Objective To compare the effect of laparoscopic-assisted and open distal gastric cancer radical operation for advanced gastric cancer and evaluate the feasibility and short-term outcomes of laparoscopic-assisted distal gastric cancer radical operation.Methods The clinical data of 198 patients from January 2007 to December 2011 were analyzed retrospectively.Ninety-one patients who underwent laparoscopic-assisted distal gastric cancer radical operation was in laparoscopy group,and 107 patients who underwent open distal gastric cancer radical operation was in open operation group.Results The operative time in laparoscopy group was significantly longer than that in open operation group [(260.08 ± 26.28) min vs.(231.74 ± 17.33) min],and intraoperative blood loss volume,incision length,recovery of bowel activity,time of stay in hospital and recovery eating were significantly shorter than those in open operation group [(152.13 ± 51.05) nl vs.(348.00 ± 110.36) ml,(5.02 ± 0.82) cm vs.(19.13 ± 1.57) cm,(2.79 ± 0.75) d vs.(4.49 ± 1.09) d,(9.97 ± 2.08) d vs.(12.15 ± 2.46)d,(6.91 ± 1.44) d vs.(7.45 ± 1.08) d,P＜ 0.05].The total number of lymph nodes and postoperative complication had no significant difference between two groups (P ＞ 0.05).Conclusions The laparoscopic-assisted distal gastric cancer radical operation for advanced gastric cancer is safe and feasible.It has better short-term outcomes.

Objective To explore the clinical effect of Xuebijing injection in the treatment of paraquat poi-soning.Methods 67 patients with acute paraquat poisoning were divided randomly into the treatment group ( 35 cases) and control group(32 cases),the control group were given conventional therapy,and the treatment group were given Xuebijing 100mL intravenous injection additionally based on conventional therapy,twice per day for 1-2 week. The biochemical indexes of organs injury and mortality in the two groups were detected.Results Compared with the control group,the serum Cr,ALT,AST,CRP and CK level were lower and the PaO2 was higher in treatment group,the differences were significantly(all P<0.01).The fatality rate of the treatment group was 20.0%,which was lower than 34.4%of the control group(P<0.05).Conclusion Xuebijing injection could relieve the organ damage of paraquat poisoning,decrease the mortality,which has a therapeutic effect for treatment of acute paraquat poisoning.

Immune thrombocytopenia (ITP), the most common hemorrhagic disease, is an organ-specific autoimmune disease characterized by decreased platelets count due to auto-antibodies mediating platelets destruction and insufficient platelets production. The etiology of ITP is still not completely known. Regulatory T cells, also called Tregs, are character-ized by CD4+CD25+, and positive of transcription factor forkhead box P3. They belong to a subpopulation of T cells special-ized for immune regulation and play an important role in maintaining the immune balance. Decreased production and defect-ed function of CD4+CD25+Treg was found not only in the ITP animal model but also in the ITP patients. It indicates that the Treg was involved in the pathology of ITP. This review focus on the characteristic and function of Tregs and their relationship with pathogenesis of ITP.

Objective To investigate hemodynamie change in uremia complicating heart failure of elderly patients. Methods Totally 241 cases with uremia complicating heart failure received measurement of circulation dynamic detection(CD).The volume load,myocardial contractility and blood pressure were analyzed in the elderly group aged(71±6)years (n=116) and young and middleaged group aged(38± 10)years(n =125). Results The central venous pressure(CVP)[(26.7±11.3)cmH2O vs.(23.6 ± 10.7) cm H2O,t=2.410,P =0.010],effective circulation volume (ECV)[(4362± 1340)ml vs.(3085 ± 1001 ) ml,t =7.674,P =0.000],stroke volume(SV) [(185 ± 52) ml vs.(114±41)ml,t=7.803,P=0.000],cardiac output (CO)[( 13.9±4.3)L/min vs.(11.2±3.6)L/min,t=4.802,P=0.000],left ventricular end diastolic volume (LVD)[(388± 145)ml vs.(258±98)ml,t =7.673,P =0.000],left ventricular end systolic volume ( LSV ) [( 223 ± 95 ) ml vs.( 135 ± 59) ml,t =7.186,P=0.000] were significantly higher group than in young and middle-aged group.The ejection pressure (EP)[(178±29)mm Hg vs.(183±24)mm Hg,t=2.323,P=0.012],myocardium negative inotropic(MNC) [(0.609±0.149)vs.(0.683±0.188),t=3.113,P=0.002],ejection fraction (EF) [(0.433± 0.034) vs.(0.445 ± 0.031 ),t =2.451,P =0.010],diastolic blood pressure (DBP) [( 87 ±14)mm Hg vs.(1.08±22)mm Hg,t=8.141,P=0.000],systematic vascular resistance(SSR)[(952±207)gcm-4 s-2 vs.(1217± 308) gcm-4 s-2,t =7.143,P=0.000],ejection resistence(ER) [( 178± 29)vs.( 183 ± 24),t =2.323,P =0.012] were lower in elderly group than in young and middle-aged group.DBP(x2 =16.474,P=0.000,OR=0.752,95％CI:0.714-0.790),SBP(x2 =11.913,P=0.000,OR=1.148,95％CI:1.091-1.205),ER(x2 =17.892,P=0.000,OR=0.906,95％CI:0.861-0.951),CVP(x2 =14.672,P=0.000,OR=0.698,95％CI:0.663-0.733) and LDV(x2 =21.080,P=0.000,OR=0.942.95％ CI:0.895-0.989) were dangerous factors of uremia complicating heart failure.Conclnsions The increased volume load,decreased myocardial contractility and cardiac afterload may appear in the elderly patients with uremia complicating heart failure.

