1.Comparison of the clinicopathological features between synchronous multiple early gastric cancer and single early gastric cancer
Guangxia CHEN ; Chuannan WU ; Haihan ZHANG ; Liyong GAO ; Jin DING ; Shiyu LIU ; Guoxin ZHANG
Chinese Journal of Digestion 2018;38(12):806-810
Objective To investigate the clinicopathological features and correlation between synchronous multiple early gastric cancer (SMEGC)and single early gastric cancer (EGC).Methods From January 2008 to December 2016,the clinical data of 994 patients with EGC who underwent open or laparoscopic gastrectomy surgery were collected from the electronic medical data base of the First Affiliated Hospital of Nanjing Medical University and Xuzhou No.1 People's Hospital.The data of patients including gender,age,tumor morphologys,tumor location,tumor size,histological type,depth of invasion,lymph nodes metastasis,lymphovascular metastasis,peripheral nerve invasion,and blood types were analyzed.T test and Chi square test were used for statistical analysis.Results Among 994 EGC patients,27 cases (2.7%) were SMEGC,and 967 cases (97.3%) were single EGC.The percentage of male and female of single EGC were 71.4% (690/967) and 28.6% (277/967),respectively;the percentage of male and female of SMEGC were 88.9% (24/27) and 11.1% (3/27),respectively,and there was statistically significant difference in the gender composition ratio between single EGC and SMEGC (x2 =3.975,P=0.046).The incidence of ulcer in single EGC and SMEGC were 50.6% (489/ 967) and 29.6 % (8/27),respectively,and the difference in the incidence of ulcers between single EGC and SMEGC was statistically significant (x2 =4.653,P=0.031).There were no statistically significant differences between single EGC and SMEGC in gross morphology,depth of invasion,lymph nodes metastasis,lymphovascular metastasis,peripheral nerve invasion,tumor location,pathological type and blood types (all P>0.05).In the SMEGC patients,the incidence of main lesions invading the mucosa was 48.1% (13/27) and submucosa invasion was 51.9% (14/27);and for minor lesions,the corresponding incidences were 77.8% (21/27) and 22.2% (6/27),respectively,and the difference was statistically significant (x2 =5.063,P<0.05).There were no statistically significant differences between the main lesions and minor lesions in tumor size,pathological type,with or without ulcers,gross morphology and tumor location (all P>0.05).Conclusions The main risk factors of SMEGC are male and no ulcerative lesions.The clinicopathological features are similar between main lesions and minor lesions in SMEGC.
2.Research progress in healing mechanism and treatment approach for tendon-bone interface injury
Ziyun LI ; Xiulin WU ; Haihan GAO ; Yamin LI ; Jia JIANG ; Jinzhong ZHAO
Chinese Journal of Trauma 2022;38(7):646-652
Tendons and bones are connected at the tendon-bone interface to transmit force and exchange biological information. However, the formation of fibrous scars after injury to the tendon-bone interface makes it difficult to recover the original structure during surgery and thus reduces its performance. Therefore, the healing of the tendon-bone interface is a hotspot in sports medicine. Numerous studies have already demonstrated that a variety of molecules and cells participate in the tendon-bone interface reconstruction process, and yet the specific mechanism remains unclear. At present, a great number of studies have been carried out on treatment methods, but clinical treatment are varied with no unification. Therefore, the authors review the advances in the biology and mechanics of healing mechanisms of tendon-bone interface as well as the main methods promoting tendon-bone interface healing, so as to provide references and new ideas for further researches on tendon-bone interface healing.
3.Effects of thyroxine and propylthiouracil on nitric oxide synthase expression in human umbilical vein endothelial cells
Haihan JIA ; Miao JING ; Chang LIU ; Yunyan GAO ; Lanchun LIU ; Peng LIU
Chinese Journal of Endemiology 2020;39(3):168-171
Objective:To investigate the effects of thyroxine (T 4) and propylthiouracil (PTU) on nitric oxide synthase expression in human umbilical vein endothelial cells (HUVEC cells). Methods:HUVEC cells were cultured in vitro, the experiment was divided into 6 groups: control (without T 4 and PTU), 10 -9, 10 -7, 10 -4 mol/L T 4, PTU (5 μg/ml PTU), and 10 -4 mol/L T 4 + PTU, the action time was 24 h. CCK-8 method was used to detect cell activity; nitrate reductase method was used to detect nitric oxide (NO), total nitric oxide synthase (TNOS) and endothelial nitric oxide synthase (eNOS) contents; Western blotting was used to detect eNOS protein expression level. Results:The differences of cell survival rates [(100.00 ± 0.00)%, (96.73 ± 1.17)%, (86.20 ± 7.54)%, (47.37 ± 9.10)%, (53.37 ± 5.47)%, (53.40 ± 8.84)%] among the 6 groups were statistically significant ( F = 29.42, P < 0.05). Compared with the control group, the cell survival rates of 10 -7, 10 -4 mol/L T 4, PTU, and 10 -4 mol/L T 4 + PTU groups were significantly reduced ( P < 0.05). There were statistically significant differences in the contents of NO and TNOS among the 6 groups ( F = 3.93, 3.46, P < 0.05). Compared with the control group, the TNOS content in PTU group was significantly reduced ( P < 0.05); compared with the 10 -4 mol/L T 4 group, the NO contents in PTU and 10 -4 mol/L T 4 + PTU groups were significantly reduced ( P < 0.05). There were no significant differences in eNOS content and protein expression level among the 6 groups ( F = 0.24, 0.17, P > 0.05). Conclusions:High concentration of T 4 can cause damage to the activity of HUVEC cells cultured in vitro, and PTU can alleviate it by regulating NO and TNOS. The specific mechanism of action still needs to be further studied in molecular biological experiments.
4. Clinical study on the relationship between hyperthyroidism and vascular endothelial injury
Manli CHANG ; Tianlong YU ; Miao JING ; Yunyan GAO ; Chang LIU ; Lanchun LIU ; Xiaohui SU ; Haihan JIA ; Peng LIU
Chinese Journal of Endemiology 2019;38(11):918-921
Objective:
To observe the relationship between hyperthyroidism and vascular endothelial injury.
Methods:
From 2016 to 2017, data of 60 patients with simple hyperthyroidism, autoimmune thyroid disease, cardiovascular and cerebrovascular diseases were collected from the Second Affiliated Hospital of Harbin Medical University. And 60 healthy subjects were enrolled in the same period as a healthycontrol group. The enzyme-linked immunosorbent assay (ELISA) was used to detect the content of vascular endothelial injury markers (vWF), thrombomodulin (TM), endothelin-1 (ET-1), P-selectin in serum of all subjects, as well as the serum thyroid stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), anti-thyroglobulin antibody (TgAb), and anti-thyroid peroxidase antibody (TPOAb) in patients with cardiovascular and cerebrovascular diseases levels. The correlation analysis of vascular endothelial injury in the simple hyperthyroidism group, autoimmune thyroid disease group, cardiovascular and cerebrovascular diseases group and healthy control group was conducted.
Results:
There were statistically significant differences between the healthy control group, the simple hyperthyroidism group, the autoimmune thyroid disease group and the cardiovascular and cerebrovascular diseases group in the serum levels of vWF, TM, ET-1 and P-selectin, respectively(