1.Study on correlation between calsification of ligamentum nuchae and degeneration of ligamentum flavon
Changfeng WANG ; Lianshun JIA ; Haifeng WEI
Orthopedic Journal of China 2006;0(03):-
[Objective]To study the relationship between ossification of ligamentum nuchae(LN) and degeneration of ligamentum flavon(LF) of cervical spondylotic myelopathy(CSM).[Method]Fifty-six cervical LF samples from CSM patient of C_(3~7) in relation to the stage of ossification of LN samples(experimental group,EG) and 38 samples of corresponding ligament from patient with cervical vertebral trauma(CVT)(control group,CG) were obtained.Thickness of LF was measured,their pathological change was examined by microscopy,content of collagen and kydrolyproline and the ratio of type Ⅰ/ type Ⅱ collagen were determined by Woessners method and setting Salting out method.The correlation among the thickness of degenerative LF,the ratio of type Ⅰ/ type Ⅱ collagen,the content of collagen and hydrokyproline,and arrangement disorder of LF in relation to the ossification stage of LN were analyzed.[Result]In EG,as compared with in CG,there showed a decrease of elastic fibre,decrease of ratio type Ⅰ/ type Ⅱ collagen due to significant increase of type Ⅱ collagen,increase collagen content and disorder arrangement of LF.In relation to the ossification of LN,above changes were more marked in LF of C_(4、5),C_(5、6),than that of C_(3、4),C_(6、7)(P
2.Advances in the research of anti-CD20 therapeutic monoclonal antibodies.
Chenglian DENG ; Jia ZOU ; Haifeng SONG
Acta Pharmaceutica Sinica 2013;48(10):1515-20
As targeted drugs to B-cell malignancies, anti-CD20 monoclonal antibodies have been proved to be important in therapeutic antibody field. With three generations in more than ten years' development, the structures of these drugs have been improved, and many new indications have been found. Nowadays, these kinds of antibodies are not only used in the treatment of lymphoid malignancies, but also been proved to be useful in some autoimmune diseases treatment, and their new indications are still being expanded. With the optimization of their clinical dosage regimens, drug reaction has been increased, thus, therapeutic and side effects of anti-CD20 monoclonal antibody have been further improved as well. However, the exact mechanism of action of their combination therapy with other chemical drugs is still unclear, which remains to be further studied. This article reviewed new development of anti-CD20 therapeutic monoclonal antibodies research in recent years.
3.Nuclear magnetic resonance-based metabonomics features before and after 800 meters race
Zhi LI ; Haifeng MA ; Ying WU ; Yinhao JIA
Chinese Journal of Tissue Engineering Research 2015;(46):7484-7491
BACKGROUND:Metabolic characteristics of the 800 meters race is not very clear, which creates a lot of obstacles for the exercise training of 800 meters race. OBJECTIVE: To investigate the characteristics of material and energy metabolism of 800 meters race, to improve the scientific training of the 800 meters race and to verify the feasibility of nuclear magnetic resonance-based urine metabonomics in sports training monitoring. METHODS: Seven middle distance athletes were subject to an 800 meters race at ultimate intensity, and their urine metabonomics analysis was conducted in combination with the Human Metabolism Database and other databases before and after exercise. RESULTS AND CONCLUSION:OPLS-DA model showed that the metabolic samples before and after movement presented the cluster-like distribution. The change was mainly caused by the folowing metabolites, including leucine, lactic acid, creatinine, succinic acid, hypoxanthine, and adenosine. Through the analysis we could infer that the 800 meters race is a sport event mainly dependent on glycolysis for energy supply, working together with raw phosphate and aerobic oxidation for energy supply. The amino acid metabolism and methane metabolism of the athletes were more vigorous after 800 meters running, and moreover, there were certain oxidative stress during the 800 meters race. These findings indicate that nuclear magnetic resonance-based urine metabonomics takes the advantages of invasive, high sensitivity, less sample requirement, simple processing.
4.Effects of glutamine pretreatment on intestinal ischemia-reperfusion inju-ry in rats
Aili WANG ; Qiong NIU ; Chengxia LIU ; Xingfang JIA ; Haifeng LIAN
Chinese Journal of Pathophysiology 2014;(9):1703-1707
AIM:To determine the effects of glutamine ( Gln) pretreatment on intestinal ischemia-reperfusion (I/R) injury in the rats.METHODS: Thirty male Wistar rats were randomly divided into 3 groups (n=10): sham group, I/R group and Gln pretreatment group .The rats in Gln pretreatment group were pretreated with 1 g· kg -1 · d-1 Gln by orogastric route for 7 d, the rats in the other 2 groups were pretreated with normal saline .Intestinal I/R was induced by 30-min occlusion of the superior mesenteric artery followed by 24 h of reperfusion .After the operation , the plasma endo-toxin, serum D-lactic acid, superoxide dismutase ( SOD) and malondialdehyde ( MDA) levels were measured .The intesti-nal mucosal injury was observed with HE staining and evaluated using Chiu 's scoring.RESULTS: Serum D-lactic acid, endotoxin level , MDA level and Chiu's score in I/R group were significantly higher than those in sham group and Gln group (all P<0.05).Serum SOD activity was significantly lower than that in sham group and Gln group (P<0.05).CON-CLUSION:Glutamine has a protective effect on the intestines during ischemia-reperfusion injury .The mechanism may be related to oxidative stress response .
