1.Determination of tauroursodeoxycholic acid in compound bile capsule by HPLC.
Fei CHEN ; Hai-Lin LONG ; Hai-Min LEI ; Qiang LI
China Journal of Chinese Materia Medica 2014;39(5):838-840
OBJECTIVETo discriminate and determine of the artificial bear bile of the compound bile capsule.
METHODTaking the pharmacopoeia as reference, the artificial bear bile was discriminated and determined by HPLC.
RESULTThe compound bile capsule and the control sample had chromatographic peak at the same time from HPLC. The content of the artificial bear bile was above 10 mg per tablets.
CONCLUSIONThe artificial bear bile of compound bile capsules can be discriminated effectively and determined accurately by HPLC method.
Animals ; Bile ; chemistry ; Capsules ; analysis ; Chromatography, High Pressure Liquid ; methods ; Discriminant Analysis ; Medicine, Chinese Traditional ; Taurochenodeoxycholic Acid ; analysis ; Ursidae
2.Progress in the study of near-infrared fluorescent probes for the detection of β-amyloid deposition in Alzheimer's disease.
Lei DU ; Hai-wei FENG ; Yu-yan LI
Acta Pharmaceutica Sinica 2015;50(5):528-534
Alzheimer's disease (AD) is the most common cause of cognitive impairment in older people. With the aging of society is more and more serious, AD caused great burden to patients and society. A β is a classical biomarker of AD, which has been widely used in clinical diagnosis of AD patients. Compared with positron emission tomography (PET) and single photon emission computed tomography (SPECT), near infrared fluorescence imaging has many advantages including highly sensitive, non-invasive, safety and inexpensive. Therefore, many research groups have focused on developing the molecular probes of near-infrared fluorescence (NIRF) imaging. In this article, we will review the progress of the probes of NIRF.
Alzheimer Disease
;
diagnosis
;
Amyloid beta-Peptides
;
analysis
;
Fluorescence
;
Fluorescent Dyes
;
Humans
;
Molecular Probes
;
Positron-Emission Tomography
;
Spectroscopy, Near-Infrared
;
Tomography, Emission-Computed, Single-Photon
3.Detection on the pandemic( H1N1 ) 2009 virus of an influenza epidemic situation and study on the characteristic of HA and NA gene
Xia LEI ; Yan HAI ; Xin LI ; Weidong GUO
Chinese Journal of Microbiology and Immunology 2010;30(7):626-630
Objective To identify the pathogen of an influenza epidemic situation and analyze the genetic characteristic of hemagglutinin( HA ) gene and neuraminidase(NA) gene of this pathogen. Methods Real-time RT-PCR was used to dectect nucleic acid of the pandemic( H1N1 ) 2009 virus from oropharyngeal swabs of initial influenza-like illness in epidemic. The viruses were was inoculated and isolated with embryonated eggs. And the HA gene and NA gene were sequenced to analyze their characteristic. Results The influenza epidemic situation was caused by the pandemic( H1N1 ) 2009 virus. The HA and NA sequences data showed that the virus had the high homology with reference virus, and the NA sequences had not the H274Y mutation. Conclusion In this study, the pandemic( H1N1 ) 2009 virus were similar with the vaccine-like virus and the isolated virus of China, and sensitive to oseltamivir.
4.Detection and the Significance of SOCS-1 Gene Methylation Status in Peripheral Blood of Systemic Lupus Erythematosus Patients
Hai DING ; Shuo GAO ; Hongxia WEI ; Lei LI ; Qingfei WANG
Journal of Modern Laboratory Medicine 2017;32(1):34-37
Objective To investigate the relationship between the systemic lupus erythematosus (SLE)and the SOCS-1 gene methylation status of the peripheral blood DNA,to provide the basis for diagnosis and treatment of systemic lupus erythema-tosus.Methods Blood samples of SLE patients (27 cases)and healthy group (19 cases)in January 2015 to April were col-lected and the DNA were extracted.Using polymerase chain reaction combining DNA agarose gel electrophoresis to detect the SOCS-1 gene methylation status.Results In patients with systemic lupus erythematosus SOCS-1 gene complete methyl-ation accounted for 44% (12/27),incomplete methylation accounted for 56% (15/27).In healthy group SOCS-1 gene com-plete methylation accounted for 74% (14/19)and incomplete methylation accounted for 26% (5/19).The rate of complete methylation of SOCS-1 gene of SLE patients was lower than that of healthy group (χ2=3.88,P=0.049).Conclusion SLE patients may have lower SOCS-1 gene methylation status in the peripheral blood DNA,which is worth for further study.
