Midkine (MK),a member of the heparin-binding growth factors,was firstly discovered in mouse embryonic carcinoma cells by Japanese scholars.MK functions in a variety of biological processes,including neurogenesis,activation of plasminogen activator,wound tissue repair and inflammatory response.More importantly,MK is closely related to tumor progress and metastasis.Current studies show that MK is over-expressed in various human malignant tumors,making it as a promising tumor biomarker and possibly a new molecular target for tumor therapy.This review will illustrate the molecular structure and functional mechanisms of MK,as well as its roles in the diagnosis,progression monitoring and prognosis prediction of colorectal cancer.

Objective:To explore the role of the inpatient education platform of internet plus 317 nursing that was assistant of health education of nurse in the clinical nursing work.Method: 100 patients with orthopedic disease were divided into observation group (50 cases)and control group (50 cases) as random number table. The patients of observation group received admission education and health education by using internet plus 317 nursing, which was assistant of health education of nurse, while the patients of control group received routine education mode including dictate of cramming mode, blackboard newspaper, propaganda handbook and telephone follow-up and so on. And then the score of anxiety self-assessment scale (SAS) and nursing satisfaction degree between the two groups were compared and analyzed.Result: The scores of SAS of observation group in admission and 7 d later were (49.66±9.126) points and (46.32±7.02) points, respectively, and the scores of them of control group respectively were (48.18±9.260) points and (45.22±6.53) points. And the differences between the two time point in each group were not statistically significant (t=0.805.81,t=0.812,P>0.05). At discharge, the score of observation group (39.08±3.59) points was significantly lower than that of control group (42.86±4.25) points (t=4.81,P<0.05). Besides, the satisfaction degree of observation group was significantly better than that of control group(x2=0.925, P=0.031).Conclusion: The inpatient education platform of internet plus 317 nursing can guide health of patients in time, and quickly solve problems during hospitalization, and reduce the SAS score of patients, and increase the patient's satisfaction degree for clinical nursing quality.

Objective　To study the malignant or benign characters of lung mini nodules for diagnosis and treatment.Methods　A retrospective analysis was performed on 80 post-operative cases with pathologically confirmed malignant or benign lung mini-nodules.Results　Lung mini-nodule resection cases accounted for 17.7%(80) out of a 451-cases group.Malignant cases accounted for 73.7%(59/80)，benign cases 26.3%(21/80).Most of the malignant cases were squamous cell carcinoma and adenocarcinoma,while most of the benign cases were tuberculosis and inflammatory pseudo-neoplasms.As for pTNM staging in malignant cases,there were 28 in pT1N0M0，19 in pT1N1M0,1 in pT1N1M1,10in pT1N2M0 and 1 in pT1N2M1;the average survival time was 29.4 months.The accurate rate of pre-operative X-ray and CT scan was 45% and 76.2% respectively.Conclusion　For patients with lung mini-nodules ＜2 cm CT value at 37HU,and the nature unknown ,resection is suggested.

ObjectiveTo study the changes and clinical significance of DNA ploidy,S-phase fraction(SPF),proliferating index(PI) in colorectal carcinoma.MethodsIn 40 cases of colorectal carcinoma (colorectal carcinoma group ),40 cases corresponding cancer-adjacent tissue (cancer-adjacent tissue group ),12 cases of precancerous lesions (precancerous lesions group) and 10 cases of normal colorectal mucosa coli (normal colorectal mucosa coli group),DNA ploidy,SPF and PI were detected with flow cytometry and compared.ResultsDNA diploid was 7 cases,DNA heteroploid was 33 cases in colorectal carcinoma group,35,5 cases in cancer-adjacent tissue group,10,2 cases in precancerous lesions group,10,0 case innormal colorectal mucosa coli group,there were significant differences among them(P＜ 0.01 ).SPF and PI in colorectal carcinoma group (35.36 ± 7.45,42.92 ± 6.81 ) were significantly higher than those in cancer-adjacent tissue group (20.82 ±5.51,31.34 ±4.88),precancerous lesions group (21.13 ± 5.07,31.70 ±5.59) and normal colorectal mucosa coli group ( 19.93 ± 3.73,32.01 ± 4.99),there were significant differences among them(P＜ 0.01 ).DNA ploidy was significantly correlated with Dukes staging (P=0.027) and the differentiation of colorectal carcinoma (P =0.030).ConclusionsDNA ploidy,SPF,PI may get some molecular biologycharacteristic and proliferation activity of colorectal carcinoma at molecule level,which may be helpful for treatment and evaluation of prognosis of colorectal carcinoma.

