1.The analysis of polymorphism of red cell complement receptor type 1 density genomic in Olunchun nationality.
Hai-Bo JIA ; Hai-Ying JIA ; Lin SHI
Chinese Journal of Applied Physiology 2014;30(2):135-136
China
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Ethnic Groups
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genetics
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Female
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Humans
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Male
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Middle Aged
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Polymorphism, Genetic
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Receptors, Complement
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genetics
2.Case report: peritonsillar abscess with deep cervical space and mediastinal infection.
Hai-yan WANG ; Hai-ying JIA ; Hong LIANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2011;46(10):865-866
Adult
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Humans
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Male
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Mediastinitis
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complications
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Neck
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Peritonsillar Abscess
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complications
5.Breeding of High-yielding ?-galactosidase Strains from Protoplast of Aspergillus niger
Hai-Ying DU ; Hong-Wei YU ; Jun HAN ; Ning LI ; Ying-Min JIA ;
Microbiology 1992;0(06):-
The protoplasts of original Aspergillus niger strain Uco-3 were treated with the cooperation of UV and ?-ray to obtain the high-yielding strain producing the thermostable ?-galactosidase. Under the optimum conditions of formation and regeneration protoplasts were prepared. According to the interaction of positive mutation rate and radiation dose,the optimum condition was determined. The optimum dose of UV was 4 minutes and the optimum dose of ?-ray was 500 Gy. After mutagenetic treatment of protoplasts and selection from a lot of mutants,a mutant DL116 producing the thermostable ?-galactosidase was obtained. The ?-galactosidase activity of DL116 was increased from 16.27 U/mL to 44.37 U/mL,which was higher than that of strain Uco-3.
6.Immune Responses and Histopathological Changes in Rabbits Immunized with Inactivated SARS Coronavirus
Chuan-hai, ZHANG ; Xin-jian, LIU ; Yi-fei, WANG ; Jia-hai, LU ; Huan-ying, ZHENG ; Sheng, XIONG ; Mei-ying, ZHANG ; Qiu-ying, LIU
Virologica Sinica 2007;22(5):360-365
To evaluate the immunogenicity of inactivated SARS coronavirus (SARS-CoV), three groups of rabbits were immunized three times at 2-week intervals with inactivated vaccine + adjuvant, adjuvant,and normal saline respectively. Eight batchs of serum were sampled from the auricular vein at day 7 to day 51, and specific IgG antibody titers and neutralizing antibody titers were detected by indirect ELISA and micro-cytopathic effect neutralizing test. Antibody specificity was identified by proteinchip assay.Histopathological changes were detected by H&E staining. The results showed that, rabbits in the experimental group immunized with inactivated SARS-CoV all generated specific IgG antibodies with neutralizing activity, which suggested the inactivated SARS-CoV could preserve its antigenicity well and elicit an effective humoral immune responses. The peak titer value of specific IgG antibody and neutralizing antibody reached 1:40960 and 1:2560 respectively. In the experimental group, no obvious histopathological changes was detected in the H&E stained slides of heart, spleen, kidney and testis samples, but the livers had slight histopathological changes, and the lungs presented remarkable histopathological changes. These findings are of importance for SARS-CoV inactivated vaccine development.
7.The characteristics of vascular endothelial injuries induced by extreme environmental factors.
Wei LIU ; Jia-Ying LIU ; Zhao-Yun YIN ; Chao-Liang LONG ; Hai WANG
Chinese Journal of Applied Physiology 2013;29(6):494-500
Vascular endothelium plays an important role in regulating vascular homeostasis. Over the past years, it has become clear that endothelial dysfunction is a key event of pathophysiological changes in the initiation and progression of injuries induced by extreme environmental factors. The present review summarizes current understanding of vascular endothelial dysfunction induced by hypoxia, cold and heat, and provides the information for prevention and treatment of environmental exposure injuries.
Endothelium, Vascular
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physiopathology
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Environment
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Humans
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Hypoxia
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physiopathology
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Temperature
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Vascular System Injuries
8.Sperm acrosome formation-associated genes in mice: Advances in studies.
