1.Development and application of a surgical information acquisition system based on performance assessment
Hui HUANG ; Haihua SHAN ; Jian CHEN
Chinese Journal of Hospital Administration 2017;33(8):605-607
Covered in the paper is the development of the hospital's surgical information acquisition system during the healthcare reform and upgrading of its performance assessment scheme.Such a system is designed to precisely collect surgery scheduling, daytime surgery, and outpatient surgery workload, conducive to developing a performance assessment scheme based on surgery workload, motivating medical workers, and improving quality of care.
2.Study on Extraction Technique of Sidiming Capsule
Jianchun HUANG ; Renbin HUANG ; Zheng YANG ; Haihua TANG ; Weizhe JIANG
China Pharmacy 2007;0(33):-
OBJECTIVE:To optimize the extraction technique for Sidiming capsules.METHODS:The technical conditions for the water decoction and the alcohol precipitation were optimized respectively by the orthogonal experiment design L9(34)with hydrosoluble extract used as the index for the water decoction and the catalpol extract for alcohol precipitation.The content of Catalpol was determined by HPLC.RESULTS:The optimum conditions were as follows:decocting the crude drugs twice with 8-fold water,1 h each time.The physic liquor extracted by water was filtered,mixed and concentrated to 1.0 g?mL-1(crude drug),and then precipitated by 75% concentration of alcohol for 24 h.Then the physic liquor was filtered,concentrated and dried by microwave vacuum concentration dryer to obtain the dry ointment.CONCLUSION:The optimum extraction procedure is stable and reliable,and it can be used as the optimal extraction procedure of Sidiming capsules.
3.ERK1/2 pathway mediates the protective effect of hydrogen sulfide against cisplatin-induced injury in human marrow mesenchymal stem cells
Haihua GUO ; Gang HUANG ; Jingchun LI ; Jianqiang FENG ; Bicheng YONG
Chinese Journal of Orthopaedics 2014;34(3):323-329
Objective To explore the protection and mechanism of hydrogen sulfide (H2S) preconditioning against injury induced by cisplatin in human marrow mesenchymal stem cells (HMMSCs).Methods HMMSCs were treated by cisplatin at 0,5,10,20,40,60,80 mg/L concentrations for 24 h respectively and were exposed to cisplatin at 20 mg/L concentrations for 0,6,12,24,36,48 h respectively.HMMSCs were pretreated with NaHS at 0,0.4,0.6,0.8,1.0 mmol/L respectively for 30 min before exposed to cisplatin at 20 mg/L concentrations for 24 h.HMMSCs were treated by U0126 or combined with human epidermal growth factor (HEGF) together for 30 min before they were preconditioned with NaHS for 30 min.The cell survival rate,cell apoptosis rate,the expression of p-ERK1/2 and t-ERK1/2 were recorded.Results The cell survival rate decreased to 71.72%±2.72%,59.41%±5.44%,50.37%±4.55%,38.97%±2.92%,30.11%±4.64% and 21.71%±5.35% respectively after cells were treated with cisplatin at 5,10,20,40,60,80 mg/L concentrations respectively,and the differences were statistically significant compared with 0 mg/L group.The cell viability fell to 70.30%±6.20%,61.63%±2.70%,51.29%±3.13%,38.72%±3.66% and 27.57%±2.32% after HMMSCs were treated with cisplatin at 20 mg/L for 6,12,24,36,48 h respectively,and the differences were statistically significant compared with 0 h group.The cell viability increased to 65.99%±2.67%,72.93%±5.44%,75.10%±4.71% and 76.56%± 5.25% when HMMSCs got pretreatment of NaHS,and the differences had statistical significance compared with cisplatin group.The cell apoptotic rate decreased from 35.29%±2.77% to 18.62%±0.97% when HMMSCs were pretreated with NaHS at 0.6 mmo/L.Treatment of HMMSCs with cisplatin at 20 mg/L for 24 h reduced p-ERK1/2 expression.The pretreatment of NaHS could inhibit the inhibitory action to the expression of p-ERK1/2 induced by cisplatin.Pretreatment with U0126 or HEGF inhibited or promoted the protection and the upregulated expression ofp-ERK1/2 caused by NaHS pretreatment.Conclusion The preconditioning of H2S can protect cell damage caused by cisplatin via activating the ERK1/2 pathway of HMMSCs.