[Objective]To observe clinical value of multi-slice spiral X-ray computed tomography(MSCT)volume rendering technique(VRT)and multi-planar reconstruction(MPR)in classification and treatment of tibial plateau fracture(TPF).[Method]From August 2005 to February 2009,49 fratures in 45 patients with TPF were treated.Their clinical data were studied retrospectively.There were 32 males and 13 females.The patients were aged from 20-65 years(mean 46 years).Four patients suffered bilateral TPF.All patients were examined with standard X-rays and 64-slice spiral CT scanner.Axial CT scans were processed with VRT and MPR.Their X-ray plain films and 64-slice spiral CT seans(including VRT and MPR)were performed to determine their fracture types and protocol.[Result]According to the VRT and MPR findings,13 fractures(13/49)classified by X-ray plain film were corrected,protocols were changed for 9 fractures(9/49),and 15 fractures(46.9%)of intereondylar eminence of the tibia and 6 fractures(28.6%)of proximal fibula were underdiagnozed.[Conclusion]MSCT(VRT+MPR)can clearly show the area and degree of tibial plateau collapse,the details of TPF and the delitescence fracture,help to determine TPF types,decrease the underdiagnosis rate and choose a rational protocol.The use of combined VRT and MPR is a supplementary means for X-ray plain film and axial CT scanning.which has great clinical value.

BACKGROUND:Reduction and fixation are presently used for middle and inferior humeral fractures. The methods of fixation approach include anterolateral (including modified upper arm anterolateral), posterior and medial approaches. The repair approach of middle and inferior humeral fractures remains controversial. OBJECTIVE:To compare efficiency and safety of anterolateral and medial approach fixation with compression plate and screw for middle and inferior humeral fractures. METHODS:A total of 90 cases of middle and inferior humeral fractures treated in Chongqing Red Cross Hospital were equaly and randomly divided into experimental group and control group. In the control group, patients were subjected to compression plate and screw through anterolateral approach. In the experimental group, patients were subjected to compression plate and screw through medial approach. Operation time, intraoperative blood loss, complications and the recovery of shoulder joint and elbow joint function were compared in patients of both groups. RESULTS AND CONCLUSION:Intraoperative blood loss was significantly lower in the experimental group than in the control group (P < 0.05). No significant difference in operation time and fracture healing time was found between the two groups (P > 0.05). During 6 months of folow-up, no significant difference in shoulder joint function Neer score and elbow joint function Mayo score was detected between the two groups (P > 0.05). During the folow-up period, no wound infection, bone nonunion or chronic osteomyelitis appeared in both groups. These data confirm that the effects of medial approach and anterolateral approach for middle and inferior humeral fractures are similar. Medial approach leads to less blood loss, can effectively make up for the defects of the traditional repair scheme, and can be considered as one of fixation approach for middle and inferior humeral fractures.

Objective To analyze the clinical efficacy of improved incision septoplasty in treatment of allergic rhinitis and its effect on the life quality of patients.Methods A total of 42 patients with persistent allergic rhinitis and merge nasal septum in our hospital from July 2013 to April 2015 were divided into observation group and control group.The observation group were treated by improved incision septoplas-ty,and the control group received traditional KillIan incision treatment.The clinical efficacy,survival quality and visual analog scale between two groups were compared.Results After treatment,The total efficiency rate of observation group(94.24%)was significantly higher than that of control group(85.71%)(χ2 =7.15,P <0.05).The nasal congestion,nasal itching,sneezing and other symptoms score of observa-tion group were significantly lower than those of control group(P <0.05).The score of nasal problems,eye problems,eye problems and non-nasal sleep disorders in observation group were also significantly lower than those in control group(P <0.05).Intraoperative situation of ob-servation group was significantly better than that of control group(P <0.05).Conclusion Improved incision septoplasty has a good clinical efficacy for patients with persistent allergic rhinitis and merge nasal septum,which can significantly improve the clinical symptoms and life quality of patients.

