1. Study on tripterin sustained-release dropping pills
Chinese Traditional and Herbal Drugs 2013;44(7):834-838
Objective: To prepare the Tripterin Sustained-release Dropping Pills (TSRDPs), investigate the disperse state and in vitro release, and optimize the preparation technology. Methods: The TSRDPs were prepared by solid dispersion method with PEG 4000 and glycerol monostearate (GM) as carrier materials. Orthogonal design was conducted to explore the influencing factors of the preparation technology by evaluating the indexes of roundness and weight difference. Results: The ideal condition of TSRDPs: the ratio of tripterin-GM-PEG 4000 was 1:3:7; the temperature of drug mixture was 80 °C; dropping speed was 20 d/min; dropping distance was 5 cm; and the condensate temperature was 15 °C. Tripterin existed as amorphous state in carriers. The maximum cumulative release amount of tripterin was 91.2% at 12 h. Conclusion: The prepared TSRDPs have a good sustained-release effect.
2.Risks of and response to cluster outbreak of imported malaria during malaria post-elimination era in China
ZHANG Li ; YIN Jian-hai ; XIA Zhi-gui
China Tropical Medicine 2023;23(6):585-
Abstract: Objective To analyze the recent cluster outbreaks of imported malaria and explore the risks, challenges and countermeasures for dealing with such events during malaria post-elimination era of malaria, and to provide reference for effectively addressing the risks and consolidating the achievements of malaria elimination. Methods The individual malaria case data from "The Information System for Infectious Disease Surveillance" and "The Information System For Parasitic Diseases Prevention And Control" were collected,and the diagnosis classification, infection source, time and space distribution of cases were analyzed. Results From January 1 to August 11, 2022, a total of 429 malaria cases were reported nationwide, an 18.9% decrease compared to the same period last year (529 cases), all of which were imported cases. The overall weekly trend of the outbreak remained stable, but since Week 31 (July 25-31), there has been a significant increase in the number of cases, with a peak on August 5. From July 25 to August 11, 2022, a total of 162 malaria cases were reported nationwide, up 315.4% from 39 cases in the same period last year, accounting for 37.8% of the total cases up to August 11, 2022. The main source of imported infections was Guinea (95 cases, 58.6%), with most cases reported in Longgang District, Shenzhen City, Guangdong Province (30 cases), Shilin County, Kunming City, Yunnan Province (21 cases), Chaoyang District, Beijing (11 cases), and Xiaoshan District, Hangzhou City, Zhejiang Province (7 cases). Conclusions Due to the concentration of returnees to China, several entry port cities simultaneously experienced cluster outbreaks of imported malaria, which brought immense pressure and challenges to local medical and health institutions. Health facilities at all levels need to maintain high vigilance and sensitivity, be well prepared, and avoid death and secondary transmission caused by imported cases.
3.Evaluation on contribution rate of each component total salvianolic acids and characterization of apparent oil/water partition coefficient.
Hong-mei YAN ; Xiao-yun CHEN ; Hai-jian XIA ; Dan LIU ; Xiao-bin JIA ; Zhen-hai ZHANG
China Journal of Chinese Materia Medica 2015;40(3):430-436
The difference between three representative components of total salvianolic acids in pharmacodynamic activity were compared by three different pharmacological experiments: HUVECs oxidative damage experiment, 4 items of blood coagulation in vitro experiment in rabbits and experimental myocardial ischemia in rats. And the effects of contribution rate of each component were calculated by multi index comprehensive evaluation method based on CRITIC weights. The contribution rates of salvianolic acid B, rosmarinic acid and Danshensu were 28.85%, 30.11%, 41.04%. Apparent oil/water partition coefficient of each representative components of total salvianolic acids in n-octyl alcohol-buffer was tested and the total salvianolic acid components were characterized based on a combination of the approach of self-defined weighting coefficient with effects of contribution rate. Apparent oil/water partition coefficient of total salvianolic acids was 0.32, 1.06, 0.89, 0.98, 0.90, 0.13, 0.02, 0.20, 0.56 when in octanol-water/pH 1.2 dilute hydrochloric acid solution/ pH 2.0, 2.5, 5.0, 5.8, 6.8, 7.4, 7.8 phosphate buffer solution. It provides a certain reference for the characterization of components.