This study was aimed to evaluate the applicability of common Chinese word segmentation software used in the literature study of traditional Chinese medicine (TCM) text,in order to put forward ideas on developing specialized TCM text word segmentation software.By means of installing and operating Chinese word segmentation software,the text segmentation experiment was conducted on TCM text samples.Aspects,such Chinese word segmentation accuracy,speed,maneuverability,reliability,extendibility,portability and other characteristics,were compared among different Chinese word segmentation software.The results showed that there were differences on the accuracy,speed,maneuverability,reliability,extendibility,portability among different Chinese word segmentation software.It was difficult to achieve best performance on different aspects by single software.Through the comparison of different Chinese word segmentation software,the Pan-Gu Segment software showed the best performance on accuracy,with good maneuverability,and high word segmentation efficiency,which was the most suitable for word segmentation in TCM text.It was concluded that developing specialized TCM text segmentation software may be the best solution to meet the requirement of text segmentation in TCM literature study.Basic studies should be strengthened from aspects,such as the construction of standard TCM copus,the completion of TCM dictionary base,the introduction,optimization and innovation of word segmentation algorithm,as well as the development of word segmentation software for TCM text.

Objective: To observe the synergetic effect of folic acid and genistein on apoptosis in the early stage of neuron and its possible mechanism. Methods: Cyclophosphamide was added to primary cultured neurons to induce apoptosis. Three different dosages of folic acid and genistein were used to inhibit apoptosis. The results were observed by flow cytometry and scanning electron microscopy (SEM). Results: 1.Folic acid and genistein might play a role in early stages of neuron apoptosis, and the synergetic effect was demonstrated when folic acid and genistein were administered together. 2. The membrane changes in early stages of neuron apoptosis observed by scanning electron microscope were also in agreement with the flow cytometry data. Conclusion: Genistein might reinforce the protective effect of folic acid on neural tube defects by restraining the apoptosis of the early stage of neuron.

Objectives:To investigate the changes of portal hemodynamics in patients with liver cirrhosis and its clinical value. Methods:Forty patients with liver cirrhosis and twenty healthy volunteers as controls were involved in this study.The max diameters,mean flow velocity,flow rate and congestion index(CI) of the portal vein trunks(PV) and splenic veins(SV) were detected by Color Doppler Ultrasound in all subjects. Results:The PV diameters(PVD), PVT flow rate(PVF), PVCI and SV diameter(SVD),SV flow rate(SVF),SVCI were significantly highter in patients than in controls( P

Antineoplastic Agents ; therapeutic use ; Enhancer of Zeste Homolog 2 Protein ; Epigenesis, Genetic ; Histone Deacetylase Inhibitors ; therapeutic use ; Humans ; Hydroxamic Acids ; therapeutic use ; Indoles ; therapeutic use ; Lymphoma ; classification ; drug therapy ; genetics ; metabolism ; MicroRNAs ; metabolism ; Phenotype ; Polycomb Repressive Complex 2 ; metabolism ; Polycomb-Group Proteins ; metabolism

Objective To study the ways of auto swivel reposition under Barium meal for treating stomach volvulus and its clincial value.Methods The patients with stomach volvulus confirmed by gastroenterography underwent reposition according to the volvulus and the effect was observed.The cause was examined.Results 100 cases underwent treatment of auto swivel reposition under Barium meal,among whom 98 cases were successfully repositioned and 51 cases were known for the cause.Conclusion Auto swivel reposition under Barium meal for treating stomach volvulus and its clincial value is simple and non-invasive and acceptable,being of great value.

BACKGROUND: Extract of astragalus mongholicus has good effects on antioxidization and oxygen free radical scavenge. Bone marrow mesenchymal stem cells (BMSCs) have multiple directional differentiation potential and superiority of autologous transplantation. This can provide a new way for treating neurodegenerative diseases.OBJECTIVE: To explore the differentiation of BMSCs of rats following astragalus mongholicus induction.DESIGN: A cytological in vitro study.MATERIALS: One clean male rats aged 6 weeks were purchased from Experimental Animal Center of Chinese Academy of Medical Sciences. Astragalus mongholicus injection (lot number 060105) were obtained from Dali Pharmaceutical Co., Ltd.METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10<'8>/L were incubated in 12-well plate with a coverslip. After 80% 90% cells were confluent, the medium was changed. BMSCs were then incubated in DMEM, supplemented with 200 g/L astragalus mongholicus injection and 15% fetal bovine serum, for 6 days. BMSCs in the control group were not treated with astragalus mongholicus injection.MAIN OUTCOME MEASURES: Morphological changes in BMSCs were observed under the inverted microscope before and after induct.ion. Expression of specific markers was determined by immunocytochemical staining following induction.RESULTS: Primary cultured BMSCs were round. At the fourth passage, BMSCs were spindle. BMSCs after induction showed processes, which became more with prolonged time. Some processes were branch-shaped, forming a network structure. Results of immunocytochemical staining demonstrated that many cells were positive for nestin, neuron specific enolase and glial fibrillary acidic protein on day 3, and abundant cells were positive for microtubule-associated protein-2 on day 6.CONCLUSION: Astragalus mongholicus induces differentiation of BMSCs into neural stem cells, and then promotes the differentiation into neurons or glial cells, and makes differentiated cells mature and aging.