This paper is to establish a mathematical mapping m odel of different medical images for the same human tissue or organ.According to the mathematical mapping model of gray level,the merging and matching methods of medical image information,including CT and MRI,are studied.The coordinate sy stems of different medical images are normalized to be at the same three-dime nsional position,and then the new-mode images are showed to the users.This study establishes the basis of three-dimensional visibility of the information merge of medical images with different mode.

[Objective]To explore the surgical technique and clinical results of minimally invasive incision dynamic hip screws (DHS) in treatment of intertrochanteric fractures in the elderly.[Method]From August 2001 to January 2006,37 old cases (15 males and 22 females, aged from 72 years to 92 years, mean 81.5 years) with intertrochanteric fractures were treated with closed reduction and DHS using minimally invasive technique in our hospital. The procedure included following and critical steps:1～2 k-wires was inserted into the upper part of the femoral head through great trochanter percutaneously after operation. A guide wire was inserted into the center of the femoral head according to 135? collodiaphyseal angle; then, a small incision (4～5.5 cm) was made and along the guide wire for inserting a screw into the femoral head. The guide wire was removed and the side plate was slipped under the soft tissue into to tightly contact the lateral surface of femur, and followed by the side plate barrel being seated on the screw.[Result]The operation time was 40～75 minutes (average 60 minutes) The mean amount of blood loss in, operation was 55 ml. The mean amount of hemoaleh:in showed no significant difference between postoperation and preoperation. The following-up period averaged 15 months (11～18 months) The clinical hone healing lasted for 10～15 weeks. Two cases had mild coxa vara, there were no incision infection, fixation failure or lower extremity rotatory deformity. The excellent rate of joint function was 94.6% according to DONG's assay standard. [Conclusion]Minimally invasive incision DHS has the advantages of shorter operation time, less blood loss, little trauma, less complications and quick functional recovery and is an effective and safe method for treatment of femoral intertrochanteric fractures in the elderly.

[Objective]To evaluate the clinical characteristics and effect of operative treatment for the ossification of ligamentum flavum thoracic spinal stenosis (OLF-TSS). [Methods]From October 1998 to February 2007,38 patients with OLF-TSS were treated with en bloc hemi-articular process laminectomy. In this group,25 cases were male,and 13 cases were female. The average age was 48 years (range,29～71 years). Totally 81.6% of the lesion was between T10～L1 intervertebral disc space.The average history was 10 months. There were 5.3 percent with protrusion of intervertebral disc of thoracio spine.[Results]Thirty-eight cases were followed-up for 1～8 years,with an average of 3 years and 6 months. Dural injury occurred in 4 cases of 38,3 cases appeared postoperative cerebrospinal fluid leakage. All cases were cured conservatively mainly with bed rest in the prostrate position. According to WANG's evaluation,25 had excellent results,11 had good results,2 had no change and none was poor,The excellent to good rate was 94.7% in this group. There were no patients with the wrong level. No patient neurological functions were deteriorated.[Conclusion]The chinical features of OLF-TSS are very complex. The common symptoms of the disease are progressive onset of numbness,weakness,low back pain,intermittent claudication of the lower extremities,constriction on trunk or lower limbs and sphincter dysfunction. The clinical presentation and neurological examination associated with X-ray film,MRI and CT were the important means of the diagnosis of the disease. Removal of the posterior wall of the thoracic spinal canal via the technique of en bloc hemi-articular process laminectomy is safe and effective to treat OLF-TSS. The strategy for diminishing fault and complications is to discriminate pathogenic segment (locating-diagnosis) and follow the principles of surgical procedure.