1.Recent researching progress of Lepidium meyenii (Maca).
Yan-yan ZHOU ; Hai-yu ZHAO ; Nan SI ; Hong-jie WANG ; Bao-lin GIAN
China Journal of Chinese Materia Medica 2015;40(23):4521-4530
Maca as one of the star products in the international health care market in recent years, had a wide range of application value and promoted to all over the world. However, the basic research of Maca was not deep, lack of systematic and clear efficacy studies. Market products hype its aphrodisiac effect, which greatly impact more systematic in-depth research and exploration. Therefore, this paper briefly summarizes advance research in recent years including the status quo of the resources, growth cultivation, phytochemical, pharmacological effect and other aspects, which can provide reference for rational development and utilization of Maca.
Animals
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Biomedical Research
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Humans
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Lepidium
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chemistry
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classification
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growth & development
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Plant Extracts
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chemistry
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metabolism
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pharmacology
2.Cervical carcinoid with high-grade intraepithelial neoplasia: report of a case.
Hai LI ; Fang BAO ; Yu-fei LI ; Yi-long DAI ; Ying XIANG ; Zhi-hong ZHANG
Chinese Journal of Pathology 2013;42(5):347-348
Adult
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Breast Neoplasms
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metabolism
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pathology
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secondary
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Carcinoid Tumor
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metabolism
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pathology
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surgery
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Carcinoma, Adenoid Cystic
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pathology
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Carcinoma, Lobular
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metabolism
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pathology
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secondary
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Cervical Intraepithelial Neoplasia
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metabolism
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pathology
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surgery
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Chromogranin A
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metabolism
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Diagnosis, Differential
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Female
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Humans
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Hysterectomy
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Keratins
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metabolism
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Neoplasms, Multiple Primary
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metabolism
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pathology
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surgery
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Ovarian Neoplasms
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metabolism
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pathology
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Sex Cord-Gonadal Stromal Tumors
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metabolism
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pathology
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Synaptophysin
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metabolism
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Uterine Cervical Neoplasms
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metabolism
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pathology
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surgery
3.Identification of bufadienolides profiling in cinobufacino by HPLC-DAD-FT-ICR-MS method.
Jun-Qiu LIU ; Nan SI ; Jian YANG ; Hai-Yu ZHAO ; Bao-Lin BIAN ; Hong-Jie WANG
Acta Pharmaceutica Sinica 2014;49(2):244-248
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC-DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.
Amphibian Venoms
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chemistry
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Animals
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Bufanolides
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analysis
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chemistry
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Bufo bufo
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Chromatography, High Pressure Liquid
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Molecular Structure
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Spectrometry, Mass, Electrospray Ionization
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Tandem Mass Spectrometry
4.LC-FT-ICR-MS analysis of the prototypes and metabolites in rat plasma after administration of huang-lian-jie-du decoction.
Ran ZUO ; Hong-Jie WANG ; Nan SI ; Hai-Yu ZHAO ; Jian YANG ; Bao-Lin BIAN
Acta Pharmaceutica Sinica 2014;49(2):237-243
To obtain chemical constituent information of rat plasma after oral administration of Huang-Lian-Jie-Du Decoction (HLJDD), a LC-FT-ICR-MS method has been established, and both positive and negative ions scan modes were include in the analysis. By comparing their retention time, high resolution mass data of HLJDD extracts, blank plasma and dosed plasma, 38 constituents, including 22 prototype compounds and 16 metabolites, were detected in rat plasma after oral administration of HLJDD. In the 22 prototype compounds, 16 constituents were determined unambiguously by comparing with references. In the analysis of metabolites, phase II reactions like glucuronidation and sulfation were the major biotransformation pathways of HLJDD. M11 was observed as the only phase I metabolite in present experiment. The results will be beneficial for the further pharmacokinetics and pharmacological evaluations of HLJDD.
Administration, Oral
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Alkaloids
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blood
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Animals
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Biotransformation
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Chromatography, High Pressure Liquid
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Drug Combinations
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Drugs, Chinese Herbal
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administration & dosage
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isolation & purification
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metabolism
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Flavonoids
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blood
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Iridoids
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blood
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Male
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Plants, Medicinal
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chemistry
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Rats
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Rats, Sprague-Dawley
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Spectrometry, Mass, Electrospray Ionization
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Tandem Mass Spectrometry
5.3D reconstruction of the heart model based on the region growing segmentation.
Dan-hong XU ; Bao-hua WANG ; Yong ZHANG ; Hai-dong SHENG ; You-li YE
Chinese Journal of Medical Instrumentation 2007;31(1):17-21
The technique introduced in this paper is applied in the endocardial catheter operation, which describes the 3D heart model reconstruction before the operation for the endocardial navigation. After a series of CT images of the thorax are processed, an accurate 3D endocardial model can be reconstructed. At first, the series of 2D CT images are preprocessed for denoising and the enhancement,then they are constructed as the volume data. After the region growing segmentation in the 3D volume data according to the grey value of the voxel in the heart cavity, the heart surface rendering is got and the 3D model of endocardial cavity is reconstructed.
