Objective To investigate the value of combined detection of serum alpha-fetoprotein (AFP)and Golgi protein-73 (GP73)in the diagnosis of hepatocellular carcinoma (HCC)and to provide a basis for early diagnosis and differential diagnosis of HCC.Methods A total of 408 patients hospitalized in Baoji Central Hospital from June 2012 to May 2013,as well as healthy persons who had normal test re-sults in physical examination,were included in the study,and their specimens were collected.These patients were classified into HCC group (n=142),chronic hepatitis group (n=156),and liver cirrhosis group (n=110).Serum levels of AFP and GP73 in the three groups were measured by electrochemiluminescence immunoassay and double -antibody sandwich enzyme -linked immunosorbent assay,respectively. Comparison of test results between groups was made by analysis of variance,and comparison of rates was made by chi-square test.The sen-sitivity and specificity of the two indicators for the diagnosis of HCC were calculated using MedCalc statistical software.Results The HCC group had significantly higher serum AFP and GP73 levels than the liver cirrhosis group and chronic hepatitis group (P<0.05);the liver cirrhosis group had significantly higher serum AFP and GP73 levels than the chronic hepatitis group (P<0.05).The sensitivity and speci-ficity of the two indicators for the diagnosis of HCC were 95.8% and 98.6%,respectively,showing significant differences compared with those of each indicator alone (P<0.05).Conclusion Combined detection of serum AFP and GP73 has high diagnostic value and clinical significance for HCC,and they can be used as indicators for early diagnosis and differential diagnosis of HCC.

Objective Dynamic observation of plasma endotoxin (ET),plasma TXA2 and PGI2 levels and clinical symptoms in patients with severe acute pancreatitis (SAP),the protective effect of Salvia miltiorrhiza ligustrazine on the intestinal barrier in severe acute pancreatitis was studied.Methods The 60 cases of severe acute pancreatitis treated in our hospital from August,2014 to October 2015 were studied as the research object,and were randomly divided into the control group and the danchuan group.Two groups of patients received conventional treatment,such as diet,gastrointestinal decompression,early active fluid resuscitation,maintenance of stable electrolyte,anti infection,inhibition of pancreatic secretion,parenteral nutrition and other support treatment,Dan Chuan group on the basis of conventional treatment combined with 10 ml of compound Salvia miltiorrhiza ligustrazine injection of 5% glucose injection,250 ml intrave-nous drip,1 times a day.Two groups of patients with 14 days for a period of treatment,the clinical symp-toms of the two groups were observed after the end of treatment,the clinical symptoms and the changes of la-boratory index.Results ⑴In the group of Dan chuan,the time of patients relieve intestinal pneumatosis or intestinal obstruction,abdominal pain were less than the control group,the difference was statistically sig-nificant (P <0.05).⑵ The recovery time of plasma endotoxin,serum amylase,and leukocyte in the Dan Chuan group were shorter than those in the control group (P <0.05).⑶The incidence,mortality and inci-dence of complications in the control group were significantly lower than those in the control group,but the difference was not statistically significant (P >0.05).Conclusions Salvia miltiorrhiza ligustrazine has a significant effect on severe acute pancreatitis.It can improve the intestinal microcirculation by correcting the balance between PGI2 and TXA2 ,and can promote the recovery of intestinal function,reduce the incidence of intestinal bacterial and endotoxin,and reduce the incidence of complications.

Objective To investigate the biology reference intervals for five parameters of thyroid gland function(TSH,T3, T4,FT3 and FT4)in Baoji area,in order to provide evidence for effective filtration and diagnosis.Methods Refer to the files recommended by (NCCLS)C28-A2 about the description of definition,establishment and confirmation,five parameters of thyroid gland function were measured in 4 820 healthy persons by using Roche E601 electrochemical immunoassay analyzer and its assemble reagent,calibration materials and quality control,the statistical method was applied to the data processing by different gender,to establish the biology reference intervals of each parameters.And compared with National Guide to Clinical Laboratory Procedures(Third Edition).Results The range of 95% normal reference intervals of TSH,T3,T4,FT3 and FT4 in male were 0.20~4.28 mIU/L,1.22~2.46 nmol/L,64.64~149.00 nmol/L,3.00~6.04 pmol/L,11.93~22.21 pmol/L respectively,and in female were 0.20~4.52 mIU/L,1.24~2.44 nmol/L,67.60~142.40 nmol/L,2.96~5.76 pmol/L and 11.86~21.54 pmol/L,respectively.Compared the level of TSH,FT3,FT4 between male and female, differences were statistical significance(t=3.962,7.570,5.132;P=0.042,0.026,0.033).Compared the level of T3,T4 be-tween male and female,there was no statistical significance (t=0.000,1.151;P=1.000,0.120).The study compared with National Guide to Clinical Laboratory Procedures (Third Edition),the results of T3,T4,FT3 and FT4 in male were statisti-cal significance (t=9.755,4.349,114.7,79.82;P=0.000,0.003,0.000,0.000),the results of TSH,T3,FT3 and FT4 in female were statistical significance (t=5.304,9.548,128.9,72.99;P=0.002,0.000,0.000,0.000).Conclusion The in-vestigation of biology reference intervals for five parameters of thyroid gland function in Baoji area can provide more precise-ly reference data for clinical diagnosis and treatment.

