OBJECTIVE: To investigate the effect and mechanism of Curcumenol against leukemia and to provide theoretical basis for the development and utilization of Curcumenol.METHODS:Curcumenol liposomes were prepared by mechanical dispersion method and ultrasonic method.MTT method was used to detect the inhibitory effects of Curcumenol and other drug on L1210 proliferation in mice with Lymphocytic leukemia.The apoptosis of L1210 cells induced by Curcumenol was observed under confocal microscope by trypan blue exclusion test and Hoechst 33258/PI double staining.RESULTS: Curcumenol significantly inhibited the proliferation of L1210 cells and induced apoptosis of L1210 cells.CONCLUSION: The anti-tumor effect of Curcumenol is possibly achieved by inducing the apoptosis of L1210 cells.

【Objective】To observe the jaundice-relieving effect of therapy of clearing heat and draining dampness combined with cooling blood and normalizing gallbladder function for severe icterohepatitis with damp-heat in liver and gallbladder.【Methods】Seventy-one severe icterohepatitis patients with damp-heat in liver and gallbladder were randomized into groups A(n=38) and B(n=33).The two groups received routine basic treatment and symptomatic treatment,group A was treated with heat-clearing,dampness-draining,blood-cooling and bile-draining herbs,and group B with intravenous drip of small-dose 654-2 additionally.Serum levels of total bilirubin(TBil),direct bilirubin(DBil),glutamic pyruvic transaminase(ALT),prothrombin activation(PTA) and jaundice duration were observed for the evaluation of jaundice-relieving effect.Meanwhile,the changes of gallbladder ultrasonograms were observed.【Results】After treatment,serum levels of TBil and ALT were decreased in both groups(P0.05)but the markedly effective rate in group A was higher than that in group B(P

Angiodysplasia ; Child, Preschool ; Humans ; Male ; Pulmonary Artery ; abnormalities ; Pulmonary Veins ; abnormalities

Humans ; Molecular Epidemiology ; Respiratory Syncytial Virus Infections ; epidemiology ; virology ; Respiratory Syncytial Virus, Human ; genetics

OBJECTIVETo discriminate and determine of the artificial bear bile of the compound bile capsule.

METHODTaking the pharmacopoeia as reference, the artificial bear bile was discriminated and determined by HPLC.

RESULTThe compound bile capsule and the control sample had chromatographic peak at the same time from HPLC. The content of the artificial bear bile was above 10 mg per tablets.

CONCLUSIONThe artificial bear bile of compound bile capsules can be discriminated effectively and determined accurately by HPLC method.

Animals ; Bile ; chemistry ; Capsules ; analysis ; Chromatography, High Pressure Liquid ; methods ; Discriminant Analysis ; Medicine, Chinese Traditional ; Taurochenodeoxycholic Acid ; analysis ; Ursidae

Objective To establish a method for the determination of Baicalin in Fuke Yangkun pills.Method High-performance liquid chromatography method was used.The chromatographic conditions were as follows:C18 column,mobile phase of methanol ∶0.4 %phosphoric acid(45 ∶55),detection wavelength being 280 nm,a flow rate of 1.20 mL?min-1 and the column temperature being 25 ℃.Results There existed a good linearity when the injection quantity is in the range of 0.0541 ?g to 0.5408 ?g.Average recovery is 99.98 %,and RSD is 1.32 %.Conclusion HPLC method is simple and effective and can be applied for the detemination of Baicalin in Fuke Yangkun pills.

Objective To investigate the feasibility and efficacy of tunica vaginalis flap cover in treatment of recurrent urethrocutaneous fistula (UCF).Methods We reviewed the clinical datas of 25 cases of UCF after urethroplasty of hypospadias from January 2011 to January 2015.The mean age of the patients was 6.2 years(range 1.6-14.0 years).All patients had undergone previous hypospadias repair and at least one previous failed attempt to close the urethrocutaneous fistulae.There were 5 cases of fistula in the coronary sulcus,6 cases of fistula in the penile body and 14 cases of fistula at the junction of penis and scrotum.The diameter of urethrocutaneous fistulae were 3-10 mm.Single fistula was present in all patients.16 cases had undergone UCF repair for two times, 9 cases had undergone UCF repair for more than two times.After closing the fistula with inverting running stitch, a tunica vaginalis flap was mobilized to cover the repair site through a subcutaneous tunnel and the skin closed.Results The mean follow-up time was 2.1 years(range 0.5-4 years).The overall success rate was 96％ (24/25).Penile cosmesis was excellent.There was no evidence of recurrent fistulas or urethral strictures.All parents reported a straight penis when erected, and one patient in whom there was leak from the fistula site because of local wound infection.No postoperative complication was encountered in the testis.The testis was of normal size and position, and the ultrasound findings were normal.Conclusions Repairment of recurrent urethrocutaneous fistulas with a tunica vaginalis flap could be effective regardless of fistula location.The technique is simple to improve the success rate of the repair of UCF effectively and reduce the occurrence of postoperative complications.

Hepatic artery stricture (HAS) after liver transplantation can lead directly to transplanted liver function exhaustion and complications of biliary system. The early diagnosis and treatment are crucial for better prognosis. Doppler ultrasound is the first method of choice, and angiography can give further clear dignosis. The balloon dilatation is still effective for hepatic arterial stenosis. With the more adaptable usage of oronary stent, if possible, would reveal more promising result especially for tortuous stenotic hepatic artery. The vascular reconstruction or repeated liver transplantation is still the effective therapeutic methods.

Adolescent ; Female ; Humans ; Jaw ; pathology ; Parathyroid Neoplasms ; complications ; pathology