1.Biomarkers for diabetes prediction, diagnosis and personalized therapy.
Chinese Medical Journal 2012;125(23):4163-4166
2.Effect of Astilbin on expression of CTLA-4 in activated T cells of mouse heart transplantation model with acute rejection
Si-Hai GAO ; Ping LI ; Tao CHEN ;
Chinese Journal of Organ Transplantation 2005;0(10):-
Objective To investigate the effect of astilbin on expression of CTLA-4 in activated T cells of mouse heart transplantation model with acute rejection.Methods Cardiomyocytes of BALB/ c mouse and spleen cells of C57BL/6 mouse were separated.The cardiomyocytes(2?10~5/ml)as irri- tation cells and spleen cells(1?10~6/ml)as responsers were mixed and cultured.The model of mouse heart transplantation with acute rejection in vitro was established.Three groups were set up:control group,Astilbin(15/?g/ml) group,Astilbin+anti-CTLA-4 mAb 9H10 (30?g/ml)group.Apoptosis of T cells was observed by TUNEL.The expression of CTLA-4 in activated T cells was detected by RT-PCR and Western blot.Results Apoptosis indexes of activated T cells in Astilbin group were sig- nificantly higher than those in the control group(73.4%?12.5% vs 35.1%?9.2%,P<0.01). The expression of CTLA-4 in Astilbin group was significantly higher than control group(P<0.01), but there was no apparently difference between control group and Astilbin+CTLA-4 mAb group(P>0.05).Conclusion Astilbin induces apoptosis of activated T cells in heart transplantation,which may be partially related to its enhanced expression of CTLA-4.
5.Parotid gland carcinosarcoma: report of a case.
Hai-ping ZHANG ; Hai-fang ZHANG ; Pei-qiong CHEN
Chinese Journal of Pathology 2011;40(10):703-704
Carcinosarcoma
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metabolism
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pathology
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radiotherapy
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surgery
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Follow-Up Studies
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Humans
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Keratins
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metabolism
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Ki-67 Antigen
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metabolism
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Male
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Middle Aged
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Mucin-1
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metabolism
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Parotid Gland
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pathology
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surgery
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Parotid Neoplasms
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metabolism
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pathology
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radiotherapy
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surgery
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Radiotherapy, Adjuvant
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Vimentin
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metabolism
6.Retrospective study on volume of bone cement injection for concurrent of fracture after thoracolumbar vertebraes kyphoplasty.
Hui-Guo CHEN ; Jin-Ping CHEN ; Hai-Ping LIANG ; Qing-Zhou KONG ; Jian-Hong CHEN ; Ye ZHOU ; Zhe ZHANG
China Journal of Orthopaedics and Traumatology 2012;25(8):681-683
OBJECTIVETo explore relationship between volume of bone cement injection and concurrent of fracture after thoracolumbar osteoporotic vertebral fracture treated by percutaneous kyphoplasty (PKP).
METHODSFrom January 2006 to December 2008,68 patients with thoracolumbar osteoporotic vertebral fracture treated by PKP were retrospectively analyzed. Among them, 30 patients with less than 3 ml bone cement injection (mean 2.5 ml, low group), including 11 males and 19 females, with an average age of (85.0 +/- 8.5) years (ranging for 60 to 91); 38 cases with over 4 ml bone cement injection (mean 4.5 ml, large group), including 15 males and 23 females,with an average age of (86.0 +/- 9.2) years (ranging for 60 to 93). Factors of concurrent vertebral fractures were observed during follow-up.
RESULTSAll patients were followed up from 3.4 to 5.1 years with an average of 3.8 years. Thirteen patients (43.3%) co-occurred fracture in low group,among which strengthened concurrent vertebral fracture occurred in 1 case,upper and lower section adjacent vertebral fracture in 8 cases,distal segment of vertebral fracture in 4 cases; while 18 patients (47.3%) co-occurred fracture in large group,among which strengthened concurrent vertebral fracture occurred in 2 cases, upper and lower section adjacent vertebral fracture in 10 cases,distal segment of vertebral fracture in 6 cases. No significant difference between two groups (P > 0.05).