5.HPLC enantioseparation, absolute configuration determination and anti-HIV-1 activity of (±)-F18 enantiomers.
Lei-lei ZHANG ; Hai XUE ; Li LI ; Xiao-fan LU ; Zhi-wei CHEN ; Gang LU
Acta Pharmaceutica Sinica 2015;50(6):733-737
Racemic (±)-F18 (10-chloromethyl-11-demethyl-12-oxo-calanolide A), an analog of nature product (+)-calanolide A, is a new anti-HIV-1 nonnucleoside reverse transcript inhibitor (NNRTI). A successful enantioseparation of (±)-F18 offering (R)-F18 and (S)-F18 was achieved by a chiral stationary phase prepared HPLC. Their absolute configurations were determined by measurement of their electronic circular dichroisms combined with modem quantum-chemical calculations. Further investigation revealed that (R)-F18 and (S)-F18 shared a similar anti-HIV activities, however, (R)-F18 was more potent than (S)-F18 against wild-type virus, K101E mutation and P225H mutation pseudoviruses.
Anti-HIV Agents
;
chemistry
;
Chromatography, High Pressure Liquid
;
HIV-1
;
drug effects
;
Pyranocoumarins
;
chemistry
6.Analysis of pesticides in blood specimen by GC/MS with accelerated solvent extraction.
Wen-hai LI ; Da-wei LIN ; Hong-lei SUN ; Maimaitituxun MUTAILIFU ; Lei WANG
Journal of Forensic Medicine 2014;30(6):463-465
OBJECTIVE:
To develop the accelerated solvent extraction (ASE) for determining pesticides present in blood samples.
METHODS:
Pesticides were extracted by ASE with optimized parameters to study recovery rate affected by extraction temperature, time and agent. GC/MS was used to perform quantitative analysis.
RESULTS:
The recovery rates of eight pesticides were 70.6%-92.4%. The coefficient of variation was less than 5.0%. A good linear relationship was obtained at the concentration range of 0.5-5.0 microg/mL.
CONCLUSION
The method was fast and simple with high recovery rate and good repeatability. It can be applied to analyze pesticides present in the blood specimen.
Chromatography, Liquid
;
Gas Chromatography-Mass Spectrometry
;
Pesticides/blood*
;
Solvents
;
Temperature
;
Time Factors
7.Clinical analysis and surgery of traumatic implantation cysts of the iris
Hai-Yan WANG ; Xiu-Qin PANG ; Bin LI ; Lan ZHANG ; Lei HE ; Shao-Li WANG ;
Ophthalmology in China 2006;0(06):-
Objective To explore the clinical characteristics, surgical treatment and related factors of traumatic implantation cyst of the iris. Design Retrospective cases series. Participants Thirty-six cases of traumatic implantation cyst of the iris. Methods Thir- ty-six cases of traumatic implantation cyst of the iris were reviewed. Main Outcome Measures Age, surgical history, history of disease, surgical method of patients. Results All cases of traumatic implantation cyst of the iris were secondary to perforating injury. 10 cases had been undertaken cataract surgery. The histories of disease in 6 months~1 year and 1 year~10 years were 30.56% respectively. Sur- gical exicision was taken in all cases. There were 2 recurrence cases. Conclusion Traumatic implantation cyst of the iris is almost see- ondary to perforating injury. Surgical excision is an effective strategy to treat this disease.
