1.Study on fluoroquinolone resistance and the relationship between resistance and mutations of gyrA and parC in Neisseria gonorrhoeae.
Jin-shui XU ; Bei WANG ; Chang-xian WANG ; Hai-jian GOU ; Zu-huang MI ; Jian-hua LING
Chinese Journal of Epidemiology 2006;27(8):702-704
OBJECTIVETo study the phenotypic and genotypic resistance to Fluoroquinolones in Neisseria gonorrhoeae (NG) isolated in Jiangsu province of China.
METHODSIn-vitro, susceptibility testing of ciprofloxacin and levofloxacin against ninety-five clinical isolates were determined by agar dilution method. Detection of mutation in the gyrA and parC genes was performed by polymerase chain reaction (PCR) assay and sequence analysis.
RESULTSThe clinical isolates demonstrated 100% resistance to ciprofloxacin. Based on gyrA and parC mutations, 18 types could be categorized among the 54 isolates. Based on the same gyrA mutations,isolates with high MIC appeared to have had more mutations in parC gene.
CONCLUSIONThe status of resistance to ciprofloxacin in NG was quite serious, and ciprofloxacin treatment for the treatment of NG infections in Jiangsu province should not be recommended. The results from this study suggested that mutations in the parC gene had contributed to the development of high Fluoroquinolone resistance in NG.
China ; DNA Gyrase ; genetics ; DNA Topoisomerase IV ; genetics ; Drug Resistance, Bacterial ; Fluoroquinolones ; pharmacology ; Genotype ; Gonorrhea ; drug therapy ; Humans ; Neisseria gonorrhoeae ; drug effects ; genetics ; isolation & purification ; Phenotype
2.Progressive huge epidermoid cyst of distal femur in chronic osteomyelitis: a case report and review of literature.
Wei-Feng JI ; Pei-Jian TONG ; Zhen-Chuan MA ; Gui-Bao NI ; Gou-Hua SHEN ; Hai-Long ZHOU ; Xiao-Dong YAO ; Lu-Wei XIAO
China Journal of Orthopaedics and Traumatology 2011;24(12):1027-1029
Bone Diseases
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etiology
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surgery
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Chronic Disease
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Epidermal Cyst
;
etiology
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surgery
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Femur
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Humans
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Male
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Middle Aged
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Osteomyelitis
;
complications
3.Use of variable-number tandem repeats to examine genetic diversity of Bacillus anthracis.
Gou-zhong TAIN ; Rong HAI ; Dong-zheng YU ; Jian-Chun WEI ; Feng-Qin MA ; Hong CAI ; Jian-hua ZHANG ; Yu-hong ZHENG ; Xiu-ping FU ; Zhi-kai ZHANG ; En-min ZHANG ; Dong-lei XU
Chinese Journal of Epidemiology 2006;27(8):712-715
OBJECTIVETo study the genotyping of Bacillus anthracis based on multiple-locus variable-number tandem repeats(VNTR) in the B. anthracis genome.
METHODSWe selected 13 VNTR loci (which cited from published articles) to study 88 strains of B. anthracis isolated from China. The methods used were: (1) Selecting the primers which were at both ends of the tandem repeat locus; (2) Amplifying the sequence of the locus by PCR; (3)cDetecting the PCR products by agarose gel and polyacrylamide electrophoresis; (4)Analyzing the PCR products and computing the molecular weight by analysis software of gel images;(5) Double-checking with sequencing results; (6)Reckoning the repeat numbers and study the VNTRs loci characters.
RESULTS(1) We used multiple-locus variable-number tandem repeat analysis (MLVA) to characterize 88 B. anthracis isolates from diverse geographic locations which were divided into 45 MLVA genotypes and 3 groups through cluster analysis. The genotypes was relative to restricted geographical region. It seemed clear that the multiple isolates from the same anthrax outbreak frequently having identical genotypes. (2)Results from VNTR analysis showed that A16R vaccine strain isolated from China was having the nature of representativeness in the country.
CONCLUSIONAnalysis showed that the VNTR patterns was an appropriate study method for B. anthracis genetic diversity from different geographical areas and different time. Isolates from the same anthrax outbreak had identical
Anthrax ; epidemiology ; genetics ; Bacillus anthracis ; genetics ; isolation & purification ; China ; epidemiology ; Genetic Variation ; Genotype ; Geography ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Tandem Repeat Sequences