CONCLUSIONBone cement injection is not main influence factors for treating concurrent of fracture after thoracolumbar osteoporotic vertebral fracture by PKP. Concurrent fracture mainly relates with progress of osteoporosis, the volume of injection volume may appropriately over the volume of balloon.
Aged ; Aged, 80 and over ; Bone Cements ; Female ; Follow-Up Studies ; Humans ; Injections ; Kyphoplasty ; adverse effects ; Lumbar Vertebrae ; injuries ; surgery ; Male ; Middle Aged ; Osteoporotic Fractures ; etiology ; Postoperative Complications ; etiology ; Retrospective Studies ; Thoracic Vertebrae ; injuries ; surgery
7.Expression and significance of erythropoietin in human gastric carcinoma on tissue microarry.
Chen JIANG ; Jian-xian YU ; Hua CHEN ; Hong-jun WEI ; Hai-yan MA ; Ping JI
Chinese Journal of Pathology 2006;35(9):559-560
Adenocarcinoma
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metabolism
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pathology
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Adenocarcinoma, Mucinous
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metabolism
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pathology
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Adult
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Aged
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Aged, 80 and over
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Carcinoma, Signet Ring Cell
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metabolism
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pathology
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Erythropoietin
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metabolism
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit
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metabolism
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Immunohistochemistry
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Lymphatic Metastasis
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Microvessels
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chemistry
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pathology
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Middle Aged
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Stomach
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chemistry
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pathology
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Stomach Neoplasms
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metabolism
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pathology
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Tissue Array Analysis
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methods
8.Paneth cell-rich carcinoma of stomach: report of two cases.
Wei-dong SHI ; Chun-nian HE ; Jin-ping ZHAI ; Jin-hai SUN ; Chen CHEN
Chinese Journal of Pathology 2006;35(2):123-124
Adenocarcinoma
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pathology
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surgery
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Aged
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Carcinoma, Signet Ring Cell
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pathology
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surgery
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Cell Differentiation
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Female
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Follow-Up Studies
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Gastrectomy
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methods
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Humans
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Male
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Middle Aged
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Paneth Cells
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pathology
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Stomach Neoplasms
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pathology
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surgery
9.Clinical characteristics of nucleic acid negative neonates delivered by pregnant women infected with SARS-CoV-2 in Sanya, Hainan
FENG Hai-ping ; CHEN Xiao-bing ; CHEN Qiu-ning ; HUO Kai-ming ; ZHANG Xue-mei
China Tropical Medicine 2023;23(4):404-
Abstract: Objective To explore the clinical characteristics of nucleic acid negative newborns delivered by pregnant women infected with SARS-CoV-2 (Omicron variant BA. 5.1.3) in Sanya area, and to provide evidence for understanding its clinical characteristics. Methods A retrospective analysis was performed on 14 neonates with negative nucleic acid delivered by pregnant women who tested positive for SARS-CoV-2 (Omicron variant BA.5.1.3) in Sanya Central Hospital (the Third People's Hospital of Hainan Province) from June 2022 to September 2022 (observation group, n=14). The corresponding nucleic acid-negative newborns delivered by pregnant women detected negative with SARS-CoV-2 (Omicronon variant strain BA.5.1.3) were set as the control group (n=56), and the general data and clinical characteristics of neonates in the two groups were compared. Results There was no significant difference between the observation group and the control group in pregnancy diabetes, pregnancy induced hypertension, gestational pre-eclampsia, fetal intrauterine distress, premature rupture of membranes (P>0.05); there was no significant difference between the observation group and the control group in terms of sex, gestational age, birth weight, age, mode of delivery, birth Apgar score, heart screening, pulmonary disease, glucose 6-phosphate dehydrogenase (G6PD) deficiency, thalassemia, breast milk jaundice, hemolytic jaundice (P>0.05). The bilirubin level, blue light irradiation cases and the duration of blue light irradiation of the newborns in the observation group at 7 days after birth were higher than those in the control group (P<0.05); the ratio of blood oxygen saturation ≥ 90% in the observation group was lower than that in the control group (21.43% vs 89.29%, P<0.05), and the ratio of blood oxygen saturation occasionally<90% was higher than that in the control group (57.14% vs 10.71%, P<0.05). The ratio of blood oxygen saturation<90% had no significant difference compared with that in the control group (7.14% vs 0, P>0.05), and the ratio of blood oxygen saturation reduced to the required oxygen uptake was higher than that in the control group (14.29% vs 0, P<0.05). Conclusions The jaundice manifestation of the nucleic acid-negative newborns delivered by pregnant women infected with SARS-CoV-2 (Omicronon variant strain BA.5.1.3) in Sanya area is relatively obvious, with blood oxygen saturation easily lower than 90% and even requiring oxygen inhalation in severe cases.
