The average grip strength of the injured hand reached 60.3% of the healthy side. Evaluation of hand function according to the trial standard of replantation function of severed fingers of Hand Surgery Society of Chinese Medical Association. The motor function of the affected hand was excellent in 2 cases, good in 5 cases, poor in 4 cases and had in 1 case. The excellent and good rate was 58.3%(7/12). The appearance of donor site is acceptable.Conclusions:The ulnar artery conjoined perforator flap provides a useful way of thinking and effective operation for repair of patients with multiple finger damage or degloving injury.

Objective:To explore the clinical effect of ulnar artery conjoined perforator flap for repair of multiple finger damage.Methods:12 cases of multiple finger injuries were repaired by ulnar artery conjoined perforator flap from March, 2011 to October, 2017 in our hospital, with 4 finger damages in 2 cases, 3 finger damage in 4 cases, 2 finger damage in 6 cases. The position of soft tissue injury were from the palm to the fingertip and the phalanx defect were from proximal to the fingertip. The total area of skin defect was (135.6±12.3) cm 2 (6.0 cm×16.0 cm to 6.0 cm×35.0 cm). The total area of skin flaps was (143.5±11.2) cm 2 (5.0 cm ×20.0 cm to 3.2 cm×47.0 cm) (bilateral forearm). The perforator flaps of bilateral forearm ulnar artery were used in 5 cases and unilateral in 7 cases. All flaps had more than 2 perforator pedicles. The flaps were designed as long strips and wrapped in wounded phalanges by spiral winding. The perforator artery of the flap was anatomized with the proper digital artery or dorsal metacarpal artery, the accompanying vein was anatomized with the corresponding digital palmary or dorsal metacarpal vein, the superficial vein of the flap was anatomized with the corresponding dorsal digital vein or dorsal metacarpal vein, and the nerve of the flap was anastomosed with the corresponding proper digital nerve or dorsal metacarpal corresponding sensory nerve. The donor site was sutured directly except for 1 case of skin graft, and the curative effect was observed after operation. The follow-up included skin flap sensation, appearance, blood circulation, bone absorption and hand movement function, daily life, recovery of work, etc. The evaluation standard is the upper limb function evaluation trial standard of Hand Surgery Society of Chinese Medical Association. Results:All flaps survived. One case had skin necrosis of about 1.5 cm×1.5 cm at the end of the flaps, which was repaired by second-stage suture. All cases were followed up for 6 months to 6.5 years, with an average of 16.7 months. The flaps had good texture, no pigmentation, no swelling, no scar or abrasion on the fingertips, and the two-point discrimination was 6-10 mm, with an average of 8.6 mm. The incidence of bone obsorbtion was 59.4%(19/32) and the mean finger shortening was 0.8 cm. Among them, iliac bone grafting was performed in 5 cases and 6 fingers. The function of holding and pinching of the injured finger was partly restored, there was no significant effect on daily life. The average grip strength of the injured hand reached 60.3% of the healthy side. Evaluation of hand function according to the trial standard of replantation function of severed fingers of Hand Surgery Society of Chinese Medical Association. The motor function of the affected hand was excellent in 2 cases, good in 5 cases, poor in 4 cases and had in 1 case. The excellent and good rate was 58.3%(7/12). The appearance of donor site is acceptable.Conclusions:The ulnar artery conjoined perforator flap provides a useful way of thinking and effective operation for repair of patients with multiple finger damage or degloving injury.

Objective:To explore the potential categories of resourcefulness in primary caregivers of stroke patients and analyze the differences in the characteristics of different types of caregivers, so as to provide basis for clinical construction of effective intervention plans.Methods:The 308 primary caregivers of stroke patients who met the research standards in the Department of Neurology of General Hospital of Ningxia Medical University, People′s Hospital of Ningxia Hui Autonomous Region, the First People′s Hospital of Yinchuan from December 2020 to August 2021 were selected as the research objects by convenient sampling method.The general information questionnaire, Resourcefulness Scale and Social Support Revalued Scale were used for cross-sectional investigation.Results:The resourcefulness of the primary caregivers of stroke patients were divided into 2 potential categories: low resourcefulness group (62.7%, 193/308) and high resourcefulness group (37.3%, 115/308). Uni-variate analysis showed that there were differences between potential categories: age and education level of stroke patients, whether there was long-term medication history, number of other chronic diseases, had there been any complications due to illness, age, education level and occupation of caregivers, average daily care duration, family relations, and social support ( χ2 values were -3.34 - 23.62, all P<0.05). Logistic regression analysis showed that social support and education level of caregivers were the influencing factors of caregiver resourcefulness ( P<0.05). Conclusions:The resourcefulness in the primary caregivers of stroke patients has certain characteristics. Clinically, targeted intervention measures should be formulated according to the different types of caregiver resourcefulness characteristics of caregivers and their influencing factors to improve the caregiver resourcefulness of stroke patients.

Objective:To investigate the clinical effect of fingertip reconstruction using the toenail osteocutaneous flap without vascular anastomosis.Methods:From January 2015 to October 2020, the clinical data of patients with fingertip injuries were analyzed retrospectively, which were treated with the toenail osteocutaneous flap without vascular anastomosis in Chang’an Xin’an Hospital. The donor site was closed primarily or covered by a local flap. The appearance and function of the patients’ fingers were followed up. All cases were evaluated by the standard of thumb finger reconstruction function evaluation of the Chinese Medical Association of Hand Surgery. Appearance and function of nail bed after reconstruction was evaluated from four aspects.Results:A total of 11 cases (12 fingers) were enrolled, including eight males and three females. The average age was 35.6 years (range, 17-50 years). The etiologies included crush-related injury, cutting injury, mangled injury, and chemical corrosion injury. The injuries were found in thumb, index finger, middle finger, ring finger, or combined with multiple fingers. All fingertip defects were located distal to the nail root, including the defects distal to lunula in four cases, the defects distal to middle of the nail in five fingers , and the defects distal to 1/3 of the nail in three cases. Elective surgery was performed in 7 cases and emergency surgery in 4 cases. The defects ranged from 0.2 cm/0.5 cm/0.5 cm to 0.6 cm/0.8 cm/1.0 cm (phalanx/nail/finger pulp). The size of the toenail osteocutaneous flap ranged from 0.2 cm/0.5 cm/0.7 cm to 0.6 cm/0.8 cm/1.0 cm (phalanx/nail/flap). The defects length of the finger pulp were less than 1.0 cm. All reconstructed fingers survived, and the follow-up was 6-30 months (average, 12.5 months). The appearances of the reconstructed fingers were pulmp and ruddy, and the nails were flat. There was no dysfunction in the donor site. The average healing time of bone was 2.1 months (range, 1.5-3.0 months). According to the evaluation of index function, seven cases were excellent, four cases were good. The appearance and function of the nail bed were excellent in six fingers, good in five fingers, and poor in one finger.Conclusion:It is a simple and effective way to reconstruct the fingertip using the toenail osteocutaneous flap without vascular anastomosis.