Adolescent ; Alopecia ; diagnosis ; therapy ; Bone and Bones ; abnormalities ; Denture, Complete, Upper ; Diarrhea ; diagnosis ; therapy ; Female ; Humans ; Spasm ; diagnosis ; therapy

Objective To investigate biomechanical analysis of poly DL-lactic acid (PDLLA)biodegradable fusion cage of the lumbar vertebrae in vivo degradation. Methods Forty-eight healthy adult porcine were divided into experimental group and control group at randomly. PDLLA cage full of autogenous bone and bone block with the same size were planted in L45 intervertebrae respectively. At the 1st, 3rd, 6th,9th, 12th, and 18th month postoperatively, the animals were sacrificed and the surgical spinal segment was observed. The range of motion (ROM) was measured. Results The ROMs had no statistical differences at all motion directions between the two froups at the 1st month postoperatively. But the ROMs of experimental group were greater than those of control group at extension direction at the 3rd and 6th month postoperatively. The ROMs of extension was the greatest while the ROMs of right rotation was the least in both groups. At the 9th month postoperatively: the ROMs of experimental group greater than those of control group except at rotation. The ROMs of right rotation was the least in both groups. The greatest ROMs was at extension in experimental group and at right bending in control group. ALL ROMs tended to decrease and the ROMs of extension had comparability between tow groups. At the 12th month postoperatively, experimental group had greater ROMs at extension and left bending and less ROMs at other directions. Likewise statistical differences showed only at extension. 18th month postoperatively all ROMs were close between two groups. Conclusion After PDLLA cage is planted, the first 3 months is autologous stable stage. The period of 3rd to 6th months is easily released stage. The operative segments are stable from 9th to 12th months and acquired.Critical period between stability and instability is from 6th to 9th months. Bone fusion is from 12th to 18th months postoperatively.

BACKGROUND:Posterior lumbar interbody fusion is a typical therapeutic method of lumbar degenerative disease. Present studies suggested that adjacent segment degeneration occurs after fusion. Recently, more and more scholars paid attention to the development of non-fusion of the spine. ＜br> OBJECTIVE:To compare clinical effects of Dynesys dynamic stabilization system fixation and posterior lumbar interbody fusion in treatment of lumbar degenerative disease. ＜br> METHODS:From July 2009 to July 2011, clinical data of 56 patients with lumbar degenerative disease were retrospectively analyzed. There were 28 cases of Dynesys dynamic stabilization system fixation, and 28 cases of posterior lumbar interbody fusion. Operation time, bleeding volume, and postoperative hospitalization time were compared in both groups. Visual analog scale was used to assess pain. Oswestry disability index was utilized to evaluate clinical effects. ＜br> RESULTS AND CONCLUSION:A total of 56 patients were fol owed up for 18-24 months. Visual analog scale and Oswestry disability index scores were significantly improved at 12 months after treatment in both groups (P＜0.01). Significant differences in operation time, bleeding volume, and postoperative hospitalization time were detected between both groups (P＜0.01). Dynesys dynamic stabilization system group was better than posterior lumbar interbody fusion group. Range of motion was better in the Dynesys dynamic stabilization system group than in the posterior lumbar interbody fusion group (P＜0.01). During fol ow-up, nail rope system and polyester sleeve loose were not detectable in the Dynesys dynamic stabilization system group. These results verified that compared with posterior lumbar interbody fusion, Dynesys dynamic stabilization system for lumbar degenerative diseases has a high safety and smal trauma, and can keep advantages of a fixed segment, and exert a certain effect on degeneration of intervertebral disc in the adjacent segment.