Objective To investigate the clinical application of serum retinal binding protein and cystain C determination in hem-orrhagic fever with renal syndrome(HFRS).Methods The serum concentrations of RBP,CysC,Urea and Cr were detected for 124 patients with HFRS(patients group)and 100 healthy people who underwent physical examination during the same period(control group),the date were analysed by SPSS19.0 software.Results The concentrations of RBP,CysC,Urea and Cr increased signifi-cantly in febrile stage of HFRS,arrived at peak in oliguria stage,and then declined gradually,there were statistically significant differences between each stage and negative control stage(P <0.05).RBP and CysC had a good positive correlation with Urea and Cr,the correlation coefficient between RBP and Urea or Cr were 0.826 and 0.892,respectively(P <0.05)while with CysC were 0. 841 and 0.924,respectively(P <0.05).The positive rates of RBP,CysC,Urea,Cr in febrile stage were 85.48%,95.16%,69.35%, 83.06% respectively,while in convalescent stage were 67.74%,74.19%,46.77% and 58.06% respectively.Conclusion RBP and CysC are good indicators for diagnosing HFRS,which also have good correlation with Urea and Cr,which are recommended in clini-cal application.

Objective To study and discuss the clinical curative effect of dampness Kushen Decoction in the treatment of psoriasis damp heat syndrome.Methods100 cases of psoriasis vulgaris(damp heat syndrome) patients who treated in our hospital from January 2014 to October 2016 were selected as the research object, the patients were divided into two groups by taking the single blind randomly grouping method, each group had 50 cases, the control group used Tacrolimus Ointment chemophlebitis, the observation group treated with dampness Kushen decoction on the basis of the control group, the total effective rate and skin injury score were compared between two groups.ResultsAfter 8 weeks of treatment, the total effective rate in the observation group was significantly higher than the control group (P<0.05);after treatment, the PASI scores in two groups were significantly decreased (P<0.05), but the PASI score in the observation group was significantly lower than the control group (P<0.05).ConclusionThe dampness Kushen Decoction in the treatment of psoriasis damp heat syndrome has significant curative effect, can effectively promote the skin damage and improve the prognosis better.

Objective To investigate the expression level of hyper sensitivity C-reactive protein (hsCRP) in children infected with different pathogens in order to enhance the application value of hsCRP .Methods One hundred and one children patients with infection from March 2010 to March 2015 were selected as the research subjects and divided into the bacterial infection group (67 ca-ses) and mycoplasma infection group (34 cases) according to the hematology and bacterial culture results .Contemporaneous 50 healthy children were selected as the control group .Venous blood was collected for detecting hsCRP ,white blood cell(WBC) and neutrophil percentage(NEUT% ) .Results The levels and abnormal rates of hsCRP and WBC in the bacterial infection group were significantly higher than the those in the mycoplasma infection group and control group (P<0 .05);NEUT% and abnormal rate in the mycoplasma infection group was higher than that in the other two groups (P<0 .05) .The levels and abnormal rates of hsCRP and WBC after treatment in the bacterial infection group were lower than those before treatment ,the difference was statistically sig-nificant(P<0 .05);the area under the ROC curve of hsCRP for diagnosing bacterial infection wa maximal ,which was 0 .68 ,the sensitivity and specificity were 89 .9% and 88 .3% respectively .Conclusion The hsCRP level detection has early diagnostic value for child bacterial infection and can dynamically reflects the curative effect .