4.Clinical application of retrograde medial and lateral gastrocnemius muscle flap for repairing the soft tissue defects of the middle and lower third of the leg
Zhi PENG ; Zhiyuan WU ; Haihua HUANG ; Xiaorui GUO ; Zhenhua JIA
Chinese Journal of Microsurgery 2010;33(4):274-277,后插二
Objective To explore clinical application of retrograde medial and lateral gastrocnemius muscle flap for soft tissue defects of the middle and lower third of the leg. Methods From August 2008 to December 2009, in our hospital we adopted retrograde medial and lateral gastrocnemius muscle flap to renovate 5 cases of refractory soft tissue defects of the middle and lower third of the leg. Results Five cases of retrograde medial gastrocnemius muscle flap were survived, morphology and function of soft tissue defects were renovated well. Conclusion This operation is an effective and reliable technique for soft tissue defects of the middle and lower third of the leg, which is performed without sacrificing the major blood vessels, probing vascular pedicle and matching vascular anastomosis.
5.Fentanyl induced hyperalgesia and upregulation of pro-inflammatory cytokines in dorsal root ganglions in ;rats
Lu CHANG ; Fang YE ; Haihua SHU ; Lin YANG ; Wenqi HUANG
The Journal of Practical Medicine 2016;32(12):1912-1915
Objective To investigate the expression of pro-inflammatory cytokines in lumbar dorsal root ganglions (DRG) of rats model of high-dose fentanyl induced hyperalgesia. Methods 64 male SD rats were divided into 2 groups (n = 32), fentanyl group and normal saline (NS) group. The rats were injected with fentanyl (60 μg/kg) or NS 4 times in total subcutaneously with a 15-minute interval. Mechanical and thermal nociception were measured via the tail pressure test (tail flick thresholds, TFT) and paw withdrawal test (paw withdrawal latency, PWL) at 1 day before, at 1, 2, 3 and 4 hour and on 1 ~ 7 day after administration. 4 rats were sacrificed and the lumbar DRG were harvested to analyze the expression of PGE2 , IL-1β, IL-6 and TNF-αvia ELISA. Results There were no significant changes of TFT, PWL and the expression of pro-inflammatory cytokines in DRG compared to baseline of rats in NS group. The value of TFT , PWL in fentanyl group were above the baseline at the 1 ~ 4 hour and below the baseline at 1~3 day after fentanyl injections. PGE2 , IL-1β, TNF-α and IL-6 increased on 1,3,5,7 day after fentanyl injections significantly. Conclusions High-dose fentanyl induced significant hyperalgesia and up-regulation of pro-inflammatory cytokines in DRG. The expression pro-inflammatory cytokines peaked later and were more protracted than the change of behavior test and show no direct relationship between the two.
6.Revision of the Chinese-version of the individual adaptability measure and its application and analysis in college freshmen
Ning LI ; Yaqiong HOU ; Haihua LIU ; Zheng HUANG
Chinese Journal of Behavioral Medicine and Brain Science 2015;24(2):181-184
Objective To revise the individual adaptability measure (I-ADAPT-M) developed by Ployhart & Bliese,and analyze the reliability and validity of its application in college freshmen.Methods Revise the I-ADAPT-M,One thousand and seven hundred freshmen were asked to complete the test,one month later,two hundred and seventy-seven freshmen were simple randomly chosen to report their General Life Satisfaction.Then,the data were analysis by SPSS 18.0 and Amos 4.0.Results The exploratory factor analysis showed that,the Chineseversion of the I-ADAPT-M consisted of 23 projects and 4 dimensions (crisis and creativity adaptability,interpersonal adaptability,stress tolerance adaptability,and resistant learning adaptability),which could account for over 45.344% of total variance.The Cronbacha coefficient of the Chinese-version of the I-ADAPT-M was 0.82.The confirmatory factor analyzed further revealed the structure of four-dimensional scale(x2=708.80,x2/df =3.16,CFI =0.95,NNFI =0.94,RMSEA =0.051,SRMR =0.053).Male had significant high core in crisis and creativity adaptability and resistant learning adaptability than female.Female had significant high core in interpersonal adaptability than male.Colleges-leader had significant high core in 4 dimensions than general students,it suggested that the Chinese-version of the I-ADAPT-M had good discriminant validity.General Life Satisfaction of the subjects had significant positive correlation with crisis and creativity adaptability,stress tolerance adaptability and interpersonal adaptability,had little correlation with resistant learning adaptability.Conclusion The Chinese-version of the I-A-DAPT-M has good reliability and validity to measure the individual adaptability of the freshman.
