Objective To understand the distribution and antimicrobial resistance of pathogens isolated from bone and joint infection sites of orthopedic patients,so as to provide reference for prevention and therapy of bone and joint infection.Methods 114 specimens from bone and joint infection sites of orthopedic patients between June 2010 and October 2013 were surveyed,pathogens and antimicrobial susceptibility of pathogens were analyzed.Results A total of 145 pathogenic isolates were obtained,62.07%(n=90)of which were gram-negative bacteria,34.48%(n=50)were gram-positive bacteria,2.07%(n=3)were Candida spp .,and 1 .38%(n =2)were anaerobic bacteria. The top 4 pathogens were Pseudomonas aeruginosa (20.69%),Staphylococcus aureus (12.41 %),Escherichia coli (11 .03 %),and Enterobacter cloacae (9.65%).These pathogens presented remarkable resistance.The resistant rate of Pseudomonas aeruginosa to ceftazidime was up to 40.00%;the resistant rates of Escherichia coli to com-pound sulfamethoxazole,ciprofloxacin and levofloxacin were all 100.00%.Conclusion Gram-negative bacteria are the major pathogens in orthopedic ward,the isolated strains are resistant to multiple antimicrobial agents.

Objective To evaluate the expression of immunohistochemistry markers p53, p16 and Ki-67 in penile cancer and penile intraepithelial neoplasia (PIN). Methods 33 patients who suffered penile cancer and received organ-sparing surgery from Jan 2004 to Dec 2013 were retrospectively analysed. The expression of p53, p16 and Ki-67 were detected by EnVision/HRP two steps immunohistochemistry. Results The positive expression rates of p53 in normal penile epithelium, low-grate penile intraepithelial neoplasia, high-grate penile intraepithelial neoplasia, well-differentiated squamous cell carcinoma and poor-differentiated squamous cell carcinoma were 0, 16.7 %, 38.5 %, 30.0 % and 66.7 % respectively. The expression rates of p16 were 20.0 %, 50.0 %, 53.8 %, 70.0 % and 33.3 % respectively. The expression rates of Ki-67 were 0, 16.7 %, 61.5 %, 65.0 % and 75.0 % respectively. Conclusions The combined detection of p16 and Ki-67 can contribute to discover PIN and penile carcinoma much more earlier. p53 may be related to the occurrence and development of low differentiated squamous cell carcinoma, and p16 may be related to the occurrence and development of well-differentiated squamous cell carcinoma.

Objective To investigate the effect of ginsenoside on degeneration of learning and memory in aged mice and the mechanism. Method Eighty female C57BL/6J mice aged 20-mo were randomly divided into control group and three ginsenoside treatment groups at dosage of 25,50,100 mg/(kg bw?d) respectively by drinking. In addition, 20 female C57BL/6J mice aged 3 mo were used to be young control group. Eight mo later, the learning and memory abilities of the mice were checked by Morris water maze. Thereafter, the mice were killed by decapitation,and their serum was collected to determine the level of SOD, GSH-Px and MDA. Hippocampal morphology was examined by Nissl stain; and the expression of brain-derived neurotrophic factor (BDNF) in hippocampus was studied using Western blot method. Results 50,100 mg/(kg bw?d) ginsenosides administration could significantly shorten the escape latency of the aged mice in Morris water maze. Furthermore,the alleviated oxidative stress and up-regulated expression of BDNF were observed in 50, 100 (mg/kg?d) ginsenosides groups compared with aged control group. Serum level of GSH-Px was higher in 25 (mg/kg bw?d) ginsenosides group compared to that of aged control group. The number of Nissl-positive cell had no significant difference between all groups. Conclusion 50,100 mg/(kg bw?d) ginsenoside can significantly delay the degeneration of learning and memory in aged mice by lowering oxidative damage and up-regulating BDNF expression in hippocampus.

Objective: To establish a determination for psoralen and isopsoralen in Longbishu Capsules (Fructus Psoraleae, Herba Leonuri, Succinum, Pseudobulbus Cremastrae seu pleiones, Herba Lysimachiae, Spora Lygodii). Methods : TLC-Scanning was used. Results : The average recovery of psoralen was 97.4% and RSD was 3.06%,respectively, and isopsoralen was 97.6% and RSD was 2.72%,respectively. Conclusion : The method can be used for quality control of Longbishu Capsules.

Compared to ligand-binding assay( LBA) , liquid chromatography coupled to tandem mass spectrometry( LC-MS/MS) methods could improve specificity, reduce the time of method development and enhance efficiency for quantitation of monoclonal antibodies( mAb) .This review summarizes the application of LC-MS/MS assays to the quantitation of mAb in order to facilitate the research of quantitation of mAb in drug development.