5.Comparison of Two Quantitative Assays for Determination of Rh-anti-CD20 zumab and Their Application to Pharmacokinetic Study
Chenglian DENG ; Jia ZOU ; Lun OU ; Lihou DONG ; Haifeng SONG
Chinese Journal of Analytical Chemistry 2014;(3):337-342
An enzyme linked immunosorbent assay ( ELISA ) and a flow cytometry assay ( FCA ) based on Wil2-S cells were developed and systematically compared for quantification of recombinant anti-CD20 humanized monoclonal antibody ( rh-anti-CD20zumab) in biological matrix. The specificity, precision and accuracy of each method at correspondingly different linear range showed good results. For ELISA, the precisions of intra-day and inter-day were both <19 . 5%, the relative error was from-18 . 2% to 17 . 6%;For FCA, the precisions of intra-day and inter-day were both <19. 0%, the relative error was from -18. 9% to 18. 4%. The sensitivity of ELISA was significantly higher than that of FCA. The quantitative ranges of ELISA and FCA methods were 0. 04-5. 0 mg/L and 3. 1-200 mg/L, respectively. The concentrations in serum samples and pharmacokinetics analysis were determined by both of two methods after vein drip administration of rh-anti-CD20zumab in rhesus monkeys. Pharmacokinetics data showed that there was excellent consistency between results obtained by two methods at the given dose. We believe that the novel FCA with high speed and high sensitivity can be used to perform PK and PD study of cell surface antigen-targeted antibody derivatives.
6.Clinical characteristics and management of recurrent retinal detachment in silicone oil tamponade eyes
Qing BAO ; Jia CHEN ; Yiqiao XING ; Lei DU ; Haifeng MEI
Recent Advances in Ophthalmology 2017;37(6):576-578
Objective To explore the characteristics and surgical managements of recurrent retinal detachment in silicone oil tamponade eyes.Methods The records of consecutive series of 134 patients (134 eyes) with recurrent retinal detachment in silicone oil tamponade eyes from January 2012 to December 2015 in our hospital were reviewed retrospectively,the vitrectomy combined with silicone oil replacement or scleral buckling procedure were performed.The follow-up time was 6 months,the surgical efficient was evaluated.Results 101 eyes underwent vitrectomy combined with silicone oil replacement.Retina was completely reattached in 79 eyes,and vitrectomy was reperformed in the left 22 eyes,the successful rate was 78.2% (79/101);The visual acuity improved in 16 eyes,unchanged in 56 eyes,decreased in 29 eyes;The intraocular pressure of 31 eyes increased more than 25 mmHg (1 kPa =7.5 mmHg).33 eyes underwent scleral buckling procedure.Retina was completely reattached in 23 eyes,and vitrectomy combined with silicone oil replacement was performed in the left l0 eyes,the successful rate was 69.7% (23/33);The visual acuity improved in 5 eyes,unchanged in 16 eyes,decreased in 12 eyes;The intraocular pressure of 14 eyes increased more than 25 mmHg.Conelusion For limited retinal detachment caused by inferior or peripheral holes,proliferative vitreoretinopathy in the A or B-class,the refractive medium does not affect the fundus examination,scleral buckling surgery is preferred;For the hole in the posterior pole or extensive retinal detachment caused by giant retinal holes,proliferation or retinal fixed fold formation,vitrectomy combined with silicone oil replacement is a better option.
7.The Effect of Acromioplasty in the Suture Bridge Rotator Cuff Repair under Arthroscopy
Cong XU ; Jiangtao REN ; Jia LI ; Haifeng DAI ; Fei XU ; Yuanliang DU ; Zhihuai LI ; Yongming LV
Chinese Journal of Sports Medicine 2017;36(6):526-530
Objective To explore the clinical results of the arthroscopic acromioplasty in the rotator cuff repair.Methods Sixty-five patients (42 males and 23 females) to receive suture bridge repair under the arthroscopy between May 2012 and May 2014 were selected and randomly divided into an experimental group and a control group.The experimental group was given suture bridge repair with acromioplasty,while the control group underwent the suture bridge repair without acromioplasty.The time of operation was recorded.The range of motion (ROM) of the shoulder,the UCLA shoulder scoring system (ULCA) score,American orthopedic surgeon scoring system (ASES) score,the visual analogue scale (VAS) score and Constant score were observed 3 and 12 months after the operation.Results There were no significant differences in age,sex and the affected side between the two groups.Significant differences were found between the two groups in the duration of the operation (t=-18.5,P<0.05).There were no significant differences in the ROM and the ULCA,ASES,VAS and constant scores of the shoulder 3 and 12 months after the operation.Conclusion No significant differences were found in the ROM and the shoulder function scoring between giving acromioplasty under the arthroscopy or not.However,the operation time of undergoing acromioplasty under the arthroscopy was longer.