7.GLP-1 regulates proliferation, differentiation and apoptosis of endothelial progenitor cells isolated from human umbilical cord blood by targeting the SDF-1/CXCR4 signaling pathway
Feng LIU ; Wenqiong XU ; Na MIN ; Jiazhen TANG ; Haihua HUANG
Tianjin Medical Journal 2015;43(5):457-460
Objective To investigate the molecular regulatory mechanism of glucagon like peptide 1 (GLP-1) on proliferation, differentiation and apoptosis of human umbilical cord blood endothelial progenitor cells (EPCs). Methods EPCs were isolated from the umbilical cord blood of healthy pregnant women and cultured in 6-hole cell plate at 2×105 density in vitro, transfected with empty vector plasmid (control group), pcDNA3-GLP-1 plasmid (GLP-1 group), pcDNA3-GLP-1plasmid+AMD3100 (GLP-1+AMD3100 group) and simple AMD3100 (AMD3100 group). The pcDNA3-GLP-1 was transfected into EPCs. The 25μmol/L AMD3100 was used to block the SDF-1/CXCR4 signal pathway of EPCs for 1 h. The cell proliferation was determined by MTT method. The mRNA expressions of differentiation and apoptosis related genes PPARγ, C/EBPα and Caspase-3 were investigated by RT-PCR, and Caspase-3 activity was determined by Caspase-3 activity assay kit. Results Compared to control group, AMD3100 inhibitor showed no effects on cell proliferation, differentiation and apoptosis, while over-expression of GLP-1 in EPCs obviously promoted cell proliferation, and differentiation related genes PPARγand C/EBPαmRNA expression, but down-regulated mRNA expression and the activity of Caspase-3 significantly (P<0.05), indicating that GLP-1 increased proliferation and differentiation of EPCs while decreased cell apoptosis. When the SDF-1/CXCR4 signaling pathway was blocked by AMD3100, over-expression of GLP-1 induced promotion of cell proliferation, and the differentiation was decreased significantly and the apoptosis was significantly increased (P<0.05). Conclusion These data confirm that GLP-1 might promote EPCs proliferation and differentiation, and inhibit cell apoptosis through the regulation of the SDF-1/CXCR4 signaling pathway.
8.The Inhibitant Effects Of Parecoxib, A Cyclooxygenase-2 Inhibitor, in Acute Fentanyl Induced Hyperalgesia in Rats
Haihua SHU ; Qiaobo LI ; Fang YE ; Wenqi HUANG
The Journal of Practical Medicine 2015;(5):711-714
Objective To investigate the inhibitant effects of parecoxib, a cyclooxygenase-2 (COX-2) inhibitor, in acute fentanyl induced hyperalgesia in rats. Methods (1) Thirty SD rats (n=6 for each group) were subcutaneously injected with fentanyl (40 μg/kg × 4 times with a 15 min-interval) or saline to establish acute fentanyl induced hyperalgesia model, andparecoxib (5, 10 mg/kg) was administrated intraperitoneally in parecoxib group. Mechanical nociceptive thresholds were measured by the tail pressure test every hour during 1~4 hours and once a day during 1~5 days. (2) 24 SD rats (n = 6 foreach group) were subcutaneously injected with fentanyl as above described and randomly administrated intraperitoneally with parecoxib in 10 mg/kg in 15 min before and at the 4th hour and the 1st day after fentanyl injection except rats in the control group, mechanical nociceptive thresholds were measured by the tail pressure test at time points as above described. Results (1)Acute high dose fentanyl injection induced mechanical hyperalgesia and parecoxib (at 5 or 10 mg/kg)inhibited fentanyl induced hyperalgesia in rats. (2)Parecoxib inhibited fentanyl induced hyperalgesia at 15 min before and at the 4th hour after, but not on the 1st day after fentanyl injection. Conclusions This study provides the first evidence that subanalgesia doses of parecoxib had inhibitory effects on acute fentanyl induced hyperalgesia in time-dependent manners in rats.