8.Effects of transforming growth factor-β2 on human Tenon fibroblasts transformation and scarring after glaucoma filtration surgery
Xiao-yan, ZHU ; Lei, LI ; Guang-jun, XIAN ; Hai-jun, LI ; Yan, TAN ; Lin, XIE
Chinese Journal of Experimental Ophthalmology 2013;(3):215-219
Background Research showed that transforming growth factor-β2 (TGF-β2) promotes scar formation.But its mechanism in scarring after glaucoma filtration surgery is worthy of studying.Objective This study was to investigate the effect of TGF-β2 on myofibroblast transition of human Tenon fibroblasts (HTFs) and scarring after glaucoma filtration surgery.Methods Tenon capsular tissue was obtained from 3 patients with strabismus during the surgery and was incubated in DMEM with 10% fetal bovine serum (FBS).The cells were collected and passaged in the free-serum medium for 24 hours,and then 1,2,5,10,20 μg/L TGF-β2 was added into the medium respectively,to induce the transformation of HTFs,and 2 μg/L or 5 μg/L TGF-β2 was used to treat the HTFs for 6,24,48 and 72 hours.The control group was not treated with TGF-β2.The expressions of α-smooth muscle actin (α-SMA) and phosphorylation of the signaling proteins (pSmad2) in HTFs were detected by Western blot assay.The expressions of α-SMA and F-actin were located by cell immunofluorescine technique under the confocal immunofluorescence microscopy.Cell contractility was determined by collagen gel contraction assays.This study was approved by Ethic Committee of Institute of Surgery Research of Daping Hospital,and informed consent was obtained from each patient or custodian initial of the study.Results The expression of α-SMA protein in the HTFs was increased significantly after the treatment of TGF-β2 in comparison with the control group and reached a peak at 24-48 hours.The α-SMA expression was gradually weakened in the 10 μg/L TGF-β2 groups.Little of α-SMA and F-actin were expressed in the control group.However,strong staining for α-SMA and F-actin were observed in the 1,2 and 5 μg/L TGF-β2 groups and then the staining weakened at the concentration of 10 μg/L.In addition,pSmad2 showed a stronger expression in the 2 μg/L TGF-β2 group than that in the PBS group and FBS group,with the strongest expression in 30 minutes through 2 hours.The untreated gel contracted (78.00±3.13)% from its initial size,and contraction in the 1,2,5,10 μg/L TGF-β2 group were (63.88±1.78)%,(20.69±0.65)%,(19.49-±0.54)%,(16.24±0.84) %,respectively,TGF-β2 increased HTFs contraction significantly (Fgroup =859.400,P =0.000).Conclusions TGF-β2 can induce transdifferentiation of Tenon fibroblast into myofibroblast and increase cell contractility,with a concentration-dependent and time-dependent pattern to an extent.It may be the mechanism of scar formation after glaucoma filter surgery.
9.The evaluation of efflux transporter model based on RNA interference technology in vitro.
Ling-lei KONG ; Hai-ying YANG ; Mei YUAN ; Xiao-mei ZHUANG ; Hua LI
Acta Pharmaceutica Sinica 2015;50(9):1122-1127
In the present study, the specifically knockdown models of P-gp or MRP2 were constructed by using a series of chemically synthesized small interfering RNA (siRNA) in vitro. The expression of P-gp and MRP2 was measured by real-time PCR and Western blot, and the function was evaluated by applying P-gp and MRP2 substrate, rhodamine and methotrexate. The results showed that MRP2 siRNA-3 or P-gp siRNA-2 significantly decreased the mRNA expression of MRP2 or P-gp, the inhibition ratio was 68% or 84%; MRP2 siRNA-3 or P-gp siRNA-2 at a dose of 80 nmol x L(-1) significantly reduced the protein expression of MRP2 or P-gp at 48 h after treatment, the inhibition ratio was 62% or 70%. Meanwhile, other transporters were not influenced by siRNA. When pretreatment with MRP2 siRNA-3 or P-gp siRNA-2, the efflux of methotrexate or rhodamine decreased significantly and the intra-cellular concentration increased. The results suggested that chemically synthesized siRNA could significantly inhibit the expression and function of MRP2 and P-gp, and the model of RNAi in vitro could be used to evaluate the role of efflux transporters in transportation of drugs.
ATP-Binding Cassette, Sub-Family B, Member 1
;
genetics
;
Gene Knockdown Techniques
;
Multidrug Resistance-Associated Proteins
;
genetics
;
RNA Interference
;
RNA, Small Interfering
;
Real-Time Polymerase Chain Reaction
10.The Value of 64-slice Spiral CT Digital Subtraction Technique in the Diagnosis of Intracranial Aneurysm
Zengcun SU ; Guangrui SHAO ; Fei LI ; Hai ZHONG ; Lei ZHAO ; Kun ZHAO
Journal of Practical Radiology 2009;25(12):1703-1706
Objective To study the value of 64-slice spiral CT digital subtraction technique in the diagnosis of intracranial aneurysms.Methods 36 patients with subarachnoid hemorrhage underwent 64-slice spiral CT examination,including unenhanced CT scan and enhanced CT scan.The image data were used to reconstruct the conventional CTA and digital subtraction CTA(DSCTA).Then the differences of image quality and diagnostic efficiency between the CTA and DSCTA were compared based on the standard of DSA . Results There were 40 intracranial aneurysms in 36 patients . 40 aneurysms were detected completely by DSCTA,the sensitivity and specificity of the DSCTA were both 100% . 37 aneurysms were detected by CTA , and the sensitivity and specificity were 92.5% and 100%,respectively.There was significant difference in image quality between CTA and DSCTA(P<0.01).Conclusion DSCTA with 64-slice spiral CT might be the first diagnostic tool in screening intracranial aneurysm.