Animals
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Benzofurans
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chemistry
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pharmacology
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Cinnamates
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chemistry
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pharmacology
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Depsides
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chemistry
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pharmacology
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Lactates
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chemistry
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pharmacology
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Male
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Rabbits
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Rats
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Rats, Sprague-Dawley
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Solubility
4.An overview of glycoconjugates for cancer targeting therapy and diagnosis.
Wei-Qin WANG ; Zhen-Hai ZHANG ; Jian-Ping ZHOU ; Hui PANG ; Hui-Xia LÜ
Acta Pharmaceutica Sinica 2013;48(12):1763-1770
Because of the changed metabolic behaviors of cancer cells, tumor cells uptake a corresponding larger amount of glucose in physiological condition when compared with normal cells. And they were prone to metabolize glucose for generating energy in anaerobic glycolysis ways in order to grow quickly. Anaerobic glycolysis consumes more glucose than aerobic way when the same amount of energy is obtained, which also results in large demand of glucose in tumor cells. This review briefly describes therapy methods related to characteristic mentioned above, and summarizes the research progress of drugs, diagnostic reagents and carriers conjugated with glucose, glucose derivatives or other kinds of sugars for cancer targeting. Furthermore, typically relative research reports from 2012 till now were listed and analyzed.
Animals
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Antineoplastic Agents
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therapeutic use
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Drug Carriers
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Energy Metabolism
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Glucose
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analogs & derivatives
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chemistry
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metabolism
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therapeutic use
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Glycoconjugates
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chemistry
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therapeutic use
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Glycolysis
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Glycosides
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chemistry
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Humans
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Ifosfamide
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analogs & derivatives
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chemistry
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therapeutic use
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Neoplasms
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diagnosis
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drug therapy
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metabolism
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Nitroimidazoles
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chemistry
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Radiation-Sensitizing Agents
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chemistry
6.Contrast study of assessment of Her2 gene by fluorescence in situ hybridization and immunohistochemistry technique in neoadjuvant chemotherapy breast cancer patients
Juan HUANG ; Xueli HU ; Jian HAI ; Feiyu CHEN ; Ting XIA ; Yaning HE ; Lili TANG
Journal of Chinese Physician 2012;14(1):5-8
ObjectiveTo evaluate the consistency of HER2 gene status before and after neoadjuvant chemotherapy in breast cancer by FISH (fluorescence in situ hybridization) and IHC (immunohistochemistry) techniques,and analyze the factor of the difference in the result and the feasibility of HER2 gene tested by FISH in the neoadjuvant chemotherapy breast cancer patients.Methods FISH and IHC for HER2 gene expression status was performed on the archival paraffin-embedded sections of breast cancer tissues before and after neoadjuvant chemotherapy from 135 Chinese female patients,x2 test of paired comparison of enumeration data and Kappa analysis were used to compare the difference and consistency of this two techniques.ResultsThe detection rate of HER2 status in punctured cancer tissues before neoadjuvant chemotherapy by FISH and HER2 did not show statistical difference in our research while the opposite result were showed in cancer tissues after neoadjuvant chemotherapy.Moreover,the two techniques of HER2 test were less concordant in patients accepted taxanes neoadjuvant chemotherapy than CAF treatment.ConclusionsThe consistency of FISH and IHC techniques of cancer tissues before neoadjuvant chemotherapy gained advantage compared to the ones after neoadjuvant chemotherapy.Patients received neoadjuvant chemotherapy especially taxanes should take the test of HER2 gene status by FISH technique.
