Objective: To study the pharmacology and toxicology of the extracts from Arcangelisia gusanlung (EAG). Methods: The anti-inflammatory activities were investigated using various inflammatory models including ear edema induced by xylene in mice, paw edema induced by carrageenan, and cotton pellet granuloma in rats. The analgesic effect was observed in hot-plate test and writhing test in mice and the antipyretic effect was observed in rat fever model induced by yeast. The antitussive action was tested in mice by sequential method and expectorant action was evaluated by tracheal excretion of phenol red. The antidiarrhea function was observed on normal intestinal propulsion of mouse model of diarrhea induced by decoction of Sennae Folium. The toxicity was measured by toxicological experiment. Results: Each dose of EAG could significantly inhibit the paw edema, cotton pellet granuloma, and intestinal propulsion. EAG significantly reduced writhing times and amount of wet manure. Obvious antipyretic action to fevered rat was observed. EAG obviously increased the tracheal excretion of phenol red and prolonged the latency of cough. No toxic reaction was shown in the observed period, and the maximum tolerance dose of mice was equivalent to 1360 times of common-used dose in human. Conclusion: The clinical dosage of EAG is safe, and its anti-inflammatory, analgesia, antipyresis, antitussive, expectorant, and antidiarrhea effects are significant. © 2013 Tianjin Press of Chinese Herbal Medicines.

Objective To investigate the effect of radiotherapy combined with interventional therapy on the changes of vascular endothelial growth factor (VEGF) in patients with advanced cervical cancer.Methods 62 cases of cervical cancer collected in the first people’s hospital of jingzhou city during January 2009 to February 2014 were randomly divided into two groups.The patients in group A were treated with radiotherapy, and the patients in group B were treated with radiotherapy combined with interventional therapy.The level of vascular endothelial growth factor in two groups after treatment was measured by enzyme-linked immunoassay.Results The level of vascular endothelial growth factor of group A was ( 241.77 ±85.86 )μg/L, significantly higher than ( 124.66 ±65.86 )μg/L of group B.The level of vascular endothelial growth factor was related with cervical deterioration degree, tumor stage and lymph nodes metastasis (P<0.05),not with the patient’s age, and the size of the tumors.The level of vascular endothelial growth can reflect the clinical efficacy of patients after therapy (P<0.05).Conclusion Vascular endothelial growth factor level has a close relationship with the degree of deterioration of cervical cancer, tumor staging, lymph node metastasis and the curative effect after the interventional therapy.

OBJECTIVETo study the effect of dragon's blood powder with different grain size on the transdermal permeability and adhesion of ZJHX paste.

METHODDragon's blood powder with grain sizes of 4, 19, 55 microm were got by ultrafine grinding technology, and then prepared into rubber pastes A, B, C and D, together with dragon's blood powder with grain size of 93 microm of original description. Franz diffusion cell method was adopted to compare the difference in transdermal permeability of dragon's blood powder with different grain sizes, with dracorhodin as the index, and compared their effect on the adhesion of pastes with initial adhesion, permanent adhesion and peel strength as the indexes.

RESULTQ(s)-t equations of pastes A, B, C, D were as follows: Q(s)=1.369 6t + 3.985 5, Q(s) = 1.262 8t +3.738 1, Q(s) = 1.192 3t + 3.320 6, Q(s) = 1.152 2t + 2.366 1, respectively, which showed that the adhesion of A was the best good.

CONCLUSIONWith the decrease in the grain size of dragon's blood powder, accumulative penetration of dracorhodin increases, which facilitates transdermal permeability and adhesion.

Absorption ; Animals ; Croton ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacokinetics ; Mice ; Particle Size ; Plant Extracts ; chemistry ; pharmacokinetics ; Resins, Plant ; chemistry ; pharmacokinetics ; Skin ; metabolism ; Skin Absorption

Adult ; Ascorbic Acid ; administration & dosage ; analysis ; Humans ; Male ; Nutrition Surveys ; Occupational Health ; Riboflavin ; administration & dosage ; analysis ; Thiamine ; administration & dosage ; analysis ; Vitamins ; administration & dosage ; analysis

An analysis method has been established to test 27 elements (Li, Be, B, Mg, Al, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, As, Sr, Mo, Cd, Sn, Sb, Ba, La, Hg, Pb, Bi) in Maca nationality's medicine with microwave digestion-ICP-MS. Sample solutions were analyzed by ICP-MS after microwave digestion, and the contents of elements were calculated according to their calibration curves, and internal standard method was adopted to reduce matrix effect and other interference effects. The experimental results showed that the linear relations of all the elements were very good; the correlation coefficient (r) was 0.9994-1.0000 (Hg was 0.9982) ; the limits of detection were 0.003-2.662 microg x L(-1); the relative standard deviations for all elements of reproducibility were lower than 5% (except the individual elements); the recovery rate were 78.5%-123.7% with RSD lower than 5% ( except the individual elements). The analytical results of standard material showed acceptable agreement with the certified values. This method was applicable to determinate the contents of multi-elements in Maca which had a high sensitivity, good specificity and good repeatability, and provide basis for the quality control of Maca.
Lepidium ; chemistry ; Mass Spectrometry ; methods ; Microwaves ; Reproducibility of Results ; Trace Elements ; chemistry ; isolation & purification