8.Energy Spectrum Scanning of Thyroid Nodules:A Study Based on Support Vector Machine
Chuangbo YANG ; Siqiang NIU ; Yongjun JIA ; Yong YU ; Haifeng DUAN ; Taiping HE
Chinese Journal of Medical Imaging 2015;(3):231-234,240
PurposeSupport vector machine (SVM) is a machine learning method based on statistical learning theory of Vapnik-Chervonenkis (VC) dimension structure and risk minimization theory. We analyzed the gem spectrum CT scan data of patients with thyroid nodules and established the SVM diagnostic model. The experimental targets were then reduced and the forecast analysis was carried out based on SVM model. The diagnostic model and experimental methods were proved to provide guidance for clinical diagnosis of thyroid nodules.
9.Prevalence rate of healthcare-associated infection in patients in a tertiary first class military hospital
Haifeng LI ; Yandong ZHANG ; Lina YU ; Dongchun ZHENG ; Yue ZUO ; Liping DUAN ; Chen JIA ; Jinke SUN
Chinese Journal of Infection Control 2016;15(10):769-772
Objective To investigate the current situation and related risk factors of healthcare-associated infection (HAI),so as to provide evidence for making prevention and control measures of HAI.Methods On November 26, 2014,a combination method of bedside visiting and medical record reviewing was adopted to survey HAI status, pathogen examination,and antimicrobial application in all hospitalized patients in a tertiary first class military hospi-tal.Results A total of 1 657 hospitalized patients were investigated,66 patients developed 71 times of HAI,HAI rate and HAI case rate were 3.98% and 4.28% respectively.The top 4 departments with HAI prevalence rates were departments of neurosurgery (24.49%),hematology(19.05%),cadre ward(13.73%),and burn surgery (10.91 %).The top 5 HAI sites were lower respiratory tract (40.85%),urinary tract(23.94%),upper respirato-ry tract(12.68%),surgical site(9.86%),and gastrointestinal tract(5.63%).Of 66 cases of HAI,39 (59.09%) patients sent specimens for culture,a total of 48 pathogens were cultured,the major isolated bacteria was Esche-richia coli (n = 10,20.84%),followed by Staphylococcus aureus ,Klebsiella pneumoniae ,and Pseudomonas aeruginosa ,each was 7 (14.58 %)respectively.The usage rate of antimicrobial agents was 34.40%,specimen de-tection rate in patients receiving therapeutic and therapeutic+prophylactic antimicrobial agents was 59.28%.Risk factors for HAI were age <2 years old or >60 years,with respirator,tracheotomy,urinary tract catheterization, arteriovenous catheterization,hemodialysis,and surgery,difference was significant(all P <0.05).Conclusion Mo-nitoring on key departments and key sites of HAI should be strengthened,antimicrobial agents should be used rationally based on pathogenic detection results,specimen pathogenic detection rate should be improved,and effective prevention and control measures needs to be taken according to the risk factors of HAI.
10.Animal biodistribution and pharmacokinetics study of ~(131)I-labelled rch24
Haiwei JIA ; Qing NIE ; Haifeng SONG ; Baozhen ZHU ; Xiao SUN ; Xiaojun MIAO ; Lun OU
Cancer Research and Clinic 2009;21(11):724-727
Objective To evaluate biodistribution and pharmacokinetics pattern of ~(131)I-labeled rch24which is the region-grafted (humanized) anti-carcinoembryonic antigen (CEA) monoclonal antibody in nude mice. Methods Nude mice bearing cancer xenografts received intravenous injections of ~(131)I- rch24, then blood, plasma, heart, liver, spleen, lung, kidney, tumor and other tissues were taken at different time point for determination the concentration of radioactivity and calculate the T/NT value. Nude mice were packeted randomly to four group of high, medium, low dose and continuous administration, blood drug concentration was detected by ELISA method at the different intervals. Then, draw the concentration-time curve and calculate the pharmacokinetics paramete. Results After administration, radioactivity of the tumour was significantly enhanced whereas radioactivity of normal tissues decreased gradually. For single administration, at the dose of low to medium, pharmacokinetics pattern was linearity -kinetics whereas for high dose group,pharmacokinetics paramete shown some behavior of non-linearity-kinetics. Conclusion Our results suggest that the ~(131)I-labeled region-grafted (humanized) anti-CEA monoclonal antibody rch24 exhibit a considerable targeting activity so as to ~(131)I radioisotopes can be concentrated specifically in tumor. The pharmacokinetics pattern of this medicine was different at different dose.