7.The imaging study on the value of ~1H-MR spectroscopy in diffuse axonal injury
Hai-Jian XIA ; Xiao-Chuan SUN ; Wen-Yuan TANG ; Lv-Ping ZHENG ;
Chinese Journal of Radiology 2001;0(04):-
Objective To investigate the value of ~1H-MRS in the diagnosis and prognosis of diffuse axonal injury(DAI).Methods A prospective imaging study was performed in 63 patients with craniocerebral injury admitted from October 2002 to April 2004.Sixty-three patients were divided into DAI group(27 cases)and Non-DAI group(36 cases)according to the result of the MRI.Then,the ratio of NAA/Cr,Cho/Cr,mINs/Cr,and GIx/Cr at basal ganglia and genu and splenium of corpus callosum was quantified using ~1H-MRS and compared between DAI group and Non-DAI group.Twenty healthy persons were served as control group.The relation between ~1H-MRS indexes and period of primary uneonciousness post-injury was analyzed.Results The results of NAA/Cr and Cho/Cr at genu and splenium of corpus callosum and basal ganglia of control group were 1.19?0.18,1.21?0.24;1.89?0.17,1.84?0.14; 1.57?0.16,1.85?0.25,which of DAI group were 0.83?0.24,2.92?0.78;1.25?0.35,2.54? 0.42;1.33?0.17,2.38?0.44,and those of Non-DAI group were 1.11?0.23,1.61?0.33;1.61? 0.22,1.93?0.26;1.49?0.23,1.89?0.29.The differences between them were statistically significant (P
8.Establishing scientific evaluation view to promote traditional Chinese medicine
Xue-Min GAO ; Jian-Jun ZHANG ; Chun WANG ; Jing-Xia WANG ; Hai-Ying TONG ;
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(11):-
Traditional Chinese Medicine(TCM) makes great contributions to the prosperous growth and people's health.But understanding deviation and imperfect evaluation system of TCM affect the healthy development of TCM.Clinic practice is the motive power of TCM,and curative effect is the key of TCM researches,and the scientific evaluation system is the safeguard for a healthy development of TCM.So we should focus on clinical researches of stubborn diseases and emergency cases to satisfy social demand and upgrade the position of TCM in the medical system.At the same time,functional disease must be explored to show the advantage of TCM.Our mission is to establish a scientific objective evaluation system to accurately understand TCM and take it as the turning point to give an impetus to theoretical breakthrough of the basic studies to promote an overall and healthy development of TCM.
9.Isolation and Identification of Thermoacidiphilic Bacteria from Apple Juice Concentrate
Feng WANG ; Jian-Ke LI ; Yu-Rong GUO ; Hai-Xia LIU ;
Microbiology 2008;0(11):-
The thermotolerant bacteria which was isolated from the Apple Juice Concentrate(AJC) was investigated by comparison with the standard strain of Aliyclobacillus acidoterrestris DSM3922 using Kirin-medium acidified with malic acid.The results shows the two polluted bacteria isolated from AJC can grow under the temperature of 21?C~55?C and pH of 2.4~6.2,which was corresponded with the characteristics of the thermo-acidiphilic Alicyclobacillus spp..Further more,the cell morphology,colony morphology,cultural characteristics and physiological characteristics tests indicated the two isolated strains of thermotolerant bacteria have obviously similar characteristics with the standard strain of Aliyclobacillus acidoterrestris DSM3922.
10.Expression of interleukin-13 receptor and its relationship to proliferation activity of human gliomas
Zhi-Bai XIA ; Xin-Jian WU ; Tie-Wei QI ; Hai-Jun WANG ; Zheng-Song HUANG ;
Chinese Journal of Microsurgery 2006;0(06):-
Objective To explore the gene expression of interleukin-13 receptor (IL-13R)?2 and its relationship to proliferation activity of human gliomas.Methods The gene expression of IL-13R?in 50 hu- man gliomas,2 malignant human glioma cell lines and 6 normal brain tissues were studied by RT-PCR.Ki-67 labeling index (Ki267 LI) of all sample were detecteded by immunohistochemical staining.Results Only one normal brain tissues expressed very low IL-13R?2 mRNA,whereas 35 (70%) of 50 human brain tumors expressed 1L-13R?2 mRNA.The positive rate and expression level of IL-13R?2 mRNA were increased with the ascending of WHO tumor grade.(former:rs=0.87;letter:rs=0.69,P<0.01).The difference of posi- tive rate and expression level of IL-13R 2?mRNA between the low grade and high grade tumors was statistical- ly significant,the proliferation activity of gliomas evaluated by Ki-67LI (Ki-67 Labeling Index,Ki-67LI) was positively correlated with IL-13R?2 gene expression and the tumor grade.Conclusion In human cerebral gliomas,IL-13R?2 genes may play an role in the malignant progression.The expression level of malignancy in molecular level and selecting the target of gene therapy.