9.Long-term efficacy observation of donepezil in the treatment of Alzheimer's disease
Haihua HUANG ; Mingqiu LI ; Gaoxuan JIANG ; Xin MU ; Qinghong CHEN
Chinese Journal of Geriatrics 2012;31(2):98-101
Objective To evaluate the long-term efficacy and safety of donepezil in treating patients with Alzheimer's disease(AD).Methods Totally 86 patients with AD were randomly divided into control group(n =43)and treatment group(n =43).The control grou,p received conventional therapy with aniracetam,nimoldipine and ginkgo tablet,while the study group was administrated with donepezil(10 mg/d)on the basis of conventional therapy.The improvements of recognitive ability,mental state,activities of daily life were graded by mini-mental state examination (MMSE),Alzheimer's disease assessment scale-cog(ADAS-cog),activity of daily living(ADL)and global deterioration scale(GDS).The scores were compared between the groups before the treatment and 3,6,12,18,24,30,36,42,48,54,60,66 and 72 months after the treatment,respectively.Results The scores of MMSE,ADAS-cog and GDS after 3 months and ADL score after 6 months (t=2.361,-2.198,-1.790,-2.420,P<0.05 or P<0.01)were improved in treatment group than in control group with the best effects at 12 months(all P<0.01)and the scores continued to decrease after 36 months.At 72 months,the score improvements in treatment group were 7.5 for MMSE,20.3 for ADAS-cog,19.5 for ADL,and 1.4 for GDS as compared with control group(all P <0.01).In contrast to pretreatment,there were statistically significant differences in the scores of MMSE,ADAS-cog and GDS at 3,6,12,18 and 24 months,and in the score of ADL at 6,12,18,24 and 30 months after treatment(P<0.05 or P<0.01).The differences in the scores of ADAS-cog and GDS after 24 months as well as MMSE and ADL after 30 months were not found(P>0.05)between pre-treatment and post-treatment.Conclusions Donepezil might be long term effective and safe in slowing down the recognitive and overall function deterioration of AD.
10.The function of Fas, FasL and Bcl- 2 in the pathogenesis of autoimmune thyroid disorders
Shenren CHEN ; Zhichao ZHENG ; Yiping LUO ; Lijuan DENG ; Haihua HUANG ; Linxing CHEN ; Wei ZHANG
Chinese Journal of Pathophysiology 1986;0(03):-
AIM: To investigate the expression and function of apoptosis-related protein, Fas, FasL, and Bcl-2 in the pathogenesis of autoimmune thyroiditis. METHODS: Immunohistochemical staining was performed on 20 Hashimoto's thyroiditis (HT), 20 Graves' disease (GD), and 20 thyroid follicular adenoma (TFA, as control). RESULTS: All the cases expressed Fas, mainly on the cell surface and cytoplasm. FasL was found in all except 3 of the TFA. Bcl-2 in 15 of HT, 19 of GD, 17 of TFA. In TFA follicular cells expressed moderate Fas and minimal or absent FasL. In HT, follicles adjacent to infiltrating lymphocytes showed a increased levels of Fas and FasL, but infiltrating lymphocytes exhibited weaker staining of Fas and FasL than thyrocytes. In GD, thyrocytes and lymphocytes showed nearly similar Fas with HT, but rather weaker for FasL than HT. Bcl-2 was nearly similar in GD and TFA, but follicular cells in vicinity of lymphocytes and lymphocytes located in germinal centers of HT tissues exhibited significantly weaker. CONCLUSION: The expression of Fas, FasL and Bcl-2 in Hashimoto's thyroiditis and Graves' disease was nearly similar. Strong FasL expression and weak Bcl-2 expression on the follicles in HT may induce apoptosis. These results provide further proof that the functions of Fas and its ligand and Bcl-2 may play an important part in the pathogenesis of autoimmune thyroid diseases. The lymphocytes do not seem to be directly engaged in the process with their own FasL, but they may provide some cytokines that, in turn, up-regulates Fas and/or FasL leading to apoptosis.