Cardiac Catheterization
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methods
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Imaging, Three-Dimensional
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Models, Cardiovascular
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Tomography, X-Ray Computed
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methods
6.Diagnostic Value of Computed Radiography on Neonatal Respiratory Distress Syndrome
ji-cheng, DU ; hai-bin, ZHOU ; fu-chun, LI ; rui-zhen, HONG ; man-hua, BAO
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To improve the knowledge and diagnostic ability of imagiology on neonatal respiratory distress syndrome (NRDS) computed radiograph(CR).Methods The doubtful patients were done to photographs bedside using the high resolution imaging plate, 50 cases of newborn with NRDS were selected whose clinical diagnosed clearly and had been treated and had the complete CR image documents.The CR change and clinical characteristics were observed dynamically.Results Nine of 50 cases were combined with aspirated pneumonia,8 cases with infective pneumonia,3 cases with intra-alveolar hemorrage,and 2 cases with pneumothorax.Accoding to X-ray manifestations,all cases were divided into four stages:Ⅰ stage(n=5), Ⅱ stage(n=20),Ⅲ stage(n=22),Ⅳstage(n=3).Typical CR signs included:the pulmonary lucency decreasd,wide-ranging net and grain shadowes of high density, and in companing with a lot of air brunchus sing.Conclusions Computed radiography is the most important imaging method in diagnosis of NRDS bedside ,and shall be improved the ability of diagnosis and differential of NRDS combined with the clinic.
7.Determination of oxide, furan, dichloromethane by portable gas chromatography.
Zheng RUAN ; Hong-fang TANG ; Dan-hua LIU ; Hai-bao ZHU ; Han WANG ; Ya-ling QIAN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(11):868-870
Air
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analysis
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Chromatography, Gas
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methods
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Ethylene Oxide
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analysis
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Furans
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analysis
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Methylene Chloride
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analysis
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Workplace
8.Chemical constituents from Lyophyllum decastes.
Hong-Liang ZHENG ; Tolgor BAU ; Hai-Ying BAO ; Jun-Wen LIAN
China Journal of Chinese Materia Medica 2013;38(24):4335-4339
The chemical constituents from the fruiting bodies of Lyophyllum decastes (Fr.) Singer were studied in this paper. Thirteen compounds were isolated and purified by column chromatographies on silica gel and Sephadex LH-20. Their structures were identified by MS and NMR data analysis as adenosine (1), 2R, 3S, 4S, 8E)-2-[(2'R)-2-hydroxyheneicosanoylamino]-8-octadecene-1, 3, 4-triol (2), (2R, 3S, 4S, 8E)-2-[(2'R)-2-hydroxypentacosanoylamino]-8-octadecene-1, 3, 4-triol (3), nicotinic acid (4), (4E, 8E) -2-N-2-hydroxytetracosanoyl-1-O-beta-D-glycopyranosyl-9-methyl-4, 8-sphingadienine (5), D-mannitol (6), ergosteryl-3-O-beta-D-glucopyranoside (7), tuberoside (8), (2R, 3S, 4S, 8E)-2-[(2'R)-2-hydroxybehenoylamino]-8-octadecene-1, 3, 4-triol (9),(2R, 3S, 4S, 8E)-2-[(2'R) -2-hydroxytricosanoylamino] -8-octadecene-1, 3, 4-triol (10), (22E, 24R)-ergosta-7, 22-dien-3beta, 5alpha, 6beta-triol (11), (22E, 24R)-ergosta-5, 7, 22-trien-3beta-ol (12), and 5alpha, 8alpha-epidiory-(22E, 24R)-ergosta-6, 22-dien-3beta-ol (13), respectively. All the above compounds are first obtained from the mushroom and compounds 2-10 are reported to be obtained from the Lyophyllum for the first time.
Agaricales
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chemistry
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Drugs, Chinese Herbal
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chemistry
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Fruit
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chemistry
9.Multiplicity Teaching on Microbiology Laboratory Class
Ling-Ling WANG ; Ling-Hua ZHANG ; Bao-Sheng LI ; Hai-Hong WANG ;
Microbiology 2008;0(07):-
Laboratory teaching played the crucial part of microbiology teaching, which was benefit to improve the operational capacity, analysis and resolving ability of students. We carried out the multiplicity teaching by remodeling experiment process, designing integral test, and resolving practical issue and setting up comprehensive trail. It was proved by fact that the multiplicity teaching increased the study interesting of students, inspired their activity, initiative and creativity.
10.Sarcoidosis of kidney: report of a case.
Qiong-zhen LIN ; Li-hong ZHANG ; Hai-ying LIN ; Yan-qing CHI ; Bao-xing WANG ; Ying LI ; Wan-zhong ZOU
Chinese Journal of Pathology 2007;36(1):62-63
Adult
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Biopsy, Needle
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Diagnosis, Differential
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Humans
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Kidney
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pathology
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Kidney Diseases
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pathology
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therapy
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Male
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Nephritis, Interstitial
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pathology
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Renal Dialysis
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Sarcoidosis
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pathology
;
therapy
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Tuberculosis, Renal
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pathology