1.Association of Human Leukocyte Antigen Allele Polymorphism and Susceptibility to Children with Acute Lymphocytic Leukemia
jun, HONG ; hong, SHEN ; hai-xia, CAO
Journal of Applied Clinical Pediatrics 2006;0(15):-
Objective To explore the possible association between human leukocyte antigen(HLA) allele polymorphism and susceptibility to chlidren's acute lymphocytic leukemia(ALL).Method HLA alleles polymorphism in 38 cases with ALL and 35 healthy controls were determined by polymerase chain reaction(PCR) with sequence specific olignuleotide probe(PCR/SSO).Results The allele frequency of HLA-A01 significantly increased than control group(?2=4.947 P=0.026,RR=10.20),the frequence of A02 significantly decreased(?2=4.187 P=0.041,RR=3.13),the frequcnce of A33 significunt decreased than control group(?2=4.403 P=0.036,RR=0.21).Conclusion These results show that susceptibility to chlidren's ALL is positively related to HLA-A01,A33,especially A01 allele,while HLA-A33 to its genetic resistance.
2.Expression of Truncated NSP2 Protein of Porcine Reproduction and Respiratory Syndrome Virus in E.coli and Preparation of Monoclonal Antibodies Against NSP2 Protein
Hai-Yan WANG ; Ping JIANG ; Yi-Jun DU ; Yu-Feng LI ; Jun-Xing LI ; Fang SHEN ;
China Biotechnology 2006;0(02):-
The NSP2 gene of porcine reproductive and respiratory syndrome virus (PRRSV)S1 strain was partly amplified and cloned into a prokaryotic expression vector pGEX-6P-1 and a fusion protein GST-tNSP2 with molecular weight of 50 kDa was expressed in E.coli. The purified GST-tNSP2 protein showed a strong reaction with the PRRSV-positive sera in Western blot assay. Balb/c mice were immunized with the purified protein, and the splenocytes of the immunized mice were fused with murine myeloma cells SP2/0. After subcloning by 3 times, two hybridoma clones which produced McAbs steadily were screened by ELISA, named 3H3 and 2B5. They all reacted strongly with the PRRSV S1 infected Marc-145 cells in IFA, but not with the PRRSV SY0608 strain. Both of the McAbs belong to IgG1 isotype, and their light chains belong ? type. The expressed GST-tNSP2 protein and McAbs could be used for identification of PRRSV isolates and functional analysis of NSP2.
3.Evaluation of bubble oxygen inhalators' performances and an investigation on their solutions for improvement.
Mian-kang CHEN ; Zheng-hai SHEN ; Xun-liang XU ; Jun-cheng BAO ; Chang-shan ZUO ; De-jun TANG ; Jun YANG
Chinese Journal of Medical Instrumentation 2007;31(4):295-296
This paper analyses the defects of bubble oxygen inhalators currently used, and investigates into their solutions for improvement.
Oxygen Inhalation Therapy
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instrumentation
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methods
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Oxygenators
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standards
4.Molecular simulations of the loading of methadone and buprenorphine into carbon nanotubes.
Acta Pharmaceutica Sinica 2006;41(9):888-892
AIMTo simulate the inhalation of the C21H27NO and C29H41NO4 molecules, the effective components of methadone and buprenorphine, into carbon nanotubes, and discuss the feasibility of the loading of methadone and buprenorphine into carbon nanotubes.
METHODSThe MM + force-field based molecular dynamics (MD) method uas used.
RESULTSThe ends-opened carbon nanotubes with diameter larger than 1 or 1.25 nm can initiatively inhale the C21H27 NO or C29H41NO4 molecule, and both two molecules have higher potential energy at the open ends of the carbon tubes than that at the middle of the tubes; the present single-walled nanotubes are very suitable for the loading of methadone and buprenorphine.
CONCLUSIONIt is possible to make sustained-release detoxification agents with methadone- or buprenorphine-loaded carbon nanotubes.