BACKGROUND:Metal vertebral fusion cage can induce some disadvantages and pseudoarticulation formation such as stress dodge,fusion cage subsidence,bone resorption,prolapse and bone collapse.Biodegradable absorbing material-made vertebral fusion cage can avoid disadvantages mentioned above.There are no studies on correlation of material function to design of absorbing fusion cage of lumbar vertebrae.OBJECTIVE:To design and fabricate the poly DL-lactic acid biodegradable fusion cage of lumbar vertebrae,and to analyze the material characteristics.DESIGN,TIME AND SETTING:The material in vivo experiment was performed at the Chengdu Institute of Organic Chemistry of Chinese Academy of Sciences from January 2003 to December 2006.MATERIALS:Twenty dogs aged 12 months were obtained from Animal Laboratory of West China,Sichuan University.Poly DL-lactic acid biodegradable fusion cage of lumbar vertebrae was produced by the Chengdu Institute of Organic Chemistry of Chinese Academy of Sciences.METHODS:According to the anatomical characters and measured data of lumbar intervertebral disc,we designed the poly DL-lactic acid biodegradable fusion cage of lumbar vertebrae.The rectangle cage consists of two styles:23 mm and 25 mm,13 mm in width,and 9,10,11 and 12 in depth(anterior wall).The depth was small half-moon shape from anterior to posterior,and the posterior wall was thinner than anterior wall about 1.5mm to accord with the shape of intervertebral discs.The depth of four walls of the fusion cage was 2.5 mm,with several 1-mm diameter holes on bilateral walls.The posterior wall had 3.5-mm diameter spiral hole,which was used for connecting with the implanted tool.Using precise shaping processing craft,the produce process was DL-lactic acid→raw material storage→lactide preparation→lactide purification→polymerization→drying package→product.After general anesthesia,bilateral thighs were incised in 20 dogs,and then a fusion cage was implanted into each dog to detect compressive strength and viscosity in the process of biodegradation.MAIN OUTCOME MEASURES:Preparation of fusion cage;compressive strength and viscosity in the process of biodegradation in vivo.RESULTS:The size of the products is 23 mm?13 mm?10 mm and the appearance of the products were saw tooth shape,which could prevent the slide,enhance the solid strength between fusion cage and the surface of vertebra.Small holes on the bilateral wall were beneficial for vascular growth and nutrient fluid exchange,resulting in bony fusion.Compressive strength and viscosity decreased at 3,6 and 9 months after implantation(r=0.961,P=0.009),but compressive strength and viscosity were good at 12 months(163 MPa and 0.66 dl/g).CONCLUSION:Poly DL-lactic acid biodegradable fusion cage has a big loading implanted bone mass and high stability.The integrity and supportive effect of the fusion cage can meet the requirement of bony fusion.

Objective To explore the value of temporary cardiac pacemaker therapy on children with Adam-Stoke syndrome(ASS).Methods Nine children with ASS was implanted the emporary cardiac pacemaker under X-ray or blinding inserted beside bed.Results Seven children was successfully implanted the temporary cardiac pacemaker within 1-2 d of ASS attack,and two children was successfully implanted at 10 d and 20 d after ASS attack, respectively. Five patients was cured, two children was died, and two children was implanted the permanent cardiac pacemaker. During pacing, two patients had the electrode shifted,and one patient had the cardiac murmur,and one patient had the local skin infected.Conclusion Temporary cardiac pacemaker can successfully treat children with ASS attack;the earlier the implantation,the better the prognosis.

OBJECTIVETo observe the effect of β₃adrenoceptors (β₃-AR) activation on rat thoracic aorta smooth muscle contractility and the possible related mechanism.

METHODSThe endothelium removed thoracic aorta was pre-contracted with 30 mmol/L KCl physiological saline solution (PSS). Then the tension of the thoracic aorta was recorded in presence of BRL37344 (BRL) to determine the action of β₃-AR. The tension of the thoracic aorta was also recorded in the presence of Propranolol (PRA), SR59230A (SR), L-NNA, H-89 and Iberiotoxin (IBTX) respectively to reveal the underling mechanism of β₃-AR activation on rat vascular smooth muscle. Immunohistochemistry was adopted to confirm the existence and the distribution of β₃-AR in rat thoracic aorta.

RESULTSThe results showed that: (1) The thoracic aorta was relaxed by β₃-AR activation, with a relaxation percentage of (10.59 ± 0.79). (2) β₃-AR was expressed in both endothelial and smooth muscle layer in thoracic aorta sections of rats. (3) PRA did not block the effect of BRL on the thoracic aorta. The relaxation actions of BRL could be antagonized by pre-incubating the thoracic aorta with SR. (4) L-NNA (a NOS inhibitor) and H-89 (a PKA inhibitor) reversed the relaxation effect of BRL on vascular smooth muscle. (5) The effect of BRL was decreased after application of Ibriotoxin (IBTX), a large conductance calcium dependent potassium channel blocker.