Chang-min NIU ; Jia-qian GUO ; Hai-tao MA ; Zhe ZHENG ; Ying ZHENG
National Journal of Andrology 2016;22(1):72-76
Spermiogenesis is a complex process of differentiation and morphologic alteration, in which sperm acrosome formation is an important stage. Acrosome is an essential component of the sperm head, which develops in four distinct phases: Golgi, cap, acro- somal, and maturation, each supported by precise and orderly regulation of various genes. The regulatory genes which act on Golgi ap- paratus include GOPC, Hrb, SPATA16, PICK1, and CK2α', those involved in the cap phase are Fads2, syntaxin 2, Kdm3a, and UBR7, and participating in acrosomal and maturation phases are KIFC1, Rnf19a, and DPY19L2. The abnormalities of these genes may affect male fertility by influencing the connection of the nuclear dense lamina and acroplaxome with the nuclear membrane and then the fusion and transportation of vesicles. This review focuses on the genes involved in different phases of acrosome formation.
Acrosome
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physiology
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Animals
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Golgi Apparatus
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Male
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Mice
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Sperm Head
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physiology
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Spermatids
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growth & development
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Spermatogenesis
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genetics
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Spermatozoa
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growth & development
9.Regulative effects of vessel active drugs on extremital skin temperature of experimental animals exposed to cold.
Xi LI ; Yu-lin AN ; Li ZHANG ; Zhong-hai XIAO ; Jia-Ying LIU ; Ying ZHANG ; Jun WANG ; Dan-feng YANG ; Hai WANG
Chinese Journal of Applied Physiology 2014;30(5):385-389
OBJECTIVEUsing an experimental model of animals exposed to cold to evaluate the regulative effects of prazosin hydrochloride (Pra) and racanisodamine (Ani) on extremital skin temperature of rats and mice.
METHODSEighty animals were randomly divided into eight groups according to the drug dosage. After been administered with drugs by intragastric at room temperature for 60 min, the animals were moved into specified temperature (5 degrees C,18 degrees C) environment and the skin temperatures at the 1/3 site at the proximal end of tail were measured by infrared camera on 180 min and 300 min. Effects of drug were evaluated by changes in tail skin temperatures.
RESULTSPra and Ani combination raised the extremital skin temperature of experimental animals significantly in a dose-dependent manner, while single use of Pra was not potent to rats and less potent to mice, and single use of Ani could not raise extremital skin temperature of both rats and mice. Change of rectal temperature in mice showed that Pra and Ani combination did not affect core temperature.
CONCLUSIONPra and Ani combination could significantly raise extremital skin temperature of rats and mice exposed to cold, and would not affect their core (rectal) temperature.
Animals ; Body Temperature ; Cold Temperature ; Mice ; Prazosin ; pharmacology ; Rats ; Skin Temperature ; drug effects ; Solanaceous Alkaloids ; pharmacology
10.Expression and characterization of protein disulfide isomerase-related protein A.
Hai-Ping ZHOU ; Cui-Juan JIA ; Yu-Ying ZHANG
Chinese Journal of Biotechnology 2004;20(4):595-600
Protein disulfide isomerase-related protein A (PRPA) was highly expressed (about 34%) in Escherichia coli by inserting the whole PRPA cDNA into the vector pET23b. After expression, the purified protein was acquired through ammonium fractional precipitation and Bio-Rex 70 chromatography. PRPA shows low disulfide isomerase activity (only about 1/250 of that of hPDI), decreases the reactivation yield of denatured and reduced lysozyme either in redox and non-redox Hepes buffer or redox PBS buffer and facilitates the aggregation of denatured and reduced lysozyme. Fluorescence spectra of PRPA indicate that PRPA has more hydrophobic groups at surface than that of hPDI, and which can be used to explain why PRPA has anti-chaperone activity during the refolding of denatured and reduced lysozyme.
Cloning, Molecular
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Fungal Proteins
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chemistry
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genetics
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isolation & purification
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Muramidase
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chemistry
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Plasmids
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Protein Folding
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Recombinant Proteins
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biosynthesis
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isolation & purification
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Spectrometry, Fluorescence