1.Analysis of infection after cesarean section
Haizhen ZHENG ; Caifei CHEN ; Hai HONG
Journal of Endocrine Surgery 2015;(3):227-229
Objective To analyze the causes of infection after cesarean section.Methods 80 patients having infection after cesarean section from Jan.2008 to Jan.2013 in our hospital were studied.The clinical data were retrospectively analyzed.The nosocomial infection-related factors were observed and related factors were ana-lyzed by Logistic regression analysis.Results 80 cases of maternal infection occurs after cesarean section.The highest rate of infection was urinary tract infection and respiratory infection, accounting for 43.75%and 31.25%respectively.We found the possible factors related to infection after cesarean section induding operative time, the number of invasive procedures, premature rupture of membranes, and the use of antibiotics.Conclusions Knowing the factors related to infection and improving the operative skills of gynecologists can help to reduce the rate of infection after cesarean section.
2.Effect of nitrogen, phosphorus, and potassium on yield and quality of Echinacea purpurea
Rong CHEN ; Hai NIAN ; Hong WU
Chinese Traditional and Herbal Drugs 1994;0(06):-
K. There was no significant difference in the yield among the treatments of N+K, CK, P, N+P, and N, but the results of these five treatments were significantly higher than that of P+K and K. In addition, cichoric acid content did not considerably changed after treatment of various fertilizer combinations. For the second harvest date the yield of N, N+K, P, and N+P were 47.7%, 35.4%, 33.8%, and 12.3% higher respectively than that of CK, the yield of N+P+K, P+K, and K were 7.7%, 10.8%, and 28.5% lower respectively than that of CK. There was significant difference in the yield between the treatment of N and CK, the yield of K was significantly lower than that of CK. Conclusion The results indicate that cichoric acid content is not significantly affected by the treatment of various fertilizer combinations and the yield is strongly influenced by N fertilizer, weakly by P fertilizer, on the contrary the application of potassium chloride results in a decrease in yield.
4.Susceptibility Testing of 28 Strains of Yeasts to the Combination of Antifungal Drugs in vitro
Hong CHEN ; Hai WEN ; Hong XU ; Wanqing LIAO
Chinese Journal of Dermatology 1995;0(03):-
Objective To study the interaction between antifungal drugs to yeasts in vitro.Methods The in vitro interaction of antifungal drugs was detected with checkerboard microdilution method based on M27-A of NCCLS for28strains of yeasts.Results There was a significant reduction in the geometric means of MICs by the combinations of antifungal drugs to the yeasts compared with the individual agents(P
5.Susceptibility testing of yeasts to combination of amphotericin B and 5-flucytosine in vitro according to M27-A method
Hong CHEN ; Hai WEN ; Hong XU ; Wanqing LIAO
Academic Journal of Second Military Medical University 1981;0(04):-
This article is to study the interaction between amphotericin B and 5-flucytosine to yeasts in vitro. The checkerboard microdilution method was applied to study in vitro interaction according to M27-A method of NCCLS. Twenty-eight strains of yeasts were used for combination study. Compared with the single agents,there were significant reductions in the geometric mean of the minimal inhibitory concentration of the combination(P
7.A preliminary study of the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin
Hong SANG ; Wanqing LIAO ; Jianghan CHEN ; Hai WEN
Journal of Medical Postgraduates 2001;14(2):103-105
Objectives:To study the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin.Methods:By comparing wild types(Mel+) and mutants(Mel-),we detected C.neoformans mutant laccase (CNLAC1) gene by PCR.We also cloned gene of the CNLAC1 containing about 450bp in length.E.coli DH5α was the host strain for the plasmid containing the DNA clone.Results:No deletion was found in wild type.However,CNLAC1 deletion (about 450 bp) was present (between 1 715 bp and 2 617 bp).CNLAC1 containing about 450 bp in length was cloned successfully.Conclusions:The present study was helpful in the biochemical and molecular study of CNLAC1 mutation.It might also provide us a target gene for the prognosis and gene therapy of Cryptocosis.
8.Cycloplegic effects of 0 . 5 g/L tropicamide in various low myopia age groups
Hai-Rong, ZHANG ; Hong-Xing, DIAO ; Xiang, CHEN
International Eye Science 2014;(12):2295-2296
AlM: To investigated the refraction and cycloplegic effects of 0. 5g/L tropicamide eye drops in various low myopia age groups, and provide clinical guidelines for proper usage of cyclopegic agent in different population.METHODS:Three low myopia age groups (n=45) were selected, non-cycloplegic and cycloplegic auto-refraction using “Shin-Nippon” auto-refractor were performed at various time point for refractive error. The subjective accommodation amplitudes were also collected at each time point.
RESULTS: The accommodation amplitude showed a similar but significant decline between 20~60min after the first cyclopegic drops in all three age groups. However, the decline of accommodation amplitudes were not statistically correlated with the results of “Shin-Nippon”auto-refraction results at different time points.
CONCLUSlON: The refraction time should be arranged according to the cycloplegic effects of tropicamide. Open-field auto-refractor could reduce the usage of cycloglegice drugs in low myopia groups, even for the youngest.
9.Clinical and Eletrophysiology Analysis of Infantile Spinal Muscular Atrophy
hai-hong, SUO ; lin, ZHAO ; wei, CHEN ; gui-rong, WANG
Journal of Applied Clinical Pediatrics 1993;0(03):-
Objective To explore the feature of electrical physiology and the key aspect of diagnosis in early stage of spinal muscular atrophy in infants.Method The back clinical data of the musle change of electrical physiology within 25 cases were analyzed.Results The children were ill in 1 year old,with the symptom that four limbs present symmetry and paralysing slowly,low limbs being more serious than upper limbs,the close and the distant of the four limbs.The levels of creatine kinase(CK) and lactate dehydrogenase(LDH) in serum were normal and the case unconcentrated was not seen rarely.The show of electromyogram(EMG) was that three limbs presenting wide-range losing nerve electric potential in peace state,when straingently the deadline was long and the motor unit potential(MUP) was high,when strain highly the eletric potential was decreased;the speed of nerve conduction was common.Muscle inspection was typical nervine myatrophy.Conclusion The clinical feature,the changes of muscle electrical physiology and the muscle inspection are valuable for diagnosis so as to afford reliable evidence for further gene diagnosis.J Appl Clin Pediatr,2006,21(3):167
10.Research and Progress on Feed Phytase Reform by Protein Engineering
Hui CHEN ; Hong-Ning WANG ; Qi WU ; Hai-Xia ZHAO ;
Microbiology 1992;0(03):-
As a kind of additive in feed of monogastric animals, the application of natural phytase is limited due to its disadvantages. In this paper, the strategies of phytse reform was introduced. Furthermore, the research and progress on protein engineering of feed phytase was reviewed, including phytase over-expression, phytase thermostability, catalytic efficiency and optimum pH.