CONCLUSIONThe results confirmed that activation of β₃-AR led to relaxation of thoracic aorta smooth muscle. The relaxation action of β₃-AR on smooth muscle of rat thoracic aorta was related to activation of NOS and PKA signaling pathway. Large conductance Ca²⁺-K⁺ channels were involved in the relaxation action of β₃-AR activation on rat thoracic aorta smooth muscle.

Animals ; Aorta, Thoracic ; physiology ; In Vitro Techniques ; Isoquinolines ; Large-Conductance Calcium-Activated Potassium Channels ; physiology ; Muscle Contraction ; Muscle Relaxation ; Muscle, Smooth, Vascular ; physiology ; Nitroarginine ; Peptides ; Propanolamines ; Propranolol ; Rats ; Receptors, Adrenergic, beta-3 ; physiology ; Signal Transduction ; Sulfonamides

To observe the effects of Danshen-containing serum on SuFu and DYRK2 expression in the HSCs stimulated by leptin. SD rats (n = 60) were used to make danshen-containing serum by gastric perfusion for ten days with Danshen water decoction, normal saline and colchicine. The HSCs that were cultured in vitro would be stimulated for 24 hours by leptin (100 μg x L(-1)) except blank control group, after being intervened, the drug serum in each group would be cultured at 37 degrees C in 5% incubator. The cells would be collected after 24 hours, then the effects of danshen-containing serum on the proliferation of HSCs were detected by MTT, the expression of SuFu mRNA and DYRK2 mRNA were detected by RT-PCR, the expression of SuFu and DYRK2 proteins were tested by Western blot. Compared with blank control group, the expression of DYRK2 mRNA and DYRK2 proteins were enhanced obviously after stimulated the HSCs of rats by leptin (P < 0.01), but the expression of SuFu mRNA and SuFu proteins were decreased significantly (P < 0.01). Compared with the model group, after cyclopamine group (Hh pathway inhibitor), Danshen-containing serum and colchicine were interfered, the expression of DYRK2 mRNA and DYRK2 proteins were decreased clearly (P < 0.01), but the expression of SuFu mRNA and SuFu proteins were increased significantly (P < 0.01 or P < 0.05). Compared with model group, adding purmorphamine (Hh pathway agonist) to model group and making it activate could increase the expression of DYRK2 mRNA and DYRK2 proteins, but the expression of SuFu mRNA and SuFu proteins were decreased significantly (P < 0.01). Compared with the model group, using the Danshen-containing serum to interfere the purmorphamine group could make the expression of DYRK2 mRNA and DYRK2 proteins decrease and the expression of SuFu mRNA and SuFu proteins increase significantly (P < 0.01). Danshen-containing serum would inhibition the activation and increment of HSCs by interfering the expression of SuFu and DYRK2 which were induced by leptin.
Animals ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Hepatic Stellate Cells ; drug effects ; metabolism ; Humans ; Liver Cirrhosis ; drug therapy ; genetics ; metabolism ; Male ; Protein-Serine-Threonine Kinases ; genetics ; metabolism ; Protein-Tyrosine Kinases ; genetics ; metabolism ; Rats ; Rats, Sprague-Dawley ; Repressor Proteins ; genetics ; metabolism ; Salvia miltiorrhiza ; chemistry

Colitis, Ulcerative ; complications ; drug therapy ; Humans ; Male ; Middle Aged ; Psoriasis ; complications ; drug therapy ; Pyoderma Gangrenosum ; drug therapy ; etiology

Objective To explore the clinical characteristics, surgical treatment and related factors of traumatic implantation cyst of the iris. Design Retrospective cases series. Participants Thirty-six cases of traumatic implantation cyst of the iris. Methods Thir- ty-six cases of traumatic implantation cyst of the iris were reviewed. Main Outcome Measures Age, surgical history, history of disease, surgical method of patients. Results All cases of traumatic implantation cyst of the iris were secondary to perforating injury. 10 cases had been undertaken cataract surgery. The histories of disease in 6 months～1 year and 1 year～10 years were 30.56% respectively. Sur- gical exicision was taken in all cases. There were 2 recurrence cases. Conclusion Traumatic implantation cyst of the iris is almost see- ondary to perforating injury. Surgical excision is an effective strategy to treat this disease.