To study the application characteristics of copovidone (PVP-S630) in Xinyueshu extracts during the spray drying process, and its effect on such pharmaceutical properties as micromeritics and drug release behavior. PVP-S630 was added into Xinyueshu extracts to study on the spray drying, the effect of different dosages of PVP-S630 against the wall sticking effect of the spray drying, as well as the power property of Xinyueshu spray drying power and the dissolution in vitro behavior of the effective component of hyperoside. The results showed that PVP-S630 revealed a significant anti-wall sticking effect, with no notable change in the grain size of the spray drying power, increase in the fluidity, improvement in the moisture absorption and remarkable rise in the dissolution in vitro behavior of hyperoside. It was worth further studying the application of PVP-S630 in spray drying power of traditional Chinese medicine.
Absorption ; Desiccation ; methods ; Drug Compounding ; methods ; Drugs, Chinese Herbal ; chemistry ; Porosity ; Powders ; Pyrrolidines ; chemistry ; Vinyl Compounds ; chemistry ; Wettability

Fifty-six patients with Graves' ophthalmopathy(GO)were treated with antithyroid drug and oral prednisone for three months,TSH receptor antibody(TRAb)level was reduced,GO activity and severity of some patients were ameliorated but still positively associated with TRAb.It suggests that TRAb not only triggers off GO but also plays a possible role in the maintenance of the autoimmune process in GO.

OBJECTIVETo study the changes of soil fertility in Sheyang county where Chrysanthemum morifolium has been cultivated for more than 30 years and to develop the special fertilizer for cultivation of C. morifolium.

METHODThe pH values, organic matter and the contents of total and available N, P, K and Zn in the soil layer of 0 to 40 cm, as well as the total N, P, K and Zn contents in the flowers, roots, stems and leaves of the plants, were analysed. The balanced fertilization plan for cultivation of C. morifolium was put forward. In addition, the formula of special fertilizer for cultivation of C. morifolium was determined according to flower yield and utilization rate of N, P, and K.

RESULTS AND CONCLUSIONThe soil had high pH values and high soil salt contents, with unbalanced application of N, P, and K fertilizers and a shortage of available Zn after cultivation of C. morifolium. The contents of soil organic carbon, N and P declined with increasing cultivation time of C. morifolium, which resulted from the improper rotations and fertilization. The balance fertilization practice and the special fertilizer utilization are effective ways to improve soil fertility for C. morifolium.

Chrysanthemum ; chemistry ; growth & development ; Fertilizers ; Hydrogen-Ion Concentration ; Nitrogen ; analysis ; Phosphorus ; analysis ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; growth & development ; Potassium ; analysis ; Soil ; analysis ; Zinc ; analysis

OBJECTIVETo search an ideal method for treatment of severe blepharoptosis.

METHODSFifty-four eyes of 47 patients with severe blepharoptosis were undergoing for the treatment with a frontalis muscle complex flap, included in the frontalis muscle, orbicularis oculi muscle and SMAS membranes, to suspend the dropped eyelids.

RESULTSThe 54 eyes with severe blepharoptosis were successfully treated with the frontalis muscle complex suspension technique. Although the lagophthalmos in different degrees was shown in 3 months after the operation, it usually disappeared 6 months after the operation. The results were shown good appearance without recurrence.

CONCLUSIONThe above mentioned technique may be a good and effective method for treatment of the severe blepharoptosis, compared with the traditional technique.

Adolescent ; Adult ; Blepharoptosis ; surgery ; Child ; Female ; Humans ; Male ; Middle Aged ; Surgery, Plastic ; methods ; Treatment Outcome

OBJECTIVETo observe the post-injury regenerating ability of temporomandibular joint of rats exposed to the repeated + Gz forces.

METHODSOne hundred and eight male rats were randomly assigned to 9 groups, with 12 rats in each group. Group A was normally fed serving as the blank control group. Group B was only fixed with rat-kept devices for 5 minutes as the fixed control group. Group C was borne +1 Gz for 5 minutes. In the same position as group C, group D, E, F, G, H and I were repeatedly exposed to +10 Gz for 30 seconds each time with 5 times a day and +1 Gz for 1 minutes as a interval. The 6 groups were treated in the condition above for 4 days a week and 4 weeks in total. The rats in group A, B, C and D were killed on the next day after the centrifuge. The rats in group E, F, G, H and I were killed at 1, 2, 4, 8 and 12 weeks after the centrifuge, respectively. After the rats were killed, temporomandibular joint were taken for observation under optical microscope, scanning electron-microscope (SEM) and transmission electron-microscope (TEM).

RESULTSThere was no significant difference observed in group A, B and C. But in group D, E, F, G, H and I pathologic injuries and self-regeneration were seen in TMJ in different degrees.

CONCLUSIONSThe injury of TMJ could regenerate gradually with time.

Acceleration ; adverse effects ; Animals ; Male ; Rats ; Rats, Wistar ; Temporomandibular Joint ; injuries ; pathology ; ultrastructure