1.Studies on expression and activity of membrane in peripheral in blood cells in patients with acute coronary syndrome.
Wei-bin CAO ; Hai-yan LUO ; Xiao-hong HAO
Chinese Journal of Hematology 2013;34(3):264-266
Acute Coronary Syndrome
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blood
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Aged
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Cell Membrane
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metabolism
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Female
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Humans
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Male
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Middle Aged
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Thromboplastin
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metabolism
2.Recent advances in novel anticancer agents targeting β -catenin/TCF4 interaction for molecular cancer therapeutics
Zheng-hao FU ; Gan-gan YAN ; Hai-yan QI ; Xiao-ping LIU ; Yun-yu CHEN
Acta Pharmaceutica Sinica 2021;56(5):1238-1245
Wnt/
3.Differentiation Study of Chinese Medical Syndrome Typing for Diarrhea-predominant Irritable Bowel Syndrome Based on Information of Four Chinese Medical Diagnostic Methods and Brain-gut Peptides.
Hao-meng WU ; Zhi-wei XU ; Hai-qing AO ; Ya-fei SHI ; Hai-yan HU ; Yun-peng JI
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(10):1200-1204
OBJECTIVETo establish discriminant functions of diarrhea-predominant irritable bowel syndrome (IBS-D) by studying it from quantitative diagnosis angle, hoping to reduce interference of subjective factors in diagnosing and differentially diagnosing Chinese medical syndromes of IBS-D.
METHODSA Chinese medical clinical epidemiological survey was carried out in 439 IBS-D patients using Clinical Information Collection Table of IBS. Initial syndromes were obtained by cluster analysis. They were analyzed using step-by-step discrimination by taking information of four Chinese medical diagnostic methods and serum brain-gut peptides (BGP) as variables.
RESULTSClustering results were Gan stagnation Pi deficiency syndrome (GSPDS), Pi-Wei weakness syndrome (PWWS), Gan stagnation qi stasis syndrome (GSQSS), Pi-Shen yang deficiency syndrome (PSYDS), Pi-Wei damp-heat syndrome (PWDHS), cold-damp disturbing Pi syndrome (CDDPS). Of them, GSPDS was mostly often seen with effective percentage of 34. 2%, while CDDPS was the least often seen with effective percentage of 5.5%. A total of 5 discriminant functions for GSPDS, PWWS, GSQSS, PSYDS, and PWDHS were obtained by step-by-step dis- crimination method. The retrospective misjudgment rate was 4.1% (16/390), while the cross-validation misjudgment rate was 15.4% (60/390).
CONCLUSIONThe establishment of discriminant functions is of value in objectively diagnosing and differentially diagnosing Chinese medical syndromes of IBS-D.
Alarmins ; Brain ; Cluster Analysis ; Diarrhea ; classification ; diagnosis ; Hot Temperature ; Humans ; Irritable Bowel Syndrome ; classification ; diagnosis ; Medicine, Chinese Traditional ; Qi ; Retrospective Studies ; Surveys and Questionnaires ; Yang Deficiency
4.Solidification of volatile oil with graphene oxide.
Hong-Mei YAN ; Xiao-Bin JIA ; Zhen-Hai ZHANG ; E SUN ; Yi-Hao XU
Acta Pharmaceutica Sinica 2015;50(2):222-226
To evaluate the properties of solidifying volatile oil with graphene oxide, clove oil and zedoary turmeric oil were solidified by graphene oxide. The amount of graphene oxide was optimized with the eugenol yield and curcumol yield as criteria. Curing powder was characterized by differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The effects of graphene oxide on dissolution in vitro and thermal stability of active components were studied. The optimum solidification ratio of graphene oxide to volatile oil was 1:1. Dissolution rate of active components had rare influence while their thermal stability improved after volatile oil was solidified. Solidifying herbal volatile oil with graphene oxide deserves further study.
Calorimetry, Differential Scanning
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Clove Oil
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chemistry
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Curcuma
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chemistry
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Eugenol
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Graphite
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chemistry
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Microscopy, Electron, Scanning
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Oils, Volatile
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chemistry
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Oxides
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chemistry
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Plant Extracts
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chemistry
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Powders
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Sesquiterpenes
6.Study on porous starch as solid dispersion carrier of total Epimedium flavonoids.
Hong-mei YAN ; Xiao-bin JIA ; Zhen-hai ZHANG ; E SUN ; Yi-hao XU
China Journal of Chinese Materia Medica 2015;40(9):1723-1726
In order to evaluate the characteristic of porous starch (PS) as the solid dispersions carrier of the total Epimedium flavonoids (TEF), the PS was used. The dissolution of icariin was selected as an indicator to analyze the differences of dissolution between TEF and its solid dispersion. TEF was characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and X-ray powder diffraction (XRD). Solid dispersion was irregular block and no powder characteristics of TEF and PS could be seen in SEM, DSC and XRD analysis suggested that TEF may be present in solid dispersion as amorphous substance. The dissolution rate of icariin has been improved significantly when the proportion of TEF and PS was 1:2. PS as a traditional solid dispersion carrier is worthy of further study.
Calorimetry, Differential Scanning
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Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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chemistry
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Epimedium
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chemistry
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Flavonoids
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chemistry
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Porosity
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Solubility
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Starch
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chemistry
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X-Ray Diffraction
7.Effect of diammonium glycyrrhizinate on entecavir pharmacokinetics in rats.