Buprenorphine ; chemistry ; Computer Simulation ; Methadone ; chemistry ; Models, Molecular ; Molecular Structure ; Nanotubes, Carbon ; chemistry ; Narcotic Antagonists ; chemistry
5.Expression of NSP 3AB Gene of Encephalomyocarditis Virus(EMCV)in E.coli and Development of Monoclonal Antibodies Against 3AB Protein
Fang SHEN ; Ping JIANG ; Yu-Feng LI ; Jun-Xing LI ; Hai-Yan WANG ;
China Biotechnology 2006;0(09):-
Objective:To express the EMCV 3AB gene by prokaryotic expression systerm,and prepare monoclonal antibodies against it. Method: NSP 3AB gene of Encephalomyocarditis virus (EMCV) was amplified and cloned into a prokaryotic expression vector pGEX-6P-1 and a recombinant protein 3AB with high antigenicity was expressed in E.coli. Balb / c mice were immunized by purified recombinant 3AB protein of inclusion-body, and the splenocytes of the immunized mice were fused with murine myeloma cells to produce hybridoma cell line. Results: After subcloning by 3 times, one strain of hybridoma cell line steadily secreting antibodies of 3AB protein was obtained, named 2D12. The McAb belongs to IgG1/?. The McAb and was confirmed by indirect immunofluorescent assay (IFA) and Western blot. Conclusion: These results can provide a potential value for structural and functional studies of EMCV-3AB and early diagnosis of Encephalomyocarditis virus infection.
6.Expression and role of the inducible costimulator (ICOS) in patients with rheumatoid arthritis
Qing CAI ; Jun ZHANG ; Yu LIU ; Lan-Ling ZHANG ; Xing-Hai HAN ; Qian SHEN ;
Chinese Journal of Rheumatology 2003;0(11):-
Objective To assessed the expression of inducible costimulator(ICOS)on peripheral blood and joint fluid CD4,CDS,CD45RO T cells and B cells in rheumatoid arthritis(RA).Methods Expression of ICOS and ICOS/CD45RO on peripheral blood and joint fluid CD4~+CD8~+T cells and ICOS ligand(ICOSL)on CD19 B cells from RA patients and healthy volunteers were determind by three-color flow cytometry.Compar- ision with active and inactive RA,initial and relapsed RA had been done.Results Joint fluid CD4 and CD8 T cells expressing ICOS,ICOS/CD45RO were significantly increased than peripheral blood in RA patients and healthy subjects.Joint fluid B cells expressing ICOSL were significantly reduced than peripheral blood in RA patients.Meanwhile,peripheral blood B cells expressing ICOSL were significantly reduced in active RA than inactive RA patients.Conclusion Hyperexpression of ICOS and ICOS/CD45RO on joint fluid CD4 and CD8 T cells and lowexpression of ICOSL in B cells from RA patients,expecially in active RA may contribute to the local immunopathological roles and joint destructions in the pathogenesis of RA.
7.Surgical treatment of poor grade middle cerebral artery aneurysms associated with large sylvian hematomas following prophylactic hinged craniectomy.
Hai-Jun, WANG ; You-Fan, YE ; Yin, SHEN ; Rui, ZHU ; Dong-Xiao, YAO ; Hong-Yang, ZHAO
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(5):716-21
The clinical characteristics of patients who presented in poor clinical grade due to ruptured middle cerebral artery aneurysms (MCAAs) associated with large sylvian hematomas (SylH) were analyzed and an ingenious designed prophylactic hinged craniectomy was introduced. Twenty-eight patients were graded into Hunt-Hess grades IV-V and emergency standard micro-neurosurgeries (aneurysm clipping, hematoma evacuation and prophylactic hinged craniectomy) were performed, and their clinical data were retrospectively analyzed. 46.43% of the patients reached encouraged favorable outcomes on discharge. The favorable outcome group and the poor outcome group significantly differed in terms of patients' anisocoria, Hunt-Hess grade before surgery, extent of the midline shift and time to the surgery after bleeding (P<0.05). There were no significant differences in age, sex, volume and location of the hematoma, size of aneurysm between the favorable and poor groups (P>0.05). However, ingenious designed prophylactic hinged craniectomy efficiently reduced the patients' intracranial pressure (ICP) after surgery. It was suggested that preoperative conditions such as Hunt-Hess grading, extent of the midline shift and the occurrence of cerebral hernia affect the prognosis of patients, but time to the surgery after bleeding and prophylactic hinged craniectomy are of significant importance for optimizing the prognosis of MCAA patients presenting with large SylH.