10.Case-control study on Zero-profile implant for anterior cervical discectomy and fusion and conventional cage plate internal fixation for the treatment of single segmental cervical intervertebral disc herniation.
Hai-yu SHAO ; Jun ZHANG ; Di YANG ; Jin-ping CHEN ; Ya-zeng HUANG
China Journal of Orthopaedics and Traumatology 2016;29(6):530-537
OBJECTIVETo compare clinical efficacy of Zero-profile implant for anterior cervical discectomy and fusion and conventional titanium plate with cage internal fixation for the treatment of single segmental cervical intervertebral disc herniation.
METHODSFrom August 2011 to March 2014, clinical data of 139 patients with single cervical disc herniation treated with anterior cervical discectomy and interbody fusion with internal fixation were retrospectively analyzed. The patients were divided into two groups according to its operation method. There were 63 patients in group A which performed anterior discectomy and interbody fusion with Zero-profile;76 patients in group B which performed anterior cervical discectomy and cage plate internal fixation. JOA score and Odom functional rating between two groups were compared before and after operation. Videofluorographic swallowing study (VFSS) were used to evaluate thickness of prevertebral soft tissue. Bazaz dysphagia score were used to assess incidence of dysphagia. Postoperative AP X-ray and CT of cervical vertebra at 12 months were applied for evaluating bone graft fusion. Postoperative MRI was applied for evaluating the incidence of adjacent segment degeneration. Blood loss,operative time, preoperative and postoperative JOA score, Odom functional rating and VFSS score, Bazaz score, fusion rate between vertebral bodies and incidence of adjacent segment degeneration were compared between two groups.
RESULTSThere were no statistical meaning between two groups in JOA score, Odom functional rating before and after operation (P > 0.05); and no significant meaning in VFSS score between two groups before operation (P > 0.05); There were no significant difference in operative time and blood loss. There was statistical meaning in VFSS, Bazaz dysphagia score at 2 days, and 6 months after operation (P < 0.05). All patients obtained bone union at 1 year after operation, and no obvious meaning in fusion rate (P > 0.05). Eight patients (12.7%) in group A occurred adjacent segment degeneration and 19 patients (25%) in group B occurred adjacent segment degeneration, and there was significant meaning between two groups (P < 0.05).
CONCLUSIONBoth of Zero-profile implant for anterior cervical discectomy and fusion and conventional cage internal fixation for the treatment of single segmental cervical intervertebral disc herniation could obtain satisfied clinical results. While Zero-profile implant for anterior cervical discectomy and fusion has advantages of lower incidence of adjacent segment degeneration, and its mid and long term following-up results still further observation.
Adult ; Aged ; Bone Plates ; Case-Control Studies ; Cervical Vertebrae ; surgery ; Diskectomy ; Female ; Fracture Fixation, Internal ; Humans ; Intervertebral Disc ; surgery ; Intervertebral Disc Displacement ; surgery ; Male ; Middle Aged ; Retrospective Studies ; Spinal Fusion ; Treatment Outcome