Fei-Yan LI ; Hai-Ping HAO ; Kun HAO ; Ting-Ting YAN ; Guang-Ji WANG
Chinese Journal of Natural Medicines (English Ed.) 2013;11(3):309-313
AIM:
This study was designed to explore the effects of short-term and long-term pretreatment of diammonium glycyrrhizinate (GLN) on the pharmacokinetics of entecavir (ETV) in rats.
METHODS:
Male SD rats were randomized into short-term and long-term experimental groups, respectively. In the short-term experiment, the control group received saline, the low dose group received GLN 13.5 mg·kg(-1) and the high dose group received GLN 40.5 mg·kg(-1). ETV (0.09 mg·kg(-1)) was given i.g. 0.5 h after saline/GLN administration. For the long-term experiment, rats were allocated into two experimental designs. The control group received saline/ETV (0.09 mg·kg(-1)), the low dose group received GLN 13.5 mg·kg(-1)/ETV 0.09 mg·kg(-1) + GLN 13.5 mg·kg(-1), while the high dose group received GLN 40.5 mg·kg(-1)/ETV 0.09 mg·kg(-1) + GLN 40.5 mg·kg(-1); all administration was continued for 15 days. On the 16(th) day, 0.09 mg·kg(-1) ETV was administrated to all groups. Blood samples were obtained at different time points after ETV administration to determine plasma ETV concentrations.
RESULTS:
Pretreatment with glycyrrhizin resulted in no significant alterations in the main pharmacokinetic parameters of ETV in the short-term and long-term administration experiments.
CONCLUSION
Diammonium glycyrrhizinate has no effect on ETV pharmacokinetics in rats.
Animals
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Drug Interactions
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Glycyrrhizic Acid
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pharmacology
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Guanine
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analogs & derivatives
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blood
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pharmacokinetics
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Male
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Rats
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Rats, Sprague-Dawley
9.Identification and Analysis of Afe0329 Transcripton Relative to Copper Homeostasis in Acidithiobacillus ferrooxidans
Yan-Jie LUO ; He-Huan LIAO ; Cheng-Gui ZHANG ; Hao GUAN ; Hai-Lang LUO ; Xue-Duan LIU ; Guan-Zhou QIU ;
Microbiology 2008;0(10):-
With reverse transcriptase PCR, the transcripton of copper homeostasis relative gene Afe0329 in Acidithiobacillus ferrooxians standard strain ATCC23270 was investigated. The further analysis of genes in this transcripton was analyzed employed by Vector NTI, Blast, TMHMM Server, PSORTb software and so on. From the DNA of different strains, the transcripton of Afe0329 was amplified using special primer pairs to identify the universality of it in the genome of A.ferrooxidans strains. The results showed that gene Afe0330 and Afe0331 were cotranscribed with Afe0329, and they were in a single transcripton. Gene Afe0329 was supported to express a P1b3-type ATPase which is a heavy metal ion pumping transmembrane protein, protein AFE0330 which expressed by gene Afe0330 was a cytoplasmic protein, no significant ho- mologous sequences of Afe0330 or Afe0331 had been obtained by Blast analysis. And the transcripton of Afe0329 was universal in genome of A. ferrooxidans strains.
10.Pharmacokinetic comparison of baicalin absorption medicine Qinbai Qingfei concentrated pellets drug compatibility.
Hai-Long LI ; Wen-Cheng FENG ; Lin YAO ; Yan SUN ; Ya-Juan SONG ; Hao HU ; Wei-Ming WANG
China Journal of Chinese Materia Medica 2014;39(10):1933-1936
The Qinbai Qingfei concentrated pellets by traditional Chinese medicine theoryand party and group, the rats were given the drugs group, comparison of pharmacokinetics parameters changes of baicalin , discusses the rationality of Qinbai prescription. The rats were gavaged monarch drug group (Huang Qincu extract, mainly forbaicalin), and official medicine group, adjuvant group, medicine group and Qinbai group (Quan Fangzu) the content of baicalin equal as the monarch drug group, in the 28 h collection in rat plasma at different time point, application of HPLC determination of baicalin glycosides in rat plasmaconcentration time curve, with 3P97 practical pharmacokinetics program to process the data Based on the data analysis, baicalin in rat plasma of Qinbai group Cmax is 4 times as big as monarch druggroup, AUC is 6 times as big as monarch drug group; the content of baicalin in plasma of rats the highest is Qinbai group, the minister drug group, adjuvant group, medicine group of baicalin in rat plasma content of less than the Qinbai group, but was significantly higher than that of monarch drug group; the medicine group is slightly higher than that adjuvant the content of baicalin in plasma of rats. The pharmacokinetic results show that the measured plasma concentration in rats that Qinbai can significantly increase Cmax and AUC of baicalin, other components of qinbai can promoted the baicalin absorption in vivo. It showed that the reasonable of Qinbai compound compatibility. The minister drug can promote the absorption of baicalin in vivo.
Animals
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Area Under Curve
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Drugs, Chinese Herbal
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analysis
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pharmacokinetics
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Flavonoids
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blood
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pharmacokinetics
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Intestinal Absorption
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Male
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Rats
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Rats, Wistar