Objective To investigate the expression of p38 MAPK gene and p38 MAPK protein,and explore the effect of p38 MAPK in inflammation reaction of severe acute pancreatitis.Methods 48 cases with severe acute pancreatitis were chosen as research group,and another 48 healthy cases as control group.The expression of p38 MAPK gene were investigated by RT-PCR,and the expression of p38 MAPK and P-p38 MAPK protein were investigated by western blot. Results The expression of p38 MAPK mRNA,p38 MAPK protein and P-p38 MAPK protein in research group were higher than that in control group,the differences were all statistically significant(P<0.05 ).Conclusion The expression of p38 MAPK gene and p38 MAPK protein were significantly increased in patients with severe acute pancreatitis,which showed that p38 MAPK played an important role in inflammation reaction.

Objective To investigate the diagnostic criteria, clinical management and prognostic factors of primary central nervous system (CNS) histiocytic sarcoma (HS). Methods An adult patients with primary CNS HS was reported, and literature on the rare entity were reviewed. Results The patient had no previous history of disease. Magnetic resonance imaging (including magnetic resonance spectroscopy) suggested a mass in the frontal lobe with obvious vasogenic edema, which was considered glioma. Surgery was the initial treatment and the tumor was totally removed. The histological findings revealed the HS. The patient received concomitant chemoradiotherapy postoperatively and currently lived without recurrence. Conclusions Primary CNS HS is extremely rare hematopoietic malignant tumor. The pathological feature and comprehensive immunophenotype panel are the useful ways to establish the diagnosis till now. Primary CNS HS should be treated intensively with surgery, chemotherapy, and/or radiation therapy. However, prognosis is disappointed in most of patients. Concomitant chemoradiotherapy might be an alternative treatment.

OBJECTIVE To investigate infection of Chlamydia trachomatis(CT) and Ureaplasma urealyticum(Uu) in patients with nongonococcal urethritis(NGU). METHODS C.trachomatis was determined by fast immune method of antigen-antibody.U.urealyticum was detected by liquid culture medium. RESULTS Among 320 cases the positive rate of C.trachomatis,and U.urealyticum was 36.25% and 43.75%,respectively.and that of complicated infection was 20.00%.There were significant differences between men and women in C.trachomatis and U.urealyticum infection(P

Objective: To explore the depression stares of the elderly experienced the Wenchuan earthquake and to know the related factors. Methods: Using the convenient sampling method, 390 elderly living in Chengdu and Deyang City were included in this investigation. Demographic data was recorded and the Center for Epidemiolog-ic Studies Depression (CES-D) was applied individually. Results: In this sample, 9.8% of the elderly had sus-pected depression symptom and 30.3% had depression symptom. Depression status was related to sex, education,habitual residence, occupation and experiences in earthquake. Compared with the elderly who were males, with middle school education degree, without horrible experiences in the past, with less fearful/helpless/horrible feel-ings in earthquake, the elderly who were females (OR=2.03), with education degree below primary school (OR =2.59), with horrible experiences in the past (OR=2.06), and with strong fearful/helpless/horrible feeling in earthquake (OR=1.59), had more serious depression. Conclusion: After 8 months of Wenchuan earthquake, lo-cal elderly victims are still in severe depression. The elderly who are female, with low education level, engaged in farm work, suffered great loss should be paid special attention.

Objective To study the effect of Schwann cell enclosed by antibody of neural cell adhesion molecule L1 on injured spinal cord. Methods Over 98% of the purity of Schwann cells obtained from bilateral sciatic nerves of 2 days newborn SD rats, the concentration of Schwann cells was about 2.5?104 /?l. The Schwann cells were enclosed by the antibody of neural cell adhesion molecule L1 using co-culture. The adult SD rats (weight 200-250 g) were used to establish the model of spinal cord injury by hemi-transection at the left side of T10 level. The animals were divided into three groups; the SC group was transplanted with 20 ?l suspension Schwann cells; the anti-L1 group with 20 ?l Schwann cells enclosed by antibody of neural cell adhesion molecule L1; and the control group was injected solely with normal saline to the injured cord. Eight weeks later regenerated neural axons were investigated through horseradish peroxiase HRP retrograde trace immunohistochemistry of neurofilament and Western blot. Results Few regenerated neural axons appeared in the control group; some of regenerated neural axons could be observed in anti-L1 group; plentiful and bulky regenerated neural axons were found in SC group. The group with antibody had significant less HRP positive neurons and neural axons than the group without antibody. Western blot showed that the quantity of neurofilament in the anti-L1 group was only two thirds of the SC group. Conclusion Schwann cell-derived neural cell adhesion molecule L1 is able to enhance the neural axon regeneration of injured spinal cord.