Purpose To investigate the significance of PET/CT in differential diagnosis of incidental thyroid benign or malignant lesions with high fluorodeoxyglucose (FDG) uptake.Materials and Methods Forty cases with focal high FDG uptake lesion in thyroid accidently detected by PET/CT were retrospectively collected. The subjects were dived into physiological uptake group (n=18), benign lesion group (n=16) and malignant lesion group (n=6) according to the pathology or follow-up examinations. The average SUVmax of different groups was analyzed, and ROC curve was used todetect benign or malignant lesions.Results The average SUVmax was 3.7±1.1 in physiological uptake group, 4.9±2.3 in benign lesion group and 9.5±4.8 in malignant lesion group. Significant difference was noticed among the three groups. The average SUVmax in malignant lesion group was higher than that of the other two groups with statistical difference (P<005). No significant difference was observed between physiological uptake group and benign lesion group (P>0.05). ROC analysis showed that the sensitivity and specificity for thyroid malignant tumor were 100.0% and 87.2% at SUVmax of 8.5.Conclusion18F-FDG PET/CT plays an important role in differentiating benign lesions of thyroid from malignance, but it is not helpful to identify benign lesions from physiologic uptake.

Background:Secondary lymphoid tissue chemokine( SLC)is involved in lymphoid homing and anti-tumor immune response,and has a chemotactic effect on intestinal lymphocytes. Several animal studies have shown that SLC is involved in the occurrence and development of ulcerative colitis( UC). Aims:To investigate the expression and significance of SLC in UC. Methods:Forty active UC patients from Dec. 2010 to Dec. 2012 at Affiliated Hospital of Nantong University were enrolled,and 20 healthy volunteers were served as controls. Expression of SLC in the colon mucosa was detected by immunohistochemistry,and its relationship with severity of UC was analyzed. Results:SLC was positively expressed in all UC patients,while was negatively or weakly positively expressed in controls. Expression of SLC in UC patients was significantly higher than that in controls(4. 16 ± 0. 78 vs. 0. 52 ± 0. 11,P<0. 05). Expression of SLC was correlated with the severity of involvement of UC. Conclusions:Expression of SLC participates in development and progress of UC. SLC may play an important role in the induction of local damage and pathological changes of UC.

Objective To summarize the construction,upgrading process and clinical application of prostate cancer database (PC-Follow).Methods From September 2008 to December 2014,we designed two versions of off-line PC-Follow clinical databases based on Microsoft Access to support clinical follow-up and research in prostate cancer patients.Then,in order to achieve unified management of clinical data import and multi-center clinical research,we further upgraded PC-Follow to a browser/server (B/S) schema based web database.After upgrade,a simulated multi-center operation pattern was adopted to transfer all existed data-We then attempted to linkage our database with the hospital information system (HIS) and had eventually undertaken reality and stress testing on 3,124 cases'data migration in the hospital.The nationwide software testing and personnel training was conducted in 12 major medical centers.Results Twice of upgrade and alteration of schema of PC-Follow preserved the original user interface.Multiple functions,including data import,follow-up,research support,were obviously enhanced.Notably,the perfection in multi-user and hierarchical access control meet the future needs for multi-center clinical research.Via the import tool for PC-follow V3.0,we successfully migrated all data from the original one.After linking our database with the HIS software by one-way transmission protocol,the safety data extracting could be achieved.Specified administrators were appointed to process and analyze PC-Follow data in bulk,and data were voluntarily shared between attending centers,and showed stable performance on the 3,124 patients in our database.Feedback from nationwide multicenter training and testing indicated that our database was easy,readily accessible,with functionally stable modules,accurate website displays,and ideal response time.Conclusions The online PC-Follow database with hierarchical access provides support for multi-center clinical research in prostate cancer.The off-line PC-Follow database also grants certain degrees of data management for underdeveloped clinical centers,and it has its advantage to serve as prototypes for data migration to future online PC-Follow database once conditions are ripe.

Objective To summarize the clinical medicine treatment in elderly patients with renal dysfunction,in order to discover the existing problems and analyze the reasons.Methods One hundred and eighty cases of senile patients with renal insufficiency were randomly selected in the second half of 2012 to analyze medication states based on the medication standard.Results Medical advices prescribed by doctors showed that 7.5 ％ of drugs were forbidden used in patients with severe renal insufficiency,and 34.1％ of drugs should be used with caution or reduction requirements.Each department and system had irrational drug using.Conclusions There are a large problem in the clinical medication in patients with renal insufficiency,and a reasonable medication standard needs to be established to find out potential irrational drug using so as to reduce the possibility of adverse reactions.