8.Effect of Hematopoietic Growth Factors in Placenta Chorionic Villi and Umbilical Cord Blood on Placenta Hematopoiesis
hui, YAO ; hai-yan, XU ; bo-jun, SHEN ; xiao-ling, BAI ; jin -guang, CAI ; qing, SHI
Journal of Applied Clinical Pediatrics 1992;0(06):-
Objective To study the role of hematopoietic growth factor(HGF)of placenta chorionic villus in fetal hematopoiesis during embryo ontogeny by observation of the appearance time and the content changes with the fetal growth, which was compared with HGF in cord blood. Methods Thirty embryo villus (2 g each) and 30 cord blood (2 mL each) were collected separately from early pregnant stage(6- 8 weeks), middle pregnant stage(16-22 weeks)and late pregnant stage (37-42 weeks). The levels of HGF were detected by enzyme - linked immunosorbent assay. Results HGF were produced on the early pregnant stage and the content of FL-T3,IL-3 increased gradually.There were significantly differences at different stages(P
9.Hollow copper sulfide nanoparticles loading deferoxamine for photothermal antibacterial therapy and promoting angiogenesis
Yi QIAO ; Chun ZHANG ; Yan-e MA ; Jia-ling CHEN ; Hai-jun SHEN
Acta Pharmaceutica Sinica 2023;57(9):2794-2801
Diabetic ulcer is recognized as a chronic nonhealing wound, often associated with bacterial infection and tissue necrosis, which seriously affect patients' health and quality of life. The traditional treatment methods exist some problems, such as bacterial resistance and secondary trauma, so it is urgent to find new methods to meet the requirements of diabetic ulcer treatment. In this study, we prepared a drug delivery system (DFO@CuS nanoparticles) based on hollow copper sulfide (CuS) nanoparticles loaded with deferoxamine (DFO), which realized the synergistic therapy of promoting angiogenesis and photothermal antibacterial. The morphological structure and particle size distribution of DFO@CuS nanoparticles were characterized by transmission electron microscopy and particle size analyzer, respectively. The antibacterial effect of DFO@CuS nanoparticles was evaluated by the plate coating method. The effects of DFO@CuS nanoparticles on the proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) were evaluated by CCK-8 (cell counting kit-8) assay, cell scratch assay, and tube formation assay. The results showed that DFO@CuS nanoparticles were hollow and spherical in shape with an average particle size of (200.9 ± 8.6) nm. DFO@CuS nanoparticles could effectively inhibit the growth of methicillin-resistant
10.Inhibitory effect of Panax notoginseng saponins on alveolar epithelial to mesenchymal transition.
Zhou-xin REN ; Hai-bin YU ; Jian-sheng LI ; Jun-ling SHEN ; Jun-kai LI ; Shan LUO
China Journal of Chinese Materia Medica 2015;40(23):4667-4671
In the study, the effects of Panax notoginseng saponins (PNS) on alveolar epithelial to mesenchymal transition (EMT) and extracellular matrix degradation were observed in a type of human alveolar epithelial cell, A549 cells, stimulated by TGF-beta1. Firstly, MTT method was applied to evaluation of cellular proliferation and found that PNS from 12.5 mg x L(-1) to 200 mg x L(-1) dosage could not inhibit significantly cellular proliferation. Then, cells were divided into five groups, normal group, TGF-beta1 group, TGF-beta1 + 50 mg x L(-1) PNS group, TGF-beta1 + 100 mg x L(-1) PNS group and TGF-beta1 + 200 mg x L(-1) PNS group. Normal cells were not stimulatec by TGF-beta1; TGF-beta1 cells were only stimulated by TGF-beta1 and the other cells were stimulated by TGF-beta1 with different doses of PNS, respectively. After stimulation, cells and supernatants were collected for assays. Cellular roundness was applied to quantitative evaluation of morphological change. Immunocytochemistry was applied to examine E-cadherion, a-SMA and FN proteins expression in the cells. Enzyme linked-immunosorbent assay was applied to MMP-9 and TIMP-1 levels. The results showed that EMT of A549 cells was induced by TGF-beta1, showing significant change of roundness, E-cadherion, alpha-SMA and FN (P < 0.05, P < 0.01). Compared to TGF-beta1, PNS significantly inhibited the changes of roundness (P < 0.05), FN and alpha-SMA (P < 0.05, P < 0.01) and not significantly inhibited the change of E-cadherion. Furthermore, MMP-9 levels were significantly increased by TGFbeta1 stimulation (P < 0.05), without significant change of TIMP-1. Compared with TGF-beta1, PNS could significantly increase MMP-9 level (P < 0.05) and decrease TIMP-1 levels (P < 0.05, P < 0.01). In conclusion, PNS could inhibit alveolar epithelial cell EMT induced by TGF-beta1, with increase of extracellular matrix degradation ability, which showed anti-fibrosis of lung ability.
Cell Proliferation
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drug effects
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Drugs, Chinese Herbal
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pharmacology
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Epithelial-Mesenchymal Transition
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drug effects
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Humans
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Matrix Metalloproteinase 9
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metabolism
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Panax notoginseng
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chemistry
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Pulmonary Alveoli
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cytology
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drug effects
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metabolism
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Saponins
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pharmacology
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Transforming Growth Factor beta1
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metabolism